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Protein

2-oxoisovalerate dehydrogenase subunit beta, mitochondrial

Gene

Bckdhb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

Catalytic activityi

3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2.

GO - Molecular functioni

GO - Biological processi

  • branched-chain amino acid catabolic process Source: HGNC
  • response to cAMP Source: RGD
  • response to glucocorticoid Source: RGD
  • response to nutrient Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

BRENDAi1.2.4.4. 5301.
SABIO-RKP35738.

Names & Taxonomyi

Protein namesi
Recommended name:
2-oxoisovalerate dehydrogenase subunit beta, mitochondrial (EC:1.2.4.4)
Alternative name(s):
Branched-chain alpha-keto acid dehydrogenase E1 component beta chain
Short name:
BCKDE1B
Short name:
BCKDH E1-beta
Gene namesi
Name:Bckdhb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2197. Bckdhb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848MitochondrionSequence analysisAdd
BLAST
Chaini49 – 3903422-oxoisovalerate dehydrogenase subunit beta, mitochondrialPRO_0000020471Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei230 – 2301N6-acetyllysineBy similarity
Modified residuei239 – 2391N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP35738.
PRIDEiP35738.

Expressioni

Gene expression databases

BgeeiENSRNOG00000009928.

Interactioni

Subunit structurei

Heterotetramer of 2 alpha and 2 beta chains.By similarity

GO - Molecular functioni

  • protein complex binding Source: RGD

Protein-protein interaction databases

BioGridi248328. 1 interaction.
STRINGi10116.ENSRNOP00000013249.

Structurei

3D structure databases

ProteinModelPortaliP35738.
SMRiP35738. Positions 65-390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0525. Eukaryota.
COG0022. LUCA.
HOGENOMiHOG000281451.
HOVERGENiHBG108210.
InParanoidiP35738.
KOiK00167.
PhylomeDBiP35738.
TreeFamiTF105947.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProiIPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
IPR033247. Transketolase_fam.
[Graphical view]
PANTHERiPTHR11624. PTHR11624. 1 hit.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35738-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVAARAGG LLRLGAAGAE RRRRGLRCAA LVQGFLQPAV DDASQKRRVA
60 70 80 90 100
HFTFQPDPES LQYGQTQKMN LFQSITSALD NSLAKDPTAV IFGEDVAFGG
110 120 130 140 150
VFRCTVGLRD KYGKDRVFNT PLCEQGIVGF GIGIAVTGAT AIAEIQFADY
160 170 180 190 200
IFPAFDQIVN EAAKYRYRSG DLFNCGSLTI RAPWGCVGHG ALYHSQSPEA
210 220 230 240 250
FFAHCPGIKV VIPRSPFQAK GLLLSCIEDK NPCIFFEPKI LYRAAVEQVP
260 270 280 290 300
VEPYKIPLSQ AEVIQEGSDV TLVAWGTQVH VIREVASMAQ EKLGVSCEVI
310 320 330 340 350
DLRTIVPWDV DTVCKSVIKT GRLLISHEAP LTGGFASEIS STVQEECFLN
360 370 380 390
LEAPISRVCG YDTPFPHIFE PFYIPDKWKC YDALRKMINY
Length:390
Mass (Da):42,823
Last modified:December 4, 2007 - v3
Checksum:i7A562A868823A1BF
GO

Sequence cautioni

The sequence AAA73899 differs from that shown.Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated
The sequence AAA73899 differs from that shown. Reason: Frameshift at positions 101 and 120. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti255 – 2551K → R in AAA73899 (PubMed:1390893).Curated
Sequence conflicti303 – 3031R → T in AAA73899 (PubMed:1390893).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03062593 Genomic DNA. No translation available.
AABR03062720 Genomic DNA. No translation available.
AABR03063125 Genomic DNA. No translation available.
AABR03063398 Genomic DNA. No translation available.
M94040 mRNA. Translation: AAA73899.1. Sequence problems.
PIRiS28950.
RefSeqiNP_062140.1. NM_019267.1.
UniGeneiRn.15623.

Genome annotation databases

GeneIDi29711.
KEGGirno:29711.
UCSCiRGD:2197. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03062593 Genomic DNA. No translation available.
AABR03062720 Genomic DNA. No translation available.
AABR03063125 Genomic DNA. No translation available.
AABR03063398 Genomic DNA. No translation available.
M94040 mRNA. Translation: AAA73899.1. Sequence problems.
PIRiS28950.
RefSeqiNP_062140.1. NM_019267.1.
UniGeneiRn.15623.

3D structure databases

ProteinModelPortaliP35738.
SMRiP35738. Positions 65-390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248328. 1 interaction.
STRINGi10116.ENSRNOP00000013249.

Proteomic databases

PaxDbiP35738.
PRIDEiP35738.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29711.
KEGGirno:29711.
UCSCiRGD:2197. rat.

Organism-specific databases

CTDi594.
RGDi2197. Bckdhb.

Phylogenomic databases

eggNOGiKOG0525. Eukaryota.
COG0022. LUCA.
HOGENOMiHOG000281451.
HOVERGENiHBG108210.
InParanoidiP35738.
KOiK00167.
PhylomeDBiP35738.
TreeFamiTF105947.

Enzyme and pathway databases

BRENDAi1.2.4.4. 5301.
SABIO-RKP35738.

Miscellaneous databases

PROiP35738.

Gene expression databases

BgeeiENSRNOG00000009928.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProiIPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
IPR033247. Transketolase_fam.
[Graphical view]
PANTHERiPTHR11624. PTHR11624. 1 hit.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiODBB_RAT
AccessioniPrimary (citable) accession number: P35738
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: December 4, 2007
Last modified: September 7, 2016
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.