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P35677

- T2N5_NOSS7

UniProt

P35677 - T2N5_NOSS7

Protein

Type-2 restriction enzyme NspV

Gene

nspVR

Organism
Nostoc sp. (strain ATCC 29411 / PCC 7524)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 2 (29 May 2013)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence TTCGAA and cleaves after T-2.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi57743. Nsp7524ORF1224P.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme NspV (EC:3.1.21.4)
    Short name:
    R.NspV
    Alternative name(s):
    Endonuclease NspV
    Type II restriction enzyme NspV
    Gene namesi
    Name:nspVR
    Ordered Locus Names:Nos7524_1225
    OrganismiNostoc sp. (strain ATCC 29411 / PCC 7524)
    Taxonomic identifieri28072 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
    ProteomesiUP000010378: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 220220Type-2 restriction enzyme NspVPRO_0000077352Add
    BLAST

    Sequencei

    Sequence statusi: Complete.

    P35677-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTILTIEALR TEAAIFSAAE SIHPEPLLYG VTDGKAVGTY IEQKFRLYLK    50
    EHYEFVQGNS ASGIDFPGLL VDVKVTSIRQ PQSSCPFKSA RQKIFGLGYS 100
    LLIFVYDKID NSTNRTATLN ILHTIYVSAE RTADFQMTRG IRNILANEGN 150
    KDDLIAFMSD RNLPVDEIEA GNVADEILRN PPMQGFLTIS NALQWRLQYG 200
    RVIERAGQED GILTVYRNNP 220
    Length:220
    Mass (Da):24,664
    Last modified:May 29, 2013 - v2
    Checksum:iD788B9605C0ED16B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti175 – 1773DEI → AIL in BAA03538. (PubMed:8367311)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14719 Genomic DNA. Translation: BAA03538.1.
    CP003552 Genomic DNA. Translation: AFY47112.1.
    RefSeqiWP_015137568.1. NC_019684.1.
    YP_007074709.1. NC_019684.1.

    Genome annotation databases

    EnsemblBacteriaiAFY47112; AFY47112; Nos7524_1225.
    GeneIDi14089186.
    KEGGinop:Nos7524_1225.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14719 Genomic DNA. Translation: BAA03538.1 .
    CP003552 Genomic DNA. Translation: AFY47112.1 .
    RefSeqi WP_015137568.1. NC_019684.1.
    YP_007074709.1. NC_019684.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 57743. Nsp7524ORF1224P.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AFY47112 ; AFY47112 ; Nos7524_1225 .
    GeneIDi 14089186.
    KEGGi nop:Nos7524_1225.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the NspV restriction-modification genes of Nostoc sp. strain PCC7524."
      Ueno T., Ito H., Kotani H., Nakajima K.
      Nucleic Acids Res. 21:3899-3899(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 29411 / PCC 7524.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29411 / PCC 7524.

    Entry informationi

    Entry nameiT2N5_NOSS7
    AccessioniPrimary (citable) accession number: P35677
    Secondary accession number(s): K9QQL2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: May 29, 2013
    Last modified: October 1, 2014
    This is version 48 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3