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Protein

Rho-GTPase-activating protein LRG1

Gene

LRG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts in signal transduction. Activates CDC42, RHO1 and RHO2. Negatively regulates 1,3-beta-glucan synthesis. May be responsible for the down-regulation of CDC42 during mating.2 Publications

GO - Molecular functioni

  • GTPase activator activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cytogamy Source: SGD
  • negative regulation of protein kinase C signaling Source: SGD
  • negative regulation of Rho protein signal transduction Source: SGD
  • positive regulation of GTPase activity Source: SGD
  • regulation of cell wall (1->3)-beta-D-glucan biosynthetic process Source: SGD
  • regulation of fungal-type cell wall organization Source: SGD
  • signal transduction Source: InterPro
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Sporulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29617-MONOMER.
ReactomeiR-SCE-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-GTPase-activating protein LRG1
Alternative name(s):
LIM-RhoGAP protein 1
Gene namesi
Name:LRG1
Ordered Locus Names:YDL240W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL240W.
SGDiS000002399. LRG1.

Subcellular locationi

  • Cytoplasm
  • Bud
  • Bud neck

  • Note: Localized to the bud site during bud formation and at the bud neck during cytokinesis.

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • cytoplasm Source: UniProtKB-SubCell
  • incipient cellular bud site Source: SGD
  • mating projection tip Source: SGD
  • site of polarized growth Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000758381 – 1017Rho-GTPase-activating protein LRG1Add BLAST1017

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei562PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP35688.
PRIDEiP35688.

PTM databases

iPTMnetiP35688.

Expressioni

Developmental stagei

Highly expressed during sporulation.

Interactioni

Subunit structurei

Interacts with CDC42, RHO1 and RHO2.2 Publications

Protein-protein interaction databases

BioGridi31871. 54 interactors.
DIPiDIP-2588N.
IntActiP35688. 4 interactors.
MINTiMINT-422580.

Structurei

3D structure databases

ProteinModelPortaliP35688.
SMRiP35688.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 88LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST61
Domaini98 – 148LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST51
Domaini155 – 184LIM zinc-binding 3; truncatedPROSITE-ProRule annotationAdd BLAST30
Domaini419 – 474LIM zinc-binding 4PROSITE-ProRule annotationAdd BLAST56
Domaini730 – 953Rho-GAPPROSITE-ProRule annotationAdd BLAST224

Sequence similaritiesi

Contains 4 LIM zinc-binding domains.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

GeneTreeiENSGT00760000119123.
HOGENOMiHOG000165645.
InParanoidiP35688.
OMAiKIRIPII.
OrthoDBiEOG092C047S.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.10.110.10. 3 hits.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 2 hits.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 3 hits.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35688-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQNSAGYRS LNTASPMTVQ VKNQKKICAR CNKLVIPDSQ RTKTTLKALG
60 70 80 90 100
KYYHESCFTC QDCQKPLKPK YFPYQVDKTS ESILLCQYDY FRRHNLLCHV
110 120 130 140 150
CDTPLRGLYY TAFGYRYDEE HFSCTICATP CGVKKCFMYG NQLYCKYHFL
160 170 180 190 200
KYFSKRCKGC EFPISDQYIE FPKGEEIHCW HPECYGIHKY WHVNLAAETV
210 220 230 240 250
GLQYLPKLEY NPNSGDKDIN PTAYELDKQM QAFNFILSKT WSVLYRFEEE
260 270 280 290 300
AASCISDMFQ YLTSNDQLKG IESTGLLVLK IDCLFRGLDT LNLSTNKSMP
310 320 330 340 350
VNSDQECIEN NAMAASKYSK FPKNLSTKIM IYLQLLRKLG TENKNETITI
360 370 380 390 400
SSFMSVITGL AHFLKLLTRF GLYTALENNK LTHSVNPLLR FLREVEKNEL
410 420 430 440 450
FENNPFQYIK TPVNATDSCA GCNKYIQEEC IQFYEHRWHI ACFTCSSCHK
460 470 480 490 500
NINPRSLTDP TFNKEKKKIL CSHCSIDDPA SVPGFKFVTK LAQLIFLLKI
510 520 530 540 550
ALVKSRTVML KSKASNKVGR NSLQSTMLKE QTYIRTLNDI KRLRSRRESV
560 570 580 590 600
RVTHNKQQAR KSVILETAET DLNDPTKQGD SKNLVIQTDD PSSSQQVSTR
610 620 630 640 650
ENVFSNTKTL TLDDISRIVA AEQARELRPN AFAHFKKLKE TDDETSNVVP
660 670 680 690 700
KKSGVYYSEL STMELSMIRA ISLSLLAGKQ LISKTDPNYT SLVSMVFSNE
710 720 730 740 750
KQVTGSFWNR MKIMMSMEPK KPITKTVFGA PLDVLCEKWG VDSDLGVGPV
760 770 780 790 800
KIRIPIIIDE LISSLRQMDM SVEGIFRKNG NIRRLRELTA NIDSNPTEAP
810 820 830 840 850
DFSKENAIQL SALLKKFIRE LPQPILSTDL YELWIKAAKI DLEDEKQRVI
860 870 880 890 900
LLIYSLLPTY NRNLLEALLS FLHWTSSFSY IENEMGSKMD IHNLSTVITP
910 920 930 940 950
NILYLRHKEI SNDNVPDEPE SGLVDSFAQN KGENYFLAIE IVDYLITHNE
960 970 980 990 1000
EMAMVPKFLM NLLKDVQLQK LDNYESINHF ISTVMQSKTI DYSECDIKTP
1010
VTVKDSTTTV IQGEINK
Length:1,017
Mass (Da):116,651
Last modified:July 27, 2011 - v3
Checksum:i862EC5EF7E57E679
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti531Q → H in CAA98820 (PubMed:9169867).Curated1
Sequence conflicti766R → S in CAA55210 (PubMed:8065929).Curated1
Sequence conflicti791N → T in CAA55210 (PubMed:8065929).Curated1
Sequence conflicti821L → Q in CAA55210 (PubMed:8065929).Curated1
Sequence conflicti838A → S in CAA55210 (PubMed:8065929).Curated1
Sequence conflicti849V → L in CAA55210 (PubMed:8065929).Curated1
Sequence conflicti895S → F in CAA55210 (PubMed:8065929).Curated1
Sequence conflicti928A → T in CAA55210 (PubMed:8065929).Curated1
Sequence conflicti935Y → S in CAA55210 (PubMed:8065929).Curated1
Sequence conflicti977 – 1017INHFI…GEINK → TILFPPLCKVKQSIIPNVT in CAA55210 (PubMed:8065929).CuratedAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78453 Genomic DNA. Translation: CAA55210.1.
Z74288 Genomic DNA. Translation: CAA98820.1.
BK006938 Genomic DNA. Translation: DAA11626.2.
PIRiS67804.
RefSeqiNP_010041.2. NM_001180300.2.

Genome annotation databases

EnsemblFungiiYDL240W; YDL240W; YDL240W.
GeneIDi851358.
KEGGisce:YDL240W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78453 Genomic DNA. Translation: CAA55210.1.
Z74288 Genomic DNA. Translation: CAA98820.1.
BK006938 Genomic DNA. Translation: DAA11626.2.
PIRiS67804.
RefSeqiNP_010041.2. NM_001180300.2.

3D structure databases

ProteinModelPortaliP35688.
SMRiP35688.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31871. 54 interactors.
DIPiDIP-2588N.
IntActiP35688. 4 interactors.
MINTiMINT-422580.

PTM databases

iPTMnetiP35688.

Proteomic databases

MaxQBiP35688.
PRIDEiP35688.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL240W; YDL240W; YDL240W.
GeneIDi851358.
KEGGisce:YDL240W.

Organism-specific databases

EuPathDBiFungiDB:YDL240W.
SGDiS000002399. LRG1.

Phylogenomic databases

GeneTreeiENSGT00760000119123.
HOGENOMiHOG000165645.
InParanoidiP35688.
OMAiKIRIPII.
OrthoDBiEOG092C047S.

Enzyme and pathway databases

BioCyciYEAST:G3O-29617-MONOMER.
ReactomeiR-SCE-194840. Rho GTPase cycle.

Miscellaneous databases

PROiP35688.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.10.110.10. 3 hits.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 2 hits.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 3 hits.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLRG1_YEAST
AccessioniPrimary (citable) accession number: P35688
Secondary accession number(s): D6VRB6, Q07735
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 159 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 468 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.