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P35728

- MPE1_YEAST

UniProt

P35728 - MPE1_YEAST

Protein

Protein MPE1

Gene

MPE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri180 – 19718CCHC-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. mRNA cleavage Source: SGD
    2. mRNA polyadenylation Source: SGD

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31858-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein MPE1
    Gene namesi
    Name:MPE1
    Ordered Locus Names:YKL059C
    ORF Names:YKL316
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL059c.
    SGDiS000001542. MPE1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. mRNA cleavage and polyadenylation specificity factor complex Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 441441Protein MPE1PRO_0000076265Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei221 – 2211Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP35728.
    PaxDbiP35728.
    PeptideAtlasiP35728.

    Expressioni

    Gene expression databases

    GenevestigatoriP35728.

    Interactioni

    Subunit structurei

    Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1.1 Publication

    Protein-protein interaction databases

    BioGridi34074. 55 interactions.
    DIPiDIP-6650N.
    IntActiP35728. 61 interactions.
    MINTiMINT-624427.
    STRINGi4932.YKL059C.

    Structurei

    3D structure databases

    ProteinModelPortaliP35728.
    SMRiP35728. Positions 3-78, 179-226, 284-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 7874DWNNPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CCHC-type zinc finger.PROSITE-ProRule annotation
    Contains 1 DWNN domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri180 – 19718CCHC-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5222.
    GeneTreeiENSGT00610000086096.
    HOGENOMiHOG000162394.
    KOiK15541.
    OMAiNCETRDI.
    OrthoDBiEOG73JM66.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR014891. DWNN_domain.
    IPR001878. Znf_CCHC.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF08783. DWNN. 1 hit.
    [Graphical view]
    SMARTiSM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    SUPFAMiSSF57756. SSF57756. 1 hit.
    PROSITEiPS51282. DWNN. 1 hit.
    PS50158. ZF_CCHC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35728-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSTIFYRFK SQRNTSRILF DGTGLTVFDL KREIIQENKL GDGTDFQLKI    50
    YNPDTEEEYD DDAFVIPRST SVIVKRSPAI KSFSVHSRLK GNVGAAALGN 100
    ATRYVTGRPR VLQKRQHTAT TTANVSGTTE EERIASMFAT QENQWEQTQE 150
    EMSAATPVFF KSQTNKNSAQ ENEGPPPPGY MCYRCGGRDH WIKNCPTNSD 200
    PNFEGKRIRR TTGIPKKFLK SIEIDPETMT PEEMAQRKIM ITDEGKFVVQ 250
    VEDKQSWEDY QRKRENRQID GDETIWRKGH FKDLPDDLKC PLTGGLLRQP 300
    VKTSKCCNID FSKEALENAL VESDFVCPNC ETRDILLDSL VPDQDKEKEV 350
    ETFLKKQEEL HGSSKDGNQP ETKKMKLMDP TGTAGLNNNT SLPTSVNNGG 400
    TPVPPVPLPF GIPPFPMFPM PFMPPTATIT NPHQADASPK K 441
    Length:441
    Mass (Da):49,647
    Last modified:June 1, 1994 - v1
    Checksum:i487567F6FD2C3F61
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75781 Genomic DNA. Translation: CAA53413.1.
    Z28059 Genomic DNA. Translation: CAA81896.1.
    BK006944 Genomic DNA. Translation: DAA09098.1.
    PIRiS37881.
    RefSeqiNP_012864.1. NM_001179625.1.

    Genome annotation databases

    EnsemblFungiiYKL059C; YKL059C; YKL059C.
    GeneIDi853806.
    KEGGisce:YKL059C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75781 Genomic DNA. Translation: CAA53413.1 .
    Z28059 Genomic DNA. Translation: CAA81896.1 .
    BK006944 Genomic DNA. Translation: DAA09098.1 .
    PIRi S37881.
    RefSeqi NP_012864.1. NM_001179625.1.

    3D structure databases

    ProteinModelPortali P35728.
    SMRi P35728. Positions 3-78, 179-226, 284-355.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34074. 55 interactions.
    DIPi DIP-6650N.
    IntActi P35728. 61 interactions.
    MINTi MINT-624427.
    STRINGi 4932.YKL059C.

    Proteomic databases

    MaxQBi P35728.
    PaxDbi P35728.
    PeptideAtlasi P35728.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL059C ; YKL059C ; YKL059C .
    GeneIDi 853806.
    KEGGi sce:YKL059C.

    Organism-specific databases

    CYGDi YKL059c.
    SGDi S000001542. MPE1.

    Phylogenomic databases

    eggNOGi COG5222.
    GeneTreei ENSGT00610000086096.
    HOGENOMi HOG000162394.
    KOi K15541.
    OMAi NCETRDI.
    OrthoDBi EOG73JM66.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31858-MONOMER.

    Miscellaneous databases

    NextBioi 974963.
    PROi P35728.

    Gene expression databases

    Genevestigatori P35728.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR014891. DWNN_domain.
    IPR001878. Znf_CCHC.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF08783. DWNN. 1 hit.
    [Graphical view ]
    SMARTi SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57756. SSF57756. 1 hit.
    PROSITEi PS51282. DWNN. 1 hit.
    PS50158. ZF_CCHC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins."
      Rasmussen S.W.
      Yeast 10:S63-S68(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends."
      Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J.
      J. Biol. Chem. 278:33000-33010(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CPF COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-221, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMPE1_YEAST
    AccessioniPrimary (citable) accession number: P35728
    Secondary accession number(s): D6VXM8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1130 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3