P35762 (CD81_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CD81 antigen

Alternative name(s):
26 kDa cell surface protein TAPA-1
Target of the antiproliferative antibody 1
CD_antigen=CD81

Gene names

Name:	Cd81
Synonyms:	Tapa1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play an important role in the regulation of lymphoma cell growth. May be involved in the acrosome reaction. Ref.12
Subunit structure	Interacts with CD53 and SCIMP By similarity. Interacts directly with IGSF8. Interacts with IFITM1 By similarity. Interacts with IFITM2 and IFITM3. Ref.9 Ref.11
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in oocytes. Highly expressed in granulosa cells. Ref.10 Ref.12
Post-translational modification	Not glycosylated Probable.
Disruption phenotype	Reduction in the fecundity of female mice and their eggs have impaired ability to fuse with sperm. Ref.10 Ref.12
Sequence similarities	Belongs to the tetraspanin (TM4SF) family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	activation of MAPK activity Inferred from sequence or structural similarity. Source: UniProtKB phosphatidylinositol biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of 1-phosphatidylinositol 4-kinase activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of B cell proliferation Traceable author statement PubMed 14676841. Source: UniProtKB positive regulation of cell growth Traceable author statement PubMed 14676841. Source: UniProtKB positive regulation of peptidyl-tyrosine phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB protein localization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	immunological synapse Inferred from electronic annotation. Source: Compara integral to plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	CD81 antigen		PRO_0000219222
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 33	21	Helical; Potential
	Topological domain	34 – 57	24	Extracellular Potential
	Transmembrane	58 – 78	21	Helical; Potential
	Topological domain	79 – 89	11	Cytoplasmic Potential
	Transmembrane	90 – 115	26	Helical; Potential
	Topological domain	116 – 201	86	Extracellular Potential
	Transmembrane	202 – 226	25	Helical; Potential
	Topological domain	227 – 236	10	Cytoplasmic Potential
Amino acid modifications
	Disulfide bond	156 ↔ 190		By similarity
	Disulfide bond	157 ↔ 175		By similarity
Experimental info
	Sequence conflict	173	1	S → T in AAB19417. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P35762 [UniParc]. Last modified October 3, 2012. Version 2. Checksum: 7BDCB792E7F9FA4E	FASTA	236	25,815
	10 20 30 40 50 60 MGVEGCTKCI KYLLFVFNFV FWLAGGVILG VALWLRHDPQ TTSLLYLELG NKPAPNTFYV 70 80 90 100 110 120 GIYILIAVGA VMMFVGFLGC YGAIQESQCL LGTFFTCLVI LFACEVAAGI WGFVNKDQIA 130 140 150 160 170 180 KDVKQFYDQA LQQAVMDDDA NNAKAVVKTF HETLNCCGSN ALTTLTTTIL RNSLCPSGGN 190 200 210 220 230 ILTPLLQQDC HQKIDELFSG KLYLIGIAAI VVAVIMIFEM ILSMVLCCGI RNSSVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic organization and chromosomal localization of the TAPA-1 gene." Andria M.L., Hsieh C.L., Oren R., Francke U., Levy S. J. Immunol. 147:1030-1036(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence conservation and variability of imprinting in the Beckwith-Wiedemann syndrome gene cluster in human and mouse." Paulsen M., El-Maarri O., Engemann S., Stroedicke M., Franck O., Davies K., Reinhardt R., Reik W., Walter J. Hum. Mol. Genet. 9:1829-1841(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NOD. Tissue: Mammary gland.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[7]	Duff K., Parsons J. Submitted (APR-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-236. Tissue: Heart.
[8]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 125-144; 149-171 AND 194-201, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[9]	"PGRL is a major CD81-associated protein on lymphocytes and distinguishes a new family of cell surface proteins." Clark K.L., Zeng Z., Langford A.L., Bowen S.M., Todd S.C. J. Immunol. 167:5115-5121(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH IGSF8.
[10]	"Reduced fertility of female mice lacking CD81." Rubinstein E., Ziyyat A., Prenant M., Wrobel E., Wolf J.-P., Levy S., Le Naour F., Boucheix C. Dev. Biol. 290:351-358(2006) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
[11]	"Expression of the mouse fragilis gene products in immune cells and association with receptor signaling complexes." Smith R.A., Young J., Weis J.J., Weis J.H. Genes Immun. 7:113-121(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH IFITM2 AND IFITM3.
[12]	"Possible involvement of CD81 in acrosome reaction of sperm in mice." Tanigawa M., Miyamoto K., Kobayashi S., Sato M., Akutsu H., Okabe M., Mekada E., Sakakibara K., Miyado M., Umezawa A., Miyado K. Mol. Reprod. Dev. 75:150-155(2008) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, FUNCTION IN ACROSOME REACTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S45012 , S44957, S44966, S45001, S45008, S45010 Genomic DNA. Translation: AAB19417.1. AJ251835 Genomic DNA. Translation: CAB94774.1. AK166521 mRNA. Translation: BAE38825.1. AK170741 mRNA. Translation: BAE41994.1. AC015800 Genomic DNA. No translation available. CH466531 Genomic DNA. Translation: EDL18195.1. BC011433 mRNA. Translation: AAH11433.1. X59047 mRNA. No translation available.
IPI	IPI00310916. IPI00405736.
PIR	A46472.
RefSeq	NP_598416.1. NM_133655.2.
UniGene	Mm.806.
3D structure databases
HSSP	HSSP built from PDB template 1G8Q based on UniProtKB P60033.
ProteinModelPortal	P35762.
SMR	P35762. Positions 113-201.
ModBase	Search...
PTM databases
PhosphoSite	P35762.
Proteomic databases
PaxDb	P35762.
PRIDE	P35762.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000037941; ENSMUSP00000043768; ENSMUSG00000037706.
GeneID	12520.
KEGG	mmu:12520.
Organism-specific databases
CTD	975.
MGI	MGI:1096398. Cd81.
Phylogenomic databases
eggNOG	NOG305712.
GeneTree	ENSGT00680000099842.
HOGENOM	HOG000230651.
HOVERGEN	HBG002324.
InParanoid	P35762.
KO	K06508.
OMA	CHQKIDE.
OrthoDB	EOG4X97J1.
Gene expression databases
CleanEx	MM_CD81.
Genevestigator	P35762.
GermOnline	ENSMUSG00000037706. Mus musculus.
Family and domain databases
InterPro	IPR000301. Tetraspanin. IPR018499. Tetraspanin/Peripherin. IPR018503. Tetraspanin_CS. IPR008952. Tetraspanin_EC2. [Graphical view]
Pfam	PF00335. Tetraspannin. 1 hit. [Graphical view]
PIRSF	PIRSF002419. Tetraspanin. 1 hit.
PRINTS	PR00259. TMFOUR.
SUPFAM	SSF48652. Tetraspanin. 1 hit.
PROSITE	PS00421. TM4_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CD81. mouse.
NextBio	281526.
SOURCE	Search...

Entry information

Entry name

CD81_MOUSE

Accession

Primary (citable) accession number: P35762
Secondary accession number(s): Q91V78

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program