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Reviewed, UniProtKB/Swiss-Prot P35677 (T2N5_NOSS7)

Last modified November 3, 2009. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Type-2 restriction enzyme NspV
      Short name=R.NspV
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme NspV
    Endonuclease NspV
Gene names
Name: nspVR
OrganismNostoc sp. (strain PCC 7524)
Taxonomic identifier28072 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length220 AA.
Sequence statusComplete.
Protein existencePredicted.

General annotation (Comments)

Function

Recognizes the double-stranded sequence TTCGAA and cleaves after T-2.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 220220Type-2 restriction enzyme NspV
PRO_0000077352

Sequences

Sequence LengthMass (Da)Tools
P35677-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: BA38B9644794B8C6

FASTA22024,604
        10         20         30         40         50         60 
MTILTIEALR TEAAIFSAAE SIHPEPLLYG VTDGKAVGTY IEQKFRLYLK EHYEFVQGNS 

        70         80         90        100        110        120 
ASGIDFPGLL VDVKVTSIRQ PQSSCPFKSA RQKIFGLGYS LLIFVYDKID NSTNRTATLN 

       130        140        150        160        170        180 
ILHTIYVSAE RTADFQMTRG IRNILANEGN KDDLIAFMSD RNLPVDEIEA GNVAAILLRN 

       190        200        210        220 
PPMQGFLTIS NALQWRLQYG RVIERAGQED GILTVYRNNP 

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References

[1]"Cloning and expression of the NspV restriction-modification genes of Nostoc sp. strain PCC7524."
Ueno T., Ito H., Kotani H., Nakajima K.
Nucleic Acids Res. 21:3899-3899(1993) [PubMed: 8367311] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D14719 Genomic DNA. Translation: BAA03538.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

REBASE1407. NspV.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2N5_NOSS7
AccessionPrimary (citable) accession number: P35677
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents