P35688 (LRG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rho-GTPase-activating protein LRG1

Alternative name(s):
LIM-RhoGAP protein 1

Gene names

Name:	LRG1
Ordered Locus Names:	YDL240W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1017 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts in signal transduction. Activates CDC42, RHO1 and RHO2. Negatively regulates 1,3-beta-glucan synthesis. May be responsible for the down-regulation of CDC42 during mating. Ref.4 Ref.5
Subunit structure	Interacts with CDC42, RHO1 and RHO2. Ref.4 Ref.5
Subcellular location	Cytoplasm. Bud. Bud neck. Note: Localized to the bud site during bud formation and at the bud neck during cytokinesis. Ref.5 Ref.6
Developmental stage	Highly expressed during sporulation.
Miscellaneous	Present with 468 molecules/cell in log phase SD medium.
Sequence similarities	Contains 4 LIM zinc-binding domains. Contains 1 Rho-GAP domain.

Ontologies

Keywords
Biological process	Sporulation
Cellular component	Cytoplasm
Domain	LIM domain Repeat
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fungal-type cell wall biogenesis Inferred from genetic interaction Ref.5. Source: SGD small GTPase mediated signal transduction Inferred from physical interaction Ref.4. Source: SGD sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cellular bud neck Inferred from direct assay Ref.6. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD
Molecular_function	Rho GTPase activator activity Inferred from direct assay Ref.4. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1017	1017	Rho-GTPase-activating protein LRG1		PRO_0000075838
Regions
	Domain	28 – 88	61	LIM zinc-binding 1
	Domain	98 – 148	51	LIM zinc-binding 2
	Domain	155 – 184	30	LIM zinc-binding 3; truncated
	Domain	419 – 474	56	LIM zinc-binding 4
	Domain	730 – 953	224	Rho-GAP
Amino acid modifications
	Modified residue	562	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	531	1	Q → H in CAA98820. Ref.2
	Sequence conflict	766	1	R → S in CAA55210. Ref.1
	Sequence conflict	791	1	N → T in CAA55210. Ref.1
	Sequence conflict	821	1	L → Q in CAA55210. Ref.1
	Sequence conflict	838	1	A → S in CAA55210. Ref.1
	Sequence conflict	849	1	V → L in CAA55210. Ref.1
	Sequence conflict	895	1	S → F in CAA55210. Ref.1
	Sequence conflict	928	1	A → T in CAA55210. Ref.1
	Sequence conflict	935	1	Y → S in CAA55210. Ref.1
	Sequence conflict	977 – 1017	41	INHFI…GEINK → TILFPPLCKVKQSIIPNVT in CAA55210. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P35688 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: 862EC5EF7E57E679	FASTA	1,017	116,651
	10 20 30 40 50 60 MIQNSAGYRS LNTASPMTVQ VKNQKKICAR CNKLVIPDSQ RTKTTLKALG KYYHESCFTC 70 80 90 100 110 120 QDCQKPLKPK YFPYQVDKTS ESILLCQYDY FRRHNLLCHV CDTPLRGLYY TAFGYRYDEE 130 140 150 160 170 180 HFSCTICATP CGVKKCFMYG NQLYCKYHFL KYFSKRCKGC EFPISDQYIE FPKGEEIHCW 190 200 210 220 230 240 HPECYGIHKY WHVNLAAETV GLQYLPKLEY NPNSGDKDIN PTAYELDKQM QAFNFILSKT 250 260 270 280 290 300 WSVLYRFEEE AASCISDMFQ YLTSNDQLKG IESTGLLVLK IDCLFRGLDT LNLSTNKSMP 310 320 330 340 350 360 VNSDQECIEN NAMAASKYSK FPKNLSTKIM IYLQLLRKLG TENKNETITI SSFMSVITGL 370 380 390 400 410 420 AHFLKLLTRF GLYTALENNK LTHSVNPLLR FLREVEKNEL FENNPFQYIK TPVNATDSCA 430 440 450 460 470 480 GCNKYIQEEC IQFYEHRWHI ACFTCSSCHK NINPRSLTDP TFNKEKKKIL CSHCSIDDPA 490 500 510 520 530 540 SVPGFKFVTK LAQLIFLLKI ALVKSRTVML KSKASNKVGR NSLQSTMLKE QTYIRTLNDI 550 560 570 580 590 600 KRLRSRRESV RVTHNKQQAR KSVILETAET DLNDPTKQGD SKNLVIQTDD PSSSQQVSTR 610 620 630 640 650 660 ENVFSNTKTL TLDDISRIVA AEQARELRPN AFAHFKKLKE TDDETSNVVP KKSGVYYSEL 670 680 690 700 710 720 STMELSMIRA ISLSLLAGKQ LISKTDPNYT SLVSMVFSNE KQVTGSFWNR MKIMMSMEPK 730 740 750 760 770 780 KPITKTVFGA PLDVLCEKWG VDSDLGVGPV KIRIPIIIDE LISSLRQMDM SVEGIFRKNG 790 800 810 820 830 840 NIRRLRELTA NIDSNPTEAP DFSKENAIQL SALLKKFIRE LPQPILSTDL YELWIKAAKI 850 860 870 880 890 900 DLEDEKQRVI LLIYSLLPTY NRNLLEALLS FLHWTSSFSY IENEMGSKMD IHNLSTVITP 910 920 930 940 950 960 NILYLRHKEI SNDNVPDEPE SGLVDSFAQN KGENYFLAIE IVDYLITHNE EMAMVPKFLM 970 980 990 1000 1010 NLLKDVQLQK LDNYESINHF ISTVMQSKTI DYSECDIKTP VTVKDSTTTV IQGEINK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"LRG1 is expressed during sporulation in Saccharomyces cerevisiae and contains motifs similar to LIM and rho/racGAP domains." Mueller A., Xu G., Wells R., Hollenberg C.P., Piepersberg W. Nucleic Acids Res. 22:3151-3154(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DBY874.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 531. Strain: ATCC 204508 / S288c.
[4]	"Functional characterization of the Bag7, Lrg1 and Rgd2 RhoGAP proteins from Saccharomyces cerevisiae." Roumanie O., Weinachter C., Larrieu I., Crouzet M., Doignon F. FEBS Lett. 506:149-156(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDC42; RHO1 AND RHO2.
[5]	"Yeast Lrg1p acts as a specialized RhoGAP regulating 1,3-beta-glucan synthesis." Watanabe D., Abe M., Ohya Y. Yeast 18:943-951(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RHO1, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-562, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78453 Genomic DNA. Translation: CAA55210.1. Z74288 Genomic DNA. Translation: CAA98820.1. BK006938 Genomic DNA. Translation: DAA11626.2.
PIR	S67804.
RefSeq	NP_010041.2. NM_001180300.2.
3D structure databases
ProteinModelPortal	P35688.
SMR	P35688. Positions 28-146, 719-960.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2588N.
IntAct	P35688. 1 interaction.
MINT	MINT-422580.
STRING	4932.YDL240W.
Proteomic databases
PaxDb	P35688.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDL240W; YDL240W; YDL240W.
GeneID	851358.
KEGG	sce:YDL240W.
Organism-specific databases
CYGD	YDL240w.
SGD	S000002399. LRG1.
Phylogenomic databases
eggNOG	NOG267887.
GeneTree	ENSGT00700000104464.
HOGENOM	HOG000165645.
OMA	CISDMLL.
OrthoDB	EOG408RH4.
Enzyme and pathway databases
BioCyc	YEAST:G3O-29617-MONOMER.
Gene expression databases
Genevestigator	P35688.
GermOnline	YDL240W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.555.10. 1 hit. 2.10.110.10. 3 hits.
InterPro	IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP_dom. IPR001781. Znf_LIM. [Graphical view]
Pfam	PF00412. LIM. 3 hits. PF00620. RhoGAP. 1 hit. [Graphical view]
SMART	SM00132. LIM. 3 hits. SM00324. RhoGAP. 1 hit. [Graphical view]
SUPFAM	SSF48350. Rho_GAP. 1 hit.
PROSITE	PS00478. LIM_DOMAIN_1. 2 hits. PS50023. LIM_DOMAIN_2. 3 hits. PS50238. RHOGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968456.

Entry information

Entry name

LRG1_YEAST

Accession

Primary (citable) accession number: P35688
Secondary accession number(s): D6VRB6, Q07735

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	July 27, 2011
Last modified:	May 29, 2013
This is version 128 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program