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Protein

Fiber protein

Gene

L5

Organism
Human adenovirus B serotype 11 (strain Slobiski) (HAdV-11) (Human adenovirus 11P (strain Slobiski))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Forms spikes that protrude from each vertex of the icosahedral capsid. Interacts with host receptor CD46 to provide virion initial attachment to target cell. Fiber proteins are shed during virus entry, when virus is still at the cell surface.

GO - Biological processi

  1. cell adhesion Source: InterPro
  2. pathogenesis Source: InterPro
  3. viral entry into host cell Source: UniProtKB-KW
  4. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral attachment to host adhesion receptor, Viral attachment to host cell, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Fiber protein
Short name:
SPIKE
Alternative name(s):
Protein IV
Gene namesi
ORF Names:L5
OrganismiHuman adenovirus B serotype 11 (strain Slobiski) (HAdV-11) (Human adenovirus 11P (strain Slobiski))
Taxonomic identifieri343462 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirusHuman mastadenovirus B
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

Virion By similarity. Host nucleus By similarity
Note: Anchored to the pentons, protrudes from the virion surface.By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-SubCell
  2. viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325Fiber proteinPRO_0000221794Add
BLAST

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homotrimer. Interacts with host receptor CD46. Interacts (via N-terminal tail region) with pentons (By similarity).By similarity

Structurei

Secondary structure

1
325
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi130 – 1323Combined sources
Beta strandi133 – 1353Combined sources
Beta strandi151 – 1533Combined sources
Beta strandi156 – 16510Combined sources
Beta strandi168 – 17710Combined sources
Helixi180 – 1834Combined sources
Helixi184 – 1874Combined sources
Beta strandi189 – 19911Combined sources
Turni207 – 2093Combined sources
Beta strandi210 – 2123Combined sources
Helixi232 – 2354Combined sources
Turni239 – 2413Combined sources
Helixi247 – 2537Combined sources
Beta strandi254 – 2629Combined sources
Beta strandi268 – 27912Combined sources
Beta strandi283 – 2853Combined sources
Beta strandi288 – 29710Combined sources
Turni305 – 3073Combined sources
Beta strandi316 – 3227Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2O39X-ray2.85A/B129-325[»]
3EXVX-ray1.45A117-325[»]
ProteinModelPortaliP35774.
SMRiP35774. Positions 129-325.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35774.

Family & Domainsi

Domaini

The tail region anchors the fiber to penton base capsomers, whereas the shaft, built from several repeated motifs, allows the knob to protrude from the virion.By similarity

Sequence similaritiesi

Belongs to the adenoviridae fiber family.Curated

Family and domain databases

Gene3Di2.10.25.20. 1 hit.
2.60.90.10. 1 hit.
InterProiIPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
[Graphical view]
PfamiPF00541. Adeno_knob. 1 hit.
PF00608. Adeno_shaft. 2 hits.
[Graphical view]
PRINTSiPR00307. ADENOVSFIBRE.
SUPFAMiSSF49835. SSF49835. 1 hit.
SSF51225. SSF51225. 1 hit.

Sequencei

Sequence statusi: Complete.

P35774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKRVRLSDS FNPVYPYEDE STSQHPFINP GFISPNGFTQ SPNGVLTLKC
60 70 80 90 100
LTPLTTTGGS LQLKVGGGLT VDDTNGFLKE NISATTPLVK TGHSIGLPLG
110 120 130 140 150
AGLGTNENKL CIKLGQGLTF NSNNICIDDN INTLWTGVNP TEANCQIMNS
160 170 180 190 200
SESNDCKLIL TLVKTGALVT AFVYVIGVSN NFNMLTTHRN INFTAELFFD
210 220 230 240 250
STGNLLTRLS SLKTPLNHKS GQNMATGAIT NAKGFMPSTT AYPFNDNSRE
260 270 280 290 300
KENYIYGTCY YTASDRTAFP IDISVMLNRR AINDETSYCI RITWSWNTGD
310 320
APEVQTSATT LVTSPFTFYY IREDD
Length:325
Mass (Da):35,538
Last modified:June 1, 1994 - v1
Checksum:i3B3ACCA4CBDB3EFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF532578 Genomic DNA. Translation: AAA42490.1.
PIRiD37476.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF532578 Genomic DNA. Translation: AAA42490.1.
PIRiD37476.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2O39X-ray2.85A/B129-325[»]
3EXVX-ray1.45A117-325[»]
ProteinModelPortaliP35774.
SMRiP35774. Positions 129-325.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP35774.

Family and domain databases

Gene3Di2.10.25.20. 1 hit.
2.60.90.10. 1 hit.
InterProiIPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
[Graphical view]
PfamiPF00541. Adeno_knob. 1 hit.
PF00608. Adeno_shaft. 2 hits.
[Graphical view]
PRINTSiPR00307. ADENOVSFIBRE.
SUPFAMiSSF49835. SSF49835. 1 hit.
SSF51225. SSF51225. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Hemagglutination properties and nucleotide sequence analysis of the fiber gene of adenovirus genome types 11p and 11a."
    Mei Y.-F., Wadell G.
    Virology 194:453-462(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: REVIEW.

Entry informationi

Entry nameiSPIKE_ADE1P
AccessioniPrimary (citable) accession number: P35774
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: January 7, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell (By similarity).By similarity

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.