Reviewed,
UniProtKB/Swiss-Prot P35761 (TTK_MOUSE)
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November 3, 2009.
Version 74.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Dual specificity protein kinase TTK Short name=ESK Short name=PYT EC=2.7.12.1 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 856 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint By similarity. Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. May play some role in the control of cell proliferation or differentiation and could be involved in modulating different levels of signal transduction pathways. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Enzyme regulation | Inhibited by the ATP-competitive kinase inhibitor, SP600125 By similarity. |
| Tissue specificity | Present in rapidly proliferating cell lines; high levels in testis, bone marrow, spleen and thymus. Low levels in brain, heart, lung and kidney. |
| Post-translational modification | Autophosphorylated. Ref.2 |
| Sequence similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activityInferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P35761-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P35761-2) The sequence of this isoform differs from the canonical sequence as follows: 296-321: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 856 | 856 | Dual specificity protein kinase TTK | PRO_0000086775 | |||||
Regions | |||||||||
| Domain | 524 – 790 | 267 | Protein kinase | ||||||
| Nucleotide binding | 530 – 538 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 646 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 552 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 32 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 36 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 277 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 342 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 428 | 1 | Phosphothreonine Ref.2 | ||||||
| Modified residue | 435 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 820 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 823 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 296 – 321 | 26 | Missing in isoform 2. | VSP_004875 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Multiple cDNAs encoding the esk kinase predict transmembrane and intracellular enzyme isoforms." Douville E.M.J., Afar D.E.H., Howell B.W., Letwin K., Tannock L., Ben-David Y., Pawson T., Bell J.C. Mol. Cell. Biol. 12:2681-2689(1992) [PubMed: 1375325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). |
| [2] | "Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 18973353] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-428, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M86377 mRNA. Translation: AAA37578.1. | |
| IPI | IPI00122018. IPI00762064. |
| PIR | A44439. B44439. |
| UniGene | Mm.1904 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1BI8 based on UniProtKB Q00534. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | P35761. |
Proteomic databases | |
| PRIDE | P35761. |
Genome annotation databases | |
| Ensembl | ENSMUST00000070326; ENSMUSP00000064839; ENSMUSG00000038379; Mus musculus. [Genome view] |
| UCSC | uc009qwp.1. mouse. |
Organism-specific databases | |
| MGI | MGI:1194921. Ttk. |
Phylogenomic databases | |
| HOVERGEN | P35761. |
| OMA | LEAVHII. |
Enzyme and pathway databases | |
| BRENDA | 2.7.12.1. 244. |
Gene expression databases | |
| CleanEx | MM_TTK. |
| Genevestigator | P35761. |
Family and domain databases | |
| InterPro | IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| SOURCE | Search... |
Entry information
| Entry name | TTK_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P35761 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |
