Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P35677 (T2N5_NOSS7) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme NspV

Short name=R.NspV
EC=3.1.21.4
Alternative name(s):
Endonuclease NspV
Type II restriction enzyme NspV
Gene names
Name:nspVR
Ordered Locus Names:Nos7524_1225
OrganismNostoc sp. (strain ATCC 29411 / PCC 7524) [Complete proteome] [HAMAP]
Taxonomic identifier28072 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length220 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence TTCGAA and cleaves after T-2.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 220220Type-2 restriction enzyme NspV
PRO_0000077352

Experimental info

Sequence conflict175 – 1773DEI → AIL in BAA03538. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P35677 [UniParc].

Last modified May 29, 2013. Version 2.
Checksum: D788B9605C0ED16B

FASTA22024,664
        10         20         30         40         50         60 
MTILTIEALR TEAAIFSAAE SIHPEPLLYG VTDGKAVGTY IEQKFRLYLK EHYEFVQGNS 

        70         80         90        100        110        120 
ASGIDFPGLL VDVKVTSIRQ PQSSCPFKSA RQKIFGLGYS LLIFVYDKID NSTNRTATLN 

       130        140        150        160        170        180 
ILHTIYVSAE RTADFQMTRG IRNILANEGN KDDLIAFMSD RNLPVDEIEA GNVADEILRN 

       190        200        210        220 
PPMQGFLTIS NALQWRLQYG RVIERAGQED GILTVYRNNP 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of the NspV restriction-modification genes of Nostoc sp. strain PCC7524."
Ueno T., Ito H., Kotani H., Nakajima K.
Nucleic Acids Res. 21:3899-3899(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 29411 / PCC 7524.
[2]"Improving the coverage of the cyanobacterial phylum using diversity-driven genome sequencing."
Shih P.M., Wu D., Latifi A., Axen S.D., Fewer D.P., Talla E., Calteau A., Cai F., Tandeau de Marsac N., Rippka R., Herdman M., Sivonen K., Coursin T., Laurent T., Goodwin L., Nolan M., Davenport K.W., Han C.S. expand/collapse author list , Rubin E.M., Eisen J.A., Woyke T., Gugger M., Kerfeld C.A.
Proc. Natl. Acad. Sci. U.S.A. 110:1053-1058(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29411 / PCC 7524.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D14719 Genomic DNA. Translation: BAA03538.1.
CP003552 Genomic DNA. Translation: AFY47112.1.
RefSeqYP_007074709.1. NC_019684.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE57743. Nsp7524ORF1224P.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFY47112; AFY47112; Nos7524_1225.
GeneID14089186.
KEGGnop:Nos7524_1225.

Organism-specific databases

CMRSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2N5_NOSS7
AccessionPrimary (citable) accession number: P35677
Secondary accession number(s): K9QQL2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: May 29, 2013
Last modified: April 16, 2014
This is version 46 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries