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Protein

Manganese resistance protein MNR2

Gene

MNR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • magnesium ion transmembrane transporter activity Source: SGD

GO - Biological processi

  • cellular magnesium ion homeostasis Source: SGD
  • magnesium ion transmembrane transport Source: GOC
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31862-MONOMER.

Protein family/group databases

TCDBi1.A.35.2.2. the cora metal ion transporter (mit) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Manganese resistance protein MNR2
Gene namesi
Name:MNR2
Ordered Locus Names:YKL064W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL064W.
SGDiS000001547. MNR2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 912912CytoplasmicSequence analysisAdd
BLAST
Transmembranei913 – 93321HelicalSequence analysisAdd
BLAST
Topological domaini934 – 9418ExtracellularSequence analysis
Transmembranei942 – 96221HelicalSequence analysisAdd
BLAST
Topological domaini963 – 9697CytoplasmicSequence analysis

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • vacuole-mitochondrion membrane contact site Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 969969Manganese resistance protein MNR2PRO_0000201537Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei114 – 1141PhosphoserineCombined sources
Modified residuei175 – 1751PhosphoserineCombined sources
Modified residuei177 – 1771PhosphothreonineCombined sources
Modified residuei182 – 1821PhosphoserineCombined sources
Modified residuei383 – 3831PhosphoserineCombined sources
Modified residuei571 – 5711PhosphothreonineCombined sources
Modified residuei576 – 5761PhosphoserineCombined sources
Modified residuei582 – 5821PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35724.

PTM databases

iPTMnetiP35724.

Interactioni

Protein-protein interaction databases

BioGridi34069. 31 interactions.
DIPiDIP-8080N.
IntActiP35724. 5 interactions.
MINTiMINT-4492655.

Structurei

3D structure databases

ProteinModelPortaliP35724.
SMRiP35724. Positions 854-966.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000009621.
HOGENOMiHOG000066095.
InParanoidiP35724.
KOiK16073.
OMAiTKVNNDM.
OrthoDBiEOG71ZP97.

Family and domain databases

InterProiIPR002523. MgTranspt_CorA/ZnTranspt_ZntB.
[Graphical view]
PfamiPF01544. CorA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDNSQKDE GVPLLSPYSS SPQLRKKKRN QKRRKDKFVG HLKSDSRRPT
60 70 80 90 100
QLLHDNLQHN HGQITDFDQI DSWGMLHESD STSNDIIKSE DPSLKGAFID
110 120 130 140 150
HRPSMSQPRE GPQSVSSTVQ PQPIMKFSTP SYKKPAGLRP SDQNRSLVSD
160 170 180 190 200
LSPSELESWL KRRKSVHKSF VDENSPTDRR QSNANNDVVI DVDALMNHVN
210 220 230 240 250
NNASTGVNDN SKRRKKKRGS DDSSNKNSKS TSSDSNDEED EYNSRPSSSL
260 270 280 290 300
SSNNSSLDDV CLVLDDEGSE VPKAWPDCTV LEEFSKEETE RLRSQAIQDA
310 320 330 340 350
EAFHFQYDED EEDGTSNEDG ILFSKPIVTN IDVPELGNRR VNETENLKNG
360 370 380 390 400
RLRPKRIAPW HLIQRPMVLG SNSTKDSKSR IQSGLQDNLL VGRNIQYPPH
410 420 430 440 450
IISNNPEHFR FTYFRVDLDS TVHSPTISGL LQPGQKFQDL FVASIYSQDN
460 470 480 490 500
SAGHIKTHPN SPTPGIKAET VSQLQGLTAK NPSTLSSMSV ANIEDVPPFW
510 520 530 540 550
LDVSNPTEEE MKILSKAFGI HPLTTEDIFL GEVREKVELF RDYYLICFRS
560 570 580 590 600
FDIVAEKHVR RRRKEKQESA TLDHESISRR KSQAYGATMS NESNANNNNS
610 620 630 640 650
TSNASRSKWL PSILRARRRS SANRTTNTSS SSYKRRVKSE KKKMEENEKF
660 670 680 690 700
KRKSGDRHKP REGELEPLNV YIIVFRTGVL TFHFAPTPHP INVRRRARLL
710 720 730 740 750
KDYLNVTSDW IAYALIDDIT DAFAPMIELI EDEVYEIEDA ILKMHQSDDS
760 770 780 790 800
SDSDSSDSDS DSGASDEDAF PFDVYSKKTS YSSAKSSVSS RSMSTSEASF
810 820 830 840 850
NANLIGWKRK GDMLRRIGEC RKRVMSILRL LGSKADVIKG FAKRYNEQWE
860 870 880 890 900
ASPQSEIAMY LGDIQDHIVT MVSSLNHYEK LLSRSHSNYL AQINIDMTKV
910 920 930 940 950
NNDMNDVLGK ITILGTIVLP MNVITGLWGM NVIVPGQYRD SLTWFIGIVL
960
FMCMLACSAY MYTKRRFGF
Length:969
Mass (Da):109,737
Last modified:June 1, 1994 - v1
Checksum:i48D2125A1211104E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75781 Genomic DNA. Translation: CAA53410.1.
Z28064 Genomic DNA. Translation: CAA81901.1.
BK006944 Genomic DNA. Translation: DAA09093.1.
PIRiS37886.
RefSeqiNP_012859.1. NM_001179630.1.

Genome annotation databases

EnsemblFungiiYKL064W; YKL064W; YKL064W.
GeneIDi853801.
KEGGisce:YKL064W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75781 Genomic DNA. Translation: CAA53410.1.
Z28064 Genomic DNA. Translation: CAA81901.1.
BK006944 Genomic DNA. Translation: DAA09093.1.
PIRiS37886.
RefSeqiNP_012859.1. NM_001179630.1.

3D structure databases

ProteinModelPortaliP35724.
SMRiP35724. Positions 854-966.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34069. 31 interactions.
DIPiDIP-8080N.
IntActiP35724. 5 interactions.
MINTiMINT-4492655.

Protein family/group databases

TCDBi1.A.35.2.2. the cora metal ion transporter (mit) family.

PTM databases

iPTMnetiP35724.

Proteomic databases

MaxQBiP35724.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL064W; YKL064W; YKL064W.
GeneIDi853801.
KEGGisce:YKL064W.

Organism-specific databases

EuPathDBiFungiDB:YKL064W.
SGDiS000001547. MNR2.

Phylogenomic databases

GeneTreeiENSGT00390000009621.
HOGENOMiHOG000066095.
InParanoidiP35724.
KOiK16073.
OMAiTKVNNDM.
OrthoDBiEOG71ZP97.

Enzyme and pathway databases

BioCyciYEAST:G3O-31862-MONOMER.

Miscellaneous databases

NextBioi974948.
PROiP35724.

Family and domain databases

InterProiIPR002523. MgTranspt_CorA/ZnTranspt_ZntB.
[Graphical view]
PfamiPF01544. CorA. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins."
    Rasmussen S.W.
    Yeast 10:S63-S68(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-571 AND SER-576, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114 AND SER-175, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-175; THR-177; SER-182; SER-383 AND SER-582, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMNR2_YEAST
AccessioniPrimary (citable) accession number: P35724
Secondary accession number(s): D6VXM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 11, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.