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Protein

Nucleoporin NUP120

Gene

NUP120

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP120 is involved in nuclear poly(A)+ RNA and pre-ribosome export, in GSP1 nuclear import, in NPC assembly and distribution, as well as in nuclear envelope organization.5 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • nucleocytoplasmic transporter activity Source: GO_Central
  • structural constituent of nuclear pore Source: SGD

GO - Biological processi

  • double-strand break repair Source: SGD
  • maintenance of chromatin silencing at telomere Source: SGD
  • mRNA export from nucleus Source: SGD
  • mRNA export from nucleus in response to heat stress Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • nuclear pore distribution Source: SGD
  • positive regulation of transcription, DNA-templated Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
  • protein export from nucleus Source: SGD
  • protein import into nucleus Source: SGD
  • ribosomal large subunit export from nucleus Source: SGD
  • telomere tethering at nuclear periphery Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31856-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP120
Alternative name(s):
Nuclear pore protein NUP120
Gene namesi
Name:NUP120
Synonyms:RAT2
Ordered Locus Names:YKL057C
ORF Names:YKL313, YKL314
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL057C.
SGDiS000001540. NUP120.

Subcellular locationi

GO - Cellular componenti

  • chromosome, telomeric region Source: GOC
  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: SGD
  • nuclear pore outer ring Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048361 – 1037Nucleoporin NUP120Add BLAST1037

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei417PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35729.
PRIDEiP35729.

PTM databases

iPTMnetiP35729.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NUP120 is part of the heptameric 0.5 MDa autoassembling NUP84 NPC subcomplex (NUP84, NUP85, NUP120, NUP133, NUP145C, SEC13 and SEH1).

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-11713,EBI-11713
NUP145P4968714EBI-11713,EBI-11730
NUP84P528916EBI-11713,EBI-12337
NUP85P4667310EBI-11713,EBI-12345

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi34076. 207 interactors.
DIPiDIP-2721N.
IntActiP35729. 20 interactors.
MINTiMINT-491139.

Structurei

Secondary structure

11037
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 10Combined sources9
Turni11 – 14Combined sources4
Beta strandi16 – 19Combined sources4
Beta strandi23 – 26Combined sources4
Beta strandi55 – 61Combined sources7
Beta strandi63 – 65Combined sources3
Beta strandi67 – 72Combined sources6
Beta strandi78 – 83Combined sources6
Helixi84 – 86Combined sources3
Turni87 – 89Combined sources3
Beta strandi92 – 96Combined sources5
Beta strandi101 – 103Combined sources3
Helixi104 – 107Combined sources4
Beta strandi108 – 112Combined sources5
Beta strandi114 – 126Combined sources13
Beta strandi128 – 134Combined sources7
Helixi135 – 139Combined sources5
Turni158 – 160Combined sources3
Beta strandi163 – 168Combined sources6
Beta strandi170 – 178Combined sources9
Beta strandi179 – 181Combined sources3
Beta strandi183 – 186Combined sources4
Beta strandi187 – 192Combined sources6
Helixi203 – 208Combined sources6
Beta strandi216 – 219Combined sources4
Beta strandi223 – 229Combined sources7
Turni230 – 232Combined sources3
Beta strandi233 – 238Combined sources6
Beta strandi242 – 247Combined sources6
Turni248 – 251Combined sources4
Beta strandi252 – 258Combined sources7
Turni259 – 262Combined sources4
Beta strandi280 – 291Combined sources12
Beta strandi293 – 295Combined sources3
Beta strandi297 – 304Combined sources8
Beta strandi307 – 309Combined sources3
Beta strandi312 – 314Combined sources3
Beta strandi318 – 320Combined sources3
Turni325 – 327Combined sources3
Beta strandi330 – 338Combined sources9
Beta strandi343 – 345Combined sources3
Beta strandi348 – 357Combined sources10
Beta strandi360 – 368Combined sources9
Beta strandi370 – 372Combined sources3
Beta strandi375 – 381Combined sources7
Beta strandi383 – 385Combined sources3
Helixi386 – 392Combined sources7
Helixi406 – 415Combined sources10
Helixi417 – 428Combined sources12
Turni429 – 431Combined sources3
Helixi440 – 457Combined sources18
Beta strandi460 – 466Combined sources7
Turni467 – 469Combined sources3
Beta strandi470 – 477Combined sources8
Beta strandi480 – 486Combined sources7
Helixi489 – 494Combined sources6
Turni495 – 499Combined sources5
Helixi505 – 517Combined sources13
Helixi522 – 537Combined sources16
Beta strandi538 – 540Combined sources3
Beta strandi542 – 544Combined sources3
Helixi546 – 557Combined sources12
Turni558 – 560Combined sources3
Helixi564 – 574Combined sources11
Helixi579 – 588Combined sources10
Turni589 – 591Combined sources3
Helixi609 – 639Combined sources31
Turni644 – 647Combined sources4
Helixi648 – 670Combined sources23
Helixi672 – 680Combined sources9
Beta strandi681 – 684Combined sources4
Beta strandi685 – 688Combined sources4
Helixi695 – 711Combined sources17
Helixi719 – 728Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3F7FX-ray2.60A/B/C/D1-729[»]
3H7NX-ray3.00A/B/C/D1-729[»]
3HXRX-ray3.00A1-757[»]
4XMMX-ray7.38E2-1037[»]
4XMNX-ray7.60E1-1037[»]
ProteinModelPortaliP35729.
SMRiP35729.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35729.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 152Leucine-zipper 1Sequence analysisAdd BLAST22
Regioni290 – 311Leucine-zipper 2Sequence analysisAdd BLAST22

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000113870.
InParanoidiP35729.
KOiK14303.
OMAiPYDFTVR.
OrthoDBiEOG092C091L.

Sequencei

Sequence statusi: Complete.

P35729-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACLSRIDAN LLQYYEKPEP NNTVDLYVSN NSNNNGLKEG DKSISTPVPQ
60 70 80 90 100
PYGSEYSNCL LLSNSEYICY HFSSRSTLLT FYPLSDAYHG KTINIHLPNA
110 120 130 140 150
SMNQRYTLTI QEVEQQLLVN VILKDGSFLT LQLPLSFLFS SANTLNGEWF
160 170 180 190 200
HLQNPYDFTV RVPHFLFYVS PQFSVVFLED GGLLGLKKVD GVHYEPLLFN
210 220 230 240 250
DNSYLKSLTR FFSRSSKSDY DSVISCKLFH ERYLIVLTQN CHLKIWDLTS
260 270 280 290 300
FTLIQDYDMV SQSDSDPSHF RKVEAVGEYL SLYNNTLVTL LPLENGLFQM
310 320 330 340 350
GTLLVDSSGI LTYTFQNNIP TNLSASAIWS IVDLVLTRPL ELNVEASYLN
360 370 380 390 400
LIVLWKSGTA SKLQILNVND ESFKNYEWIE SVNKSLVDLQ SEHDLDIVTK
410 420 430 440 450
TGDVERGFCN LKSRYGTQIF ERAQQILSEN KIIMAHNEDE EYLANLETIL
460 470 480 490 500
RDVKTAFNEA SSITLYGDEI ILVNCFQPYN HSLYKLNTTV ENWFYNMHSE
510 520 530 540 550
TDGSELFKYL RTLNGFASTL SNDVLRSISK KFLDIITGEL PDSMTTVEKF
560 570 580 590 600
TDIFKNCLEN QFEITNLKIL FDELNSFDIP VVLNDLINNQ MKPGIFWKKD
610 620 630 640 650
FISAIKFDGF TSIISLESLH QLLSIHYRIT LQVLLTFVLF DLDTEIFGQH
660 670 680 690 700
ISTLLDLHYK QFLLLNLYRQ DKCLLAEVLL KDSSEFSFGV KFFNYGQLIA
710 720 730 740 750
YIDSLNSNVY NASITENSFF MTFFRSYIIE NTSHKNIRFF LENVECPFYL
760 770 780 790 800
RHNEVQEFMF AMTLFSCGNF DQSYEIFQLH DYPEAINDKL PTFLEDLKSE
810 820 830 840 850
NYHGDSIWKD LLCTFTVPYR HSAFYYQLSL LFDRNNSQEF ALKCISKSAE
860 870 880 890 900
YSLKEIQIEE LQDFKEKQHI HYLNLLIHFR MFEEVLDVLR LGHECLSDTV
910 920 930 940 950
RTNFLQLLLQ EDIYSRDFFS TLLRLCNAHS DNGELYLRTV DIKIVDSILS
960 970 980 990 1000
QNLRSGDWEC FKKLYCFRML NKSERAAAEV LYQYILMQAD LDVIRKRKCY
1010 1020 1030
LMVINVLSSF DSAYDQWILN GSKVVTLTDL RDELRGL
Length:1,037
Mass (Da):120,448
Last modified:June 1, 1994 - v1
Checksum:iD4655E6116C54503
GO

Sequence cautioni

The sequence CAA53415 differs from that shown. Reason: Frameshift at position 442.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75781 Genomic DNA. Translation: CAA53414.1. Frameshift.
X75781 Genomic DNA. Translation: CAA53415.1. Frameshift.
Z28057 Genomic DNA. Translation: CAA81894.1.
BK006944 Genomic DNA. Translation: DAA09100.1.
PIRiS37879.
RefSeqiNP_012866.1. NM_001179623.1.

Genome annotation databases

EnsemblFungiiYKL057C; YKL057C; YKL057C.
GeneIDi853808.
KEGGisce:YKL057C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75781 Genomic DNA. Translation: CAA53414.1. Frameshift.
X75781 Genomic DNA. Translation: CAA53415.1. Frameshift.
Z28057 Genomic DNA. Translation: CAA81894.1.
BK006944 Genomic DNA. Translation: DAA09100.1.
PIRiS37879.
RefSeqiNP_012866.1. NM_001179623.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3F7FX-ray2.60A/B/C/D1-729[»]
3H7NX-ray3.00A/B/C/D1-729[»]
3HXRX-ray3.00A1-757[»]
4XMMX-ray7.38E2-1037[»]
4XMNX-ray7.60E1-1037[»]
ProteinModelPortaliP35729.
SMRiP35729.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34076. 207 interactors.
DIPiDIP-2721N.
IntActiP35729. 20 interactors.
MINTiMINT-491139.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiP35729.

Proteomic databases

MaxQBiP35729.
PRIDEiP35729.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL057C; YKL057C; YKL057C.
GeneIDi853808.
KEGGisce:YKL057C.

Organism-specific databases

EuPathDBiFungiDB:YKL057C.
SGDiS000001540. NUP120.

Phylogenomic databases

HOGENOMiHOG000113870.
InParanoidiP35729.
KOiK14303.
OMAiPYDFTVR.
OrthoDBiEOG092C091L.

Enzyme and pathway databases

BioCyciYEAST:G3O-31856-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP35729.
PROiP35729.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiNU120_YEAST
AccessioniPrimary (citable) accession number: P35729
Secondary accession number(s): D6VXN0, P35730
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.