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P35728 (MPE1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein MPE1
Gene names
Name:MPE1
Ordered Locus Names:YKL059C
ORF Names:YKL316
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length441 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB.

Subunit structure

Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Ref.4

Subcellular location

Nucleus Ref.4.

Miscellaneous

Present with 1130 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 CCHC-type zinc finger.

Contains 1 DWNN domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 441441Protein MPE1
PRO_0000076265

Regions

Domain5 – 7874DWNN
Zinc finger180 – 19718CCHC-type

Amino acid modifications

Modified residue2211Phosphoserine Ref.6

Sequences

Sequence LengthMass (Da)Tools
P35728 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 487567F6FD2C3F61

FASTA44149,647
        10         20         30         40         50         60 
MSSTIFYRFK SQRNTSRILF DGTGLTVFDL KREIIQENKL GDGTDFQLKI YNPDTEEEYD 

        70         80         90        100        110        120 
DDAFVIPRST SVIVKRSPAI KSFSVHSRLK GNVGAAALGN ATRYVTGRPR VLQKRQHTAT 

       130        140        150        160        170        180 
TTANVSGTTE EERIASMFAT QENQWEQTQE EMSAATPVFF KSQTNKNSAQ ENEGPPPPGY 

       190        200        210        220        230        240 
MCYRCGGRDH WIKNCPTNSD PNFEGKRIRR TTGIPKKFLK SIEIDPETMT PEEMAQRKIM 

       250        260        270        280        290        300 
ITDEGKFVVQ VEDKQSWEDY QRKRENRQID GDETIWRKGH FKDLPDDLKC PLTGGLLRQP 

       310        320        330        340        350        360 
VKTSKCCNID FSKEALENAL VESDFVCPNC ETRDILLDSL VPDQDKEKEV ETFLKKQEEL 

       370        380        390        400        410        420 
HGSSKDGNQP ETKKMKLMDP TGTAGLNNNT SLPTSVNNGG TPVPPVPLPF GIPPFPMFPM 

       430        440 
PFMPPTATIT NPHQADASPK K 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins."
Rasmussen S.W.
Yeast 10:S63-S68(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends."
Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J.
J. Biol. Chem. 278:33000-33010(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CPF COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-221, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X75781 Genomic DNA. Translation: CAA53413.1.
Z28059 Genomic DNA. Translation: CAA81896.1.
BK006944 Genomic DNA. Translation: DAA09098.1.
PIRS37881.
RefSeqNP_012864.1. NM_001179625.1.

3D structure databases

ProteinModelPortalP35728.
SMRP35728. Positions 3-78, 179-226, 278-341.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34074. 55 interactions.
DIPDIP-6650N.
IntActP35728. 61 interactions.
MINTMINT-624427.
STRING4932.YKL059C.

Proteomic databases

PaxDbP35728.
PeptideAtlasP35728.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL059C; YKL059C; YKL059C.
GeneID853806.
KEGGsce:YKL059C.

Organism-specific databases

CYGDYKL059c.
SGDS000001542. MPE1.

Phylogenomic databases

eggNOGCOG5222.
GeneTreeENSGT00610000086096.
HOGENOMHOG000162394.
KOK15541.
OMAANSNEEY.
OrthoDBEOG73JM66.

Enzyme and pathway databases

BioCycYEAST:G3O-31858-MONOMER.

Gene expression databases

GenevestigatorP35728.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR014891. DWNN_domain.
IPR001878. Znf_CCHC.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF08783. DWNN. 1 hit.
[Graphical view]
SMARTSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMSSF57756. SSF57756. 1 hit.
PROSITEPS51282. DWNN. 1 hit.
PS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974963.
PROP35728.

Entry information

Entry nameMPE1_YEAST
AccessionPrimary (citable) accession number: P35728
Secondary accession number(s): D6VXM8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families