Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription factor SOX-5

Gene

Sox5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to the DNA sequence 5'-AACAAT-3'. Activates transcription of COL2A1 and AGC1 in vitro.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi556 – 624HMG boxPROSITE-ProRule annotationAdd BLAST69

GO - Molecular functioni

  • DNA binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: MGI
  • transcription factor activity, sequence-specific DNA binding Source: MGI
  • transcription regulatory region DNA binding Source: MGI

GO - Biological processi

  • asymmetric neuroblast division Source: MGI
  • cartilage development Source: MGI
  • cell fate commitment Source: MGI
  • cellular response to transforming growth factor beta stimulus Source: MGI
  • central nervous system neuron differentiation Source: MGI
  • in utero embryonic development Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • oligodendrocyte differentiation Source: MGI
  • positive regulation of cartilage development Source: MGI
  • positive regulation of chondrocyte differentiation Source: UniProtKB
  • positive regulation of mesenchymal stem cell differentiation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of timing of neuron differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SOX-5
Gene namesi
Name:Sox5
Synonyms:Sox-5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:98367. Sox5.

Subcellular locationi

GO - Cellular componenti

  • nuclear transcription factor complex Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176780.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000487271 – 763Transcription factor SOX-5Add BLAST763

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei21PhosphoserineBy similarity1
Modified residuei131PhosphothreonineBy similarity1
Modified residuei370PhosphoserineBy similarity1
Modified residuei372PhosphothreonineBy similarity1
Modified residuei411PhosphoserineBy similarity1
Modified residuei414PhosphoserineBy similarity1
Modified residuei439PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35710.
PaxDbiP35710.
PeptideAtlasiP35710.
PRIDEiP35710.

PTM databases

iPTMnetiP35710.
PhosphoSitePlusiP35710.

Expressioni

Tissue specificityi

Isoform 1 is found in the embryo and in adult testis. Isoform 2 is expressed in chondrocytes and, to a lesser extent, in brain. Isoform 3 is testis-specific.3 Publications

Developmental stagei

Expressed during spermatogenesis.

Gene expression databases

BgeeiENSMUSG00000041540.
CleanExiMM_SOX5.
ExpressionAtlasiP35710. baseline and differential.
GenevisibleiP35710. MM.

Interactioni

Subunit structurei

Forms homodimers and heterodimers with SOX6.1 Publication

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi203409. 2 interactors.
IntActiP35710. 1 interactor.
STRINGi10090.ENSMUSP00000047567.

Structurei

Secondary structure

1763
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi562 – 575Combined sources14
Helixi583 – 594Combined sources12
Helixi600 – 602Combined sources3
Helixi603 – 617Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I11NMR-A554-632[»]
ProteinModelPortaliP35710.
SMRiP35710.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35710.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili193 – 274Sequence analysisAdd BLAST82
Coiled coili448 – 515Sequence analysisAdd BLAST68

Sequence similaritiesi

Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0528. Eukaryota.
ENOG410YZNG. LUCA.
GeneTreeiENSGT00760000119274.
HOGENOMiHOG000056455.
HOVERGENiHBG003915.
InParanoidiP35710.
KOiK09269.
PhylomeDBiP35710.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P35710-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLTDPDLPQE FERMSSKRPA SPYGETDGEV AMVTSRQKVE EEESERLPAF
60 70 80 90 100
HLPLHVSFPN KPHSEEFQPV SLLTQETCGP RTPTVQHNTM EVDGNKVMSS
110 120 130 140 150
LSPYNSSTSP QKAEEGGRQS GESVSSAALG TPERRKGSLA DVVDTLKQRK
160 170 180 190 200
MEELIKNEPE DTPSIEKLLS KDWKDKLLAM GSGNFGEIKG TPESLAEKER
210 220 230 240 250
QLMGMINQLT SLREQLLAAH DEQKKLAASQ IEKQRQQMEL AKQQQEQIAR
260 270 280 290 300
QQQQLLQQQH KINLLQQQIQ VQGQLPPLMI PVFPPDQRTL AAAAQQGFLL
310 320 330 340 350
PPGFSYKAGC SDPYPVQLIP TTMAAAAAAT PGLGPLQLQQ FYAAQLAAMQ
360 370 380 390 400
VSPGGKLLGL PQGNLGAAVS PTSIHTDKST NSPPPKSKDE VAQPLNLSAK
410 420 430 440 450
PKTSDGKSPA SPTSPHMPAL RINSGAGPLK ASVPAALASP SARVSTIGYL
460 470 480 490 500
NDHDAVTKAI QEARQMKEQL RREQQALDGK VAVVNSIGLS NCRTEKEKTT
510 520 530 540 550
LESLTQQLAV KQNEEGKFSH GMMDFNMSGD SDGSAGVSES RIYRESRGRG
560 570 580 590 600
SNEPHIKRPM NAFMVWAKDE RRKILQAFPD MHNSNISKIL GSRWKAMTNL
610 620 630 640 650
EKQPYYEEQA RLSKQHLEKY PDYKYKPRPK RTCLVDGKKL RIGEYKAIMR
660 670 680 690 700
NRRQEMRQYF NVGQQAQIPI ATAGVVYPSA IAMAGMPSPH LPSEHSSVSS
710 720 730 740 750
SPEPGMPVIQ STYGAKGEEP HIKEEIQAED INGEIYEEYD EEEEDPDVDY
760
GSDSENHIAG QAN
Length:763
Mass (Da):84,090
Last modified:April 23, 2003 - v2
Checksum:iE38B1470ECCB1DE7
GO
Isoform 2 (identifier: P35710-2) [UniParc]FASTAAdd to basket
Also known as: L-Sox5

The sequence of this isoform differs from the canonical sequence as follows:
     56-90: Missing.
     340-388: Missing.

Show »
Length:679
Mass (Da):75,225
Checksum:iBD550F7328EE9A1B
GO
Isoform 3 (identifier: P35710-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-322: Missing.
     340-388: Missing.

Show »
Length:392
Mass (Da):43,234
Checksum:iBF6A679800F916C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102S → A in CAA09269 (PubMed:9755172).Curated1
Sequence conflicti679S → G in CAA46608 (PubMed:1396566).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0072651 – 322Missing in isoform 3. 1 PublicationAdd BLAST322
Alternative sequenceiVSP_00726656 – 90Missing in isoform 2. 1 PublicationAdd BLAST35
Alternative sequenceiVSP_007267340 – 388Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65657 mRNA. Translation: CAA46608.1.
X65658 mRNA. Translation: CAA46609.1.
AB006330 mRNA. Translation: BAA32567.1.
AJ010604 mRNA. Translation: CAA09269.1.
CCDSiCCDS39702.1. [P35710-1]
CCDS57469.1. [P35710-2]
PIRiS25195.
RefSeqiNP_001230092.1. NM_001243163.1.
UniGeneiMm.1752.

Genome annotation databases

EnsembliENSMUST00000111748; ENSMUSP00000107377; ENSMUSG00000041540. [P35710-3]
GeneIDi20678.
KEGGimmu:20678.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65657 mRNA. Translation: CAA46608.1.
X65658 mRNA. Translation: CAA46609.1.
AB006330 mRNA. Translation: BAA32567.1.
AJ010604 mRNA. Translation: CAA09269.1.
CCDSiCCDS39702.1. [P35710-1]
CCDS57469.1. [P35710-2]
PIRiS25195.
RefSeqiNP_001230092.1. NM_001243163.1.
UniGeneiMm.1752.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I11NMR-A554-632[»]
ProteinModelPortaliP35710.
SMRiP35710.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203409. 2 interactors.
IntActiP35710. 1 interactor.
STRINGi10090.ENSMUSP00000047567.

Chemistry databases

ChEMBLiCHEMBL2176780.

PTM databases

iPTMnetiP35710.
PhosphoSitePlusiP35710.

Proteomic databases

MaxQBiP35710.
PaxDbiP35710.
PeptideAtlasiP35710.
PRIDEiP35710.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000111748; ENSMUSP00000107377; ENSMUSG00000041540. [P35710-3]
GeneIDi20678.
KEGGimmu:20678.

Organism-specific databases

CTDi6660.
MGIiMGI:98367. Sox5.

Phylogenomic databases

eggNOGiKOG0528. Eukaryota.
ENOG410YZNG. LUCA.
GeneTreeiENSGT00760000119274.
HOGENOMiHOG000056455.
HOVERGENiHBG003915.
InParanoidiP35710.
KOiK09269.
PhylomeDBiP35710.

Miscellaneous databases

EvolutionaryTraceiP35710.
PROiP35710.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041540.
CleanExiMM_SOX5.
ExpressionAtlasiP35710. baseline and differential.
GenevisibleiP35710. MM.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSOX5_MOUSE
AccessioniPrimary (citable) accession number: P35710
Secondary accession number(s): O88184, O89018
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: April 23, 2003
Last modified: November 2, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.