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P35688

- LRG1_YEAST

UniProt

P35688 - LRG1_YEAST

Protein

Rho-GTPase-activating protein LRG1

Gene

LRG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Acts in signal transduction. Activates CDC42, RHO1 and RHO2. Negatively regulates 1,3-beta-glucan synthesis. May be responsible for the down-regulation of CDC42 during mating.2 Publications

    GO - Molecular functioni

    1. Rho GTPase activator activity Source: SGD
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cytogamy Source: SGD
    2. negative regulation of protein kinase C signaling Source: SGD
    3. negative regulation of Rho protein signal transduction Source: SGD
    4. positive regulation of Rho GTPase activity Source: SGD
    5. regulation of cell wall (1->3)-beta-D-glucan biosynthetic process Source: SGD
    6. regulation of fungal-type cell wall organization Source: SGD
    7. signal transduction Source: InterPro
    8. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    GTPase activation

    Keywords - Biological processi

    Sporulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29617-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rho-GTPase-activating protein LRG1
    Alternative name(s):
    LIM-RhoGAP protein 1
    Gene namesi
    Name:LRG1
    Ordered Locus Names:YDL240W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL240w.
    SGDiS000002399. LRG1.

    Subcellular locationi

    Cytoplasm. Bud. Bud neck
    Note: Localized to the bud site during bud formation and at the bud neck during cytokinesis.

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cellular bud tip Source: SGD
    3. cytoplasm Source: UniProtKB-SubCell
    4. incipient cellular bud site Source: SGD
    5. mating projection tip Source: SGD
    6. site of polarized growth Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10171017Rho-GTPase-activating protein LRG1PRO_0000075838Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei562 – 5621Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP35688.
    PaxDbiP35688.

    Expressioni

    Developmental stagei

    Highly expressed during sporulation.

    Gene expression databases

    GenevestigatoriP35688.

    Interactioni

    Subunit structurei

    Interacts with CDC42, RHO1 and RHO2.2 Publications

    Protein-protein interaction databases

    BioGridi31871. 53 interactions.
    DIPiDIP-2588N.
    IntActiP35688. 4 interactions.
    MINTiMINT-422580.
    STRINGi4932.YDL240W.

    Structurei

    3D structure databases

    ProteinModelPortaliP35688.
    SMRiP35688. Positions 28-188, 416-476, 719-960.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 8861LIM zinc-binding 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini98 – 14851LIM zinc-binding 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini155 – 18430LIM zinc-binding 3; truncatedPROSITE-ProRule annotationAdd
    BLAST
    Domaini419 – 47456LIM zinc-binding 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini730 – 953224Rho-GAPPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 4 LIM zinc-binding domains.PROSITE-ProRule annotation
    Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

    Keywords - Domaini

    LIM domain, Repeat

    Phylogenomic databases

    eggNOGiNOG267887.
    GeneTreeiENSGT00740000115221.
    HOGENOMiHOG000165645.
    OMAiHTNHFTC.
    OrthoDBiEOG73FQVZ.

    Family and domain databases

    Gene3Di1.10.555.10. 1 hit.
    2.10.110.10. 3 hits.
    InterProiIPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR001781. Znf_LIM.
    [Graphical view]
    PfamiPF00412. LIM. 3 hits.
    PF00620. RhoGAP. 1 hit.
    [Graphical view]
    SMARTiSM00132. LIM. 3 hits.
    SM00324. RhoGAP. 1 hit.
    [Graphical view]
    SUPFAMiSSF48350. SSF48350. 1 hit.
    PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
    PS50023. LIM_DOMAIN_2. 3 hits.
    PS50238. RHOGAP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35688-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIQNSAGYRS LNTASPMTVQ VKNQKKICAR CNKLVIPDSQ RTKTTLKALG     50
    KYYHESCFTC QDCQKPLKPK YFPYQVDKTS ESILLCQYDY FRRHNLLCHV 100
    CDTPLRGLYY TAFGYRYDEE HFSCTICATP CGVKKCFMYG NQLYCKYHFL 150
    KYFSKRCKGC EFPISDQYIE FPKGEEIHCW HPECYGIHKY WHVNLAAETV 200
    GLQYLPKLEY NPNSGDKDIN PTAYELDKQM QAFNFILSKT WSVLYRFEEE 250
    AASCISDMFQ YLTSNDQLKG IESTGLLVLK IDCLFRGLDT LNLSTNKSMP 300
    VNSDQECIEN NAMAASKYSK FPKNLSTKIM IYLQLLRKLG TENKNETITI 350
    SSFMSVITGL AHFLKLLTRF GLYTALENNK LTHSVNPLLR FLREVEKNEL 400
    FENNPFQYIK TPVNATDSCA GCNKYIQEEC IQFYEHRWHI ACFTCSSCHK 450
    NINPRSLTDP TFNKEKKKIL CSHCSIDDPA SVPGFKFVTK LAQLIFLLKI 500
    ALVKSRTVML KSKASNKVGR NSLQSTMLKE QTYIRTLNDI KRLRSRRESV 550
    RVTHNKQQAR KSVILETAET DLNDPTKQGD SKNLVIQTDD PSSSQQVSTR 600
    ENVFSNTKTL TLDDISRIVA AEQARELRPN AFAHFKKLKE TDDETSNVVP 650
    KKSGVYYSEL STMELSMIRA ISLSLLAGKQ LISKTDPNYT SLVSMVFSNE 700
    KQVTGSFWNR MKIMMSMEPK KPITKTVFGA PLDVLCEKWG VDSDLGVGPV 750
    KIRIPIIIDE LISSLRQMDM SVEGIFRKNG NIRRLRELTA NIDSNPTEAP 800
    DFSKENAIQL SALLKKFIRE LPQPILSTDL YELWIKAAKI DLEDEKQRVI 850
    LLIYSLLPTY NRNLLEALLS FLHWTSSFSY IENEMGSKMD IHNLSTVITP 900
    NILYLRHKEI SNDNVPDEPE SGLVDSFAQN KGENYFLAIE IVDYLITHNE 950
    EMAMVPKFLM NLLKDVQLQK LDNYESINHF ISTVMQSKTI DYSECDIKTP 1000
    VTVKDSTTTV IQGEINK 1017
    Length:1,017
    Mass (Da):116,651
    Last modified:July 27, 2011 - v3
    Checksum:i862EC5EF7E57E679
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti531 – 5311Q → H in CAA98820. (PubMed:9169867)Curated
    Sequence conflicti766 – 7661R → S in CAA55210. (PubMed:8065929)Curated
    Sequence conflicti791 – 7911N → T in CAA55210. (PubMed:8065929)Curated
    Sequence conflicti821 – 8211L → Q in CAA55210. (PubMed:8065929)Curated
    Sequence conflicti838 – 8381A → S in CAA55210. (PubMed:8065929)Curated
    Sequence conflicti849 – 8491V → L in CAA55210. (PubMed:8065929)Curated
    Sequence conflicti895 – 8951S → F in CAA55210. (PubMed:8065929)Curated
    Sequence conflicti928 – 9281A → T in CAA55210. (PubMed:8065929)Curated
    Sequence conflicti935 – 9351Y → S in CAA55210. (PubMed:8065929)Curated
    Sequence conflicti977 – 101741INHFI…GEINK → TILFPPLCKVKQSIIPNVT in CAA55210. (PubMed:8065929)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78453 Genomic DNA. Translation: CAA55210.1.
    Z74288 Genomic DNA. Translation: CAA98820.1.
    BK006938 Genomic DNA. Translation: DAA11626.2.
    PIRiS67804.
    RefSeqiNP_010041.2. NM_001180300.2.

    Genome annotation databases

    EnsemblFungiiYDL240W; YDL240W; YDL240W.
    GeneIDi851358.
    KEGGisce:YDL240W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78453 Genomic DNA. Translation: CAA55210.1 .
    Z74288 Genomic DNA. Translation: CAA98820.1 .
    BK006938 Genomic DNA. Translation: DAA11626.2 .
    PIRi S67804.
    RefSeqi NP_010041.2. NM_001180300.2.

    3D structure databases

    ProteinModelPortali P35688.
    SMRi P35688. Positions 28-188, 416-476, 719-960.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31871. 53 interactions.
    DIPi DIP-2588N.
    IntActi P35688. 4 interactions.
    MINTi MINT-422580.
    STRINGi 4932.YDL240W.

    Proteomic databases

    MaxQBi P35688.
    PaxDbi P35688.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL240W ; YDL240W ; YDL240W .
    GeneIDi 851358.
    KEGGi sce:YDL240W.

    Organism-specific databases

    CYGDi YDL240w.
    SGDi S000002399. LRG1.

    Phylogenomic databases

    eggNOGi NOG267887.
    GeneTreei ENSGT00740000115221.
    HOGENOMi HOG000165645.
    OMAi HTNHFTC.
    OrthoDBi EOG73FQVZ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29617-MONOMER.

    Miscellaneous databases

    NextBioi 968456.

    Gene expression databases

    Genevestigatori P35688.

    Family and domain databases

    Gene3Di 1.10.555.10. 1 hit.
    2.10.110.10. 3 hits.
    InterProi IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR001781. Znf_LIM.
    [Graphical view ]
    Pfami PF00412. LIM. 3 hits.
    PF00620. RhoGAP. 1 hit.
    [Graphical view ]
    SMARTi SM00132. LIM. 3 hits.
    SM00324. RhoGAP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48350. SSF48350. 1 hit.
    PROSITEi PS00478. LIM_DOMAIN_1. 2 hits.
    PS50023. LIM_DOMAIN_2. 3 hits.
    PS50238. RHOGAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "LRG1 is expressed during sporulation in Saccharomyces cerevisiae and contains motifs similar to LIM and rho/racGAP domains."
      Mueller A., Xu G., Wells R., Hollenberg C.P., Piepersberg W.
      Nucleic Acids Res. 22:3151-3154(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DBY874.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 531.
      Strain: ATCC 204508 / S288c.
    4. "Functional characterization of the Bag7, Lrg1 and Rgd2 RhoGAP proteins from Saccharomyces cerevisiae."
      Roumanie O., Weinachter C., Larrieu I., Crouzet M., Doignon F.
      FEBS Lett. 506:149-156(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC42; RHO1 AND RHO2.
    5. "Yeast Lrg1p acts as a specialized RhoGAP regulating 1,3-beta-glucan synthesis."
      Watanabe D., Abe M., Ohya Y.
      Yeast 18:943-951(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RHO1, SUBCELLULAR LOCATION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLRG1_YEAST
    AccessioniPrimary (citable) accession number: P35688
    Secondary accession number(s): D6VRB6, Q07735
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 140 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 468 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3