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Protein

Glutathione synthetase

Gene

gss

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathway:iglutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glutathione synthetase (gssls-A-prov), Glutathione synthetase (gss)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251SubstrateBy similarity
Metal bindingi144 – 1441MagnesiumBy similarity
Binding sitei144 – 1441ATPBy similarity
Metal bindingi146 – 1461MagnesiumBy similarity
Binding sitei220 – 2201SubstrateBy similarity
Binding sitei305 – 3051ATPBy similarity
Metal bindingi368 – 3681MagnesiumBy similarity
Binding sitei375 – 3751ATPBy similarity
Binding sitei425 – 4251ATPBy similarity
Binding sitei450 – 4501SubstrateBy similarity
Binding sitei452 – 4521ATPBy similarity
Binding sitei458 – 4581ATP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi364 – 37310ATPBy similarity
Nucleotide bindingi398 – 4014ATPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Alternative name(s):
Glutathione synthase
Gene namesi
Name:gss
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6254454. gss.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Glutathione synthetasePRO_0000211264Add
BLAST

Expressioni

Tissue specificityi

Expressed ubiquitously.

Interactioni

Subunit structurei

Homodimer.Curated

Structurei

3D structure databases

ProteinModelPortaliP35668.
SMRiP35668. Positions 5-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1514Substrate bindingBy similarity
Regioni214 – 2163Substrate bindingBy similarity
Regioni267 – 2704Substrate bindingBy similarity
Regioni461 – 4622Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.Curated

Phylogenomic databases

HOVERGENiHBG002458.
KOiK01920.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P35668-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLWDDIYN DTKLLEELAP IAIDAALLQG VLMRTKESPN SSDVVSFAPF
60 70 80 90 100
ALLPSPVPKA LFEQAKCVQE DFNTLVDRIS QDTSFLEQVL SSTIKVDDFI
110 120 130 140 150
RRLFAIHKQV QQEDCTQEVF LGINRSDYMF DCRDDGTPAL KQIEINTIAA
160 170 180 190 200
SFGGLASRTP AVHQHVLKFL RKSEESSSIL TNDAVEGIGW GIAHAWALYG
210 220 230 240 250
SVDATVMFLV ENEQRNILDQ RFIEAELCKR NVRVIRRRLA DVFERGTLDE
260 270 280 290 300
ERHLFIDGYE VAVAYFRTGY VPQDYTEQDW EARLMLERSR AVKCPDVPTQ
310 320 330 340 350
LVGTKKVQQE LSRPQILEKF LPDKPEAVAR IRETFTGLYS LDIGEEGDEA
360 370 380 390 400
VRVALANPDQ FVLKPQREGG GNNLYGEELK EKLQECKDSE ERTSYILMDK
410 420 430 440 450
INPKPLKNCL LRAGGRVQIS ECISELGMFG VYVRHRDQMI YYDQVGHLLR
460 470
TKAIEHSDGG VAAGVAVLDN PYLV
Length:474
Mass (Da):53,584
Last modified:June 1, 1994 - v1
Checksum:i52CAA42C44079702
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69662 mRNA. Translation: CAA49351.1.
PIRiS38333.
RefSeqiNP_001081013.1. NM_001087544.1.
UniGeneiXl.1199.

Genome annotation databases

GeneIDi394329.
KEGGixla:394329.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69662 mRNA. Translation: CAA49351.1.
PIRiS38333.
RefSeqiNP_001081013.1. NM_001087544.1.
UniGeneiXl.1199.

3D structure databases

ProteinModelPortaliP35668.
SMRiP35668. Positions 5-474.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi394329.
KEGGixla:394329.

Organism-specific databases

CTDi2937.
XenbaseiXB-GENE-6254454. gss.

Phylogenomic databases

HOVERGENiHBG002458.
KOiK01920.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of the large subunit of glutathione synthetase from Xenopus laevis embryos."
    Habenicht A., Hille S., Knoechel W.
    Biochim. Biophys. Acta 1174:295-298(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiGSHB_XENLA
AccessioniPrimary (citable) accession number: P35668
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 26, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.