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P35688

- LRG1_YEAST

UniProt

P35688 - LRG1_YEAST

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Protein

Rho-GTPase-activating protein LRG1

Gene

LRG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts in signal transduction. Activates CDC42, RHO1 and RHO2. Negatively regulates 1,3-beta-glucan synthesis. May be responsible for the down-regulation of CDC42 during mating.2 Publications

GO - Molecular functioni

  1. Rho GTPase activator activity Source: SGD
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. cytogamy Source: SGD
  2. negative regulation of protein kinase C signaling Source: SGD
  3. negative regulation of Rho protein signal transduction Source: SGD
  4. positive regulation of Rho GTPase activity Source: SGD
  5. regulation of cell wall (1->3)-beta-D-glucan biosynthetic process Source: SGD
  6. regulation of fungal-type cell wall organization Source: SGD
  7. signal transduction Source: InterPro
  8. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Sporulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29617-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-GTPase-activating protein LRG1
Alternative name(s):
LIM-RhoGAP protein 1
Gene namesi
Name:LRG1
Ordered Locus Names:YDL240W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL240w.
SGDiS000002399. LRG1.

Subcellular locationi

Cytoplasm. Bud. Bud neck
Note: Localized to the bud site during bud formation and at the bud neck during cytokinesis.

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cellular bud tip Source: SGD
  3. cytoplasm Source: UniProtKB-KW
  4. incipient cellular bud site Source: SGD
  5. mating projection tip Source: SGD
  6. site of polarized growth Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10171017Rho-GTPase-activating protein LRG1PRO_0000075838Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei562 – 5621Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP35688.
PaxDbiP35688.

Expressioni

Developmental stagei

Highly expressed during sporulation.

Gene expression databases

GenevestigatoriP35688.

Interactioni

Subunit structurei

Interacts with CDC42, RHO1 and RHO2.2 Publications

Protein-protein interaction databases

BioGridi31871. 53 interactions.
DIPiDIP-2588N.
IntActiP35688. 4 interactions.
MINTiMINT-422580.
STRINGi4932.YDL240W.

Structurei

3D structure databases

ProteinModelPortaliP35688.
SMRiP35688. Positions 28-146, 719-960.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 8861LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini98 – 14851LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST
Domaini155 – 18430LIM zinc-binding 3; truncatedPROSITE-ProRule annotationAdd
BLAST
Domaini419 – 47456LIM zinc-binding 4PROSITE-ProRule annotationAdd
BLAST
Domaini730 – 953224Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 LIM zinc-binding domains.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiNOG267887.
GeneTreeiENSGT00760000118863.
HOGENOMiHOG000165645.
InParanoidiP35688.
OMAiHTNHFTC.
OrthoDBiEOG73FQVZ.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.10.110.10. 3 hits.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 3 hits.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 3 hits.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35688-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIQNSAGYRS LNTASPMTVQ VKNQKKICAR CNKLVIPDSQ RTKTTLKALG
60 70 80 90 100
KYYHESCFTC QDCQKPLKPK YFPYQVDKTS ESILLCQYDY FRRHNLLCHV
110 120 130 140 150
CDTPLRGLYY TAFGYRYDEE HFSCTICATP CGVKKCFMYG NQLYCKYHFL
160 170 180 190 200
KYFSKRCKGC EFPISDQYIE FPKGEEIHCW HPECYGIHKY WHVNLAAETV
210 220 230 240 250
GLQYLPKLEY NPNSGDKDIN PTAYELDKQM QAFNFILSKT WSVLYRFEEE
260 270 280 290 300
AASCISDMFQ YLTSNDQLKG IESTGLLVLK IDCLFRGLDT LNLSTNKSMP
310 320 330 340 350
VNSDQECIEN NAMAASKYSK FPKNLSTKIM IYLQLLRKLG TENKNETITI
360 370 380 390 400
SSFMSVITGL AHFLKLLTRF GLYTALENNK LTHSVNPLLR FLREVEKNEL
410 420 430 440 450
FENNPFQYIK TPVNATDSCA GCNKYIQEEC IQFYEHRWHI ACFTCSSCHK
460 470 480 490 500
NINPRSLTDP TFNKEKKKIL CSHCSIDDPA SVPGFKFVTK LAQLIFLLKI
510 520 530 540 550
ALVKSRTVML KSKASNKVGR NSLQSTMLKE QTYIRTLNDI KRLRSRRESV
560 570 580 590 600
RVTHNKQQAR KSVILETAET DLNDPTKQGD SKNLVIQTDD PSSSQQVSTR
610 620 630 640 650
ENVFSNTKTL TLDDISRIVA AEQARELRPN AFAHFKKLKE TDDETSNVVP
660 670 680 690 700
KKSGVYYSEL STMELSMIRA ISLSLLAGKQ LISKTDPNYT SLVSMVFSNE
710 720 730 740 750
KQVTGSFWNR MKIMMSMEPK KPITKTVFGA PLDVLCEKWG VDSDLGVGPV
760 770 780 790 800
KIRIPIIIDE LISSLRQMDM SVEGIFRKNG NIRRLRELTA NIDSNPTEAP
810 820 830 840 850
DFSKENAIQL SALLKKFIRE LPQPILSTDL YELWIKAAKI DLEDEKQRVI
860 870 880 890 900
LLIYSLLPTY NRNLLEALLS FLHWTSSFSY IENEMGSKMD IHNLSTVITP
910 920 930 940 950
NILYLRHKEI SNDNVPDEPE SGLVDSFAQN KGENYFLAIE IVDYLITHNE
960 970 980 990 1000
EMAMVPKFLM NLLKDVQLQK LDNYESINHF ISTVMQSKTI DYSECDIKTP
1010
VTVKDSTTTV IQGEINK
Length:1,017
Mass (Da):116,651
Last modified:July 27, 2011 - v3
Checksum:i862EC5EF7E57E679
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti531 – 5311Q → H in CAA98820. (PubMed:9169867)Curated
Sequence conflicti766 – 7661R → S in CAA55210. (PubMed:8065929)Curated
Sequence conflicti791 – 7911N → T in CAA55210. (PubMed:8065929)Curated
Sequence conflicti821 – 8211L → Q in CAA55210. (PubMed:8065929)Curated
Sequence conflicti838 – 8381A → S in CAA55210. (PubMed:8065929)Curated
Sequence conflicti849 – 8491V → L in CAA55210. (PubMed:8065929)Curated
Sequence conflicti895 – 8951S → F in CAA55210. (PubMed:8065929)Curated
Sequence conflicti928 – 9281A → T in CAA55210. (PubMed:8065929)Curated
Sequence conflicti935 – 9351Y → S in CAA55210. (PubMed:8065929)Curated
Sequence conflicti977 – 101741INHFI…GEINK → TILFPPLCKVKQSIIPNVT in CAA55210. (PubMed:8065929)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78453 Genomic DNA. Translation: CAA55210.1.
Z74288 Genomic DNA. Translation: CAA98820.1.
BK006938 Genomic DNA. Translation: DAA11626.2.
PIRiS67804.
RefSeqiNP_010041.2. NM_001180300.2.

Genome annotation databases

EnsemblFungiiYDL240W; YDL240W; YDL240W.
GeneIDi851358.
KEGGisce:YDL240W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78453 Genomic DNA. Translation: CAA55210.1 .
Z74288 Genomic DNA. Translation: CAA98820.1 .
BK006938 Genomic DNA. Translation: DAA11626.2 .
PIRi S67804.
RefSeqi NP_010041.2. NM_001180300.2.

3D structure databases

ProteinModelPortali P35688.
SMRi P35688. Positions 28-146, 719-960.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31871. 53 interactions.
DIPi DIP-2588N.
IntActi P35688. 4 interactions.
MINTi MINT-422580.
STRINGi 4932.YDL240W.

Proteomic databases

MaxQBi P35688.
PaxDbi P35688.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL240W ; YDL240W ; YDL240W .
GeneIDi 851358.
KEGGi sce:YDL240W.

Organism-specific databases

CYGDi YDL240w.
SGDi S000002399. LRG1.

Phylogenomic databases

eggNOGi NOG267887.
GeneTreei ENSGT00760000118863.
HOGENOMi HOG000165645.
InParanoidi P35688.
OMAi HTNHFTC.
OrthoDBi EOG73FQVZ.

Enzyme and pathway databases

BioCyci YEAST:G3O-29617-MONOMER.

Miscellaneous databases

NextBioi 968456.

Gene expression databases

Genevestigatori P35688.

Family and domain databases

Gene3Di 1.10.555.10. 1 hit.
2.10.110.10. 3 hits.
InterProi IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001781. Znf_LIM.
[Graphical view ]
Pfami PF00412. LIM. 3 hits.
PF00620. RhoGAP. 1 hit.
[Graphical view ]
SMARTi SM00132. LIM. 3 hits.
SM00324. RhoGAP. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
PROSITEi PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
PS50238. RHOGAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "LRG1 is expressed during sporulation in Saccharomyces cerevisiae and contains motifs similar to LIM and rho/racGAP domains."
    Mueller A., Xu G., Wells R., Hollenberg C.P., Piepersberg W.
    Nucleic Acids Res. 22:3151-3154(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DBY874.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 531.
    Strain: ATCC 204508 / S288c.
  4. "Functional characterization of the Bag7, Lrg1 and Rgd2 RhoGAP proteins from Saccharomyces cerevisiae."
    Roumanie O., Weinachter C., Larrieu I., Crouzet M., Doignon F.
    FEBS Lett. 506:149-156(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC42; RHO1 AND RHO2.
  5. "Yeast Lrg1p acts as a specialized RhoGAP regulating 1,3-beta-glucan synthesis."
    Watanabe D., Abe M., Ohya Y.
    Yeast 18:943-951(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RHO1, SUBCELLULAR LOCATION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLRG1_YEAST
AccessioniPrimary (citable) accession number: P35688
Secondary accession number(s): D6VRB6, Q07735
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 468 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3