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P35677

- T2N5_NOSS7

UniProt

P35677 - T2N5_NOSS7

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Protein

Type-2 restriction enzyme NspV

Gene

nspVR

Organism
Nostoc sp. (strain ATCC 29411 / PCC 7524)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi

Functioni

Recognizes the double-stranded sequence TTCGAA and cleaves after T-2.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi57743. Nsp7524ORF1224P.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme NspV (EC:3.1.21.4)
Short name:
R.NspV
Alternative name(s):
Endonuclease NspV
Type II restriction enzyme NspV
Gene namesi
Name:nspVR
Ordered Locus Names:Nos7524_1225
OrganismiNostoc sp. (strain ATCC 29411 / PCC 7524)
Taxonomic identifieri28072 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000010378: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Type-2 restriction enzyme NspVPRO_0000077352Add
BLAST

Sequencei

Sequence statusi: Complete.

P35677 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MTILTIEALR TEAAIFSAAE SIHPEPLLYG VTDGKAVGTY IEQKFRLYLK
60 70 80 90 100
EHYEFVQGNS ASGIDFPGLL VDVKVTSIRQ PQSSCPFKSA RQKIFGLGYS
110 120 130 140 150
LLIFVYDKID NSTNRTATLN ILHTIYVSAE RTADFQMTRG IRNILANEGN
160 170 180 190 200
KDDLIAFMSD RNLPVDEIEA GNVADEILRN PPMQGFLTIS NALQWRLQYG
210 220
RVIERAGQED GILTVYRNNP
Length:220
Mass (Da):24,664
Last modified:May 29, 2013 - v2
Checksum:iD788B9605C0ED16B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti175 – 1773DEI → AIL in BAA03538. (PubMed:8367311)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14719 Genomic DNA. Translation: BAA03538.1.
CP003552 Genomic DNA. Translation: AFY47112.1.
RefSeqiWP_015137568.1. NC_019684.1.
YP_007074709.1. NC_019684.1.

Genome annotation databases

EnsemblBacteriaiAFY47112; AFY47112; Nos7524_1225.
GeneIDi14089186.
KEGGinop:Nos7524_1225.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14719 Genomic DNA. Translation: BAA03538.1 .
CP003552 Genomic DNA. Translation: AFY47112.1 .
RefSeqi WP_015137568.1. NC_019684.1.
YP_007074709.1. NC_019684.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 57743. Nsp7524ORF1224P.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AFY47112 ; AFY47112 ; Nos7524_1225 .
GeneIDi 14089186.
KEGGi nop:Nos7524_1225.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the NspV restriction-modification genes of Nostoc sp. strain PCC7524."
    Ueno T., Ito H., Kotani H., Nakajima K.
    Nucleic Acids Res. 21:3899-3899(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 29411 / PCC 7524.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29411 / PCC 7524.

Entry informationi

Entry nameiT2N5_NOSS7
AccessioniPrimary (citable) accession number: P35677
Secondary accession number(s): K9QQL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: May 29, 2013
Last modified: October 1, 2014
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3