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P35729

- NU120_YEAST

UniProt

P35729 - NU120_YEAST

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Protein

Nucleoporin NUP120

Gene

NUP120

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP120 is involved in nuclear poly(A)+ RNA and pre-ribosome export, in GSP1 nuclear import, in NPC assembly and distribution, as well as in nuclear envelope organization.5 Publications

GO - Molecular functioni

  1. structural constituent of nuclear pore Source: SGD

GO - Biological processi

  1. double-strand break repair Source: SGD
  2. maintenance of chromatin silencing at telomere Source: SGD
  3. mRNA export from nucleus Source: SGD
  4. mRNA export from nucleus in response to heat stress Source: SGD
  5. negative regulation of transcription from RNA polymerase II promoter Source: SGD
  6. nuclear pore distribution Source: SGD
  7. positive regulation of transcription, DNA-templated Source: SGD
  8. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  9. posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
  10. protein export from nucleus Source: SGD
  11. protein import into nucleus Source: SGD
  12. ribosomal large subunit export from nucleus Source: SGD
  13. telomere tethering at nuclear periphery Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31856-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP120
Alternative name(s):
Nuclear pore protein NUP120
Gene namesi
Name:NUP120
Synonyms:RAT2
Ordered Locus Names:YKL057C
ORF Names:YKL313, YKL314
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL057c.
SGDiS000001540. NUP120.

Subcellular locationi

GO - Cellular componenti

  1. nuclear pore Source: SGD
  2. nuclear pore outer ring Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10371037Nucleoporin NUP120PRO_0000204836Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei417 – 4171Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35729.
PaxDbiP35729.
PeptideAtlasiP35729.

Expressioni

Gene expression databases

GenevestigatoriP35729.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NUP120 is part of the heptameric 0.5 MDa autoassembling NUP84 NPC subcomplex (NUP84, NUP85, NUP120, NUP133, NUP145C, SEC13 and SEH1).

Binary interactionsi

WithEntry#Exp.IntActNotes
NUP145P496873EBI-11713,EBI-11730
NUP85P466733EBI-11713,EBI-12345

Protein-protein interaction databases

BioGridi34076. 204 interactions.
DIPiDIP-2721N.
IntActiP35729. 17 interactions.
MINTiMINT-491139.
STRINGi4932.YKL057C.

Structurei

Secondary structure

1
1037
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 109
Turni11 – 144
Beta strandi16 – 194
Beta strandi23 – 264
Beta strandi55 – 617
Beta strandi63 – 653
Beta strandi67 – 726
Beta strandi78 – 836
Helixi84 – 863
Turni87 – 893
Beta strandi92 – 965
Beta strandi101 – 1033
Helixi104 – 1074
Beta strandi108 – 1125
Beta strandi114 – 12613
Beta strandi128 – 1347
Helixi135 – 1395
Turni158 – 1603
Beta strandi163 – 1686
Beta strandi170 – 1789
Beta strandi179 – 1813
Beta strandi183 – 1864
Beta strandi187 – 1926
Helixi203 – 2086
Beta strandi216 – 2194
Beta strandi223 – 2297
Turni230 – 2323
Beta strandi233 – 2386
Beta strandi242 – 2476
Turni248 – 2514
Beta strandi252 – 2587
Turni259 – 2624
Beta strandi280 – 29112
Beta strandi293 – 2953
Beta strandi297 – 3048
Beta strandi307 – 3093
Beta strandi312 – 3143
Beta strandi318 – 3203
Turni325 – 3273
Beta strandi330 – 3389
Beta strandi343 – 3453
Beta strandi348 – 35710
Beta strandi360 – 3689
Beta strandi370 – 3723
Beta strandi375 – 3817
Beta strandi383 – 3853
Helixi386 – 3927
Helixi406 – 41510
Helixi417 – 42812
Turni429 – 4313
Helixi440 – 45718
Beta strandi460 – 4667
Turni467 – 4693
Beta strandi470 – 4778
Beta strandi480 – 4867
Helixi489 – 4946
Turni495 – 4995
Helixi505 – 51713
Helixi522 – 53716
Beta strandi538 – 5403
Beta strandi542 – 5443
Helixi546 – 55712
Turni558 – 5603
Helixi564 – 57411
Helixi579 – 58810
Turni589 – 5913
Helixi609 – 63931
Turni644 – 6474
Helixi648 – 67023
Helixi672 – 6809
Beta strandi681 – 6844
Beta strandi685 – 6884
Helixi695 – 71117
Helixi719 – 72810

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3F7FX-ray2.60A/B/C/D1-729[»]
3H7NX-ray3.00A/B/C/D1-729[»]
3HXRX-ray3.00A1-757[»]
ProteinModelPortaliP35729.
SMRiP35729. Positions 1-729.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35729.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni131 – 15222Leucine-zipper 1Sequence AnalysisAdd
BLAST
Regioni290 – 31122Leucine-zipper 2Sequence AnalysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG19505.
HOGENOMiHOG000113870.
InParanoidiP35729.
OMAiCHLKIWD.
OrthoDBiEOG7MKWFP.

Family and domain databases

InterProiIPR021717. Nucleoporin_Nup160.
[Graphical view]
PfamiPF11715. Nup160. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35729-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MACLSRIDAN LLQYYEKPEP NNTVDLYVSN NSNNNGLKEG DKSISTPVPQ
60 70 80 90 100
PYGSEYSNCL LLSNSEYICY HFSSRSTLLT FYPLSDAYHG KTINIHLPNA
110 120 130 140 150
SMNQRYTLTI QEVEQQLLVN VILKDGSFLT LQLPLSFLFS SANTLNGEWF
160 170 180 190 200
HLQNPYDFTV RVPHFLFYVS PQFSVVFLED GGLLGLKKVD GVHYEPLLFN
210 220 230 240 250
DNSYLKSLTR FFSRSSKSDY DSVISCKLFH ERYLIVLTQN CHLKIWDLTS
260 270 280 290 300
FTLIQDYDMV SQSDSDPSHF RKVEAVGEYL SLYNNTLVTL LPLENGLFQM
310 320 330 340 350
GTLLVDSSGI LTYTFQNNIP TNLSASAIWS IVDLVLTRPL ELNVEASYLN
360 370 380 390 400
LIVLWKSGTA SKLQILNVND ESFKNYEWIE SVNKSLVDLQ SEHDLDIVTK
410 420 430 440 450
TGDVERGFCN LKSRYGTQIF ERAQQILSEN KIIMAHNEDE EYLANLETIL
460 470 480 490 500
RDVKTAFNEA SSITLYGDEI ILVNCFQPYN HSLYKLNTTV ENWFYNMHSE
510 520 530 540 550
TDGSELFKYL RTLNGFASTL SNDVLRSISK KFLDIITGEL PDSMTTVEKF
560 570 580 590 600
TDIFKNCLEN QFEITNLKIL FDELNSFDIP VVLNDLINNQ MKPGIFWKKD
610 620 630 640 650
FISAIKFDGF TSIISLESLH QLLSIHYRIT LQVLLTFVLF DLDTEIFGQH
660 670 680 690 700
ISTLLDLHYK QFLLLNLYRQ DKCLLAEVLL KDSSEFSFGV KFFNYGQLIA
710 720 730 740 750
YIDSLNSNVY NASITENSFF MTFFRSYIIE NTSHKNIRFF LENVECPFYL
760 770 780 790 800
RHNEVQEFMF AMTLFSCGNF DQSYEIFQLH DYPEAINDKL PTFLEDLKSE
810 820 830 840 850
NYHGDSIWKD LLCTFTVPYR HSAFYYQLSL LFDRNNSQEF ALKCISKSAE
860 870 880 890 900
YSLKEIQIEE LQDFKEKQHI HYLNLLIHFR MFEEVLDVLR LGHECLSDTV
910 920 930 940 950
RTNFLQLLLQ EDIYSRDFFS TLLRLCNAHS DNGELYLRTV DIKIVDSILS
960 970 980 990 1000
QNLRSGDWEC FKKLYCFRML NKSERAAAEV LYQYILMQAD LDVIRKRKCY
1010 1020 1030
LMVINVLSSF DSAYDQWILN GSKVVTLTDL RDELRGL
Length:1,037
Mass (Da):120,448
Last modified:June 1, 1994 - v1
Checksum:iD4655E6116C54503
GO

Sequence cautioni

The sequence CAA53415.1 differs from that shown. Reason: Frameshift at position 442.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75781 Genomic DNA. Translation: CAA53414.1. Frameshift.
X75781 Genomic DNA. Translation: CAA53415.1. Frameshift.
Z28057 Genomic DNA. Translation: CAA81894.1.
BK006944 Genomic DNA. Translation: DAA09100.1.
PIRiS37879.
RefSeqiNP_012866.1. NM_001179623.1.

Genome annotation databases

EnsemblFungiiYKL057C; YKL057C; YKL057C.
GeneIDi853808.
KEGGisce:YKL057C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75781 Genomic DNA. Translation: CAA53414.1 . Frameshift.
X75781 Genomic DNA. Translation: CAA53415.1 . Frameshift.
Z28057 Genomic DNA. Translation: CAA81894.1 .
BK006944 Genomic DNA. Translation: DAA09100.1 .
PIRi S37879.
RefSeqi NP_012866.1. NM_001179623.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3F7F X-ray 2.60 A/B/C/D 1-729 [» ]
3H7N X-ray 3.00 A/B/C/D 1-729 [» ]
3HXR X-ray 3.00 A 1-757 [» ]
ProteinModelPortali P35729.
SMRi P35729. Positions 1-729.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34076. 204 interactions.
DIPi DIP-2721N.
IntActi P35729. 17 interactions.
MINTi MINT-491139.
STRINGi 4932.YKL057C.

Protein family/group databases

TCDBi 1.I.1.1.1. the nuclear pore complex (npc) family.

Proteomic databases

MaxQBi P35729.
PaxDbi P35729.
PeptideAtlasi P35729.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL057C ; YKL057C ; YKL057C .
GeneIDi 853808.
KEGGi sce:YKL057C.

Organism-specific databases

CYGDi YKL057c.
SGDi S000001540. NUP120.

Phylogenomic databases

eggNOGi NOG19505.
HOGENOMi HOG000113870.
InParanoidi P35729.
OMAi CHLKIWD.
OrthoDBi EOG7MKWFP.

Enzyme and pathway databases

BioCyci YEAST:G3O-31856-MONOMER.

Miscellaneous databases

EvolutionaryTracei P35729.
NextBioi 974969.

Gene expression databases

Genevestigatori P35729.

Family and domain databases

InterProi IPR021717. Nucleoporin_Nup160.
[Graphical view ]
Pfami PF11715. Nup160. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins."
    Rasmussen S.W.
    Yeast 10:S63-S68(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport."
    Aitchison J.D., Blobel G., Rout M.P.
    J. Cell Biol. 131:1659-1676(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 189-206 AND 800-807, CHARACTERIZATION, NUCLEAR MRNA EXPORT.
  5. "Nuclear pore complex clustering and nuclear accumulation of poly(A)+ RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene."
    Heath C.V., Copeland C.S., Amberg D.C., Del Priore V., Snyder M., Cole C.N.
    J. Cell Biol. 131:1677-1697(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, NPC ASSEMBLY AND DISTRIBUTION.
  6. "A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores."
    Siniossoglou S., Wimmer C., Rieger M., Doye V., Tekotte H., Weise C., Emig S., Segref A., Hurt E.C.
    Cell 84:265-275(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, NUCLEAR ENVELOPE ORGANIZATION.
  7. "Factors affecting nuclear export of the 60S ribosomal subunit in vivo."
    Stage-Zimmermann T., Schmidt U., Silver P.A.
    Mol. Biol. Cell 11:3777-3789(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PRE-RIBOSOME EXPORT.
  8. "The yeast nuclear pore complex: composition, architecture, and transport mechanism."
    Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
    J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
  9. "Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins."
    Lutzmann M., Kunze R., Buerer A., Aebi U., Hurt E.C.
    EMBO J. 21:387-397(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, NUP84 NPC SUBCOMPLEX ASSEMBLY/STRUCTURE.
  10. "Nuclear accumulation of the small GTPase Gsp1p depends on nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the acetyl-CoA carboxylase Acc1p."
    Gao H., Sumanaweera N., Bailer S.M., Stochaj U.
    J. Biol. Chem. 278:25331-25340(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, NUCLEAR GSP1 IMPORT.
  11. "Peering through the pore: nuclear pore complex structure, assembly, and function."
    Suntharalingam M., Wente S.R.
    Dev. Cell 4:775-789(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-417, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNU120_YEAST
AccessioniPrimary (citable) accession number: P35729
Secondary accession number(s): D6VXN0, P35730
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3