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P35669

- GSHB_SCHPO

UniProt

P35669 - GSHB_SCHPO

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Protein
Glutathione synthetase large chain
Gene
gsa1, gsh2, SPAC3F10.04
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei128 – 1281Substrate By similarity
Metal bindingi146 – 1461Magnesium By similarity
Binding sitei146 – 1461ATP By similarity
Metal bindingi148 – 1481Magnesium By similarity
Binding sitei239 – 2391Substrate By similarity
Binding sitei330 – 3301ATP By similarity
Metal bindingi391 – 3911Magnesium By similarity
Binding sitei398 – 3981ATP By similarity
Binding sitei446 – 4461ATP By similarity
Binding sitei473 – 4731Substrate By similarity
Binding sitei475 – 4751ATP By similarity
Binding sitei481 – 4811ATP; via carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi387 – 39610ATP By similarity
Nucleotide bindingi420 – 4234ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. glutathione binding Source: UniProtKB
  3. glutathione synthase activity Source: PomBase
  4. magnesium ion binding Source: UniProtKB
  5. protein homodimerization activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. cellular response to cadmium ion Source: PomBase
  2. detoxification of cadmium ion Source: PomBase
  3. glutathione biosynthetic process Source: PomBase
  4. glutathione transport Source: PomBase
  5. phytochelatin biosynthetic process Source: PomBase
  6. phytochelatin-metal complex formation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10022.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase large chain (EC:6.3.2.3)
Short name:
GSH synthetase large chain
Short name:
GSH-S
Alternative name(s):
Glutathione synthase large chain
Phytochelatin synthetase
Gene namesi
Name:gsa1
Synonyms:gsh2
ORF Names:SPAC3F10.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC3F10.04.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: PomBase
  2. glutathione synthase complex Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Glutathione synthetase large chain
PRO_0000211265Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei356 – 3561Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35669.

Interactioni

Subunit structurei

Heterodimer composed of a large and a small chain.

Protein-protein interaction databases

BioGridi279340. 8 interactions.
MINTiMINT-4688612.
STRINGi4896.SPAC3F10.04-1.

Structurei

3D structure databases

ProteinModelPortaliP35669.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1534Substrate binding By similarity
Regioni233 – 2353Substrate binding By similarity
Regioni291 – 2944Substrate binding By similarity
Regioni484 – 4852Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG329040.
HOGENOMiHOG000172641.
KOiK01920.
OMAiEGRNIAT.
OrthoDBiEOG7GBG71.
PhylomeDBiP35669.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.30.470.20. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P35669-1 [UniParc]FASTAAdd to Basket

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MEIEKYTPEQ IEELGKGARD FAFAHGVVFT ELSVSKEGRN IATQIPITLF    50
PSVIPHGAFV EAVSVQKAYN KLYAKIANDY EFLRLHLQSI TKYDEFMNKL 100
WNLYQKHREA VAHLKENQFQ PLSLGVFRSD YMVHQDDSFI GCKQVEFNTI 150
SVSFGGVSKA VSNLHAYCSQ SGLYRKPLTT NYLTVNTSVS GICTGISNAV 200
DAYRDYVKNI TSKMNIASDN TKPIVLFVVK GGERNITDQR TLEYELLNRF 250
HVISKRIDIA ELNSLIHDKS SNKLYMKTSF TTYEVAVVYY RVGYALDDYP 300
SQEAWDMRLT IENTLAIKCP SISTHLAGSK KIQQVLAESN ALERFLEGDE 350
LQAVRSTFAD MYPLDDTPRG KEGIKLAFEK PEDFVLKPQR EGGGNNTYGK 400
DIPGLLSKMP QEEWDSYILM RYINAVPSQN YILKGERPEK FDVVDEIGIL 450
GTIVWNIKTD EVVQNGQSGF ICRTKPKKTN EGGVATGYAS LSSIELSE 498
Length:498
Mass (Da):56,153
Last modified:October 1, 1996 - v2
Checksum:iAD2921C5AD667ABF
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti132 – 1321M → T in AAL41009. 1 Publication
Sequence conflicti263 – 2631N → T in AAA35307. 1 Publication
Sequence conflicti304 – 3041A → R in AAA35307. 1 Publication
Sequence conflicti330 – 3301K → E in AAA35307. 1 Publication
Sequence conflicti458 – 4581K → N in AAA35307. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAA93302.1.
AF448236 Genomic DNA. Translation: AAL41009.1.
Y08414 Genomic DNA. Translation: CAA69691.1.
M85179 Genomic DNA. Translation: AAA35307.1.
PIRiJT0961.
T38705.
RefSeqiNP_593936.1. NM_001019364.2.

Genome annotation databases

EnsemblFungiiSPAC3F10.04.1; SPAC3F10.04.1:pep; SPAC3F10.04.
GeneIDi2542896.
KEGGispo:SPAC3F10.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAA93302.1 .
AF448236 Genomic DNA. Translation: AAL41009.1 .
Y08414 Genomic DNA. Translation: CAA69691.1 .
M85179 Genomic DNA. Translation: AAA35307.1 .
PIRi JT0961.
T38705.
RefSeqi NP_593936.1. NM_001019364.2.

3D structure databases

ProteinModelPortali P35669.
ModBasei Search...

Protein-protein interaction databases

BioGridi 279340. 8 interactions.
MINTi MINT-4688612.
STRINGi 4896.SPAC3F10.04-1.

Proteomic databases

MaxQBi P35669.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC3F10.04.1 ; SPAC3F10.04.1:pep ; SPAC3F10.04 .
GeneIDi 2542896.
KEGGi spo:SPAC3F10.04.

Organism-specific databases

PomBasei SPAC3F10.04.

Phylogenomic databases

eggNOGi NOG329040.
HOGENOMi HOG000172641.
KOi K01920.
OMAi EGRNIAT.
OrthoDBi EOG7GBG71.
PhylomeDBi P35669.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00210 .
BioCyci MetaCyc:MONOMER-10022.

Miscellaneous databases

NextBioi 20803935.
PROi P35669.

Family and domain databases

Gene3Di 1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.30.470.20. 1 hit.
3.40.50.1760. 1 hit.
InterProi IPR013816. ATP_grasp_subdomain_2.
IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
PANTHERi PTHR11130. PTHR11130. 1 hit.
Pfami PF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001558. GSHase. 1 hit.
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Biosynthesis of phytochelatins in the fission yeast. Phytochelatin synthesis: a second role for the glutathione synthetase gene of Schizosaccharomyces pombe."
    Al-Lahham A., Rohde V., Heim P., Leuchter R., Veeck J., Wunderlich C., Wolf K., Zimmermann M.
    Yeast 15:385-396(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SP011.
  2. Shin Y.H., Lim C.-J.
    Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "Cloning and sequencing of the gene encoding the large subunit of glutathione synthetase of Schizosaccharomyces pombe."
    Mutoh N., Nakagawa C.W., Ando S., Tanabe K., Hayashi Y.
    Biochem. Biophys. Res. Commun. 181:430-436(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 214-498, PROTEIN SEQUENCE OF 218-229 AND 392-403.
    Strain: 972 / ATCC 24843.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-356, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiGSHB_SCHPO
AccessioniPrimary (citable) accession number: P35669
Secondary accession number(s): Q8X1A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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