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Protein

DNA-directed RNA polymerase III subunit RPC8

Gene

RPC25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNA. The RPC25/RPC8-RPC17/RPC9 subcomplex may bind Pol III transcripts emerging from the adjacent exit pore during elongation.

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • RNA splicing Source: EnsemblPlants
  • termination of RNA polymerase III transcription Source: Reactome
  • transcription initiation from RNA polymerase III promoter Source: SGD
  • tRNA transcription by RNA polymerase III Source: SGD

Keywordsi

Biological processTranscription

Enzyme and pathway databases

BioCyciYEAST:G3O-31919-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC8
Short name:
RNA polymerase III subunit C8
Alternative name(s):
DNA-directed RNA polymerase III 25 kDa polypeptide
RNA polymerase III subunit C25
Gene namesi
Name:RPC25
Ordered Locus Names:YKL144C
ORF Names:UNF1, YKL1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL144C
SGDiS000001627 RPC25

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739961 – 212DNA-directed RNA polymerase III subunit RPC8Add BLAST212

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei162PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35718
PaxDbiP35718
PRIDEiP35718

PTM databases

iPTMnetiP35718

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. RPC25/RPC8 and RPC17/RPC9 form a Pol III subcomplex.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33992, 466 interactors
DIPiDIP-1414N
IntActiP35718, 17 interactors
MINTiP35718
STRINGi4932.YKL144C

Structurei

Secondary structure

1212
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 13Combined sources12
Helixi15 – 17Combined sources3
Helixi22 – 34Combined sources13
Beta strandi37 – 39Combined sources3
Turni40 – 42Combined sources3
Beta strandi43 – 54Combined sources12
Beta strandi61 – 64Combined sources4
Beta strandi66 – 77Combined sources12
Beta strandi84 – 93Combined sources10
Beta strandi96 – 100Combined sources5
Turni102 – 104Combined sources3
Beta strandi108 – 111Combined sources4
Turni112 – 114Combined sources3
Beta strandi120 – 122Combined sources3
Turni123 – 126Combined sources4
Beta strandi127 – 131Combined sources5
Beta strandi133 – 135Combined sources3
Beta strandi137 – 140Combined sources4
Beta strandi142 – 144Combined sources3
Beta strandi145 – 155Combined sources11
Turni163 – 170Combined sources8
Beta strandi176 – 178Combined sources3
Beta strandi193 – 199Combined sources7
Turni203 – 206Combined sources4
Helixi208 – 210Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CKZX-ray3.20B/D1-212[»]
5FJ8electron microscopy3.90G1-212[»]
5FJ9electron microscopy4.60G1-212[»]
5FJAelectron microscopy4.65G1-212[»]
6EU0electron microscopy4.00G1-212[»]
6EU1electron microscopy3.40G1-212[»]
6EU2electron microscopy3.40G1-212[»]
6EU3electron microscopy3.30G1-212[»]
6F40electron microscopy3.70G1-212[»]
6F41electron microscopy4.30G1-212[»]
6F42electron microscopy5.50G1-212[»]
6F44electron microscopy4.20G1-212[»]
ProteinModelPortaliP35718
SMRiP35718
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35718

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000004383
HOGENOMiHOG000157370
InParanoidiP35718
KOiK03022
OMAiERAHFWL
OrthoDBiEOG092C4KGR

Family and domain databases

Gene3Di3.30.1490.120, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR013238 RNA_pol_III_Rbc25
IPR036898 RNA_pol_Rpb7-like_N_sf
IPR005576 RNA_pol_Rpb7_N
IPR004519 RNAP_E/RPC8
PfamiView protein in Pfam
PF08292 RNA_pol_Rbc25, 1 hit
PF03876 SHS2_Rpb7-N, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF88798 SSF88798, 1 hit
TIGRFAMsiTIGR00448 rpoE, 1 hit

Sequencei

Sequence statusi: Complete.

P35718-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFILSKIADL VRIPPDQFHR DTISAITHQL NNKFANKIIP NVGLCITIYD
60 70 80 90 100
LLTVEEGQLK PGDGSSYINV TFRAVVFKPF LGEIVTGWIS KCTAEGIKVS
110 120 130 140 150
LLGIFDDIFI PQNMLFEGCY YTPEESAWIW PMDEETKLYF DVNEKIRFRI
160 170 180 190 200
EREVFVDVKP KSPKERELEE RAQLENEIEG KNEETPQNEK PPAYALLGSC
210
QTDGMGLVSW WE
Length:212
Mass (Da):24,326
Last modified:June 1, 1994 - v1
Checksum:i7A2422D5B0CEEEC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11048 Genomic DNA Translation: AAA20241.1
Z25464 Genomic DNA Translation: CAA80954.1
Z28144 Genomic DNA Translation: CAA81985.1
BK006944 Genomic DNA Translation: DAA09018.1
PIRiS37973
RefSeqiNP_012778.1, NM_001179710.1

Genome annotation databases

EnsemblFungiiYKL144C; YKL144C; YKL144C
GeneIDi853713
KEGGisce:YKL144C

Similar proteinsi

Entry informationi

Entry nameiRPC8_YEAST
AccessioniPrimary (citable) accession number: P35718
Secondary accession number(s): D6VX52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health