Reviewed, UniProtKB/Swiss-Prot P35731 (FABG_YEAST)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] reductase
    EC=1.1.1.100
Alternative name(s):
    3-ketoacyl-acyl carrier protein reductase

Gene names

Name:	OAR1
Ordered Locus Names:	YKL055C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in biosynthesis of fatty acids in mitochondria By similarity.
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subcellular location	Mitochondrion. Ref.4
Miscellaneous	Present with 1760 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Mitochondrion
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aerobic respiration Ref.3 Inferred from mutant phenotype. Source: SGD fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Ref.3 Inferred from mutant phenotype. Source: SGD
Molecular function	3-oxoacyl-[acyl-carrier-protein] reductase activity Ref.3 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ECM4	P36156	1	EBI-26702,EBI-2042717

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	3-oxoacyl-[acyl-carrier-protein] reductase		PRO_0000054872
Regions
	Nucleotide binding	8 – 32	25	NAD or NADP By similarity
Sites
	Active site	198	1	Proton acceptor By similarity
	Binding site	183	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35731-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 62FC3BC347D3E933	FASTA	278	31,184
	10 20 30 40 50 60 MHYLPVAIVT GATRGIGKAI CQKLFQKGLS CIILGSTKES IERTAIDRGQ LQSGLSYQRQ 70 80 90 100 110 120 CAIAIDFKKW PHWLDYESYD GIEYFKDRPP LKQKYSTLFD PCNKWSNNER RYYVNLLINC 130 140 150 160 170 180 AGLTQESLSV RTTASQIQDI MNVNFMSPVT MTNICIKYMM KSQRRWPELS GQSARPTIVN 190 200 210 220 230 240 ISSILHSGKM KVPGTSVYSA SKAALSRFTE VLAAEMEPRN IRCFTISPGL VKGTDMIQNL 250 260 270 PVEAKEMLER TIGASGTSAP AEIAEEVWSL YSRTALET

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins." Rasmussen S.W. Yeast 10:S63-S68(1994) [PubMed: 8091862] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Two genes of the putative mitochondrial fatty acid synthase in the genome of Saccharomyces cerevisiae." Schneider R., Brors B., Buerger F., Camrath S., Weiss H. Curr. Genet. 32:384-388(1997) [PubMed: 9388293] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75781 Genomic DNA. Translation: CAA53417.1. Z28055 Genomic DNA. Translation: CAA81892.1.
PIR	S37877.
RefSeq	NP_012868.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5403N.
IntAct	P35731. 2 interactions.
STRING	P35731.
Genome annotation databases
Ensembl	YKL055C; YKL055C; YKL055C; Saccharomyces cerevisiae. [Genome view]
GeneID	853810.
GenomeReviews	Gene locus YKL055C in contig Y13137_GR.
KEGG	sce:YKL055C.
NMPDR	fig|4932.3.peg.3853.
Organism-specific databases
CYGD	YKL055c.
SGD	S000001538. OAR1.
Phylogenomic databases
HOGENOM	P35731.
OMA	EMLERTI.
Gene expression databases
ArrayExpress	P35731.
Genevestigator	P35731.
GermOnline	YKL055C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974975.

Entry information

Entry name

FABG_YEAST

Accession

Primary (citable) accession number: P35731

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 3, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)