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P35669

- GSHB_SCHPO

UniProt

P35669 - GSHB_SCHPO

Protein

Glutathione synthetase large chain

Gene

gsa1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei128 – 1281SubstrateBy similarity
    Metal bindingi146 – 1461MagnesiumBy similarity
    Binding sitei146 – 1461ATPBy similarity
    Metal bindingi148 – 1481MagnesiumBy similarity
    Binding sitei239 – 2391SubstrateBy similarity
    Binding sitei330 – 3301ATPBy similarity
    Metal bindingi391 – 3911MagnesiumBy similarity
    Binding sitei398 – 3981ATPBy similarity
    Binding sitei446 – 4461ATPBy similarity
    Binding sitei473 – 4731SubstrateBy similarity
    Binding sitei475 – 4751ATPBy similarity
    Binding sitei481 – 4811ATP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi387 – 39610ATPBy similarity
    Nucleotide bindingi420 – 4234ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. glutathione binding Source: UniProtKB
    3. glutathione synthase activity Source: PomBase
    4. magnesium ion binding Source: UniProtKB
    5. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. cellular response to cadmium ion Source: PomBase
    2. detoxification of cadmium ion Source: PomBase
    3. glutathione biosynthetic process Source: PomBase
    4. glutathione transport Source: PomBase
    5. phytochelatin biosynthetic process Source: PomBase
    6. phytochelatin-metal complex formation Source: PomBase

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-10022.
    UniPathwayiUPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione synthetase large chain (EC:6.3.2.3)
    Short name:
    GSH synthetase large chain
    Short name:
    GSH-S
    Alternative name(s):
    Glutathione synthase large chain
    Phytochelatin synthetase
    Gene namesi
    Name:gsa1
    Synonyms:gsh2
    ORF Names:SPAC3F10.04
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC3F10.04.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: PomBase
    2. glutathione synthase complex Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 498498Glutathione synthetase large chainPRO_0000211265Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei356 – 3561Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP35669.

    Interactioni

    Subunit structurei

    Heterodimer composed of a large and a small chain.

    Protein-protein interaction databases

    BioGridi279340. 8 interactions.
    MINTiMINT-4688612.
    STRINGi4896.SPAC3F10.04-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP35669.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni150 – 1534Substrate bindingBy similarity
    Regioni233 – 2353Substrate bindingBy similarity
    Regioni291 – 2944Substrate bindingBy similarity
    Regioni484 – 4852Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the eukaryotic GSH synthase family.Curated

    Phylogenomic databases

    eggNOGiNOG329040.
    HOGENOMiHOG000172641.
    KOiK01920.
    OMAiEGRNIAT.
    OrthoDBiEOG7GBG71.
    PhylomeDBiP35669.

    Family and domain databases

    Gene3Di1.10.1080.10. 2 hits.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.1760. 1 hit.
    InterProiIPR013816. ATP_grasp_subdomain_2.
    IPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR11130. PTHR11130. 1 hit.
    PfamiPF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001558. GSHase. 1 hit.
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P35669-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEIEKYTPEQ IEELGKGARD FAFAHGVVFT ELSVSKEGRN IATQIPITLF    50
    PSVIPHGAFV EAVSVQKAYN KLYAKIANDY EFLRLHLQSI TKYDEFMNKL 100
    WNLYQKHREA VAHLKENQFQ PLSLGVFRSD YMVHQDDSFI GCKQVEFNTI 150
    SVSFGGVSKA VSNLHAYCSQ SGLYRKPLTT NYLTVNTSVS GICTGISNAV 200
    DAYRDYVKNI TSKMNIASDN TKPIVLFVVK GGERNITDQR TLEYELLNRF 250
    HVISKRIDIA ELNSLIHDKS SNKLYMKTSF TTYEVAVVYY RVGYALDDYP 300
    SQEAWDMRLT IENTLAIKCP SISTHLAGSK KIQQVLAESN ALERFLEGDE 350
    LQAVRSTFAD MYPLDDTPRG KEGIKLAFEK PEDFVLKPQR EGGGNNTYGK 400
    DIPGLLSKMP QEEWDSYILM RYINAVPSQN YILKGERPEK FDVVDEIGIL 450
    GTIVWNIKTD EVVQNGQSGF ICRTKPKKTN EGGVATGYAS LSSIELSE 498
    Length:498
    Mass (Da):56,153
    Last modified:October 1, 1996 - v2
    Checksum:iAD2921C5AD667ABF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti132 – 1321M → T in AAL41009. 1 PublicationCurated
    Sequence conflicti263 – 2631N → T in AAA35307. (PubMed:1958212)Curated
    Sequence conflicti304 – 3041A → R in AAA35307. (PubMed:1958212)Curated
    Sequence conflicti330 – 3301K → E in AAA35307. (PubMed:1958212)Curated
    Sequence conflicti458 – 4581K → N in AAA35307. (PubMed:1958212)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAA93302.1.
    AF448236 Genomic DNA. Translation: AAL41009.1.
    Y08414 Genomic DNA. Translation: CAA69691.1.
    M85179 Genomic DNA. Translation: AAA35307.1.
    PIRiJT0961.
    T38705.
    RefSeqiNP_593936.1. NM_001019364.2.

    Genome annotation databases

    EnsemblFungiiSPAC3F10.04.1; SPAC3F10.04.1:pep; SPAC3F10.04.
    GeneIDi2542896.
    KEGGispo:SPAC3F10.04.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAA93302.1 .
    AF448236 Genomic DNA. Translation: AAL41009.1 .
    Y08414 Genomic DNA. Translation: CAA69691.1 .
    M85179 Genomic DNA. Translation: AAA35307.1 .
    PIRi JT0961.
    T38705.
    RefSeqi NP_593936.1. NM_001019364.2.

    3D structure databases

    ProteinModelPortali P35669.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279340. 8 interactions.
    MINTi MINT-4688612.
    STRINGi 4896.SPAC3F10.04-1.

    Proteomic databases

    MaxQBi P35669.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC3F10.04.1 ; SPAC3F10.04.1:pep ; SPAC3F10.04 .
    GeneIDi 2542896.
    KEGGi spo:SPAC3F10.04.

    Organism-specific databases

    PomBasei SPAC3F10.04.

    Phylogenomic databases

    eggNOGi NOG329040.
    HOGENOMi HOG000172641.
    KOi K01920.
    OMAi EGRNIAT.
    OrthoDBi EOG7GBG71.
    PhylomeDBi P35669.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00210 .
    BioCyci MetaCyc:MONOMER-10022.

    Miscellaneous databases

    NextBioi 20803935.
    PROi P35669.

    Family and domain databases

    Gene3Di 1.10.1080.10. 2 hits.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.1760. 1 hit.
    InterProi IPR013816. ATP_grasp_subdomain_2.
    IPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR11130. PTHR11130. 1 hit.
    Pfami PF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001558. GSHase. 1 hit.
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Biosynthesis of phytochelatins in the fission yeast. Phytochelatin synthesis: a second role for the glutathione synthetase gene of Schizosaccharomyces pombe."
      Al-Lahham A., Rohde V., Heim P., Leuchter R., Veeck J., Wunderlich C., Wolf K., Zimmermann M.
      Yeast 15:385-396(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SP011.
    2. Shin Y.H., Lim C.-J.
      Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    4. "Cloning and sequencing of the gene encoding the large subunit of glutathione synthetase of Schizosaccharomyces pombe."
      Mutoh N., Nakagawa C.W., Ando S., Tanabe K., Hayashi Y.
      Biochem. Biophys. Res. Commun. 181:430-436(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 214-498, PROTEIN SEQUENCE OF 218-229 AND 392-403.
      Strain: 972 / ATCC 24843.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-356, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiGSHB_SCHPO
    AccessioniPrimary (citable) accession number: P35669
    Secondary accession number(s): Q8X1A1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3