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P35677

- T2N5_NOSS7

UniProt

P35677 - T2N5_NOSS7

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Protein
Type-2 restriction enzyme NspV
Gene
nspVR, Nos7524_1225
Organism
Nostoc sp. (strain ATCC 29411 / PCC 7524)
Status
Reviewed - Annotation score: 2 out of 5 - Protein predictedi

Functioni

Recognizes the double-stranded sequence TTCGAA and cleaves after T-2.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi57743. Nsp7524ORF1224P.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme NspV (EC:3.1.21.4)
Short name:
R.NspV
Alternative name(s):
Endonuclease NspV
Type II restriction enzyme NspV
Gene namesi
Name:nspVR
Ordered Locus Names:Nos7524_1225
OrganismiNostoc sp. (strain ATCC 29411 / PCC 7524)
Taxonomic identifieri28072 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000010378: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Type-2 restriction enzyme NspV
PRO_0000077352Add
BLAST

Sequencei

Sequence statusi: Complete.

P35677-1 [UniParc]FASTAAdd to Basket

« Hide

MTILTIEALR TEAAIFSAAE SIHPEPLLYG VTDGKAVGTY IEQKFRLYLK    50
EHYEFVQGNS ASGIDFPGLL VDVKVTSIRQ PQSSCPFKSA RQKIFGLGYS 100
LLIFVYDKID NSTNRTATLN ILHTIYVSAE RTADFQMTRG IRNILANEGN 150
KDDLIAFMSD RNLPVDEIEA GNVADEILRN PPMQGFLTIS NALQWRLQYG 200
RVIERAGQED GILTVYRNNP 220
Length:220
Mass (Da):24,664
Last modified:May 29, 2013 - v2
Checksum:iD788B9605C0ED16B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti175 – 1773DEI → AIL in BAA03538. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14719 Genomic DNA. Translation: BAA03538.1.
CP003552 Genomic DNA. Translation: AFY47112.1.
RefSeqiWP_015137568.1. NC_019684.1.
YP_007074709.1. NC_019684.1.

Genome annotation databases

EnsemblBacteriaiAFY47112; AFY47112; Nos7524_1225.
GeneIDi14089186.
KEGGinop:Nos7524_1225.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14719 Genomic DNA. Translation: BAA03538.1 .
CP003552 Genomic DNA. Translation: AFY47112.1 .
RefSeqi WP_015137568.1. NC_019684.1.
YP_007074709.1. NC_019684.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 57743. Nsp7524ORF1224P.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AFY47112 ; AFY47112 ; Nos7524_1225 .
GeneIDi 14089186.
KEGGi nop:Nos7524_1225.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the NspV restriction-modification genes of Nostoc sp. strain PCC7524."
    Ueno T., Ito H., Kotani H., Nakajima K.
    Nucleic Acids Res. 21:3899-3899(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 29411 / PCC 7524.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29411 / PCC 7524.

Entry informationi

Entry nameiT2N5_NOSS7
AccessioniPrimary (citable) accession number: P35677
Secondary accession number(s): K9QQL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: May 29, 2013
Last modified: September 3, 2014
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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