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P35689

- ERCC5_MOUSE

UniProt

P35689 - ERCC5_MOUSE

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Protein

DNA repair protein complementing XP-G cells homolog

Gene

Ercc5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301Magnesium 1By similarity
Metal bindingi77 – 771Magnesium 1By similarity
Metal bindingi788 – 7881Magnesium 1By similarity
Metal bindingi790 – 7901Magnesium 1By similarity
Metal bindingi809 – 8091Magnesium 2By similarity
Metal bindingi811 – 8111Magnesium 2By similarity
Metal bindingi860 – 8601Magnesium 2By similarity

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. single-stranded DNA binding Source: InterPro

GO - Biological processi

  1. determination of adult lifespan Source: MGI
  2. DNA repair Source: MGI
  3. multicellular organism growth Source: MGI
  4. nucleotide-excision repair Source: MGI
  5. response to UV Source: MGI
  6. UV protection Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein complementing XP-G cells homolog (EC:3.1.-.-)
Alternative name(s):
DNA excision repair protein ERCC-5
Xeroderma pigmentosum group G-complementing protein homolog
Gene namesi
Name:Ercc5
Synonyms:Ercc-5, Xpg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:103582. Ercc5.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11701170DNA repair protein complementing XP-G cells homologPRO_0000154032Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81N6-acetyllysineBy similarity
Modified residuei384 – 3841Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP35689.
PRIDEiP35689.

PTM databases

PhosphoSiteiP35689.

Expressioni

Gene expression databases

GenevestigatoriP35689.

Interactioni

Subunit structurei

Interacts with PCNA.By similarity

Structurei

3D structure databases

ProteinModelPortaliP35689.
SMRiP35689. Positions 2-113, 733-998.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9595N-domainAdd
BLAST
Regioni752 – 882131I-domainAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1049 – 106517Nuclear localization signalSequence AnalysisAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0258.
HOGENOMiHOG000131367.
HOVERGENiHBG051501.
InParanoidiP35689.

Family and domain databases

Gene3Di3.40.50.1010. 2 hits.
InterProiIPR020045. 5-3_exonuclease_C.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR001044. XPG/Rad2_eukaryotes.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PfamiPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSiPR00066. XRODRMPGMNTG.
SMARTiSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 2 hits.
TIGRFAMsiTIGR00600. rad2. 1 hit.
PROSITEiPS00841. XPG_1. 1 hit.
PS00842. XPG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35689-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGVQGLWKLL ECSGHRVSPE ALEGKVLAVD ISIWLNQALK GVRDSHGNVI
60 70 80 90 100
ENAHLLTLFH RLCKLLFFRI RPIFVFDGDA PLLKKQTLAK RRQRKDSASI
110 120 130 140 150
DSRKTTEKLL KTFLKRQALK TAFRSSRHEA PPSLTQVQRQ DDIYVLPPLP
160 170 180 190 200
EEEKHSSEEE DEKQWQARMD QKQALQEEFF HNPQAIDIES EDFSSLPPEV
210 220 230 240 250
KHEILTDMKE FTKRRRTLFE AMPEESNDFS QYQLKGLLKK NYLNQHIENV
260 270 280 290 300
QKEMNQQHSG QIQRQYQDEG GFLKEVESRR VVSEDTSHYI LIKGIQGKKV
310 320 330 340 350
MDVDSESLPS SSNVHSVSSN LKSSPHEKVK PEREPEAAPP SPRTLLAIQA
360 370 380 390 400
AMLGSSSEDE PESREGRQSK ERNSGATADA GSISPRTCAA IQKALDDDND
410 420 430 440 450
EKVSGSSDDL AEKMLLGSGL EQEEHADETA ERGGGVPFDT APLTPSVTEV
460 470 480 490 500
KECVTSGSSA NGQTDSAHSF TTASHRCDTP KETVSLARAV KEASQISSEC
510 520 530 540 550
EVEGRPAALS PAFIGTPSSH VSGVLSEREP TLAPPTTRTH SDQGIDIHPE
560 570 580 590 600
DPELQNGLYP LETKCNSSRL SSDDETEGGQ NPAPKACSTV HVPAEAMSNL
610 620 630 640 650
ENALPSNAEE RGDFQETIQL REVPEAAARE LISAPKPMGP MEMESEESES
660 670 680 690 700
DGSFIEVQSV VSNSELQTES SEASTHLSEK DAEEPRETLE EGTSRDTECL
710 720 730 740 750
LQDSSDIEAM EGHREADIDA EDMPNEWQDI NLEELDALES NLLAEQNSLK
760 770 780 790 800
AQKQQQDRIA ASVTGQMFLE SQELLRLFGV PYIQAPMEAE AQCAMLDLTD
810 820 830 840 850
QTSGTITDDS DIWLFGARHV YKNFFNKNKF VEYYQYVDFY SQLGLDRNKL
860 870 880 890 900
INLAYLLGSD YTEGIPTVGC VTAMEILNEF PGRGLDPLLK FSEWWHEAQN
910 920 930 940 950
NKKVAENPYD TKVKKKLRKL QLTPGFPNPA VADAYLRPVV DDSRGSFLWG
960 970 980 990 1000
KPDVDKISTF CQRYFGWNRM KTDESLYPVL KHLNAHQTQL RIDSFFRLAQ
1010 1020 1030 1040 1050
QEKQDAKLIK SHRLNRAVTC ILRKEREEKA PELTKVTEAL DDAKGKTQKR
1060 1070 1080 1090 1100
ELPYKKETSV PKRRRPSGNG GFLGDPYCSE SPQESSCEDG EGSSVMSARQ
1110 1120 1130 1140 1150
RSAAESSKIS CSDVPDLVRD PPHGRQGCVS TSSSSEDDED KAKTVLVTAR
1160 1170
PVFGKKKLKL KSMKRRKKKT
Length:1,170
Mass (Da):130,715
Last modified:October 3, 2012 - v4
Checksum:iB4B9F93ACEBC3729
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti388 – 3881C → R in BAA03813. (PubMed:7590748)Curated
Sequence conflicti419 – 4191G → R in BAA03813. (PubMed:7590748)Curated
Sequence conflicti419 – 4191G → R in AAA91039. (PubMed:8703115)Curated
Sequence conflicti419 – 4191G → R in AAB17885. (PubMed:8703115)Curated
Sequence conflicti462 – 4621G → E in BAA03813. (PubMed:7590748)Curated
Sequence conflicti462 – 4621G → E in AAA91039. (PubMed:8703115)Curated
Sequence conflicti462 – 4621G → E in AAB17885. (PubMed:8703115)Curated
Sequence conflicti488 – 4881R → S in BAA03813. (PubMed:7590748)Curated
Sequence conflicti688 – 6881T → I in BAA03813. (PubMed:7590748)Curated
Sequence conflicti799 – 7991T → S in BAA03813. (PubMed:7590748)Curated
Sequence conflicti799 – 7991T → S in AAA91039. (PubMed:8703115)Curated
Sequence conflicti799 – 7991T → S in AAB17885. (PubMed:8703115)Curated
Sequence conflicti958 – 9592ST → RE in BAA03813. (PubMed:7590748)Curated
Sequence conflicti958 – 9592ST → RE in AAA91039. (PubMed:8703115)Curated
Sequence conflicti958 – 9592ST → RE in AAB17885. (PubMed:8703115)Curated
Sequence conflicti1015 – 10151N → S in BAA03813. (PubMed:7590748)Curated
Sequence conflicti1021 – 10211I → M in BAA03813. (PubMed:7590748)Curated
Sequence conflicti1110 – 11101S → G in BAA03813. (PubMed:7590748)Curated
Sequence conflicti1110 – 11101S → G in AAA91039. (PubMed:8703115)Curated
Sequence conflicti1110 – 11101S → G in AAB17885. (PubMed:8703115)Curated
Sequence conflicti1121 – 11211P → S in BAA03813. (PubMed:7590748)Curated
Sequence conflicti1138 – 11381D → G in BAA03813. (PubMed:7590748)Curated
Sequence conflicti1138 – 11381D → G in AAA91039. (PubMed:8703115)Curated
Sequence conflicti1138 – 11381D → G in AAB17885. (PubMed:8703115)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16306 mRNA. Translation: BAA03813.1.
U40796 mRNA. Translation: AAA91039.1.
U40795
, U39892, U39893, U39894, U39896, U40073, U40431, U40432, U40668, U40669, U40670, U40792, U40793, U40794 Genomic DNA. Translation: AAB17885.1.
AC123800 Genomic DNA. No translation available.
CCDSiCCDS35553.1.
PIRiA57650.
UniGeneiMm.2213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16306 mRNA. Translation: BAA03813.1 .
U40796 mRNA. Translation: AAA91039.1 .
U40795
, U39892 , U39893 , U39894 , U39896 , U40073 , U40431 , U40432 , U40668 , U40669 , U40670 , U40792 , U40793 , U40794 Genomic DNA. Translation: AAB17885.1 .
AC123800 Genomic DNA. No translation available.
CCDSi CCDS35553.1.
PIRi A57650.
UniGenei Mm.2213.

3D structure databases

ProteinModelPortali P35689.
SMRi P35689. Positions 2-113, 733-998.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P35689.

Proteomic databases

MaxQBi P35689.
PRIDEi P35689.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:103582. Ercc5.

Phylogenomic databases

eggNOGi COG0258.
HOGENOMi HOG000131367.
HOVERGENi HBG051501.
InParanoidi P35689.

Miscellaneous databases

PROi P35689.
SOURCEi Search...

Gene expression databases

Genevestigatori P35689.

Family and domain databases

Gene3Di 3.40.50.1010. 2 hits.
InterProi IPR020045. 5-3_exonuclease_C.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR001044. XPG/Rad2_eukaryotes.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
[Graphical view ]
Pfami PF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view ]
PRINTSi PR00066. XRODRMPGMNTG.
SMARTi SM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view ]
SUPFAMi SSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 2 hits.
TIGRFAMsi TIGR00600. rad2. 1 hit.
PROSITEi PS00841. XPG_1. 1 hit.
PS00842. XPG_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An ERCC5 gene with homology to yeast RAD2 is involved in group G Xeroderma pigmentosum."
    Shiomi T., Harada Y.-N., Saito T., Shiomi N., Okuno Y., Yamaizumi M.
    Mutat. Res. 314:167-175(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complementary DNA sequence and chromosomal localization of xpg, the mouse counterpart of human repair gene XPG/ERCC5."
    Harada Y.N., Matsuda Y., Shiomi N., Shiomi T.
    Genomics 28:59-65(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/10.
    Tissue: Liver.
  3. "Molecular cloning and structural analysis of the functional mouse genomic XPG gene."
    Ludwig D.L., Mudgett J.S., Park M.S., Perez-Castro A.V., Macinnes M.A.
    Mamm. Genome 7:644-649(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: DBA/2.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiERCC5_MOUSE
AccessioniPrimary (citable) accession number: P35689
Secondary accession number(s): Q61528, Q64248
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 3, 2012
Last modified: November 26, 2014
This is version 125 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3