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P35710

- SOX5_MOUSE

UniProt

P35710 - SOX5_MOUSE

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Protein
Transcription factor SOX-5
Gene
Sox5, Sox-5
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Binds specifically to the DNA sequence 5'-AACAAT-3'. Activates transcription of COL2A1 and AGC1 in vitro.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi556 – 62469HMG box
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. protein binding Source: MGI
  3. protein heterodimerization activity Source: MGI
  4. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: MGI
  5. sequence-specific DNA binding transcription factor activity Source: MGI
  6. transcription regulatory region DNA binding Source: MGI
Complete GO annotation...

GO - Biological processi

  1. cartilage development Source: MGI
  2. cell fate commitment Source: MGI
  3. central nervous system neuron differentiation Source: MGI
  4. in utero embryonic development Source: MGI
  5. negative regulation of transcription, DNA-templated Source: MGI
  6. oligodendrocyte differentiation Source: MGI
  7. positive regulation of chondrocyte differentiation Source: UniProtKB
  8. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  9. regulation of timing of neuron differentiation Source: MGI
  10. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SOX-5
Gene namesi
Name:Sox5
Synonyms:Sox-5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:98367. Sox5.

Subcellular locationi

GO - Cellular componenti

  1. nuclear transcription factor complex Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 763763Transcription factor SOX-5
PRO_0000048727Add
BLAST

Proteomic databases

PRIDEiP35710.

PTM databases

PhosphoSiteiP35710.

Expressioni

Tissue specificityi

Isoform 1 is found in the embryo and in adult testis. Isoform 2 is expressed in chondrocytes and, to a lesser extent, in brain. Isoform 3 is testis-specific.3 Publications

Developmental stagei

Expressed during spermatogenesis.

Gene expression databases

ArrayExpressiP35710.
BgeeiP35710.
CleanExiMM_SOX5.
GenevestigatoriP35710.

Interactioni

Subunit structurei

Forms homodimers and heterodimers with SOX6.1 Publication

Protein-protein interaction databases

BioGridi203409. 1 interaction.
IntActiP35710. 1 interaction.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi562 – 57514
Helixi583 – 59412
Helixi600 – 6023
Helixi603 – 61715

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I11NMR-A554-632[»]
ProteinModelPortaliP35710.
SMRiP35710. Positions 554-623.

Miscellaneous databases

EvolutionaryTraceiP35710.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili193 – 27482 Reviewed prediction
Add
BLAST
Coiled coili448 – 51568 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG253815.
HOGENOMiHOG000056455.
HOVERGENiHBG003915.
InParanoidiP35710.
KOiK09269.
PhylomeDBiP35710.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P35710-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MLTDPDLPQE FERMSSKRPA SPYGETDGEV AMVTSRQKVE EEESERLPAF    50
HLPLHVSFPN KPHSEEFQPV SLLTQETCGP RTPTVQHNTM EVDGNKVMSS 100
LSPYNSSTSP QKAEEGGRQS GESVSSAALG TPERRKGSLA DVVDTLKQRK 150
MEELIKNEPE DTPSIEKLLS KDWKDKLLAM GSGNFGEIKG TPESLAEKER 200
QLMGMINQLT SLREQLLAAH DEQKKLAASQ IEKQRQQMEL AKQQQEQIAR 250
QQQQLLQQQH KINLLQQQIQ VQGQLPPLMI PVFPPDQRTL AAAAQQGFLL 300
PPGFSYKAGC SDPYPVQLIP TTMAAAAAAT PGLGPLQLQQ FYAAQLAAMQ 350
VSPGGKLLGL PQGNLGAAVS PTSIHTDKST NSPPPKSKDE VAQPLNLSAK 400
PKTSDGKSPA SPTSPHMPAL RINSGAGPLK ASVPAALASP SARVSTIGYL 450
NDHDAVTKAI QEARQMKEQL RREQQALDGK VAVVNSIGLS NCRTEKEKTT 500
LESLTQQLAV KQNEEGKFSH GMMDFNMSGD SDGSAGVSES RIYRESRGRG 550
SNEPHIKRPM NAFMVWAKDE RRKILQAFPD MHNSNISKIL GSRWKAMTNL 600
EKQPYYEEQA RLSKQHLEKY PDYKYKPRPK RTCLVDGKKL RIGEYKAIMR 650
NRRQEMRQYF NVGQQAQIPI ATAGVVYPSA IAMAGMPSPH LPSEHSSVSS 700
SPEPGMPVIQ STYGAKGEEP HIKEEIQAED INGEIYEEYD EEEEDPDVDY 750
GSDSENHIAG QAN 763
Length:763
Mass (Da):84,090
Last modified:April 23, 2003 - v2
Checksum:iE38B1470ECCB1DE7
GO
Isoform 2 (identifier: P35710-2) [UniParc]FASTAAdd to Basket

Also known as: L-Sox5

The sequence of this isoform differs from the canonical sequence as follows:
     56-90: Missing.
     340-388: Missing.

Show »
Length:679
Mass (Da):75,225
Checksum:iBD550F7328EE9A1B
GO
Isoform 3 (identifier: P35710-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-322: Missing.
     340-388: Missing.

Show »
Length:392
Mass (Da):43,234
Checksum:iBF6A679800F916C8
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 322322Missing in isoform 3.
VSP_007265Add
BLAST
Alternative sequencei56 – 9035Missing in isoform 2.
VSP_007266Add
BLAST
Alternative sequencei340 – 38849Missing in isoform 2 and isoform 3.
VSP_007267Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti102 – 1021S → A in CAA09269. 1 Publication
Sequence conflicti679 – 6791S → G in CAA46608. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65657 mRNA. Translation: CAA46608.1.
X65658 mRNA. Translation: CAA46609.1.
AB006330 mRNA. Translation: BAA32567.1.
AJ010604 mRNA. Translation: CAA09269.1.
CCDSiCCDS39702.1. [P35710-1]
CCDS57469.1. [P35710-2]
PIRiS25195.
RefSeqiNP_001230092.1. NM_001243163.1.
UniGeneiMm.1752.

Genome annotation databases

EnsembliENSMUST00000111748; ENSMUSP00000107377; ENSMUSG00000041540. [P35710-3]
GeneIDi20678.
KEGGimmu:20678.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65657 mRNA. Translation: CAA46608.1 .
X65658 mRNA. Translation: CAA46609.1 .
AB006330 mRNA. Translation: BAA32567.1 .
AJ010604 mRNA. Translation: CAA09269.1 .
CCDSi CCDS39702.1. [P35710-1 ]
CCDS57469.1. [P35710-2 ]
PIRi S25195.
RefSeqi NP_001230092.1. NM_001243163.1.
UniGenei Mm.1752.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1I11 NMR - A 554-632 [» ]
ProteinModelPortali P35710.
SMRi P35710. Positions 554-623.
ModBasei Search...

Protein-protein interaction databases

BioGridi 203409. 1 interaction.
IntActi P35710. 1 interaction.

Chemistry

ChEMBLi CHEMBL2176780.

PTM databases

PhosphoSitei P35710.

Proteomic databases

PRIDEi P35710.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000111748 ; ENSMUSP00000107377 ; ENSMUSG00000041540 . [P35710-3 ]
GeneIDi 20678.
KEGGi mmu:20678.

Organism-specific databases

CTDi 6660.
MGIi MGI:98367. Sox5.

Phylogenomic databases

eggNOGi NOG253815.
HOGENOMi HOG000056455.
HOVERGENi HBG003915.
InParanoidi P35710.
KOi K09269.
PhylomeDBi P35710.

Miscellaneous databases

EvolutionaryTracei P35710.
NextBioi 299173.
PROi P35710.
SOURCEi Search...

Gene expression databases

ArrayExpressi P35710.
Bgeei P35710.
CleanExi MM_SOX5.
Genevestigatori P35710.

Family and domain databases

Gene3Di 1.10.30.10. 1 hit.
InterProi IPR009071. HMG_box_dom.
[Graphical view ]
Pfami PF00505. HMG_box. 1 hit.
[Graphical view ]
SMARTi SM00398. HMG. 1 hit.
[Graphical view ]
SUPFAMi SSF47095. SSF47095. 1 hit.
PROSITEi PS50118. HMG_BOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "An SRY-related gene expressed during spermatogenesis in the mouse encodes a sequence-specific DNA-binding protein."
    Denny P., Swift S., Connor F., Ashworth A.
    EMBO J. 11:3705-3712(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY.
    Tissue: Brain and Testis.
  2. "The mouse Sox5 gene encodes a protein containing the leucine zipper and the Q box."
    Hiraoka Y., Ogawa M., Sakai Y., Kido S., Aiso S.
    Biochim. Biophys. Acta 1399:40-46(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Embryo.
  3. "A new long form of Sox5 (L-Sox5), Sox6 and Sox9 are coexpressed in chondrogenesis and cooperatively activate the type II collagen gene."
    Lefebvre V., Li P., de Crombrugghe B.
    EMBO J. 17:5718-5733(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
  4. "DNA binding and bending properties of the post-meiotically expressed Sry-related protein Sox-5."
    Connor F., O'Cary P.D., Read C.M., Preston N.S., Driscoll P.C., Denny P., Crane-Robinson C., Ashworth A.
    Nucleic Acids Res. 22:3339-3346(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Solution structure and backbone dynamics of the DNA-binding domain of mouse Sox-5."
    Cary P.D., Read C.M., Davis B., Driscoll P.C., Crane-Robinson C.
    Protein Sci. 10:83-98(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 554-632.

Entry informationi

Entry nameiSOX5_MOUSE
AccessioniPrimary (citable) accession number: P35710
Secondary accession number(s): O88184, O89018
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: April 23, 2003
Last modified: July 9, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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