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P35689

- ERCC5_MOUSE

UniProt

P35689 - ERCC5_MOUSE

Protein

DNA repair protein complementing XP-G cells homolog

Gene

Ercc5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 4 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too By similarity.By similarity

    Cofactori

    Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi30 – 301Magnesium 1By similarity
    Metal bindingi77 – 771Magnesium 1By similarity
    Metal bindingi788 – 7881Magnesium 1By similarity
    Metal bindingi790 – 7901Magnesium 1By similarity
    Metal bindingi809 – 8091Magnesium 2By similarity
    Metal bindingi811 – 8111Magnesium 2By similarity
    Metal bindingi860 – 8601Magnesium 2By similarity

    GO - Molecular functioni

    1. endonuclease activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. single-stranded DNA binding Source: InterPro

    GO - Biological processi

    1. determination of adult lifespan Source: MGI
    2. DNA repair Source: MGI
    3. multicellular organism growth Source: MGI
    4. nucleotide-excision repair Source: MGI
    5. response to UV Source: MGI
    6. UV protection Source: MGI

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA repair protein complementing XP-G cells homolog (EC:3.1.-.-)
    Alternative name(s):
    DNA excision repair protein ERCC-5
    Xeroderma pigmentosum group G-complementing protein homolog
    Gene namesi
    Name:Ercc5
    Synonyms:Ercc-5, Xpg
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:103582. Ercc5.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11701170DNA repair protein complementing XP-G cells homologPRO_0000154032Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei8 – 81N6-acetyllysineBy similarity
    Modified residuei384 – 3841Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiP35689.

    PTM databases

    PhosphoSiteiP35689.

    Expressioni

    Gene expression databases

    GenevestigatoriP35689.

    Interactioni

    Subunit structurei

    Interacts with PCNA.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP35689.
    SMRiP35689. Positions 2-127, 733-998.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 9595N-domainAdd
    BLAST
    Regioni752 – 882131I-domainAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1049 – 106517Nuclear localization signalSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0258.
    HOGENOMiHOG000131367.
    HOVERGENiHBG051501.
    InParanoidiP35689.

    Family and domain databases

    Gene3Di3.40.50.1010. 2 hits.
    InterProiIPR020045. 5-3_exonuclease_C.
    IPR008918. HhH2.
    IPR029060. PIN_domain-like.
    IPR006086. XPG-I_dom.
    IPR001044. XPG/Rad2_eukaryotes.
    IPR019974. XPG_CS.
    IPR006085. XPG_DNA_repair_N.
    [Graphical view]
    PfamiPF00867. XPG_I. 1 hit.
    PF00752. XPG_N. 1 hit.
    [Graphical view]
    PRINTSiPR00066. XRODRMPGMNTG.
    SMARTiSM00279. HhH2. 1 hit.
    SM00484. XPGI. 1 hit.
    SM00485. XPGN. 1 hit.
    [Graphical view]
    SUPFAMiSSF47807. SSF47807. 1 hit.
    SSF88723. SSF88723. 2 hits.
    TIGRFAMsiTIGR00600. rad2. 1 hit.
    PROSITEiPS00841. XPG_1. 1 hit.
    PS00842. XPG_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35689-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGVQGLWKLL ECSGHRVSPE ALEGKVLAVD ISIWLNQALK GVRDSHGNVI     50
    ENAHLLTLFH RLCKLLFFRI RPIFVFDGDA PLLKKQTLAK RRQRKDSASI 100
    DSRKTTEKLL KTFLKRQALK TAFRSSRHEA PPSLTQVQRQ DDIYVLPPLP 150
    EEEKHSSEEE DEKQWQARMD QKQALQEEFF HNPQAIDIES EDFSSLPPEV 200
    KHEILTDMKE FTKRRRTLFE AMPEESNDFS QYQLKGLLKK NYLNQHIENV 250
    QKEMNQQHSG QIQRQYQDEG GFLKEVESRR VVSEDTSHYI LIKGIQGKKV 300
    MDVDSESLPS SSNVHSVSSN LKSSPHEKVK PEREPEAAPP SPRTLLAIQA 350
    AMLGSSSEDE PESREGRQSK ERNSGATADA GSISPRTCAA IQKALDDDND 400
    EKVSGSSDDL AEKMLLGSGL EQEEHADETA ERGGGVPFDT APLTPSVTEV 450
    KECVTSGSSA NGQTDSAHSF TTASHRCDTP KETVSLARAV KEASQISSEC 500
    EVEGRPAALS PAFIGTPSSH VSGVLSEREP TLAPPTTRTH SDQGIDIHPE 550
    DPELQNGLYP LETKCNSSRL SSDDETEGGQ NPAPKACSTV HVPAEAMSNL 600
    ENALPSNAEE RGDFQETIQL REVPEAAARE LISAPKPMGP MEMESEESES 650
    DGSFIEVQSV VSNSELQTES SEASTHLSEK DAEEPRETLE EGTSRDTECL 700
    LQDSSDIEAM EGHREADIDA EDMPNEWQDI NLEELDALES NLLAEQNSLK 750
    AQKQQQDRIA ASVTGQMFLE SQELLRLFGV PYIQAPMEAE AQCAMLDLTD 800
    QTSGTITDDS DIWLFGARHV YKNFFNKNKF VEYYQYVDFY SQLGLDRNKL 850
    INLAYLLGSD YTEGIPTVGC VTAMEILNEF PGRGLDPLLK FSEWWHEAQN 900
    NKKVAENPYD TKVKKKLRKL QLTPGFPNPA VADAYLRPVV DDSRGSFLWG 950
    KPDVDKISTF CQRYFGWNRM KTDESLYPVL KHLNAHQTQL RIDSFFRLAQ 1000
    QEKQDAKLIK SHRLNRAVTC ILRKEREEKA PELTKVTEAL DDAKGKTQKR 1050
    ELPYKKETSV PKRRRPSGNG GFLGDPYCSE SPQESSCEDG EGSSVMSARQ 1100
    RSAAESSKIS CSDVPDLVRD PPHGRQGCVS TSSSSEDDED KAKTVLVTAR 1150
    PVFGKKKLKL KSMKRRKKKT 1170
    Length:1,170
    Mass (Da):130,715
    Last modified:October 3, 2012 - v4
    Checksum:iB4B9F93ACEBC3729
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti388 – 3881C → R in BAA03813. (PubMed:7590748)Curated
    Sequence conflicti419 – 4191G → R in BAA03813. (PubMed:7590748)Curated
    Sequence conflicti419 – 4191G → R in AAA91039. (PubMed:8703115)Curated
    Sequence conflicti419 – 4191G → R in AAB17885. (PubMed:8703115)Curated
    Sequence conflicti462 – 4621G → E in BAA03813. (PubMed:7590748)Curated
    Sequence conflicti462 – 4621G → E in AAA91039. (PubMed:8703115)Curated
    Sequence conflicti462 – 4621G → E in AAB17885. (PubMed:8703115)Curated
    Sequence conflicti488 – 4881R → S in BAA03813. (PubMed:7590748)Curated
    Sequence conflicti688 – 6881T → I in BAA03813. (PubMed:7590748)Curated
    Sequence conflicti799 – 7991T → S in BAA03813. (PubMed:7590748)Curated
    Sequence conflicti799 – 7991T → S in AAA91039. (PubMed:8703115)Curated
    Sequence conflicti799 – 7991T → S in AAB17885. (PubMed:8703115)Curated
    Sequence conflicti958 – 9592ST → RE in BAA03813. (PubMed:7590748)Curated
    Sequence conflicti958 – 9592ST → RE in AAA91039. (PubMed:8703115)Curated
    Sequence conflicti958 – 9592ST → RE in AAB17885. (PubMed:8703115)Curated
    Sequence conflicti1015 – 10151N → S in BAA03813. (PubMed:7590748)Curated
    Sequence conflicti1021 – 10211I → M in BAA03813. (PubMed:7590748)Curated
    Sequence conflicti1110 – 11101S → G in BAA03813. (PubMed:7590748)Curated
    Sequence conflicti1110 – 11101S → G in AAA91039. (PubMed:8703115)Curated
    Sequence conflicti1110 – 11101S → G in AAB17885. (PubMed:8703115)Curated
    Sequence conflicti1121 – 11211P → S in BAA03813. (PubMed:7590748)Curated
    Sequence conflicti1138 – 11381D → G in BAA03813. (PubMed:7590748)Curated
    Sequence conflicti1138 – 11381D → G in AAA91039. (PubMed:8703115)Curated
    Sequence conflicti1138 – 11381D → G in AAB17885. (PubMed:8703115)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16306 mRNA. Translation: BAA03813.1.
    U40796 mRNA. Translation: AAA91039.1.
    U40795
    , U39892, U39893, U39894, U39896, U40073, U40431, U40432, U40668, U40669, U40670, U40792, U40793, U40794 Genomic DNA. Translation: AAB17885.1.
    AC123800 Genomic DNA. No translation available.
    CCDSiCCDS35553.1.
    PIRiA57650.
    UniGeneiMm.2213.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16306 mRNA. Translation: BAA03813.1 .
    U40796 mRNA. Translation: AAA91039.1 .
    U40795
    , U39892 , U39893 , U39894 , U39896 , U40073 , U40431 , U40432 , U40668 , U40669 , U40670 , U40792 , U40793 , U40794 Genomic DNA. Translation: AAB17885.1 .
    AC123800 Genomic DNA. No translation available.
    CCDSi CCDS35553.1.
    PIRi A57650.
    UniGenei Mm.2213.

    3D structure databases

    ProteinModelPortali P35689.
    SMRi P35689. Positions 2-127, 733-998.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P35689.

    Proteomic databases

    PRIDEi P35689.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:103582. Ercc5.

    Phylogenomic databases

    eggNOGi COG0258.
    HOGENOMi HOG000131367.
    HOVERGENi HBG051501.
    InParanoidi P35689.

    Miscellaneous databases

    PROi P35689.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori P35689.

    Family and domain databases

    Gene3Di 3.40.50.1010. 2 hits.
    InterProi IPR020045. 5-3_exonuclease_C.
    IPR008918. HhH2.
    IPR029060. PIN_domain-like.
    IPR006086. XPG-I_dom.
    IPR001044. XPG/Rad2_eukaryotes.
    IPR019974. XPG_CS.
    IPR006085. XPG_DNA_repair_N.
    [Graphical view ]
    Pfami PF00867. XPG_I. 1 hit.
    PF00752. XPG_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00066. XRODRMPGMNTG.
    SMARTi SM00279. HhH2. 1 hit.
    SM00484. XPGI. 1 hit.
    SM00485. XPGN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47807. SSF47807. 1 hit.
    SSF88723. SSF88723. 2 hits.
    TIGRFAMsi TIGR00600. rad2. 1 hit.
    PROSITEi PS00841. XPG_1. 1 hit.
    PS00842. XPG_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An ERCC5 gene with homology to yeast RAD2 is involved in group G Xeroderma pigmentosum."
      Shiomi T., Harada Y.-N., Saito T., Shiomi N., Okuno Y., Yamaizumi M.
      Mutat. Res. 314:167-175(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Complementary DNA sequence and chromosomal localization of xpg, the mouse counterpart of human repair gene XPG/ERCC5."
      Harada Y.N., Matsuda Y., Shiomi N., Shiomi T.
      Genomics 28:59-65(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/10.
      Tissue: Liver.
    3. "Molecular cloning and structural analysis of the functional mouse genomic XPG gene."
      Ludwig D.L., Mudgett J.S., Park M.S., Perez-Castro A.V., Macinnes M.A.
      Mamm. Genome 7:644-649(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: DBA/2.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiERCC5_MOUSE
    AccessioniPrimary (citable) accession number: P35689
    Secondary accession number(s): Q61528, Q64248
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 123 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3