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Protein

Dual specificity protein kinase TTK

Gene

Ttk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint (By similarity). Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. May play some role in the control of cell proliferation or differentiation and could be involved in modulating different levels of signal transduction pathways.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Inhibited by the ATP-competitive kinase inhibitor, SP600125.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521ATPPROSITE-ProRule annotation
Active sitei646 – 6461Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi530 – 5389ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase TTK (EC:2.7.12.1)
Alternative name(s):
ESK
PYT
Gene namesi
Name:Ttk
Synonyms:Esk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1194921. Ttk.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 856856Dual specificity protein kinase TTKPRO_0000086775Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei32 – 321PhosphothreonineBy similarity
Modified residuei36 – 361PhosphoserineBy similarity
Modified residuei277 – 2771PhosphoserineBy similarity
Modified residuei342 – 3421PhosphoserineBy similarity
Modified residuei435 – 4351PhosphoserineBy similarity
Modified residuei454 – 4541PhosphoserineBy similarity
Modified residuei820 – 8201PhosphoserineBy similarity

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP35761.
PaxDbiP35761.
PeptideAtlasiP35761.
PRIDEiP35761.

PTM databases

iPTMnetiP35761.
PhosphoSiteiP35761.

Expressioni

Tissue specificityi

Present in rapidly proliferating cell lines; high levels in testis, bone marrow, spleen and thymus. Low levels in brain, heart, lung and kidney.

Gene expression databases

BgeeiENSMUSG00000038379.
CleanExiMM_TTK.
ExpressionAtlasiP35761. baseline and differential.
GenevisibleiP35761. MM.

Interactioni

Subunit structurei

Interacts with TPR; the interactions occurs in a microtubule-independent manner.By similarity

Protein-protein interaction databases

IntActiP35761. 4 interactions.
STRINGi10090.ENSMUSP00000064839.

Structurei

3D structure databases

ProteinModelPortaliP35761.
SMRiP35761. Positions 55-191, 460-837.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini524 – 790267Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0596. Eukaryota.
ENOG410XRRQ. LUCA.
GeneTreeiENSGT00790000123008.
HOGENOMiHOG000013174.
HOVERGENiHBG001029.
InParanoidiP35761.
OMAiMSCFRTP.
OrthoDBiEOG091G0GP7.
PhylomeDBiP35761.
TreeFamiTF105420.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR027084. Dual_sp_prot_kinse_Ttk.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR22974:SF21. PTHR22974:SF21. 2 hits.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P35761-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAEELIGSS VTIDSIMSKM RDIKNKINED CTDELSLSKI CADHTETVNQ
60 70 80 90 100
IMRVGNTPEN WLNFLLKLEK NSSPLNDDLL NKLIGRYSQA IEVLPPDKYG
110 120 130 140 150
QNESFARIQV RLAELKAIQE PDDARDYFQM ARENCKKFAF VHVSFAQFEL
160 170 180 190 200
SQGNLKKSEQ LLHKAVETGA VPLQMLETAM RNLHLQKKQL LPEEDKKSVS
210 220 230 240 250
ASTVLSAQEP FSSSLGNVQN RSISCESRGQ AGAARVLYGE NLPPQDAEVR
260 270 280 290 300
HQNPFKQTHA AKRSCPFGRV PVNLLNSPDF YVKTDSSAVT QLTTRLALSS
310 320 330 340 350
VPLPYVTCLL HLQLLALAGL AKGSGPDRDA ILPGSRPRGS DSYELRGLKP
360 370 380 390 400
IQTIYLKDSL VSNEKSSELM SDLIALKSKT DSSLTKLEET KPEIAERRPM
410 420 430 440 450
QWQSTRKPEC VFQNPAAFAP LRHVPDVTPK ADKESPPISV PKWLDPKSAC
460 470 480 490 500
ETPSSSSLDD YMKCFKTPVV KNDFPPACPS STPYSQLARL QQQQQQGLST
510 520 530 540 550
PLQSLQISGS SSINECISVN GRIYSILKQI GSGGSSKVFQ VLNEKKQINA
560 570 580 590 600
IKYVNLEDAD SQTIESYRNE IAFLNKLQQH SDKIIRLYDY EITEQYIYMV
610 620 630 640 650
MECGNIDLNS WLKKKKSINP WERKSYWKNM LEAVHIIHQH GIVHSDLKPA
660 670 680 690 700
NFVIVDGMLK LIDFGIANQM QPDTTSIVKD SQVGTVNYMA PEAIRDMSSS
710 720 730 740 750
RENSKIRTKV SPRSDVWSLG CILYYMTYGR TPFQHIINQV SKLHAIINPA
760 770 780 790 800
HEIEFPEISE KDLRDVLKCC LVRNPKERIS IPELLTHPYV QIQPHPGSQM
810 820 830 840 850
ARGATDEMKY VLGQLVGLNS PNSILKTAKT LYERYNCGEG QDSSSSKTFD

KKRERK
Length:856
Mass (Da):96,211
Last modified:June 1, 1994 - v1
Checksum:i31F8C16C195E6E86
GO
Isoform 2 (identifier: P35761-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     296-321: Missing.

Show »
Length:830
Mass (Da):93,539
Checksum:i597F84421324EB7A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei296 – 32126Missing in isoform 2. 1 PublicationVSP_004875Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86377 mRNA. Translation: AAA37578.1.
CCDSiCCDS52874.1. [P35761-2]
PIRiA44439.
B44439.
UniGeneiMm.1904.

Genome annotation databases

EnsembliENSMUST00000070326; ENSMUSP00000064839; ENSMUSG00000038379. [P35761-2]
UCSCiuc009qwo.2. mouse. [P35761-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86377 mRNA. Translation: AAA37578.1.
CCDSiCCDS52874.1. [P35761-2]
PIRiA44439.
B44439.
UniGeneiMm.1904.

3D structure databases

ProteinModelPortaliP35761.
SMRiP35761. Positions 55-191, 460-837.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP35761. 4 interactions.
STRINGi10090.ENSMUSP00000064839.

PTM databases

iPTMnetiP35761.
PhosphoSiteiP35761.

Proteomic databases

MaxQBiP35761.
PaxDbiP35761.
PeptideAtlasiP35761.
PRIDEiP35761.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070326; ENSMUSP00000064839; ENSMUSG00000038379. [P35761-2]
UCSCiuc009qwo.2. mouse. [P35761-2]

Organism-specific databases

MGIiMGI:1194921. Ttk.

Phylogenomic databases

eggNOGiKOG0596. Eukaryota.
ENOG410XRRQ. LUCA.
GeneTreeiENSGT00790000123008.
HOGENOMiHOG000013174.
HOVERGENiHBG001029.
InParanoidiP35761.
OMAiMSCFRTP.
OrthoDBiEOG091G0GP7.
PhylomeDBiP35761.
TreeFamiTF105420.

Enzyme and pathway databases

BRENDAi2.7.12.1. 3474.

Miscellaneous databases

PROiP35761.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038379.
CleanExiMM_TTK.
ExpressionAtlasiP35761. baseline and differential.
GenevisibleiP35761. MM.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR027084. Dual_sp_prot_kinse_Ttk.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR22974:SF21. PTHR22974:SF21. 2 hits.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTTK_MOUSE
AccessioniPrimary (citable) accession number: P35761
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.