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Protein

Transcription factor SOX-5

Gene

Sox5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to the DNA sequence 5'-AACAAT-3'. Activates transcription of COL2A1 and AGC1 in vitro.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi556 – 62469HMG boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • sequence-specific DNA binding RNA polymerase II transcription factor activity Source: MGI
  • sequence-specific DNA binding transcription factor activity Source: MGI
  • transcription regulatory region DNA binding Source: MGI

GO - Biological processi

  • cartilage development Source: MGI
  • cell fate commitment Source: MGI
  • cellular response to transforming growth factor beta stimulus Source: MGI
  • central nervous system neuron differentiation Source: MGI
  • in utero embryonic development Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • oligodendrocyte differentiation Source: MGI
  • positive regulation of cartilage development Source: MGI
  • positive regulation of chondrocyte differentiation Source: UniProtKB
  • positive regulation of mesenchymal stem cell differentiation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of timing of neuron differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SOX-5
Gene namesi
Name:Sox5
Synonyms:Sox-5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:98367. Sox5.

Subcellular locationi

GO - Cellular componenti

  • nuclear transcription factor complex Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 763763Transcription factor SOX-5PRO_0000048727Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei21 – 211PhosphoserineBy similarity
Modified residuei131 – 1311PhosphothreonineBy similarity
Modified residuei370 – 3701PhosphoserineBy similarity
Modified residuei372 – 3721PhosphothreonineBy similarity
Modified residuei414 – 4141PhosphoserineBy similarity
Modified residuei439 – 4391PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35710.
PRIDEiP35710.

PTM databases

PhosphoSiteiP35710.

Expressioni

Tissue specificityi

Isoform 1 is found in the embryo and in adult testis. Isoform 2 is expressed in chondrocytes and, to a lesser extent, in brain. Isoform 3 is testis-specific.3 Publications

Developmental stagei

Expressed during spermatogenesis.

Gene expression databases

BgeeiP35710.
CleanExiMM_SOX5.
ExpressionAtlasiP35710. baseline and differential.
GenevisibleiP35710. MM.

Interactioni

Subunit structurei

Forms homodimers and heterodimers with SOX6.1 Publication

Protein-protein interaction databases

BioGridi203409. 2 interactions.
IntActiP35710. 1 interaction.
STRINGi10090.ENSMUSP00000047567.

Structurei

Secondary structure

1
763
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi562 – 57514Combined sources
Helixi583 – 59412Combined sources
Helixi600 – 6023Combined sources
Helixi603 – 61715Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I11NMR-A554-632[»]
ProteinModelPortaliP35710.
SMRiP35710. Positions 554-623.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35710.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili193 – 27482Sequence AnalysisAdd
BLAST
Coiled coili448 – 51568Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG253815.
HOGENOMiHOG000056455.
HOVERGENiHBG003915.
InParanoidiP35710.
PhylomeDBiP35710.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P35710-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLTDPDLPQE FERMSSKRPA SPYGETDGEV AMVTSRQKVE EEESERLPAF
60 70 80 90 100
HLPLHVSFPN KPHSEEFQPV SLLTQETCGP RTPTVQHNTM EVDGNKVMSS
110 120 130 140 150
LSPYNSSTSP QKAEEGGRQS GESVSSAALG TPERRKGSLA DVVDTLKQRK
160 170 180 190 200
MEELIKNEPE DTPSIEKLLS KDWKDKLLAM GSGNFGEIKG TPESLAEKER
210 220 230 240 250
QLMGMINQLT SLREQLLAAH DEQKKLAASQ IEKQRQQMEL AKQQQEQIAR
260 270 280 290 300
QQQQLLQQQH KINLLQQQIQ VQGQLPPLMI PVFPPDQRTL AAAAQQGFLL
310 320 330 340 350
PPGFSYKAGC SDPYPVQLIP TTMAAAAAAT PGLGPLQLQQ FYAAQLAAMQ
360 370 380 390 400
VSPGGKLLGL PQGNLGAAVS PTSIHTDKST NSPPPKSKDE VAQPLNLSAK
410 420 430 440 450
PKTSDGKSPA SPTSPHMPAL RINSGAGPLK ASVPAALASP SARVSTIGYL
460 470 480 490 500
NDHDAVTKAI QEARQMKEQL RREQQALDGK VAVVNSIGLS NCRTEKEKTT
510 520 530 540 550
LESLTQQLAV KQNEEGKFSH GMMDFNMSGD SDGSAGVSES RIYRESRGRG
560 570 580 590 600
SNEPHIKRPM NAFMVWAKDE RRKILQAFPD MHNSNISKIL GSRWKAMTNL
610 620 630 640 650
EKQPYYEEQA RLSKQHLEKY PDYKYKPRPK RTCLVDGKKL RIGEYKAIMR
660 670 680 690 700
NRRQEMRQYF NVGQQAQIPI ATAGVVYPSA IAMAGMPSPH LPSEHSSVSS
710 720 730 740 750
SPEPGMPVIQ STYGAKGEEP HIKEEIQAED INGEIYEEYD EEEEDPDVDY
760
GSDSENHIAG QAN
Length:763
Mass (Da):84,090
Last modified:April 23, 2003 - v2
Checksum:iE38B1470ECCB1DE7
GO
Isoform 2 (identifier: P35710-2) [UniParc]FASTAAdd to basket

Also known as: L-Sox5

The sequence of this isoform differs from the canonical sequence as follows:
     56-90: Missing.
     340-388: Missing.

Show »
Length:679
Mass (Da):75,225
Checksum:iBD550F7328EE9A1B
GO
Isoform 3 (identifier: P35710-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-322: Missing.
     340-388: Missing.

Show »
Length:392
Mass (Da):43,234
Checksum:iBF6A679800F916C8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti102 – 1021S → A in CAA09269 (PubMed:9755172).Curated
Sequence conflicti679 – 6791S → G in CAA46608 (PubMed:1396566).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 322322Missing in isoform 3. 1 PublicationVSP_007265Add
BLAST
Alternative sequencei56 – 9035Missing in isoform 2. 1 PublicationVSP_007266Add
BLAST
Alternative sequencei340 – 38849Missing in isoform 2 and isoform 3. 2 PublicationsVSP_007267Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65657 mRNA. Translation: CAA46608.1.
X65658 mRNA. Translation: CAA46609.1.
AB006330 mRNA. Translation: BAA32567.1.
AJ010604 mRNA. Translation: CAA09269.1.
CCDSiCCDS39702.1. [P35710-1]
CCDS57469.1. [P35710-2]
PIRiS25195.
RefSeqiNP_001230092.1. NM_001243163.1.
UniGeneiMm.1752.

Genome annotation databases

GeneIDi20678.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65657 mRNA. Translation: CAA46608.1.
X65658 mRNA. Translation: CAA46609.1.
AB006330 mRNA. Translation: BAA32567.1.
AJ010604 mRNA. Translation: CAA09269.1.
CCDSiCCDS39702.1. [P35710-1]
CCDS57469.1. [P35710-2]
PIRiS25195.
RefSeqiNP_001230092.1. NM_001243163.1.
UniGeneiMm.1752.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I11NMR-A554-632[»]
ProteinModelPortaliP35710.
SMRiP35710. Positions 554-623.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203409. 2 interactions.
IntActiP35710. 1 interaction.
STRINGi10090.ENSMUSP00000047567.

Chemistry

ChEMBLiCHEMBL2176780.

PTM databases

PhosphoSiteiP35710.

Proteomic databases

MaxQBiP35710.
PRIDEiP35710.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20678.

Organism-specific databases

CTDi6660.
MGIiMGI:98367. Sox5.

Phylogenomic databases

eggNOGiNOG253815.
HOGENOMiHOG000056455.
HOVERGENiHBG003915.
InParanoidiP35710.
PhylomeDBiP35710.

Miscellaneous databases

EvolutionaryTraceiP35710.
NextBioi299173.
PROiP35710.
SOURCEiSearch...

Gene expression databases

BgeeiP35710.
CleanExiMM_SOX5.
ExpressionAtlasiP35710. baseline and differential.
GenevisibleiP35710. MM.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "An SRY-related gene expressed during spermatogenesis in the mouse encodes a sequence-specific DNA-binding protein."
    Denny P., Swift S., Connor F., Ashworth A.
    EMBO J. 11:3705-3712(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY.
    Tissue: Brain and Testis.
  2. "The mouse Sox5 gene encodes a protein containing the leucine zipper and the Q box."
    Hiraoka Y., Ogawa M., Sakai Y., Kido S., Aiso S.
    Biochim. Biophys. Acta 1399:40-46(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Embryo.
  3. "A new long form of Sox5 (L-Sox5), Sox6 and Sox9 are coexpressed in chondrogenesis and cooperatively activate the type II collagen gene."
    Lefebvre V., Li P., de Crombrugghe B.
    EMBO J. 17:5718-5733(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
  4. "DNA binding and bending properties of the post-meiotically expressed Sry-related protein Sox-5."
    Connor F., O'Cary P.D., Read C.M., Preston N.S., Driscoll P.C., Denny P., Crane-Robinson C., Ashworth A.
    Nucleic Acids Res. 22:3339-3346(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Solution structure and backbone dynamics of the DNA-binding domain of mouse Sox-5."
    Cary P.D., Read C.M., Davis B., Driscoll P.C., Crane-Robinson C.
    Protein Sci. 10:83-98(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 554-632.

Entry informationi

Entry nameiSOX5_MOUSE
AccessioniPrimary (citable) accession number: P35710
Secondary accession number(s): O88184, O89018
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: April 23, 2003
Last modified: July 22, 2015
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.