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P35729 (NU120_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleoporin NUP120
Alternative name(s):
Nuclear pore protein NUP120
Gene names
Name:NUP120
Synonyms:RAT2
Ordered Locus Names:YKL057C
ORF Names:YKL313, YKL314
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1037 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP120 is involved in nuclear poly(A)+ RNA and pre-ribosome export, in GSP1 nuclear import, in NPC assembly and distribution, as well as in nuclear envelope organization. Ref.5 Ref.6 Ref.7 Ref.9 Ref.10

Subunit structure

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NUP120 is part of the heptameric 0.5 MDa autoassembling NUP84 NPC subcomplex (NUP84, NUP85, NUP120, NUP133, NUP145C, SEC13 and SEH1). Ref.8

Subcellular location

Nucleusnuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note: Symmetric distribution.

Sequence caution

The sequence CAA53415.1 differs from that shown. Reason: Frameshift at position 442.

Ontologies

Keywords
   Biological processmRNA transport
Protein transport
Translocation
Transport
   Cellular componentMembrane
Nuclear pore complex
Nucleus
   DomainCoiled coil
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processdouble-strand break repair

Inferred from mutant phenotype PubMed 16418532PubMed 17538013. Source: SGD

mRNA export from nucleus

Inferred from mutant phenotype Ref.4Ref.5Ref.6. Source: SGD

mRNA export from nucleus in response to heat stress

Inferred from mutant phenotype PubMed 18258809. Source: SGD

maintenance of chromatin silencing at telomere

Inferred from mutant phenotype PubMed 16418532. Source: SGD

negative regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 22110603. Source: SGD

nuclear pore distribution

Inferred from mutant phenotype PubMed 22331846Ref.4Ref.5Ref.6PubMed 9725905. Source: SGD

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 22110603. Source: SGD

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 15817685. Source: SGD

posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery

Inferred from mutant phenotype PubMed 20932479. Source: SGD

protein export from nucleus

Inferred from mutant phenotype Ref.4. Source: SGD

protein import into nucleus

Inferred from mutant phenotype Ref.10. Source: SGD

ribosomal large subunit export from nucleus

Inferred from mutant phenotype Ref.7. Source: SGD

telomere tethering at nuclear periphery

Inferred from mutant phenotype PubMed 16418532. Source: SGD

   Cellular_componentnuclear membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

nuclear pore

Inferred from direct assay Ref.8. Source: SGD

nuclear pore outer ring

Inferred from direct assay Ref.9. Source: SGD

   Molecular_functionstructural constituent of nuclear pore

Inferred from mutant phenotype PubMed 22331846. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NUP145P496873EBI-11713,EBI-11730
NUP85P466733EBI-11713,EBI-12345

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10371037Nucleoporin NUP120
PRO_0000204836

Regions

Region131 – 15222Leucine-zipper 1 Potential
Region290 – 31122Leucine-zipper 2 Potential

Amino acid modifications

Modified residue4171Phosphothreonine Ref.12

Secondary structure

............................................................................................................................. 1037
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P35729 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: D4655E6116C54503

FASTA1,037120,448
        10         20         30         40         50         60 
MACLSRIDAN LLQYYEKPEP NNTVDLYVSN NSNNNGLKEG DKSISTPVPQ PYGSEYSNCL 

        70         80         90        100        110        120 
LLSNSEYICY HFSSRSTLLT FYPLSDAYHG KTINIHLPNA SMNQRYTLTI QEVEQQLLVN 

       130        140        150        160        170        180 
VILKDGSFLT LQLPLSFLFS SANTLNGEWF HLQNPYDFTV RVPHFLFYVS PQFSVVFLED 

       190        200        210        220        230        240 
GGLLGLKKVD GVHYEPLLFN DNSYLKSLTR FFSRSSKSDY DSVISCKLFH ERYLIVLTQN 

       250        260        270        280        290        300 
CHLKIWDLTS FTLIQDYDMV SQSDSDPSHF RKVEAVGEYL SLYNNTLVTL LPLENGLFQM 

       310        320        330        340        350        360 
GTLLVDSSGI LTYTFQNNIP TNLSASAIWS IVDLVLTRPL ELNVEASYLN LIVLWKSGTA 

       370        380        390        400        410        420 
SKLQILNVND ESFKNYEWIE SVNKSLVDLQ SEHDLDIVTK TGDVERGFCN LKSRYGTQIF 

       430        440        450        460        470        480 
ERAQQILSEN KIIMAHNEDE EYLANLETIL RDVKTAFNEA SSITLYGDEI ILVNCFQPYN 

       490        500        510        520        530        540 
HSLYKLNTTV ENWFYNMHSE TDGSELFKYL RTLNGFASTL SNDVLRSISK KFLDIITGEL 

       550        560        570        580        590        600 
PDSMTTVEKF TDIFKNCLEN QFEITNLKIL FDELNSFDIP VVLNDLINNQ MKPGIFWKKD 

       610        620        630        640        650        660 
FISAIKFDGF TSIISLESLH QLLSIHYRIT LQVLLTFVLF DLDTEIFGQH ISTLLDLHYK 

       670        680        690        700        710        720 
QFLLLNLYRQ DKCLLAEVLL KDSSEFSFGV KFFNYGQLIA YIDSLNSNVY NASITENSFF 

       730        740        750        760        770        780 
MTFFRSYIIE NTSHKNIRFF LENVECPFYL RHNEVQEFMF AMTLFSCGNF DQSYEIFQLH 

       790        800        810        820        830        840 
DYPEAINDKL PTFLEDLKSE NYHGDSIWKD LLCTFTVPYR HSAFYYQLSL LFDRNNSQEF 

       850        860        870        880        890        900 
ALKCISKSAE YSLKEIQIEE LQDFKEKQHI HYLNLLIHFR MFEEVLDVLR LGHECLSDTV 

       910        920        930        940        950        960 
RTNFLQLLLQ EDIYSRDFFS TLLRLCNAHS DNGELYLRTV DIKIVDSILS QNLRSGDWEC 

       970        980        990       1000       1010       1020 
FKKLYCFRML NKSERAAAEV LYQYILMQAD LDVIRKRKCY LMVINVLSSF DSAYDQWILN 

      1030 
GSKVVTLTDL RDELRGL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins."
Rasmussen S.W.
Yeast 10:S63-S68(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport."
Aitchison J.D., Blobel G., Rout M.P.
J. Cell Biol. 131:1659-1676(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 189-206 AND 800-807, CHARACTERIZATION, NUCLEAR MRNA EXPORT.
[5]"Nuclear pore complex clustering and nuclear accumulation of poly(A)+ RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene."
Heath C.V., Copeland C.S., Amberg D.C., Del Priore V., Snyder M., Cole C.N.
J. Cell Biol. 131:1677-1697(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, NPC ASSEMBLY AND DISTRIBUTION.
[6]"A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores."
Siniossoglou S., Wimmer C., Rieger M., Doye V., Tekotte H., Weise C., Emig S., Segref A., Hurt E.C.
Cell 84:265-275(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, NUCLEAR ENVELOPE ORGANIZATION.
[7]"Factors affecting nuclear export of the 60S ribosomal subunit in vivo."
Stage-Zimmermann T., Schmidt U., Silver P.A.
Mol. Biol. Cell 11:3777-3789(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PRE-RIBOSOME EXPORT.
[8]"The yeast nuclear pore complex: composition, architecture, and transport mechanism."
Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
[9]"Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins."
Lutzmann M., Kunze R., Buerer A., Aebi U., Hurt E.C.
EMBO J. 21:387-397(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, NUP84 NPC SUBCOMPLEX ASSEMBLY/STRUCTURE.
[10]"Nuclear accumulation of the small GTPase Gsp1p depends on nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the acetyl-CoA carboxylase Acc1p."
Gao H., Sumanaweera N., Bailer S.M., Stochaj U.
J. Biol. Chem. 278:25331-25340(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, NUCLEAR GSP1 IMPORT.
[11]"Peering through the pore: nuclear pore complex structure, assembly, and function."
Suntharalingam M., Wente S.R.
Dev. Cell 4:775-789(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[12]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-417, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X75781 Genomic DNA. Translation: CAA53414.1. Frameshift.
X75781 Genomic DNA. Translation: CAA53415.1. Frameshift.
Z28057 Genomic DNA. Translation: CAA81894.1.
BK006944 Genomic DNA. Translation: DAA09100.1.
PIRS37879.
RefSeqNP_012866.1. NM_001179623.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3F7FX-ray2.60A/B/C/D1-729[»]
3H7NX-ray3.00A/B/C/D1-729[»]
3HXRX-ray3.00A1-757[»]
ProteinModelPortalP35729.
SMRP35729. Positions 1-729.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34076. 203 interactions.
DIPDIP-2721N.
IntActP35729. 17 interactions.
MINTMINT-491139.
STRING4932.YKL057C.

Protein family/group databases

TCDB1.I.1.1.1. the nuclear pore complex (npc) family.

Proteomic databases

PaxDbP35729.
PeptideAtlasP35729.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL057C; YKL057C; YKL057C.
GeneID853808.
KEGGsce:YKL057C.

Organism-specific databases

CYGDYKL057c.
SGDS000001540. NUP120.

Phylogenomic databases

eggNOGNOG19505.
HOGENOMHOG000113870.
OMAPYDFTVR.
OrthoDBEOG7MKWFP.

Enzyme and pathway databases

BioCycYEAST:G3O-31856-MONOMER.

Gene expression databases

GenevestigatorP35729.

Family and domain databases

InterProIPR021717. Nucleoporin_Nup160.
[Graphical view]
PfamPF11715. Nup160. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP35729.
NextBio974969.

Entry information

Entry nameNU120_YEAST
AccessionPrimary (citable) accession number: P35729
Secondary accession number(s): D6VXN0, P35730
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references