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Protein

DNA-directed RNA polymerase III subunit RPC8

Gene

RPC25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNA. The RPC25/RPC8-RPC17/RPC9 subcomplex may bind Pol III transcripts emerging from the adjacent exit pore during elongation.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-31919-MONOMER.
ReactomeiR-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-73980. RNA Polymerase III Transcription Termination.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC8
Short name:
RNA polymerase III subunit C8
Alternative name(s):
DNA-directed RNA polymerase III 25 kDa polypeptide
RNA polymerase III subunit C25
Gene namesi
Name:RPC25
Ordered Locus Names:YKL144C
ORF Names:UNF1, YKL1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL144C.
SGDiS000001627. RPC25.

Subcellular locationi

GO - Cellular componenti

  • DNA-directed RNA polymerase III complex Source: SGD
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739961 – 212DNA-directed RNA polymerase III subunit RPC8Add BLAST212

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei162PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35718.
PRIDEiP35718.

PTM databases

iPTMnetiP35718.

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. RPC25/RPC8 and RPC17/RPC9 form a Pol III subcomplex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RPO31P040515EBI-15854,EBI-15810

Protein-protein interaction databases

BioGridi33992. 67 interactors.
DIPiDIP-1414N.
IntActiP35718. 14 interactors.
MINTiMINT-399757.

Structurei

Secondary structure

1212
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 13Combined sources12
Helixi15 – 17Combined sources3
Helixi22 – 34Combined sources13
Beta strandi37 – 39Combined sources3
Turni40 – 42Combined sources3
Beta strandi43 – 54Combined sources12
Beta strandi61 – 64Combined sources4
Beta strandi66 – 77Combined sources12
Beta strandi84 – 93Combined sources10
Beta strandi96 – 100Combined sources5
Beta strandi108 – 111Combined sources4
Turni112 – 114Combined sources3
Beta strandi120 – 122Combined sources3
Turni123 – 126Combined sources4
Beta strandi127 – 131Combined sources5
Beta strandi137 – 140Combined sources4
Beta strandi145 – 155Combined sources11
Beta strandi193 – 199Combined sources7
Helixi208 – 210Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CKZX-ray3.20B/D1-212[»]
5FJ8electron microscopy3.90G1-212[»]
5FJ9electron microscopy4.60G1-212[»]
5FJAelectron microscopy4.65G1-212[»]
ProteinModelPortaliP35718.
SMRiP35718.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35718.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000004383.
HOGENOMiHOG000157370.
InParanoidiP35718.
KOiK03022.
OMAiKIRFRVE.
OrthoDBiEOG092C4KGR.

Family and domain databases

Gene3Di3.30.1490.120. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR013238. RNA_pol_III_Rbc25.
IPR005576. RNA_pol_Rpb7_N.
IPR004519. RNAP_E/RPC8.
[Graphical view]
PfamiPF08292. RNA_pol_Rbc25. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.
TIGRFAMsiTIGR00448. rpoE. 1 hit.

Sequencei

Sequence statusi: Complete.

P35718-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFILSKIADL VRIPPDQFHR DTISAITHQL NNKFANKIIP NVGLCITIYD
60 70 80 90 100
LLTVEEGQLK PGDGSSYINV TFRAVVFKPF LGEIVTGWIS KCTAEGIKVS
110 120 130 140 150
LLGIFDDIFI PQNMLFEGCY YTPEESAWIW PMDEETKLYF DVNEKIRFRI
160 170 180 190 200
EREVFVDVKP KSPKERELEE RAQLENEIEG KNEETPQNEK PPAYALLGSC
210
QTDGMGLVSW WE
Length:212
Mass (Da):24,326
Last modified:June 1, 1994 - v1
Checksum:i7A2422D5B0CEEEC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11048 Genomic DNA. Translation: AAA20241.1.
Z25464 Genomic DNA. Translation: CAA80954.1.
Z28144 Genomic DNA. Translation: CAA81985.1.
BK006944 Genomic DNA. Translation: DAA09018.1.
PIRiS37973.
RefSeqiNP_012778.1. NM_001179710.1.

Genome annotation databases

EnsemblFungiiYKL144C; YKL144C; YKL144C.
GeneIDi853713.
KEGGisce:YKL144C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11048 Genomic DNA. Translation: AAA20241.1.
Z25464 Genomic DNA. Translation: CAA80954.1.
Z28144 Genomic DNA. Translation: CAA81985.1.
BK006944 Genomic DNA. Translation: DAA09018.1.
PIRiS37973.
RefSeqiNP_012778.1. NM_001179710.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CKZX-ray3.20B/D1-212[»]
5FJ8electron microscopy3.90G1-212[»]
5FJ9electron microscopy4.60G1-212[»]
5FJAelectron microscopy4.65G1-212[»]
ProteinModelPortaliP35718.
SMRiP35718.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33992. 67 interactors.
DIPiDIP-1414N.
IntActiP35718. 14 interactors.
MINTiMINT-399757.

PTM databases

iPTMnetiP35718.

Proteomic databases

MaxQBiP35718.
PRIDEiP35718.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL144C; YKL144C; YKL144C.
GeneIDi853713.
KEGGisce:YKL144C.

Organism-specific databases

EuPathDBiFungiDB:YKL144C.
SGDiS000001627. RPC25.

Phylogenomic databases

GeneTreeiENSGT00390000004383.
HOGENOMiHOG000157370.
InParanoidiP35718.
KOiK03022.
OMAiKIRFRVE.
OrthoDBiEOG092C4KGR.

Enzyme and pathway databases

BioCyciYEAST:G3O-31919-MONOMER.
ReactomeiR-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-73980. RNA Polymerase III Transcription Termination.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

EvolutionaryTraceiP35718.
PROiP35718.

Family and domain databases

Gene3Di3.30.1490.120. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR013238. RNA_pol_III_Rbc25.
IPR005576. RNA_pol_Rpb7_N.
IPR004519. RNAP_E/RPC8.
[Graphical view]
PfamiPF08292. RNA_pol_Rbc25. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.
TIGRFAMsiTIGR00448. rpoE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRPC8_YEAST
AccessioniPrimary (citable) accession number: P35718
Secondary accession number(s): D6VX52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.