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Protein

Neurogranin

Gene

NRGN

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei38 – 381Crucial for interaction with calmodulinBy similarity

Keywords - Ligandi

Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neurogranin
Short name:
NG
Alternative name(s):
B-50 immunoreactive C-kinase substrate
Short name:
BICKS
p17
Cleaved into the following chain:
Gene namesi
Name:NRGN
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 29

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7878NeurograninPRO_0000159589Add
BLAST
Peptidei55 – 7824NEUG(55-78)By similarityPRO_0000377700Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei36 – 361Phosphoserine; by PHK and PKC2 Publications
Modified residuei68 – 681Citrulline; partial1 Publication

Post-translational modificationi

The N-terminus is blocked.
Phosphorylated at Ser-36 by PHK and PKC. Phosphorylation prevents interaction with Calmodulin and interrupts several learning- and memory-associated functions (By similarity).By similarity

Keywords - PTMi

Acetylation, Citrullination, Phosphoprotein

Proteomic databases

PaxDbiP35722.
PRIDEiP35722.

PTM databases

iPTMnetiP35722.

Expressioni

Tissue specificityi

Is highly enriched in brain. Accumulates postsynaptically in dendritic spines of neostriatal neurons.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000001930.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 4924IQPROSITE-ProRule annotationAdd
BLAST
Domaini50 – 7829Collagen-likeAdd
BLAST

Domaini

Neurogranin is intrinsically unstructured; however, upon binding with CaM, The IQ domain adopts a helical conformation.By similarity

Sequence similaritiesi

Belongs to the neurogranin family.Curated
Contains 1 collagen-like domain.Curated
Contains 1 IQ domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410JNUP. Eukaryota.
ENOG41118RJ. LUCA.
GeneTreeiENSGT00440000039324.
HOGENOMiHOG000113769.
HOVERGENiHBG000470.
InParanoidiP35722.
OMAiPQESACS.
OrthoDBiEOG7BCNFV.
TreeFamiTF342962.

Family and domain databases

InterProiIPR000048. IQ_motif_EF-hand-BS.
[Graphical view]
PfamiPF00612. IQ. 1 hit.
[Graphical view]
SMARTiSM00015. IQ. 1 hit.
[Graphical view]
PROSITEiPS50096. IQ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDCCTESACS KPDDDILDIP LDDPGANAAA AKIQASFRGH MARKKIKSGE
60 70
RGRKGPGPGG PGGAGGARGG AGGGPSGD
Length:78
Mass (Da):7,549
Last modified:July 11, 2006 - v3
Checksum:i8E47CDA39F085794
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111K → C AA sequence (PubMed:1824695).Curated
Sequence conflicti78 – 781D → G AA sequence (PubMed:1824695).Curated

Mass spectrometryi

Molecular mass is 7837.1±0.5 Da from positions 1 - 78. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112751 mRNA. Translation: AAI12752.1.
PIRiA39034.
RefSeqiNP_001106784.1. NM_001113313.2.
UniGeneiBt.12331.

Genome annotation databases

EnsembliENSBTAT00000001930; ENSBTAP00000001930; ENSBTAG00000001474.
GeneIDi616955.
KEGGibta:616955.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112751 mRNA. Translation: AAI12752.1.
PIRiA39034.
RefSeqiNP_001106784.1. NM_001113313.2.
UniGeneiBt.12331.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000001930.

PTM databases

iPTMnetiP35722.

Proteomic databases

PaxDbiP35722.
PRIDEiP35722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000001930; ENSBTAP00000001930; ENSBTAG00000001474.
GeneIDi616955.
KEGGibta:616955.

Organism-specific databases

CTDi4900.

Phylogenomic databases

eggNOGiENOG410JNUP. Eukaryota.
ENOG41118RJ. LUCA.
GeneTreeiENSGT00440000039324.
HOGENOMiHOG000113769.
HOVERGENiHBG000470.
InParanoidiP35722.
OMAiPQESACS.
OrthoDBiEOG7BCNFV.
TreeFamiTF342962.

Family and domain databases

InterProiIPR000048. IQ_motif_EF-hand-BS.
[Graphical view]
PfamiPF00612. IQ. 1 hit.
[Graphical view]
SMARTiSM00015. IQ. 1 hit.
[Graphical view]
PROSITEiPS50096. IQ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Purification and characterization of a brain-specific protein kinase C substrate, neurogranin (p17). Identification of a consensus amino acid sequence between neurogranin and neuromodulin (GAP43) that corresponds to the protein kinase C phosphorylation site and the calmodulin-binding domain."
    Baudier J., Deloulme J.C., van Dorsselaer A., Black D., Matthes H.W.D.
    J. Biol. Chem. 266:229-237(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, PHOSPHORYLATION AT SER-36, ACETYLATION AT MET-1, MASS SPECTROMETRY.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.
  3. "Phosphorylase kinase phosphorylates the calmodulin-binding regulatory regions of neuronal tissue-specific proteins B-50 (GAP-43) and neurogranin."
    Paudel H.K., Zwiers H., Wang J.H.
    J. Biol. Chem. 268:6207-6213(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-36 BY PHK.
  4. "Identification and Characterization of citrulline-modified brain proteins by combining HCD and CID fragmentation."
    Jin Z., Fu Z., Yang J., Troncosco J., Everett A.D., Van Eyk J.E.
    Proteomics 13:2682-2691(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: CITRULLINATION AT ARG-68.

Entry informationi

Entry nameiNEUG_BOVIN
AccessioniPrimary (citable) accession number: P35722
Secondary accession number(s): Q2KI68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 11, 2006
Last modified: July 6, 2016
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.