Reviewed, UniProtKB/Swiss-Prot P35740 (HN_NDVC)

Names and origin

Protein names

Recommended name:
    Hemagglutinin-neuraminidase
    EC=3.2.1.18

Gene names

Name:

HN

Organism

Newcastle disease virus (strain Chi/85) (NDV)

Taxonomic identifier

11179 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Avulavirus

Virus host

Gallus gallus (Chicken) [TaxID: 9031]

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity. Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Ontologies

Keywords
Cellular component	Envelope protein Host cell membrane Host membrane Membrane Virion
Domain	Signal-anchor Transmembrane
Molecular function	Hemagglutinin Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell viral envelope Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: EC host cell surface receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Hemagglutinin-neuraminidase		PRO_0000142608
Regions
	Topological domain	1 – 26	26	Cytoplasmic Potential
	Transmembrane	27 – 47	21	Potential
	Topological domain	48 – 571	524	Extracellular Potential
Amino acid modifications
	Glycosylation	119	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	341	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	433	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	481	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	508	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	P35740-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 3110CD668D71EF76	FASTA	571	62,901
	10 20 30 40 50 60 MDRAVNRVVL ENEEREAKNT WRLVFRIAVL LLMVMTLAIS AAALVYSMGA STPRDLAGIS 70 80 90 100 110 120 TVISKTEDKV TSLLSSKQDV IDRIYKQVAL ESPLALLNTE SIIMNAITSL SYQINGAANN 130 140 150 160 170 180 SGCGEPVHDP DYIGGIGKEL IVDDISDVTS FYPSAYQEHL NFIPAPTTGS GCTRIPSFDM 190 200 210 220 230 240 STTHYCYTHN VILSGCRDHS HSHQYLALGV LRTSATGRVF FSTLRSINLD DTQNRKSCSV 250 260 270 280 290 300 SATPLGCDML CSKVTETEEE DYKSVTPTSM VHGRLGFDGQ YHEKDLDTTV LFKDWVANYP 310 320 330 340 350 360 GVGGGSFIDD RVWFPVYGGL KPNSPSDTAQ EGKYVIYKRY NNTCPDEQDY QIRMAKSSYK 370 380 390 400 410 420 PGRFGGKRVQ QAILSIKVST SLGEDPVLTI PPNTITLMGA EGRVLTVGTS HFLYQRGSSY 430 440 450 460 470 480 FSPALLYPMT VNNKTATLHS PYTFNAFTRP GSVPCQASAR CPNSCITGVY TDPYPLIFHR 490 500 510 520 530 540 NHTLRGVFGT MLDDEQARLN PVSAVFDNIS RSRVTRVSSS STKAAYTTST CFKVVKTNKA 550 560 570 YCLSIAEISN TLFGEFRIVP LLVEILKDDR V

References

[1]

"Newcastle disease virus evolution. I. Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene." Sakaguchi T., Toyoda T., Gotoh B., Inocencio N.M., Kuma K., Miyata T., Nagai Y. Virology 169:260-272(1989) [PubMed: 2705297] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M24716 Genomic RNA. Translation: AAA46666.1.
PIR	C36829.
3D structure databases
SMR	P35740. Positions 124-569.
ModBase	Search...
Protein family/group databases
CAZy	GH83. Glycoside Hydrolase Family 83.
Family and domain databases
InterPro	IPR000665. Hemagglutn-neuramid_glycoprot. IPR016285. Hemagglutn-neuramid_paramyxo. IPR011040. Neuraminidase. [Graphical view]
Pfam	PF00423. HN. 1 hit. [Graphical view]
PIRSF	PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HN_NDVC

Accession

Primary (citable) accession number: P35740

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 19, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)