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P35710

- SOX5_MOUSE

UniProt

P35710 - SOX5_MOUSE

Protein

Transcription factor SOX-5

Gene

Sox5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (23 Apr 2003)
      Previous versions | rss
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    Functioni

    Binds specifically to the DNA sequence 5'-AACAAT-3'. Activates transcription of COL2A1 and AGC1 in vitro.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi556 – 62469HMG boxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. protein binding Source: MGI
    3. protein heterodimerization activity Source: MGI
    4. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: MGI
    5. sequence-specific DNA binding transcription factor activity Source: MGI
    6. transcription regulatory region DNA binding Source: MGI

    GO - Biological processi

    1. cartilage development Source: MGI
    2. cell fate commitment Source: MGI
    3. central nervous system neuron differentiation Source: MGI
    4. in utero embryonic development Source: MGI
    5. negative regulation of transcription, DNA-templated Source: MGI
    6. oligodendrocyte differentiation Source: MGI
    7. positive regulation of chondrocyte differentiation Source: UniProtKB
    8. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    9. regulation of timing of neuron differentiation Source: MGI
    10. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor SOX-5
    Gene namesi
    Name:Sox5
    Synonyms:Sox-5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:98367. Sox5.

    Subcellular locationi

    GO - Cellular componenti

    1. nuclear transcription factor complex Source: MGI
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 763763Transcription factor SOX-5PRO_0000048727Add
    BLAST

    Proteomic databases

    PRIDEiP35710.

    PTM databases

    PhosphoSiteiP35710.

    Expressioni

    Tissue specificityi

    Isoform 1 is found in the embryo and in adult testis. Isoform 2 is expressed in chondrocytes and, to a lesser extent, in brain. Isoform 3 is testis-specific.3 Publications

    Developmental stagei

    Expressed during spermatogenesis.

    Gene expression databases

    ArrayExpressiP35710.
    BgeeiP35710.
    CleanExiMM_SOX5.
    GenevestigatoriP35710.

    Interactioni

    Subunit structurei

    Forms homodimers and heterodimers with SOX6.1 Publication

    Protein-protein interaction databases

    BioGridi203409. 1 interaction.
    IntActiP35710. 1 interaction.

    Structurei

    Secondary structure

    1
    763
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi562 – 57514
    Helixi583 – 59412
    Helixi600 – 6023
    Helixi603 – 61715

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1I11NMR-A554-632[»]
    ProteinModelPortaliP35710.
    SMRiP35710. Positions 554-623.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP35710.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili193 – 27482Sequence AnalysisAdd
    BLAST
    Coiled coili448 – 51568Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG253815.
    HOGENOMiHOG000056455.
    HOVERGENiHBG003915.
    InParanoidiP35710.
    KOiK09269.
    PhylomeDBiP35710.

    Family and domain databases

    Gene3Di1.10.30.10. 1 hit.
    InterProiIPR009071. HMG_box_dom.
    [Graphical view]
    PfamiPF00505. HMG_box. 1 hit.
    [Graphical view]
    SMARTiSM00398. HMG. 1 hit.
    [Graphical view]
    SUPFAMiSSF47095. SSF47095. 1 hit.
    PROSITEiPS50118. HMG_BOX_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P35710-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLTDPDLPQE FERMSSKRPA SPYGETDGEV AMVTSRQKVE EEESERLPAF    50
    HLPLHVSFPN KPHSEEFQPV SLLTQETCGP RTPTVQHNTM EVDGNKVMSS 100
    LSPYNSSTSP QKAEEGGRQS GESVSSAALG TPERRKGSLA DVVDTLKQRK 150
    MEELIKNEPE DTPSIEKLLS KDWKDKLLAM GSGNFGEIKG TPESLAEKER 200
    QLMGMINQLT SLREQLLAAH DEQKKLAASQ IEKQRQQMEL AKQQQEQIAR 250
    QQQQLLQQQH KINLLQQQIQ VQGQLPPLMI PVFPPDQRTL AAAAQQGFLL 300
    PPGFSYKAGC SDPYPVQLIP TTMAAAAAAT PGLGPLQLQQ FYAAQLAAMQ 350
    VSPGGKLLGL PQGNLGAAVS PTSIHTDKST NSPPPKSKDE VAQPLNLSAK 400
    PKTSDGKSPA SPTSPHMPAL RINSGAGPLK ASVPAALASP SARVSTIGYL 450
    NDHDAVTKAI QEARQMKEQL RREQQALDGK VAVVNSIGLS NCRTEKEKTT 500
    LESLTQQLAV KQNEEGKFSH GMMDFNMSGD SDGSAGVSES RIYRESRGRG 550
    SNEPHIKRPM NAFMVWAKDE RRKILQAFPD MHNSNISKIL GSRWKAMTNL 600
    EKQPYYEEQA RLSKQHLEKY PDYKYKPRPK RTCLVDGKKL RIGEYKAIMR 650
    NRRQEMRQYF NVGQQAQIPI ATAGVVYPSA IAMAGMPSPH LPSEHSSVSS 700
    SPEPGMPVIQ STYGAKGEEP HIKEEIQAED INGEIYEEYD EEEEDPDVDY 750
    GSDSENHIAG QAN 763
    Length:763
    Mass (Da):84,090
    Last modified:April 23, 2003 - v2
    Checksum:iE38B1470ECCB1DE7
    GO
    Isoform 2 (identifier: P35710-2) [UniParc]FASTAAdd to Basket

    Also known as: L-Sox5

    The sequence of this isoform differs from the canonical sequence as follows:
         56-90: Missing.
         340-388: Missing.

    Show »
    Length:679
    Mass (Da):75,225
    Checksum:iBD550F7328EE9A1B
    GO
    Isoform 3 (identifier: P35710-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-322: Missing.
         340-388: Missing.

    Show »
    Length:392
    Mass (Da):43,234
    Checksum:iBF6A679800F916C8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti102 – 1021S → A in CAA09269. (PubMed:9755172)Curated
    Sequence conflicti679 – 6791S → G in CAA46608. (PubMed:1396566)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 322322Missing in isoform 3. 1 PublicationVSP_007265Add
    BLAST
    Alternative sequencei56 – 9035Missing in isoform 2. 1 PublicationVSP_007266Add
    BLAST
    Alternative sequencei340 – 38849Missing in isoform 2 and isoform 3. 2 PublicationsVSP_007267Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65657 mRNA. Translation: CAA46608.1.
    X65658 mRNA. Translation: CAA46609.1.
    AB006330 mRNA. Translation: BAA32567.1.
    AJ010604 mRNA. Translation: CAA09269.1.
    CCDSiCCDS39702.1. [P35710-1]
    CCDS57469.1. [P35710-2]
    PIRiS25195.
    RefSeqiNP_001230092.1. NM_001243163.1.
    UniGeneiMm.1752.

    Genome annotation databases

    EnsembliENSMUST00000111748; ENSMUSP00000107377; ENSMUSG00000041540. [P35710-3]
    GeneIDi20678.
    KEGGimmu:20678.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65657 mRNA. Translation: CAA46608.1 .
    X65658 mRNA. Translation: CAA46609.1 .
    AB006330 mRNA. Translation: BAA32567.1 .
    AJ010604 mRNA. Translation: CAA09269.1 .
    CCDSi CCDS39702.1. [P35710-1 ]
    CCDS57469.1. [P35710-2 ]
    PIRi S25195.
    RefSeqi NP_001230092.1. NM_001243163.1.
    UniGenei Mm.1752.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1I11 NMR - A 554-632 [» ]
    ProteinModelPortali P35710.
    SMRi P35710. Positions 554-623.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203409. 1 interaction.
    IntActi P35710. 1 interaction.

    Chemistry

    ChEMBLi CHEMBL2176780.

    PTM databases

    PhosphoSitei P35710.

    Proteomic databases

    PRIDEi P35710.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000111748 ; ENSMUSP00000107377 ; ENSMUSG00000041540 . [P35710-3 ]
    GeneIDi 20678.
    KEGGi mmu:20678.

    Organism-specific databases

    CTDi 6660.
    MGIi MGI:98367. Sox5.

    Phylogenomic databases

    eggNOGi NOG253815.
    HOGENOMi HOG000056455.
    HOVERGENi HBG003915.
    InParanoidi P35710.
    KOi K09269.
    PhylomeDBi P35710.

    Miscellaneous databases

    EvolutionaryTracei P35710.
    NextBioi 299173.
    PROi P35710.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P35710.
    Bgeei P35710.
    CleanExi MM_SOX5.
    Genevestigatori P35710.

    Family and domain databases

    Gene3Di 1.10.30.10. 1 hit.
    InterProi IPR009071. HMG_box_dom.
    [Graphical view ]
    Pfami PF00505. HMG_box. 1 hit.
    [Graphical view ]
    SMARTi SM00398. HMG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47095. SSF47095. 1 hit.
    PROSITEi PS50118. HMG_BOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An SRY-related gene expressed during spermatogenesis in the mouse encodes a sequence-specific DNA-binding protein."
      Denny P., Swift S., Connor F., Ashworth A.
      EMBO J. 11:3705-3712(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY.
      Tissue: Brain and Testis.
    2. "The mouse Sox5 gene encodes a protein containing the leucine zipper and the Q box."
      Hiraoka Y., Ogawa M., Sakai Y., Kido S., Aiso S.
      Biochim. Biophys. Acta 1399:40-46(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Tissue: Embryo.
    3. "A new long form of Sox5 (L-Sox5), Sox6 and Sox9 are coexpressed in chondrogenesis and cooperatively activate the type II collagen gene."
      Lefebvre V., Li P., de Crombrugghe B.
      EMBO J. 17:5718-5733(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
    4. "DNA binding and bending properties of the post-meiotically expressed Sry-related protein Sox-5."
      Connor F., O'Cary P.D., Read C.M., Preston N.S., Driscoll P.C., Denny P., Crane-Robinson C., Ashworth A.
      Nucleic Acids Res. 22:3339-3346(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "Solution structure and backbone dynamics of the DNA-binding domain of mouse Sox-5."
      Cary P.D., Read C.M., Davis B., Driscoll P.C., Crane-Robinson C.
      Protein Sci. 10:83-98(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 554-632.

    Entry informationi

    Entry nameiSOX5_MOUSE
    AccessioniPrimary (citable) accession number: P35710
    Secondary accession number(s): O88184, O89018
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: April 23, 2003
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3