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P35677 (T2N5_NOSS7) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme NspV

Short name=R.NspV
EC=3.1.21.4
Alternative name(s):
Endonuclease NspV
Type II restriction enzyme NspV
Gene names
Name:nspVR
OrganismNostoc sp. (strain ATCC 29411 / PCC 7524)
Taxonomic identifier28072 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length220 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence TTCGAA and cleaves after T-2.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 220220Type-2 restriction enzyme NspV
PRO_0000077352

Sequences

Sequence LengthMass (Da)Tools
P35677 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: BA38B9644794B8C6

FASTA22024,604
        10         20         30         40         50         60 
MTILTIEALR TEAAIFSAAE SIHPEPLLYG VTDGKAVGTY IEQKFRLYLK EHYEFVQGNS 

        70         80         90        100        110        120 
ASGIDFPGLL VDVKVTSIRQ PQSSCPFKSA RQKIFGLGYS LLIFVYDKID NSTNRTATLN 

       130        140        150        160        170        180 
ILHTIYVSAE RTADFQMTRG IRNILANEGN KDDLIAFMSD RNLPVDEIEA GNVAAILLRN 

       190        200        210        220 
PPMQGFLTIS NALQWRLQYG RVIERAGQED GILTVYRNNP 

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References

[1]"Cloning and expression of the NspV restriction-modification genes of Nostoc sp. strain PCC7524."
Ueno T., Ito H., Kotani H., Nakajima K.
Nucleic Acids Res. 21:3899-3899(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D14719 Genomic DNA. Translation: BAA03538.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

REBASE1407. NspV.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2N5_NOSS7
AccessionPrimary (citable) accession number: P35677
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 3, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries