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P35754 (GLRX1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutaredoxin-1
Alternative name(s):
Thioltransferase-1
Short name=TTase-1
Gene names
Name:GLRX
Synonyms:GRX
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length106 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glutaredoxin family.

Contains 1 glutaredoxin domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.13 Ref.14
Chain2 – 106105Glutaredoxin-1
PRO_0000141600

Regions

Domain3 – 106104Glutaredoxin

Amino acid modifications

Modified residue21N-acetylalanine Ref.14
Modified residue91N6-succinyllysine By similarity
Disulfide bond23 ↔ 26Redox-active
Disulfide bond79 ↔ 83

Natural variations

Natural variant471D → Y.
Corresponds to variant rs4767 [ dbSNP | Ensembl ].
VAR_049189

Experimental info

Sequence conflict961L → V in BAA04769. Ref.1

Secondary structure

.................... 106
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P35754 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: BB86FED55967EBE2

FASTA10611,776
        10         20         30         40         50         60 
MAQEFVNCKI QPGKVVVFIK PTCPYCRRAQ EILSQLPIKQ GLLEFVDITA TNHTNEIQDY 

        70         80         90        100 
LQQLTGARTV PRVFIGKDCI GGCSDLVSLQ QSGELLTRLK QIGALQ 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of the cDNA encoding human glutaredoxin."
Fernando M.R., Sumimoto H., Nanri H., Kawabata S., Iwanaga S., Minakami S., Fukumaki Y., Takeshige K.
Biochim. Biophys. Acta 1218:229-231(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Purification from placenta, amino acid sequence, structure comparisons and cDNA cloning of human glutaredoxin."
Padilla C.A., Martinez-Galisteo E., Barcena J.A., Spyrou G., Holmgren A.
Eur. J. Biochem. 227:27-34(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Spleen.
[3]"Purification, cloning and expression of dehydroascorbic acid-reducing activity from human neutrophils: identification as glutaredoxin."
Park J.B., Levine M.
Biochem. J. 315:931-938(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[4]"The human glutaredoxin gene: determination of its organization, transcription start point, and promoter analysis."
Park J.B., Levine M.
Gene 197:189-193(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Human lens thioltransferase: cloning, purification, and function."
Qiao F., Xing K.Y., Liu A., Ehlers N., Raghavachari N., Lou M.F.
Invest. Ophthalmol. Vis. Sci. 42:743-751(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lens.
[6]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[10]NIEHS SNPs program
Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[11]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[12]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver, Skin and Urinary bladder.
[13]"The primary structure and properties of thioltransferase (glutaredoxin) from human red blood cells."
Papov V.V., Gravina S.A., Mieyal J.J., Biemann K.
Protein Sci. 3:428-434(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-106.
Tissue: Blood.
[14]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[15]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"The NMR solution structure of human glutaredoxin in the fully reduced form."
Sun C., Berardi M.J., Bushweller J.H.
J. Mol. Biol. 280:687-701(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
[17]"Reactivity of the human thioltransferase (glutaredoxin) C7S, C25S, C78S, C82S mutant and NMR solution structure of its glutathionyl mixed disulfide intermediate reflect catalytic specificity."
Yang Y., Jao S.C., Nanduri S., Starke D.W., Mieyal J.J., Qin J.
Biochemistry 37:17145-17156(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D21238 mRNA. Translation: BAA04769.1.
X76648 mRNA. Translation: CAA54094.1.
AF069668 mRNA. Translation: AAC35798.1.
AF115105, AF115104 Genomic DNA. Translation: AAC98318.1.
AF162769 mRNA. Translation: AAD43353.1.
BT006689 mRNA. Translation: AAP35335.1.
CR450312 mRNA. Translation: CAG29308.1.
CR542165 mRNA. Translation: CAG46962.1.
AK311868 mRNA. Translation: BAG34809.1.
DQ026062 Genomic DNA. Translation: AAY29058.1.
CH471084 Genomic DNA. Translation: EAW96056.1.
BC005304 mRNA. Translation: AAH05304.1.
BC010965 mRNA. Translation: AAH10965.1.
BC106075 mRNA. Translation: AAI06076.1.
CCDSCCDS4078.1.
PIRS47472. S68701.
RefSeqNP_001112362.1. NM_001118890.1.
NP_001230587.1. NM_001243658.1.
NP_001230588.1. NM_001243659.1.
NP_002055.1. NM_002064.2.
UniGeneHs.28988.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B4QNMR-A2-106[»]
1JHBNMR-A1-106[»]
ProteinModelPortalP35754.
SMRP35754. Positions 2-106.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109007. 8 interactions.
IntActP35754. 4 interactions.
MINTMINT-4649604.
STRING9606.ENSP00000237858.

Chemistry

DrugBankDB00143. Glutathione.

PTM databases

PhosphoSiteP35754.

Polymorphism databases

DMDM1346143.

2D gel databases

UCD-2DPAGEP35754.

Proteomic databases

MaxQBP35754.
PaxDbP35754.
PeptideAtlasP35754.
PRIDEP35754.

Protocols and materials databases

DNASU2745.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000237858; ENSP00000237858; ENSG00000173221.
ENST00000379979; ENSP00000369314; ENSG00000173221.
ENST00000505427; ENSP00000427353; ENSG00000173221.
ENST00000508780; ENSP00000422708; ENSG00000173221.
ENST00000512469; ENSP00000424636; ENSG00000173221.
GeneID2745.
KEGGhsa:2745.
UCSCuc003kln.4. human.

Organism-specific databases

CTD2745.
GeneCardsGC05M095087.
HGNCHGNC:4330. GLRX.
HPACAB008634.
MIM600443. gene.
neXtProtNX_P35754.
PharmGKBPA28731.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0695.
HOGENOMHOG000095204.
HOVERGENHBG000283.
InParanoidP35754.
KOK03676.
OMADAKYLTI.
OrthoDBEOG7QRQWZ.
PhylomeDBP35754.
TreeFamTF326994.

Enzyme and pathway databases

BioCycMetaCyc:HS04268-MONOMER.
ReactomeREACT_111217. Metabolism.
SABIO-RKP35754.

Gene expression databases

BgeeP35754.
CleanExHS_GLRX.
GenevestigatorP35754.

Family and domain databases

Gene3D3.40.30.10. 1 hit.
InterProIPR011767. GLR_AS.
IPR002109. Glutaredoxin.
IPR011899. Glutaredoxin_euk/vir.
IPR014025. Glutaredoxin_subgr.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF00462. Glutaredoxin. 1 hit.
[Graphical view]
PRINTSPR00160. GLUTAREDOXIN.
SUPFAMSSF52833. SSF52833. 1 hit.
TIGRFAMsTIGR02180. GRX_euk. 1 hit.
PROSITEPS00195. GLUTAREDOXIN_1. 1 hit.
PS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP35754.
GeneWikiGLRX.
GenomeRNAi2745.
NextBio10820.
PROP35754.
SOURCESearch...

Entry information

Entry nameGLRX1_HUMAN
AccessionPrimary (citable) accession number: P35754
Secondary accession number(s): B2R4L2, Q3KQS1, Q6ICT1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM