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P35751

- ANP1_RHIDE

UniProt

P35751 - ANP1_RHIDE

Protein

Ice-structuring protein RD1

Gene
N/A
Organism
Rhigophila dearborni (Antarctic eelpout) (Lycodichthys dearborni)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei9 – 91Important for ice-bindingBy similarity
    Sitei14 – 141Important for ice-bindingBy similarity
    Sitei18 – 181Important for ice-bindingBy similarity
    Sitei44 – 441Important for ice-bindingBy similarity

    GO - Biological processi

    1. response to freezing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antifreeze protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ice-structuring protein RD1
    Short name:
    ISP RD1
    Alternative name(s):
    Antifreeze peptide RD1
    OrganismiRhigophila dearborni (Antarctic eelpout) (Lycodichthys dearborni)
    Taxonomic identifieri8201 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataPercomorphariaPerciformesCottioideiZoarcalesZoarcidaeLycodinaeLycodichthys

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6464Ice-structuring protein RD1PRO_0000155151Add
    BLAST

    Expressioni

    Tissue specificityi

    Detected in blood serum (at protein level).1 Publication

    Structurei

    Secondary structure

    1
    64
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 96
    Helixi19 – 213
    Beta strandi22 – 254
    Helixi34 – 363
    Helixi37 – 404
    Beta strandi44 – 474
    Helixi57 – 593

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1UCSX-ray0.62A1-64[»]
    ProteinModelPortaliP35751.
    SMRiP35751. Positions 1-64.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP35751.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 6360AFP-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the type-III AFP family.Curated
    Contains 1 AFP-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG004237.

    Family and domain databases

    Gene3Di3.90.1210.10. 1 hit.
    InterProiIPR006190. AFP_Neu5c_C.
    IPR006013. Antifreeze_III.
    IPR013974. SAF.
    [Graphical view]
    PfamiPF08666. SAF. 1 hit.
    [Graphical view]
    PRINTSiPR00357. ANTIFREEZIII.
    SUPFAMiSSF51269. SSF51269. 1 hit.
    PROSITEiPS50844. AFP_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35751-1 [UniParc]FASTAAdd to Basket

    « Hide

    NKASVVANQL IPINTALTLI MMKAEVVTPM GIPAEEIPKL VGMQVNRAVP   50
    LGTTLMPDMV KNYE 64
    Length:64
    Mass (Da):6,906
    Last modified:June 1, 1994 - v1
    Checksum:i154C6BE62D913192
    GO

    Sequence databases

    PIRiS53512.

    Cross-referencesi

    Sequence databases

    PIRi S53512.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1UCS X-ray 0.62 A 1-64 [» ]
    ProteinModelPortali P35751.
    SMRi P35751. Positions 1-64.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG004237.

    Miscellaneous databases

    EvolutionaryTracei P35751.

    Family and domain databases

    Gene3Di 3.90.1210.10. 1 hit.
    InterProi IPR006190. AFP_Neu5c_C.
    IPR006013. Antifreeze_III.
    IPR013974. SAF.
    [Graphical view ]
    Pfami PF08666. SAF. 1 hit.
    [Graphical view ]
    PRINTSi PR00357. ANTIFREEZIII.
    SUPFAMi SSF51269. SSF51269. 1 hit.
    PROSITEi PS50844. AFP_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Antifreeze peptide heterogeneity in an antarctic eel pout includes an unusually large major variant comprised of two 7 kDa type III AFPs linked in tandem."
      Wang X., Devries A.L., Cheng C.-H.C.
      Biochim. Biophys. Acta 1247:163-172(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    2. "The refined crystal structure of an eel pout type III antifreeze protein RD1 at 0.62-A resolution reveals structuRAl microheterogeneity of protein and solvation."
      Ko T.-P., Robinson H., Gao Y.-G., Cheng C.H.-C., DeVries A.L., Wang A.H.-J.
      Biophys. J. 84:1228-1237(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (0.62 ANGSTROMS).

    Entry informationi

    Entry nameiANP1_RHIDE
    AccessioniPrimary (citable) accession number: P35751
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3