Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P35751 (ANP1_RHIDE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ice-structuring protein RD1

Short name=ISP RD1
Alternative name(s):
Antifreeze peptide RD1
OrganismRhigophila dearborni (Antarctic eelpout) (Lycodichthys dearborni)
Taxonomic identifier8201 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataPercomorphariaPerciformesCottioideiZoarcalesZoarcidaeLycodinaeLycodichthys

Protein attributes

Sequence length64 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth By similarity.

Subcellular location

Secreted Ref.1.

Tissue specificity

Detected in blood serum (at protein level). Ref.1

Sequence similarities

Belongs to the type-III AFP family.

Contains 1 AFP-like domain.

Ontologies

Keywords
   Cellular componentSecreted
   Molecular functionAntifreeze protein
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processresponse to freezing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6464Ice-structuring protein RD1
PRO_0000155151

Regions

Domain4 – 6360AFP-like

Sites

Site91Important for ice-binding By similarity
Site141Important for ice-binding By similarity
Site181Important for ice-binding By similarity
Site441Important for ice-binding By similarity

Secondary structure

............. 64
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P35751 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 154C6BE62D913192

FASTA646,906
        10         20         30         40         50         60 
NKASVVANQL IPINTALTLI MMKAEVVTPM GIPAEEIPKL VGMQVNRAVP LGTTLMPDMV 


KNYE 

« Hide

References

[1]"Antifreeze peptide heterogeneity in an antarctic eel pout includes an unusually large major variant comprised of two 7 kDa type III AFPs linked in tandem."
Wang X., Devries A.L., Cheng C.-H.C.
Biochim. Biophys. Acta 1247:163-172(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"The refined crystal structure of an eel pout type III antifreeze protein RD1 at 0.62-A resolution reveals structuRAl microheterogeneity of protein and solvation."
Ko T.-P., Robinson H., Gao Y.-G., Cheng C.H.-C., DeVries A.L., Wang A.H.-J.
Biophys. J. 84:1228-1237(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (0.62 ANGSTROMS).

Cross-references

Sequence databases

PIRS53512.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UCSX-ray0.62A1-64[»]
ProteinModelPortalP35751.
SMRP35751. Positions 1-64.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG004237.

Family and domain databases

Gene3D3.90.1210.10. 1 hit.
InterProIPR006190. AFP_Neu5c_C.
IPR006013. Antifreeze_III.
IPR013974. SAF.
[Graphical view]
PfamPF08666. SAF. 1 hit.
[Graphical view]
PRINTSPR00357. ANTIFREEZIII.
SUPFAMSSF51269. SSF51269. 1 hit.
PROSITEPS50844. AFP_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP35751.

Entry information

Entry nameANP1_RHIDE
AccessionPrimary (citable) accession number: P35751
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references