Reviewed, UniProtKB/Swiss-Prot P35677 (T2N5_NOSS7)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme NspV
      Short name=R.NspV
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme NspV
    Endonuclease NspV

Gene names

Name:

nspVR

Organism

Nostoc sp. (strain PCC 7524)

Taxonomic identifier

28072 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence TTCGAA and cleaves after T-2.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Type-2 restriction enzyme NspV		PRO_0000077352

Sequences

	Sequence		Length	Mass (Da)
	P35677-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: BA38B9644794B8C6	FASTA	220	24,604
	10 20 30 40 50 60 MTILTIEALR TEAAIFSAAE SIHPEPLLYG VTDGKAVGTY IEQKFRLYLK EHYEFVQGNS 70 80 90 100 110 120 ASGIDFPGLL VDVKVTSIRQ PQSSCPFKSA RQKIFGLGYS LLIFVYDKID NSTNRTATLN 130 140 150 160 170 180 ILHTIYVSAE RTADFQMTRG IRNILANEGN KDDLIAFMSD RNLPVDEIEA GNVAAILLRN 190 200 210 220 PPMQGFLTIS NALQWRLQYG RVIERAGQED GILTVYRNNP

References

[1]	"Cloning and expression of the NspV restriction-modification genes of Nostoc sp. strain PCC7524." Ueno T., Ito H., Kotani H., Nakajima K. Nucleic Acids Res. 21:3899-3899(1993) [PubMed: 8367311] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D14719 Genomic DNA. Translation: BAA03538.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1407. NspV.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2N5_NOSS7

Accession

Primary (citable) accession number: P35677

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)