P35731 (FABG_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase
EC=1.1.1.100

Alternative name(s):
3-ketoacyl-acyl carrier protein reductase

Gene names

Name:	OAR1
Ordered Locus Names:	YKL055C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in biosynthesis of fatty acids in mitochondria By similarity. Ref.4
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subcellular location	Mitochondrion Ref.5.
Miscellaneous	Present with 1760 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Cellular component	Mitochondrion
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aerobic respiration Inferred from mutant phenotype Ref.4. Source: SGD fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway fatty acid metabolic process Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	mitochondrion Inferred from mutant phenotype Ref.4. Source: SGD
Molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Inferred from direct assay PubMed 19571038. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	3-oxoacyl-[acyl-carrier-protein] reductase		PRO_0000054872
Regions
	Nucleotide binding	8 – 32	25	NAD or NADP By similarity
Sites
	Active site	198	1	Proton acceptor By similarity
	Binding site	183	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35731 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 62FC3BC347D3E933	FASTA	278	31,184
	10 20 30 40 50 60 MHYLPVAIVT GATRGIGKAI CQKLFQKGLS CIILGSTKES IERTAIDRGQ LQSGLSYQRQ 70 80 90 100 110 120 CAIAIDFKKW PHWLDYESYD GIEYFKDRPP LKQKYSTLFD PCNKWSNNER RYYVNLLINC 130 140 150 160 170 180 AGLTQESLSV RTTASQIQDI MNVNFMSPVT MTNICIKYMM KSQRRWPELS GQSARPTIVN 190 200 210 220 230 240 ISSILHSGKM KVPGTSVYSA SKAALSRFTE VLAAEMEPRN IRCFTISPGL VKGTDMIQNL 250 260 270 PVEAKEMLER TIGASGTSAP AEIAEEVWSL YSRTALET

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins." Rasmussen S.W. Yeast 10:S63-S68(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Two genes of the putative mitochondrial fatty acid synthase in the genome of Saccharomyces cerevisiae." Schneider R., Brors B., Buerger F., Camrath S., Weiss H. Curr. Genet. 32:384-388(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75781 Genomic DNA. Translation: CAA53417.1. Z28055 Genomic DNA. Translation: CAA81892.1. BK006944 Genomic DNA. Translation: DAA09102.1.
PIR	S37877.
RefSeq	NP_012868.1. NM_001179621.2.
3D structure databases
ProteinModelPortal	P35731.
SMR	P35731. Positions 6-275.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5403N.
MINT	MINT-478684.
STRING	4932.YKL055C.
Proteomic databases
PaxDb	P35731.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL055C; YKL055C; YKL055C.
GeneID	853810.
KEGG	sce:YKL055C.
Organism-specific databases
CYGD	YKL055c.
SGD	S000001538. OAR1.
Phylogenomic databases
eggNOG	COG1028.
KO	K00540.
OMA	DIMNVNF.
OrthoDB	EOG48H0CZ.
Enzyme and pathway databases
UniPathway	UPA00094.
Gene expression databases
Genevestigator	P35731.
GermOnline	YKL055C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974975.

Entry information

Entry name

FABG_YEAST

Accession

Primary (citable) accession number: P35731
Secondary accession number(s): D6VXN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program