Reviewed, UniProtKB/Swiss-Prot P35710 (SOX5_MOUSE)

Names and origin

Protein names

Recommended name:
    Transcription factor SOX-5

Gene names

Name:	Sox5
Synonyms:	Sox-5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	763 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds specifically to the DNA sequence 5'-AACAAT-3'. Activates transcription of COL2A1 and AGC1 in vitro. Ref.3
Subunit structure	Forms homodimers and heterodimers with SOX6. Ref.3
Subcellular location	Nucleus.
Tissue specificity	Isoform 1 is found in the embryo and in adult testis. Isoform 2 is expressed in chondrocytes and, to a lesser extent, in brain. Isoform 3 is testis-specific. Ref.3 Ref.1 Ref.2
Developmental stage	Expressed during spermatogenesis.
Sequence similarities	Contains 1 HMG box DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cartilage development Inferred from genetic interaction. Source: MGI cell fate commitment Inferred from genetic interaction. Source: MGI central nervous system neuron differentiation Inferred from genetic interaction. Source: MGI positive regulation of transcription from RNA polymerase II promoter Ref.3 Inferred from genetic interaction. Source: MGI regulation of timing of neuron differentiation Inferred from mutant phenotype. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.3 Inferred from direct assay. Source: MGI
Molecular function	protein heterodimerization activity Ref.3 Inferred from physical interaction. Source: MGI transcription factor activity Ref.3 Inferred from genetic interaction. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P35710-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P35710-2)

Also known as: L-Sox5;

The sequence of this isoform differs from the canonical sequence as follows:
     56-90: Missing.
     340-388: Missing.

Isoform 3 (identifier: P35710-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-322: Missing.
     340-388: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 763

763

Transcription factor SOX-5

PRO_0000048727

Regions

DNA binding

556 – 624

69

HMG box

Coiled coil

193 – 274

82

Potential

Coiled coil

448 – 515

68

Potential

Amino acid modifications

Modified residue

108

1

Phosphothreonine Ref.5

Modified residue

414

1

Phosphoserine Ref.5

Natural variations

Alternative sequence

1 – 322

322

Missing in isoform 3.

VSP_007265

Alternative sequence

56 – 90

35

Missing in isoform 2.

VSP_007266

Alternative sequence

340 – 388

49

Missing in isoform 2 and isoform 3.

VSP_007267

Experimental info

Sequence conflict

102

1

S → A in CAA09269. Ref.3

Sequence conflict

679

1

S → G in CAA46608. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

763

Helix Strand Turn

Details...

Helix

562 – 575

14

Helix

583 – 594

12

Helix

600 – 602

3

Helix

603 – 617

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 23, 2003. Version 2. Checksum: E38B1470ECCB1DE7	FASTA	763	84,090
	10 20 30 40 50 60 MLTDPDLPQE FERMSSKRPA SPYGETDGEV AMVTSRQKVE EEESERLPAF HLPLHVSFPN 70 80 90 100 110 120 KPHSEEFQPV SLLTQETCGP RTPTVQHNTM EVDGNKVMSS LSPYNSSTSP QKAEEGGRQS 130 140 150 160 170 180 GESVSSAALG TPERRKGSLA DVVDTLKQRK MEELIKNEPE DTPSIEKLLS KDWKDKLLAM 190 200 210 220 230 240 GSGNFGEIKG TPESLAEKER QLMGMINQLT SLREQLLAAH DEQKKLAASQ IEKQRQQMEL 250 260 270 280 290 300 AKQQQEQIAR QQQQLLQQQH KINLLQQQIQ VQGQLPPLMI PVFPPDQRTL AAAAQQGFLL 310 320 330 340 350 360 PPGFSYKAGC SDPYPVQLIP TTMAAAAAAT PGLGPLQLQQ FYAAQLAAMQ VSPGGKLLGL 370 380 390 400 410 420 PQGNLGAAVS PTSIHTDKST NSPPPKSKDE VAQPLNLSAK PKTSDGKSPA SPTSPHMPAL 430 440 450 460 470 480 RINSGAGPLK ASVPAALASP SARVSTIGYL NDHDAVTKAI QEARQMKEQL RREQQALDGK 490 500 510 520 530 540 VAVVNSIGLS NCRTEKEKTT LESLTQQLAV KQNEEGKFSH GMMDFNMSGD SDGSAGVSES 550 560 570 580 590 600 RIYRESRGRG SNEPHIKRPM NAFMVWAKDE RRKILQAFPD MHNSNISKIL GSRWKAMTNL 610 620 630 640 650 660 EKQPYYEEQA RLSKQHLEKY PDYKYKPRPK RTCLVDGKKL RIGEYKAIMR NRRQEMRQYF 670 680 690 700 710 720 NVGQQAQIPI ATAGVVYPSA IAMAGMPSPH LPSEHSSVSS SPEPGMPVIQ STYGAKGEEP 730 740 750 760 HIKEEIQAED INGEIYEEYD EEEEDPDVDY GSDSENHIAG QAN
	Isoform 2 (L-Sox5). Checksum: BD550F7328EE9A1B	FASTA	679	75,225
	10 20 30 40 50 60 MLTDPDLPQE FERMSSKRPA SPYGETDGEV AMVTSRQKVE EEESERLPAF HLPLHEVDGN 70 80 90 100 110 120 KVMSSLSPYN SSTSPQKAEE GGRQSGESVS SAALGTPERR KGSLADVVDT LKQRKMEELI 130 140 150 160 170 180 KNEPEDTPSI EKLLSKDWKD KLLAMGSGNF GEIKGTPESL AEKERQLMGM INQLTSLREQ 190 200 210 220 230 240 LLAAHDEQKK LAASQIEKQR QQMELAKQQQ EQIARQQQQL LQQQHKINLL QQQIQVQGQL 250 260 270 280 290 300 PPLMIPVFPP DQRTLAAAAQ QGFLLPPGFS YKAGCSDPYP VQLIPTTMAA AAAATPGLGP 310 320 330 340 350 360 LQLQDEVAQP LNLSAKPKTS DGKSPASPTS PHMPALRINS GAGPLKASVP AALASPSARV 370 380 390 400 410 420 STIGYLNDHD AVTKAIQEAR QMKEQLRREQ QALDGKVAVV NSIGLSNCRT EKEKTTLESL 430 440 450 460 470 480 TQQLAVKQNE EGKFSHGMMD FNMSGDSDGS AGVSESRIYR ESRGRGSNEP HIKRPMNAFM 490 500 510 520 530 540 VWAKDERRKI LQAFPDMHNS NISKILGSRW KAMTNLEKQP YYEEQARLSK QHLEKYPDYK 550 560 570 580 590 600 YKPRPKRTCL VDGKKLRIGE YKAIMRNRRQ EMRQYFNVGQ QAQIPIATAG VVYPSAIAMA 610 620 630 640 650 660 GMPSPHLPSE HSSVSSSPEP GMPVIQSTYG AKGEEPHIKE EIQAEDINGE IYEEYDEEEE 670 DPDVDYGSDS ENHIAGQAN
	Isoform 3. Checksum: BF6A679800F916C8	FASTA	392	43,234
	10 20 30 40 50 60 MAAAAAATPG LGPLQLQDEV AQPLNLSAKP KTSDGKSPAS PTSPHMPALR INSGAGPLKA 70 80 90 100 110 120 SVPAALASPS ARVSTIGYLN DHDAVTKAIQ EARQMKEQLR REQQALDGKV AVVNSIGLSN 130 140 150 160 170 180 CRTEKEKTTL ESLTQQLAVK QNEEGKFSHG MMDFNMSGDS DGSAGVSESR IYRESRGRGS 190 200 210 220 230 240 NEPHIKRPMN AFMVWAKDER RKILQAFPDM HNSNISKILG SRWKAMTNLE KQPYYEEQAR 250 260 270 280 290 300 LSKQHLEKYP DYKYKPRPKR TCLVDGKKLR IGEYKAIMRN RRQEMRQYFN VGQQAQIPIA 310 320 330 340 350 360 TAGVVYPSAI AMAGMPSPHL PSEHSSVSSS PEPGMPVIQS TYGAKGEEPH IKEEIQAEDI 370 380 390 NGEIYEEYDE EEEDPDVDYG SDSENHIAGQ AN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An SRY-related gene expressed during spermatogenesis in the mouse encodes a sequence-specific DNA-binding protein." Denny P., Swift S., Connor F., Ashworth A. EMBO J. 11:3705-3712(1992) [PubMed: 1396566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY. Tissue: Brain and Testis.
[2]	"The mouse Sox5 gene encodes a protein containing the leucine zipper and the Q box." Hiraoka Y., Ogawa M., Sakai Y., Kido S., Aiso S. Biochim. Biophys. Acta 1399:40-46(1998) [PubMed: 9714725] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Embryo.
[3]	"A new long form of Sox5 (L-Sox5), Sox6 and Sox9 are coexpressed in chondrogenesis and cooperatively activate the type II collagen gene." Lefebvre V., Li P., de Crombrugghe B. EMBO J. 17:5718-5733(1998) [PubMed: 9755172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
[4]	"DNA binding and bending properties of the post-meiotically expressed Sry-related protein Sox-5." Connor F., O'Cary P.D., Read C.M., Preston N.S., Driscoll P.C., Denny P., Crane-Robinson C., Ashworth A. Nucleic Acids Res. 22:3339-3346(1994) [PubMed: 8078769] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-108 AND SER-414, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X65657 mRNA. Translation: CAA46608.1.
X65658 mRNA. Translation: CAA46609.1.
AB006330 mRNA. Translation: BAA32567.1.
AJ010604 mRNA. Translation: CAA09269.1.

IPI

IPI00128018.
IPI00466410.
IPI00467353.

PIR

S25195.

RefSeq

NP_001107031.1.
NP_035574.2.

UniGene

Mm.1752

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1I11	NMR	-	A	554-632	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P35710.

PTM databases

PhosphoSite

P35710.

Proteomic databases

PRIDE

P35710.

Genome annotation databases

Ensembl

ENSMUST00000038815; ENSMUSP00000047567; ENSMUSG00000041540; Mus musculus. [Genome view]
ENSMUST00000077160; ENSMUSP00000076403; ENSMUSG00000041540; Mus musculus. [Genome view]

GeneID

20678.

KEGG

mmu:20678.

UCSC

uc009eqk.1. mouse.
uc009eqm.1. mouse.

Organism-specific databases

MGI

MGI:98367. Sox5.

Phylogenomic databases

HOGENOM

P35710.

HOVERGEN

P35710.

Gene expression databases

ArrayExpress

P35710.

Bgee

P35710.

CleanEx

MM_SOX5.

Genevestigator

P35710.

GermOnline

ENSMUSG00000041540. Mus musculus.

Family and domain databases

InterPro

IPR000910. HMG_HMG1/HMG2.
[Graphical view]

Gene3D

G3DSA:1.10.30.10. HMG-box. 1 hit.

Pfam

PF00505. HMG_box. 1 hit.
[Graphical view]

SMART

SM00398. HMG. 1 hit.
[Graphical view]

PROSITE

PS50118. HMG_BOX_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

Entry information

Entry name

SOX5_MOUSE

Accession

Primary (citable) accession number: P35710
Secondary accession number(s): O88184, O89018

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	April 23, 2003
Last modified:	October 13, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)