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Protein

2-oxoisovalerate dehydrogenase subunit beta, mitochondrial

Gene

Bckdhb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

Catalytic activityi

3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2.

GO - Molecular functioni

GO - Biological processi

  • branched-chain amino acid catabolic process Source: HGNC
  • response to cAMP Source: RGD
  • response to glucocorticoid Source: RGD
  • response to nutrient Source: RGD

Keywordsi

Molecular functionOxidoreductase

Enzyme and pathway databases

BRENDAi1.2.4.4. 5301.
SABIO-RKiP35738.

Names & Taxonomyi

Protein namesi
Recommended name:
2-oxoisovalerate dehydrogenase subunit beta, mitochondrial (EC:1.2.4.4)
Alternative name(s):
Branched-chain alpha-keto acid dehydrogenase E1 component beta chain
Short name:
BCKDE1B
Short name:
BCKDH E1-beta
Gene namesi
Name:Bckdhb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2197. Bckdhb.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48MitochondrionSequence analysisAdd BLAST48
ChainiPRO_000002047149 – 3902-oxoisovalerate dehydrogenase subunit beta, mitochondrialAdd BLAST342

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei230N6-acetyllysineBy similarity1
Modified residuei239N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP35738.
PRIDEiP35738.

Interactioni

Subunit structurei

Heterotetramer of 2 alpha and 2 beta chains.By similarity

GO - Molecular functioni

  • protein complex binding Source: RGD

Protein-protein interaction databases

BioGridi248328. 1 interactor.
IntActiP35738. 1 interactor.
STRINGi10116.ENSRNOP00000013249.

Structurei

3D structure databases

ProteinModelPortaliP35738.
SMRiP35738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0525. Eukaryota.
COG0022. LUCA.
HOGENOMiHOG000281451.
HOVERGENiHBG108210.
InParanoidiP35738.
KOiK00167.
PhylomeDBiP35738.
TreeFamiTF105947.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
InterProiView protein in InterPro
IPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
PfamiView protein in Pfam
PF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
SMARTiView protein in SMART
SM00861. Transket_pyr. 1 hit.
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35738-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVAARAGG LLRLGAAGAE RRRRGLRCAA LVQGFLQPAV DDASQKRRVA
60 70 80 90 100
HFTFQPDPES LQYGQTQKMN LFQSITSALD NSLAKDPTAV IFGEDVAFGG
110 120 130 140 150
VFRCTVGLRD KYGKDRVFNT PLCEQGIVGF GIGIAVTGAT AIAEIQFADY
160 170 180 190 200
IFPAFDQIVN EAAKYRYRSG DLFNCGSLTI RAPWGCVGHG ALYHSQSPEA
210 220 230 240 250
FFAHCPGIKV VIPRSPFQAK GLLLSCIEDK NPCIFFEPKI LYRAAVEQVP
260 270 280 290 300
VEPYKIPLSQ AEVIQEGSDV TLVAWGTQVH VIREVASMAQ EKLGVSCEVI
310 320 330 340 350
DLRTIVPWDV DTVCKSVIKT GRLLISHEAP LTGGFASEIS STVQEECFLN
360 370 380 390
LEAPISRVCG YDTPFPHIFE PFYIPDKWKC YDALRKMINY
Length:390
Mass (Da):42,823
Last modified:December 4, 2007 - v3
Checksum:i7A562A868823A1BF
GO

Sequence cautioni

P35738: The sequence AAA73899 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated
P35738: The sequence AAA73899 differs from that shown. Reason: Frameshift at positions 101 and 120.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti255K → R in AAA73899 (PubMed:1390893).Curated1
Sequence conflicti303R → T in AAA73899 (PubMed:1390893).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03062593 Genomic DNA. No translation available.
AABR03062720 Genomic DNA. No translation available.
AABR03063125 Genomic DNA. No translation available.
AABR03063398 Genomic DNA. No translation available.
M94040 mRNA. Translation: AAA73899.1. Sequence problems.
PIRiS28950.
RefSeqiNP_062140.1. NM_019267.1.
UniGeneiRn.15623.

Genome annotation databases

GeneIDi29711.
KEGGirno:29711.
UCSCiRGD:2197. rat.

Similar proteinsi

Entry informationi

Entry nameiODBB_RAT
AccessioniPrimary (citable) accession number: P35738
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: December 4, 2007
Last modified: October 25, 2017
This is version 128 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome