P35774 (SPIKE_ADE1P) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fiber protein
Short name=SPIKE

Alternative name(s):
Protein IV

Gene names

ORF Names:

L5

Organism

Human adenovirus B serotype 11 (strain Slobiski) (HAdV-11) (Human adenovirus 11P (strain Slobiski))

Taxonomic identifier

343462 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus › Human adenovirus B › Human adenovirus B2 › Human adenovirus 11

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Forms spikes that protrude from each vertex of the icosahedral capsid. Interacts with host receptor CD46 to provide virion initial attachment to target cell. Fiber proteins are shed during virus entry, when virus is still at the cell surface.
Subunit structure	Homotrimer. Interacts with host receptor CD46. Interacts (via N-terminal tail region) with pentons By similarity.
Subcellular location	Virion By similarity. Host nucleus By similarity. Note: Anchored to the pentons, protrudes from the virion surface By similarity.
Induction	Expressed in the late phase of the viral replicative cycle.
Domain	The tail region anchors the fiber to penton base capsomers, whereas the shaft, built from several repeated motifs, allows the knob to protrude from the virion By similarity.
Miscellaneous	All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell By similarity.
Sequence similarities	Belongs to the adenoviridae fiber family.

Ontologies

Keywords
Biological process	Host-virus interaction Viral attachment to host cell Virus entry into host cell
Cellular component	Host nucleus Virion
Developmental stage	Late protein
Molecular function	Capsid protein
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: InterPro viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 325

325

Fiber protein

PRO_0000221794

Secondary structure

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325

Helix Strand Turn

Details...

Helix

130 – 132

3

Beta strand

133 – 135

3

Beta strand

151 – 153

3

Beta strand

156 – 165

10

Beta strand

168 – 177

10

Helix

180 – 183

4

Helix

184 – 187

4

Beta strand

189 – 199

11

Turn

207 – 209

3

Beta strand

210 – 212

3

Helix

232 – 235

4

Turn

239 – 241

3

Helix

247 – 253

7

Beta strand

254 – 262

9

Beta strand

268 – 279

12

Beta strand

283 – 285

3

Beta strand

288 – 297

10

Turn

305 – 307

3

Beta strand

316 – 322

7

Sequences

	Sequence		Length	Mass (Da)
	P35774 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 3B3ACCA4CBDB3EFD	FASTA	325	35,538
	10 20 30 40 50 60 MTKRVRLSDS FNPVYPYEDE STSQHPFINP GFISPNGFTQ SPNGVLTLKC LTPLTTTGGS 70 80 90 100 110 120 LQLKVGGGLT VDDTNGFLKE NISATTPLVK TGHSIGLPLG AGLGTNENKL CIKLGQGLTF 130 140 150 160 170 180 NSNNICIDDN INTLWTGVNP TEANCQIMNS SESNDCKLIL TLVKTGALVT AFVYVIGVSN 190 200 210 220 230 240 NFNMLTTHRN INFTAELFFD STGNLLTRLS SLKTPLNHKS GQNMATGAIT NAKGFMPSTT 250 260 270 280 290 300 AYPFNDNSRE KENYIYGTCY YTASDRTAFP IDISVMLNRR AINDETSYCI RITWSWNTGD 310 320 APEVQTSATT LVTSPFTFYY IREDD

References

[1]	"Hemagglutination properties and nucleotide sequence analysis of the fiber gene of adenovirus genome types 11p and 11a." Mei Y.-F., Wadell G. Virology 194:453-462(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Adenovirus receptors." Zhang Y., Bergelson J.M. J. Virol. 79:12125-12131(2005) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF532578 Genomic DNA. Translation: AAA42490.1.

PIR

D37476.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2O39	X-ray	2.85	A/B	129-325	[»]
3EXV	X-ray	1.45	A	117-325	[»]

ProteinModelPortal

P35774.

SMR

P35774. Positions 129-325.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.10.25.20. 1 hit.
2.60.90.10. 1 hit.

InterPro

IPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
[Graphical view]

Pfam

PF00541. Adeno_knob. 1 hit.
PF00608. Adeno_shaft. 2 hits.
[Graphical view]

PRINTS

PR00307. ADENOVSFIBRE.

SUPFAM

SSF49835. Viral_att. 1 hit.
SSF51225. Viral_att_shaft. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P35774.

Entry information

Entry name

SPIKE_ADE1P

Accession

Primary (citable) accession number: P35774

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program