P35761 (TTK_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 100.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Dual specificity protein kinase TTK EC=2.7.12.1 Alternative name(s): ESK PYT | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) [Reference proteome] | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 856 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint By similarity. Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. May play some role in the control of cell proliferation or differentiation and could be involved in modulating different levels of signal transduction pathways. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Enzyme regulation | Inhibited by the ATP-competitive kinase inhibitor, SP600125 By similarity. |
| Subunit structure | Interacts with TPR; the interactions occurs in a microtubule-independent manner By similarity. |
| Tissue specificity | Present in rapidly proliferating cell lines; high levels in testis, bone marrow, spleen and thymus. Low levels in brain, heart, lung and kidney. |
| Post-translational modification | Autophosphorylated. |
| Sequence similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase |
| PTM | Acetylation Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Molecular_function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activityInferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine/tyrosine kinase activityInferred from electronic annotation. Source: EC protein tyrosine kinase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P35761-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P35761-2) The sequence of this isoform differs from the canonical sequence as follows: 296-321: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 856 | 856 | Dual specificity protein kinase TTK | PRO_0000086775 | |||||
Regions | |||||||||
| Domain | 524 – 790 | 267 | Protein kinase | ||||||
| Nucleotide binding | 530 – 538 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 646 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 552 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1 | 1 | N-acetylmethionine By similarity | ||||||
| Modified residue | 32 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 36 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 277 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 342 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 428 | 1 | Phosphothreonine Ref.2 | ||||||
| Modified residue | 435 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 820 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 296 – 321 | 26 | Missing in isoform 2. | VSP_004875 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Multiple cDNAs encoding the esk kinase predict transmembrane and intracellular enzyme isoforms." Douville E.M.J., Afar D.E.H., Howell B.W., Letwin K., Tannock L., Ben-David Y., Pawson T., Bell J.C. Mol. Cell. Biol. 12:2681-2689(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). |
| [2] | "Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-428, MASS SPECTROMETRY. Tissue: Melanoma. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M86377 mRNA. Translation: AAA37578.1. |
| IPI | IPI00122018. IPI00762064. |
| PIR | A44439. B44439. |
| UniGene | Mm.1904. |
3D structure databases | |
| ProteinModelPortal | P35761. |
| SMR | P35761. Positions 55-191, 460-821. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P35761. 2 interactions. |
| STRING | 10090.ENSMUSP00000064839. |
PTM databases | |
| PhosphoSite | P35761. |
Proteomic databases | |
| PaxDb | P35761. |
| PRIDE | P35761. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000070326; ENSMUSP00000064839; ENSMUSG00000038379. |
| UCSC | uc009qwo.2. mouse. |
Organism-specific databases | |
| MGI | MGI:1194921. Ttk. |
Phylogenomic databases | |
| eggNOG | COG0515. |
| GeneTree | ENSGT00620000087848. |
| HOGENOM | HOG000013174. |
| HOVERGEN | HBG001029. |
| OMA | MSCFRTP. |
| OrthoDB | EOG4JM7PB. |
Enzyme and pathway databases | |
| BRENDA | 2.7.12.1. 3474. |
Gene expression databases | |
| Bgee | P35761. |
| CleanEx | MM_TTK. |
| Genevestigator | P35761. |
Family and domain databases | |
| Gene3D | 1.25.40.10. 1 hit. |
| InterPro | IPR027084. Dual_sp_prot_kinse_Ttk. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. IPR011990. TPR-like_helical. [Graphical view] |
| PANTHER | PTHR22974:SF4. PTHR22974:SF4. 1 hit. |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| SUPFAM | SSF56112. Kinase_like. 1 hit. |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| SOURCE | Search... |
Entry information
| Entry name | TTK_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P35761 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |