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Protein

DNA-directed RNA polymerase III subunit RPC8

Gene

RPC25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNA. The RPC25/RPC8-RPC17/RPC9 subcomplex may bind Pol III transcripts emerging from the adjacent exit pore during elongation.

GO - Molecular functioni

GO - Biological processi

  • transcription initiation from RNA polymerase III promoter Source: SGD
  • tRNA transcription from RNA polymerase III promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-31919-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC8
Short name:
RNA polymerase III subunit C8
Alternative name(s):
DNA-directed RNA polymerase III 25 kDa polypeptide
RNA polymerase III subunit C25
Gene namesi
Name:RPC25
Ordered Locus Names:YKL144C
ORF Names:UNF1, YKL1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL144C.
SGDiS000001627. RPC25.

Subcellular locationi

GO - Cellular componenti

  • DNA-directed RNA polymerase III complex Source: SGD
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 212212DNA-directed RNA polymerase III subunit RPC8PRO_0000073996Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei162 – 1621PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35718.

PTM databases

iPTMnetiP35718.

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. RPC25/RPC8 and RPC17/RPC9 form a Pol III subcomplex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RPO31P040515EBI-15854,EBI-15810

Protein-protein interaction databases

BioGridi33992. 67 interactions.
DIPiDIP-1414N.
IntActiP35718. 14 interactions.
MINTiMINT-399757.

Structurei

Secondary structure

1
212
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 1312Combined sources
Helixi15 – 173Combined sources
Helixi22 – 3413Combined sources
Beta strandi37 – 393Combined sources
Turni40 – 423Combined sources
Beta strandi43 – 5412Combined sources
Beta strandi61 – 644Combined sources
Beta strandi66 – 7712Combined sources
Beta strandi84 – 9310Combined sources
Beta strandi96 – 1005Combined sources
Beta strandi108 – 1114Combined sources
Turni112 – 1143Combined sources
Beta strandi120 – 1223Combined sources
Turni123 – 1264Combined sources
Beta strandi127 – 1315Combined sources
Beta strandi137 – 1404Combined sources
Beta strandi145 – 15511Combined sources
Beta strandi193 – 1997Combined sources
Helixi208 – 2103Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CKZX-ray3.20B/D1-212[»]
5FJ8electron microscopy3.90G1-212[»]
5FJ9electron microscopy4.60G1-212[»]
5FJAelectron microscopy4.65G1-212[»]
ProteinModelPortaliP35718.
SMRiP35718. Positions 1-212.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35718.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000004383.
HOGENOMiHOG000157370.
InParanoidiP35718.
KOiK03022.
OMAiERAHFWL.
OrthoDBiEOG7X0VTZ.

Family and domain databases

Gene3Di3.30.1490.120. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR013238. RNA_pol_III_Rbc25.
IPR005576. RNA_pol_Rpb7_N.
IPR004519. RNAP_E/RPC8.
[Graphical view]
PfamiPF08292. RNA_pol_Rbc25. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.
TIGRFAMsiTIGR00448. rpoE. 1 hit.

Sequencei

Sequence statusi: Complete.

P35718-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFILSKIADL VRIPPDQFHR DTISAITHQL NNKFANKIIP NVGLCITIYD
60 70 80 90 100
LLTVEEGQLK PGDGSSYINV TFRAVVFKPF LGEIVTGWIS KCTAEGIKVS
110 120 130 140 150
LLGIFDDIFI PQNMLFEGCY YTPEESAWIW PMDEETKLYF DVNEKIRFRI
160 170 180 190 200
EREVFVDVKP KSPKERELEE RAQLENEIEG KNEETPQNEK PPAYALLGSC
210
QTDGMGLVSW WE
Length:212
Mass (Da):24,326
Last modified:June 1, 1994 - v1
Checksum:i7A2422D5B0CEEEC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11048 Genomic DNA. Translation: AAA20241.1.
Z25464 Genomic DNA. Translation: CAA80954.1.
Z28144 Genomic DNA. Translation: CAA81985.1.
BK006944 Genomic DNA. Translation: DAA09018.1.
PIRiS37973.
RefSeqiNP_012778.1. NM_001179710.1.

Genome annotation databases

EnsemblFungiiYKL144C; YKL144C; YKL144C.
GeneIDi853713.
KEGGisce:YKL144C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11048 Genomic DNA. Translation: AAA20241.1.
Z25464 Genomic DNA. Translation: CAA80954.1.
Z28144 Genomic DNA. Translation: CAA81985.1.
BK006944 Genomic DNA. Translation: DAA09018.1.
PIRiS37973.
RefSeqiNP_012778.1. NM_001179710.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CKZX-ray3.20B/D1-212[»]
5FJ8electron microscopy3.90G1-212[»]
5FJ9electron microscopy4.60G1-212[»]
5FJAelectron microscopy4.65G1-212[»]
ProteinModelPortaliP35718.
SMRiP35718. Positions 1-212.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33992. 67 interactions.
DIPiDIP-1414N.
IntActiP35718. 14 interactions.
MINTiMINT-399757.

PTM databases

iPTMnetiP35718.

Proteomic databases

MaxQBiP35718.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL144C; YKL144C; YKL144C.
GeneIDi853713.
KEGGisce:YKL144C.

Organism-specific databases

EuPathDBiFungiDB:YKL144C.
SGDiS000001627. RPC25.

Phylogenomic databases

GeneTreeiENSGT00390000004383.
HOGENOMiHOG000157370.
InParanoidiP35718.
KOiK03022.
OMAiERAHFWL.
OrthoDBiEOG7X0VTZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-31919-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP35718.
NextBioi974722.
PROiP35718.

Family and domain databases

Gene3Di3.30.1490.120. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR013238. RNA_pol_III_Rbc25.
IPR005576. RNA_pol_Rpb7_N.
IPR004519. RNAP_E/RPC8.
[Graphical view]
PfamiPF08292. RNA_pol_Rbc25. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.
TIGRFAMsiTIGR00448. rpoE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "C25, an essential RNA polymerase III subunit related to the RNA polymerase II subunit RPB7."
    Sadhale P.P., Woychik N.A.
    Mol. Cell. Biol. 14:6164-6170(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular cloning and physical analysis of an 8.2 kb segment of chromosome XI of Saccharomyces cerevisiae reveals five tightly linked genes."
    Abraham P.R., Mulder A., Van'T Riet J., Planta R.J., Raue H.A.
    Yeast 8:227-238(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The yeast RNA polymerase III transcription machinery: a paradigm for eukaryotic gene activation."
    Chedin S., Ferri M.L., Peyroche G., Andrau J.-C., Jourdain S., Lefebvre O., Werner M., Carles C., Sentenac A.
    Cold Spring Harb. Symp. Quant. Biol. 63:381-389(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON THE RNA POL III COMPLEX.
  6. "An Rpb4/Rpb7-like complex in yeast RNA polymerase III contains the orthologue of mammalian CGRP-RCP."
    Siaut M., Zaros C., Levivier E., Ferri M.L., Court M., Werner M., Callebaut I., Thuriaux P., Sentenac A., Conesa C.
    Mol. Cell. Biol. 23:195-205(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RPC17.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model."
    Jasiak A.J., Armache K.J., Martens B., Jansen R.P., Cramer P.
    Mol. Cell 23:71-81(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) IN COMPLEX WITH RPC17, 3D-STRUCTURE MODELING OF THE POL III CORE COMPLEX.

Entry informationi

Entry nameiRPC8_YEAST
AccessioniPrimary (citable) accession number: P35718
Secondary accession number(s): D6VX52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 11, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.