Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein MPE1

Gene

MPE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB.

Miscellaneous

Present with 1130 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri180 – 197CCHC-typePROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

  • pre-mRNA binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • mRNA polyadenylation Source: SGD
  • pre-mRNA cleavage required for polyadenylation Source: SGD
  • protein ubiquitination Source: SGD

Keywordsi

Biological processmRNA processing
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31858-MONOMER
ReactomeiR-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MPE1
Gene namesi
Name:MPE1
Ordered Locus Names:YKL059C
ORF Names:YKL316
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL059C
SGDiS000001542 MPE1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000762651 – 441Protein MPE1Add BLAST441

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei221PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35728
PaxDbiP35728
PRIDEiP35728

PTM databases

iPTMnetiP35728

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34074, 237 interactors
DIPiDIP-6650N
IntActiP35728, 62 interactors
MINTiP35728
STRINGi4932.YKL059C

Structurei

3D structure databases

ProteinModelPortaliP35728
SMRiP35728
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 78DWNNPROSITE-ProRule annotationAdd BLAST74

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri180 – 197CCHC-typePROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00610000086096
HOGENOMiHOG000162394
InParanoidiP35728
KOiK15541
OMAiIANMFAN
OrthoDBiEOG092C3VJP

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR014891 DWNN_domain
IPR025829 Zn_knuckle_CX2CX3GHX4C
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF08783 DWNN, 1 hit
PF13696 zf-CCHC_2, 1 hit
SMARTiView protein in SMART
SM01180 DWNN, 1 hit
SM00343 ZnF_C2HC, 1 hit
SUPFAMiSSF57756 SSF57756, 1 hit
PROSITEiView protein in PROSITE
PS51282 DWNN, 1 hit
PS50158 ZF_CCHC, 1 hit

Sequencei

Sequence statusi: Complete.

P35728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTIFYRFK SQRNTSRILF DGTGLTVFDL KREIIQENKL GDGTDFQLKI
60 70 80 90 100
YNPDTEEEYD DDAFVIPRST SVIVKRSPAI KSFSVHSRLK GNVGAAALGN
110 120 130 140 150
ATRYVTGRPR VLQKRQHTAT TTANVSGTTE EERIASMFAT QENQWEQTQE
160 170 180 190 200
EMSAATPVFF KSQTNKNSAQ ENEGPPPPGY MCYRCGGRDH WIKNCPTNSD
210 220 230 240 250
PNFEGKRIRR TTGIPKKFLK SIEIDPETMT PEEMAQRKIM ITDEGKFVVQ
260 270 280 290 300
VEDKQSWEDY QRKRENRQID GDETIWRKGH FKDLPDDLKC PLTGGLLRQP
310 320 330 340 350
VKTSKCCNID FSKEALENAL VESDFVCPNC ETRDILLDSL VPDQDKEKEV
360 370 380 390 400
ETFLKKQEEL HGSSKDGNQP ETKKMKLMDP TGTAGLNNNT SLPTSVNNGG
410 420 430 440
TPVPPVPLPF GIPPFPMFPM PFMPPTATIT NPHQADASPK K
Length:441
Mass (Da):49,647
Last modified:June 1, 1994 - v1
Checksum:i487567F6FD2C3F61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75781 Genomic DNA Translation: CAA53413.1
Z28059 Genomic DNA Translation: CAA81896.1
BK006944 Genomic DNA Translation: DAA09098.1
PIRiS37881
RefSeqiNP_012864.1, NM_001179625.1

Genome annotation databases

EnsemblFungiiYKL059C; YKL059C; YKL059C
GeneIDi853806
KEGGisce:YKL059C

Similar proteinsi

Entry informationi

Entry nameiMPE1_YEAST
AccessioniPrimary (citable) accession number: P35728
Secondary accession number(s): D6VXM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health