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Protein

Protein MPE1

Gene

MPE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri180 – 19718CCHC-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • pre-mRNA binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • mRNA cleavage Source: SGD
  • mRNA polyadenylation Source: SGD
  • protein ubiquitination Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31858-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MPE1
Gene namesi
Name:MPE1
Ordered Locus Names:YKL059C
ORF Names:YKL316
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XI

Organism-specific databases

CYGDiYKL059c.
EuPathDBiFungiDB:YKL059C.
SGDiS000001542. MPE1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • mRNA cleavage and polyadenylation specificity factor complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 441441Protein MPE1PRO_0000076265Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei221 – 2211Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35728.
PaxDbiP35728.
PeptideAtlasiP35728.

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1.1 Publication

Protein-protein interaction databases

BioGridi34074. 59 interactions.
DIPiDIP-6650N.
IntActiP35728. 61 interactions.
MINTiMINT-624427.

Structurei

3D structure databases

ProteinModelPortaliP35728.
SMRiP35728. Positions 3-78, 179-226, 281-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 7874DWNNPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CCHC-type zinc finger.PROSITE-ProRule annotation
Contains 1 DWNN domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri180 – 19718CCHC-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5222.
GeneTreeiENSGT00610000086096.
HOGENOMiHOG000162394.
InParanoidiP35728.
KOiK15541.
OMAiNRMLSES.
OrthoDBiEOG73JM66.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR014891. DWNN_domain.
IPR001878. Znf_CCHC.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF08783. DWNN. 1 hit.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF57756. SSF57756. 1 hit.
PROSITEiPS51282. DWNN. 1 hit.
PS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTIFYRFK SQRNTSRILF DGTGLTVFDL KREIIQENKL GDGTDFQLKI
60 70 80 90 100
YNPDTEEEYD DDAFVIPRST SVIVKRSPAI KSFSVHSRLK GNVGAAALGN
110 120 130 140 150
ATRYVTGRPR VLQKRQHTAT TTANVSGTTE EERIASMFAT QENQWEQTQE
160 170 180 190 200
EMSAATPVFF KSQTNKNSAQ ENEGPPPPGY MCYRCGGRDH WIKNCPTNSD
210 220 230 240 250
PNFEGKRIRR TTGIPKKFLK SIEIDPETMT PEEMAQRKIM ITDEGKFVVQ
260 270 280 290 300
VEDKQSWEDY QRKRENRQID GDETIWRKGH FKDLPDDLKC PLTGGLLRQP
310 320 330 340 350
VKTSKCCNID FSKEALENAL VESDFVCPNC ETRDILLDSL VPDQDKEKEV
360 370 380 390 400
ETFLKKQEEL HGSSKDGNQP ETKKMKLMDP TGTAGLNNNT SLPTSVNNGG
410 420 430 440
TPVPPVPLPF GIPPFPMFPM PFMPPTATIT NPHQADASPK K
Length:441
Mass (Da):49,647
Last modified:June 1, 1994 - v1
Checksum:i487567F6FD2C3F61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75781 Genomic DNA. Translation: CAA53413.1.
Z28059 Genomic DNA. Translation: CAA81896.1.
BK006944 Genomic DNA. Translation: DAA09098.1.
PIRiS37881.
RefSeqiNP_012864.1. NM_001179625.1.

Genome annotation databases

EnsemblFungiiYKL059C; YKL059C; YKL059C.
GeneIDi853806.
KEGGisce:YKL059C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75781 Genomic DNA. Translation: CAA53413.1.
Z28059 Genomic DNA. Translation: CAA81896.1.
BK006944 Genomic DNA. Translation: DAA09098.1.
PIRiS37881.
RefSeqiNP_012864.1. NM_001179625.1.

3D structure databases

ProteinModelPortaliP35728.
SMRiP35728. Positions 3-78, 179-226, 281-352.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34074. 59 interactions.
DIPiDIP-6650N.
IntActiP35728. 61 interactions.
MINTiMINT-624427.

Proteomic databases

MaxQBiP35728.
PaxDbiP35728.
PeptideAtlasiP35728.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL059C; YKL059C; YKL059C.
GeneIDi853806.
KEGGisce:YKL059C.

Organism-specific databases

CYGDiYKL059c.
EuPathDBiFungiDB:YKL059C.
SGDiS000001542. MPE1.

Phylogenomic databases

eggNOGiCOG5222.
GeneTreeiENSGT00610000086096.
HOGENOMiHOG000162394.
InParanoidiP35728.
KOiK15541.
OMAiNRMLSES.
OrthoDBiEOG73JM66.

Enzyme and pathway databases

BioCyciYEAST:G3O-31858-MONOMER.

Miscellaneous databases

NextBioi974963.
PROiP35728.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR014891. DWNN_domain.
IPR001878. Znf_CCHC.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF08783. DWNN. 1 hit.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF57756. SSF57756. 1 hit.
PROSITEiPS51282. DWNN. 1 hit.
PS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins."
    Rasmussen S.W.
    Yeast 10:S63-S68(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends."
    Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J.
    J. Biol. Chem. 278:33000-33010(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CPF COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-221, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMPE1_YEAST
AccessioniPrimary (citable) accession number: P35728
Secondary accession number(s): D6VXM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 22, 2015
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1130 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.