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Protein

Glutathione synthetase large chain

Gene

gsa1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Putative glutamate--cysteine ligase regulatory subunit (SPCC737.06c), Glutamate--cysteine ligase (gcs1)
  2. Glutathione synthetase large chain (gsa1)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei128SubstrateBy similarity1
Metal bindingi146MagnesiumBy similarity1
Binding sitei146ATPBy similarity1
Metal bindingi148MagnesiumBy similarity1
Binding sitei239SubstrateBy similarity1
Binding sitei330ATPBy similarity1
Metal bindingi391MagnesiumBy similarity1
Binding sitei398ATPBy similarity1
Binding sitei446ATPBy similarity1
Binding sitei473SubstrateBy similarity1
Binding sitei475ATPBy similarity1
Binding sitei481ATP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi387 – 396ATPBy similarity10
Nucleotide bindingi420 – 423ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • cellular amino acid metabolic process Source: GO_Central
  • cellular detoxification of cadmium ion Source: PomBase
  • cellular response to cadmium ion Source: PomBase
  • glutathione biosynthetic process Source: PomBase
  • glutathione transport Source: PomBase
  • phytochelatin biosynthetic process Source: PomBase
  • phytochelatin-metal complex formation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10022.
BRENDAi6.3.2.3. 5613.
ReactomeiR-SPO-174403. Glutathione synthesis and recycling.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase large chain (EC:6.3.2.3)
Short name:
GSH synthetase large chain
Short name:
GSH-S
Alternative name(s):
Glutathione synthase large chain
Phytochelatin synthetase
Gene namesi
Name:gsa1
Synonyms:gsh2
ORF Names:SPAC3F10.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC3F10.04.
PomBaseiSPAC3F10.04. gsa1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • glutathione synthase complex Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002112651 – 498Glutathione synthetase large chainAdd BLAST498

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei356Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35669.
PRIDEiP35669.

PTM databases

iPTMnetiP35669.

Interactioni

Subunit structurei

Heterodimer composed of a large and a small chain.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi279340. 8 interactors.
MINTiMINT-4688612.

Structurei

3D structure databases

ProteinModelPortaliP35669.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 153Substrate bindingBy similarity4
Regioni233 – 235Substrate bindingBy similarity3
Regioni291 – 294Substrate bindingBy similarity4
Regioni484 – 485Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000172641.
InParanoidiP35669.
KOiK01920.
OMAiYMPRQYS.
OrthoDBiEOG092C36B7.
PhylomeDBiP35669.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.30.470.20. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 2 hits.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P35669-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIEKYTPEQ IEELGKGARD FAFAHGVVFT ELSVSKEGRN IATQIPITLF
60 70 80 90 100
PSVIPHGAFV EAVSVQKAYN KLYAKIANDY EFLRLHLQSI TKYDEFMNKL
110 120 130 140 150
WNLYQKHREA VAHLKENQFQ PLSLGVFRSD YMVHQDDSFI GCKQVEFNTI
160 170 180 190 200
SVSFGGVSKA VSNLHAYCSQ SGLYRKPLTT NYLTVNTSVS GICTGISNAV
210 220 230 240 250
DAYRDYVKNI TSKMNIASDN TKPIVLFVVK GGERNITDQR TLEYELLNRF
260 270 280 290 300
HVISKRIDIA ELNSLIHDKS SNKLYMKTSF TTYEVAVVYY RVGYALDDYP
310 320 330 340 350
SQEAWDMRLT IENTLAIKCP SISTHLAGSK KIQQVLAESN ALERFLEGDE
360 370 380 390 400
LQAVRSTFAD MYPLDDTPRG KEGIKLAFEK PEDFVLKPQR EGGGNNTYGK
410 420 430 440 450
DIPGLLSKMP QEEWDSYILM RYINAVPSQN YILKGERPEK FDVVDEIGIL
460 470 480 490
GTIVWNIKTD EVVQNGQSGF ICRTKPKKTN EGGVATGYAS LSSIELSE
Length:498
Mass (Da):56,153
Last modified:October 1, 1996 - v2
Checksum:iAD2921C5AD667ABF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti132M → T in AAL41009 (Ref. 2) Curated1
Sequence conflicti263N → T in AAA35307 (PubMed:1958212).Curated1
Sequence conflicti304A → R in AAA35307 (PubMed:1958212).Curated1
Sequence conflicti330K → E in AAA35307 (PubMed:1958212).Curated1
Sequence conflicti458K → N in AAA35307 (PubMed:1958212).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93302.1.
AF448236 Genomic DNA. Translation: AAL41009.1.
Y08414 Genomic DNA. Translation: CAA69691.1.
M85179 Genomic DNA. Translation: AAA35307.1.
PIRiJT0961.
T38705.
RefSeqiNP_593936.1. NM_001019364.2.

Genome annotation databases

EnsemblFungiiSPAC3F10.04.1; SPAC3F10.04.1:pep; SPAC3F10.04.
GeneIDi2542896.
KEGGispo:SPAC3F10.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93302.1.
AF448236 Genomic DNA. Translation: AAL41009.1.
Y08414 Genomic DNA. Translation: CAA69691.1.
M85179 Genomic DNA. Translation: AAA35307.1.
PIRiJT0961.
T38705.
RefSeqiNP_593936.1. NM_001019364.2.

3D structure databases

ProteinModelPortaliP35669.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279340. 8 interactors.
MINTiMINT-4688612.

PTM databases

iPTMnetiP35669.

Proteomic databases

MaxQBiP35669.
PRIDEiP35669.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC3F10.04.1; SPAC3F10.04.1:pep; SPAC3F10.04.
GeneIDi2542896.
KEGGispo:SPAC3F10.04.

Organism-specific databases

EuPathDBiFungiDB:SPAC3F10.04.
PomBaseiSPAC3F10.04. gsa1.

Phylogenomic databases

HOGENOMiHOG000172641.
InParanoidiP35669.
KOiK01920.
OMAiYMPRQYS.
OrthoDBiEOG092C36B7.
PhylomeDBiP35669.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.
BioCyciMetaCyc:MONOMER-10022.
BRENDAi6.3.2.3. 5613.
ReactomeiR-SPO-174403. Glutathione synthesis and recycling.

Miscellaneous databases

PROiP35669.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.30.470.20. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 2 hits.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGSHB_SCHPO
AccessioniPrimary (citable) accession number: P35669
Secondary accession number(s): Q8X1A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.