Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P35689 (ERCC5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA repair protein complementing XP-G cells homolog

EC=3.1.-.-
Alternative name(s):
DNA excision repair protein ERCC-5
Xeroderma pigmentosum group G-complementing protein homolog
Gene names
Name:Ercc5
Synonyms:Ercc-5, Xpg
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1170 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too By similarity.

Cofactor

Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding By similarity.

Subunit structure

Interacts with PCNA By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11701170DNA repair protein complementing XP-G cells homolog
PRO_0000154032

Regions

Region1 – 9595N-domain
Region752 – 882131I-domain
Motif1049 – 106517Nuclear localization signal Potential

Sites

Metal binding301Magnesium 1 By similarity
Metal binding771Magnesium 1 By similarity
Metal binding7881Magnesium 1 By similarity
Metal binding7901Magnesium 1 By similarity
Metal binding8091Magnesium 2 By similarity
Metal binding8111Magnesium 2 By similarity
Metal binding8601Magnesium 2 By similarity

Amino acid modifications

Modified residue81N6-acetyllysine By similarity
Modified residue3841Phosphoserine Ref.5

Experimental info

Sequence conflict3881C → R in BAA03813. Ref.2
Sequence conflict4191G → R in BAA03813. Ref.2
Sequence conflict4191G → R in AAA91039. Ref.3
Sequence conflict4191G → R in AAB17885. Ref.3
Sequence conflict4621G → E in BAA03813. Ref.2
Sequence conflict4621G → E in AAA91039. Ref.3
Sequence conflict4621G → E in AAB17885. Ref.3
Sequence conflict4881R → S in BAA03813. Ref.2
Sequence conflict6881T → I in BAA03813. Ref.2
Sequence conflict7991T → S in BAA03813. Ref.2
Sequence conflict7991T → S in AAA91039. Ref.3
Sequence conflict7991T → S in AAB17885. Ref.3
Sequence conflict958 – 9592ST → RE in BAA03813. Ref.2
Sequence conflict958 – 9592ST → RE in AAA91039. Ref.3
Sequence conflict958 – 9592ST → RE in AAB17885. Ref.3
Sequence conflict10151N → S in BAA03813. Ref.2
Sequence conflict10211I → M in BAA03813. Ref.2
Sequence conflict11101S → G in BAA03813. Ref.2
Sequence conflict11101S → G in AAA91039. Ref.3
Sequence conflict11101S → G in AAB17885. Ref.3
Sequence conflict11211P → S in BAA03813. Ref.2
Sequence conflict11381D → G in BAA03813. Ref.2
Sequence conflict11381D → G in AAA91039. Ref.3
Sequence conflict11381D → G in AAB17885. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P35689 [UniParc].

Last modified October 3, 2012. Version 4.
Checksum: B4B9F93ACEBC3729

FASTA1,170130,715
        10         20         30         40         50         60 
MGVQGLWKLL ECSGHRVSPE ALEGKVLAVD ISIWLNQALK GVRDSHGNVI ENAHLLTLFH 

        70         80         90        100        110        120 
RLCKLLFFRI RPIFVFDGDA PLLKKQTLAK RRQRKDSASI DSRKTTEKLL KTFLKRQALK 

       130        140        150        160        170        180 
TAFRSSRHEA PPSLTQVQRQ DDIYVLPPLP EEEKHSSEEE DEKQWQARMD QKQALQEEFF 

       190        200        210        220        230        240 
HNPQAIDIES EDFSSLPPEV KHEILTDMKE FTKRRRTLFE AMPEESNDFS QYQLKGLLKK 

       250        260        270        280        290        300 
NYLNQHIENV QKEMNQQHSG QIQRQYQDEG GFLKEVESRR VVSEDTSHYI LIKGIQGKKV 

       310        320        330        340        350        360 
MDVDSESLPS SSNVHSVSSN LKSSPHEKVK PEREPEAAPP SPRTLLAIQA AMLGSSSEDE 

       370        380        390        400        410        420 
PESREGRQSK ERNSGATADA GSISPRTCAA IQKALDDDND EKVSGSSDDL AEKMLLGSGL 

       430        440        450        460        470        480 
EQEEHADETA ERGGGVPFDT APLTPSVTEV KECVTSGSSA NGQTDSAHSF TTASHRCDTP 

       490        500        510        520        530        540 
KETVSLARAV KEASQISSEC EVEGRPAALS PAFIGTPSSH VSGVLSEREP TLAPPTTRTH 

       550        560        570        580        590        600 
SDQGIDIHPE DPELQNGLYP LETKCNSSRL SSDDETEGGQ NPAPKACSTV HVPAEAMSNL 

       610        620        630        640        650        660 
ENALPSNAEE RGDFQETIQL REVPEAAARE LISAPKPMGP MEMESEESES DGSFIEVQSV 

       670        680        690        700        710        720 
VSNSELQTES SEASTHLSEK DAEEPRETLE EGTSRDTECL LQDSSDIEAM EGHREADIDA 

       730        740        750        760        770        780 
EDMPNEWQDI NLEELDALES NLLAEQNSLK AQKQQQDRIA ASVTGQMFLE SQELLRLFGV 

       790        800        810        820        830        840 
PYIQAPMEAE AQCAMLDLTD QTSGTITDDS DIWLFGARHV YKNFFNKNKF VEYYQYVDFY 

       850        860        870        880        890        900 
SQLGLDRNKL INLAYLLGSD YTEGIPTVGC VTAMEILNEF PGRGLDPLLK FSEWWHEAQN 

       910        920        930        940        950        960 
NKKVAENPYD TKVKKKLRKL QLTPGFPNPA VADAYLRPVV DDSRGSFLWG KPDVDKISTF 

       970        980        990       1000       1010       1020 
CQRYFGWNRM KTDESLYPVL KHLNAHQTQL RIDSFFRLAQ QEKQDAKLIK SHRLNRAVTC 

      1030       1040       1050       1060       1070       1080 
ILRKEREEKA PELTKVTEAL DDAKGKTQKR ELPYKKETSV PKRRRPSGNG GFLGDPYCSE 

      1090       1100       1110       1120       1130       1140 
SPQESSCEDG EGSSVMSARQ RSAAESSKIS CSDVPDLVRD PPHGRQGCVS TSSSSEDDED 

      1150       1160       1170 
KAKTVLVTAR PVFGKKKLKL KSMKRRKKKT 

« Hide

References

« Hide 'large scale' references
[1]"An ERCC5 gene with homology to yeast RAD2 is involved in group G Xeroderma pigmentosum."
Shiomi T., Harada Y.-N., Saito T., Shiomi N., Okuno Y., Yamaizumi M.
Mutat. Res. 314:167-175(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Complementary DNA sequence and chromosomal localization of xpg, the mouse counterpart of human repair gene XPG/ERCC5."
Harada Y.N., Matsuda Y., Shiomi N., Shiomi T.
Genomics 28:59-65(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/10.
Tissue: Liver.
[3]"Molecular cloning and structural analysis of the functional mouse genomic XPG gene."
Ludwig D.L., Mudgett J.S., Park M.S., Perez-Castro A.V., Macinnes M.A.
Mamm. Genome 7:644-649(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: DBA/2.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D16306 mRNA. Translation: BAA03813.1.
U40796 mRNA. Translation: AAA91039.1.
U40795 expand/collapse EMBL AC list , U39892, U39893, U39894, U39896, U40073, U40431, U40432, U40668, U40669, U40670, U40792, U40793, U40794 Genomic DNA. Translation: AAB17885.1.
AC123800 Genomic DNA. No translation available.
PIRA57650.
UniGeneMm.2213.

3D structure databases

ProteinModelPortalP35689.
SMRP35689. Positions 2-127, 733-998.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP35689.

Proteomic databases

PRIDEP35689.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:103582. Ercc5.

Phylogenomic databases

eggNOGCOG0258.
HOGENOMHOG000131367.
HOVERGENHBG051501.
InParanoidP35689.

Gene expression databases

GenevestigatorP35689.

Family and domain databases

InterProIPR020045. 5-3_exonuclease_C.
IPR008918. HhH2.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR001044. XPG/Rad2_eukaryotes.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERPTHR11081. PTHR11081. 1 hit.
PfamPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSPR00853. XPGRADSUPER.
PR00066. XRODRMPGMNTG.
SMARTSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMSSF47807. SSF47807. 1 hit.
TIGRFAMsTIGR00600. rad2. 1 hit.
PROSITEPS00841. XPG_1. 1 hit.
PS00842. XPG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP35689.
SOURCESearch...

Entry information

Entry nameERCC5_MOUSE
AccessionPrimary (citable) accession number: P35689
Secondary accession number(s): Q61528, Q64248
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 3, 2012
Last modified: April 16, 2014
This is version 120 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot