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P35669

- GSHB_SCHPO

UniProt

P35669 - GSHB_SCHPO

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Protein

Glutathione synthetase large chain

Gene

gsa1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei128 – 1281SubstrateBy similarity
Metal bindingi146 – 1461MagnesiumBy similarity
Binding sitei146 – 1461ATPBy similarity
Metal bindingi148 – 1481MagnesiumBy similarity
Binding sitei239 – 2391SubstrateBy similarity
Binding sitei330 – 3301ATPBy similarity
Metal bindingi391 – 3911MagnesiumBy similarity
Binding sitei398 – 3981ATPBy similarity
Binding sitei446 – 4461ATPBy similarity
Binding sitei473 – 4731SubstrateBy similarity
Binding sitei475 – 4751ATPBy similarity
Binding sitei481 – 4811ATP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi387 – 39610ATPBy similarity
Nucleotide bindingi420 – 4234ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. glutathione binding Source: UniProtKB
  3. glutathione synthase activity Source: PomBase
  4. magnesium ion binding Source: UniProtKB
  5. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. cellular response to cadmium ion Source: PomBase
  2. detoxification of cadmium ion Source: PomBase
  3. glutathione biosynthetic process Source: PomBase
  4. glutathione transport Source: PomBase
  5. phytochelatin biosynthetic process Source: PomBase
  6. phytochelatin-metal complex formation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10022.
ReactomeiREACT_255388. Glutathione synthesis and recycling.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase large chain (EC:6.3.2.3)
Short name:
GSH synthetase large chain
Short name:
GSH-S
Alternative name(s):
Glutathione synthase large chain
Phytochelatin synthetase
Gene namesi
Name:gsa1
Synonyms:gsh2
ORF Names:SPAC3F10.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC3F10.04.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: PomBase
  2. glutathione synthase complex Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Glutathione synthetase large chainPRO_0000211265Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei356 – 3561Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35669.

Interactioni

Subunit structurei

Heterodimer composed of a large and a small chain.

Protein-protein interaction databases

BioGridi279340. 8 interactions.
MINTiMINT-4688612.
STRINGi4896.SPAC3F10.04-1.

Structurei

3D structure databases

ProteinModelPortaliP35669.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1534Substrate bindingBy similarity
Regioni233 – 2353Substrate bindingBy similarity
Regioni291 – 2944Substrate bindingBy similarity
Regioni484 – 4852Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.Curated

Phylogenomic databases

eggNOGiNOG329040.
HOGENOMiHOG000172641.
InParanoidiP35669.
KOiK01920.
OMAiEGRNIAT.
OrthoDBiEOG7GBG71.
PhylomeDBiP35669.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.30.470.20. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P35669-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEIEKYTPEQ IEELGKGARD FAFAHGVVFT ELSVSKEGRN IATQIPITLF
60 70 80 90 100
PSVIPHGAFV EAVSVQKAYN KLYAKIANDY EFLRLHLQSI TKYDEFMNKL
110 120 130 140 150
WNLYQKHREA VAHLKENQFQ PLSLGVFRSD YMVHQDDSFI GCKQVEFNTI
160 170 180 190 200
SVSFGGVSKA VSNLHAYCSQ SGLYRKPLTT NYLTVNTSVS GICTGISNAV
210 220 230 240 250
DAYRDYVKNI TSKMNIASDN TKPIVLFVVK GGERNITDQR TLEYELLNRF
260 270 280 290 300
HVISKRIDIA ELNSLIHDKS SNKLYMKTSF TTYEVAVVYY RVGYALDDYP
310 320 330 340 350
SQEAWDMRLT IENTLAIKCP SISTHLAGSK KIQQVLAESN ALERFLEGDE
360 370 380 390 400
LQAVRSTFAD MYPLDDTPRG KEGIKLAFEK PEDFVLKPQR EGGGNNTYGK
410 420 430 440 450
DIPGLLSKMP QEEWDSYILM RYINAVPSQN YILKGERPEK FDVVDEIGIL
460 470 480 490
GTIVWNIKTD EVVQNGQSGF ICRTKPKKTN EGGVATGYAS LSSIELSE
Length:498
Mass (Da):56,153
Last modified:October 1, 1996 - v2
Checksum:iAD2921C5AD667ABF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti132 – 1321M → T in AAL41009. 1 PublicationCurated
Sequence conflicti263 – 2631N → T in AAA35307. (PubMed:1958212)Curated
Sequence conflicti304 – 3041A → R in AAA35307. (PubMed:1958212)Curated
Sequence conflicti330 – 3301K → E in AAA35307. (PubMed:1958212)Curated
Sequence conflicti458 – 4581K → N in AAA35307. (PubMed:1958212)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93302.1.
AF448236 Genomic DNA. Translation: AAL41009.1.
Y08414 Genomic DNA. Translation: CAA69691.1.
M85179 Genomic DNA. Translation: AAA35307.1.
PIRiJT0961.
T38705.
RefSeqiNP_593936.1. NM_001019364.2.

Genome annotation databases

EnsemblFungiiSPAC3F10.04.1; SPAC3F10.04.1:pep; SPAC3F10.04.
GeneIDi2542896.
KEGGispo:SPAC3F10.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93302.1 .
AF448236 Genomic DNA. Translation: AAL41009.1 .
Y08414 Genomic DNA. Translation: CAA69691.1 .
M85179 Genomic DNA. Translation: AAA35307.1 .
PIRi JT0961.
T38705.
RefSeqi NP_593936.1. NM_001019364.2.

3D structure databases

ProteinModelPortali P35669.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 279340. 8 interactions.
MINTi MINT-4688612.
STRINGi 4896.SPAC3F10.04-1.

Proteomic databases

MaxQBi P35669.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC3F10.04.1 ; SPAC3F10.04.1:pep ; SPAC3F10.04 .
GeneIDi 2542896.
KEGGi spo:SPAC3F10.04.

Organism-specific databases

PomBasei SPAC3F10.04.

Phylogenomic databases

eggNOGi NOG329040.
HOGENOMi HOG000172641.
InParanoidi P35669.
KOi K01920.
OMAi EGRNIAT.
OrthoDBi EOG7GBG71.
PhylomeDBi P35669.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00210 .
BioCyci MetaCyc:MONOMER-10022.
Reactomei REACT_255388. Glutathione synthesis and recycling.

Miscellaneous databases

NextBioi 20803935.
PROi P35669.

Family and domain databases

Gene3Di 1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.30.470.20. 1 hit.
3.40.50.1760. 1 hit.
InterProi IPR013816. ATP_grasp_subdomain_2.
IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
PANTHERi PTHR11130. PTHR11130. 1 hit.
Pfami PF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001558. GSHase. 1 hit.
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Biosynthesis of phytochelatins in the fission yeast. Phytochelatin synthesis: a second role for the glutathione synthetase gene of Schizosaccharomyces pombe."
    Al-Lahham A., Rohde V., Heim P., Leuchter R., Veeck J., Wunderlich C., Wolf K., Zimmermann M.
    Yeast 15:385-396(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SP011.
  2. Shin Y.H., Lim C.-J.
    Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "Cloning and sequencing of the gene encoding the large subunit of glutathione synthetase of Schizosaccharomyces pombe."
    Mutoh N., Nakagawa C.W., Ando S., Tanabe K., Hayashi Y.
    Biochem. Biophys. Res. Commun. 181:430-436(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 214-498, PROTEIN SEQUENCE OF 218-229 AND 392-403.
    Strain: 972 / ATCC 24843.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-356, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiGSHB_SCHPO
AccessioniPrimary (citable) accession number: P35669
Secondary accession number(s): Q8X1A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3