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P35668

- GSHB_XENLA

UniProt

P35668 - GSHB_XENLA

Protein

Glutathione synthetase

Gene

gss

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei125 – 1251SubstrateBy similarity
    Metal bindingi144 – 1441MagnesiumBy similarity
    Binding sitei144 – 1441ATPBy similarity
    Metal bindingi146 – 1461MagnesiumBy similarity
    Binding sitei220 – 2201SubstrateBy similarity
    Binding sitei305 – 3051ATPBy similarity
    Metal bindingi368 – 3681MagnesiumBy similarity
    Binding sitei375 – 3751ATPBy similarity
    Binding sitei425 – 4251ATPBy similarity
    Binding sitei450 – 4501SubstrateBy similarity
    Binding sitei452 – 4521ATPBy similarity
    Binding sitei458 – 4581ATP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi364 – 37310ATPBy similarity
    Nucleotide bindingi398 – 4014ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. glutathione binding Source: UniProtKB
    3. glutathione synthase activity Source: UniProtKB-EC
    4. magnesium ion binding Source: UniProtKB
    5. protein homodimerization activity Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione synthetase (EC:6.3.2.3)
    Short name:
    GSH synthetase
    Short name:
    GSH-S
    Alternative name(s):
    Glutathione synthase
    Gene namesi
    Name:gss
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-6254454. gss.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 474474Glutathione synthetasePRO_0000211264Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed ubiquitously.

    Interactioni

    Subunit structurei

    Homodimer.Curated

    Structurei

    3D structure databases

    ProteinModelPortaliP35668.
    SMRiP35668. Positions 5-474.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni148 – 1514Substrate bindingBy similarity
    Regioni214 – 2163Substrate bindingBy similarity
    Regioni267 – 2704Substrate bindingBy similarity
    Regioni461 – 4622Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the eukaryotic GSH synthase family.Curated

    Phylogenomic databases

    HOVERGENiHBG002458.
    KOiK01920.

    Family and domain databases

    Gene3Di1.10.1080.10. 2 hits.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 2 hits.
    3.40.50.1760. 1 hit.
    InterProiIPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR11130. PTHR11130. 1 hit.
    PfamiPF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001558. GSHase. 1 hit.
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P35668-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADLWDDIYN DTKLLEELAP IAIDAALLQG VLMRTKESPN SSDVVSFAPF    50
    ALLPSPVPKA LFEQAKCVQE DFNTLVDRIS QDTSFLEQVL SSTIKVDDFI 100
    RRLFAIHKQV QQEDCTQEVF LGINRSDYMF DCRDDGTPAL KQIEINTIAA 150
    SFGGLASRTP AVHQHVLKFL RKSEESSSIL TNDAVEGIGW GIAHAWALYG 200
    SVDATVMFLV ENEQRNILDQ RFIEAELCKR NVRVIRRRLA DVFERGTLDE 250
    ERHLFIDGYE VAVAYFRTGY VPQDYTEQDW EARLMLERSR AVKCPDVPTQ 300
    LVGTKKVQQE LSRPQILEKF LPDKPEAVAR IRETFTGLYS LDIGEEGDEA 350
    VRVALANPDQ FVLKPQREGG GNNLYGEELK EKLQECKDSE ERTSYILMDK 400
    INPKPLKNCL LRAGGRVQIS ECISELGMFG VYVRHRDQMI YYDQVGHLLR 450
    TKAIEHSDGG VAAGVAVLDN PYLV 474
    Length:474
    Mass (Da):53,584
    Last modified:June 1, 1994 - v1
    Checksum:i52CAA42C44079702
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69662 mRNA. Translation: CAA49351.1.
    PIRiS38333.
    RefSeqiNP_001081013.1. NM_001087544.1.
    UniGeneiXl.1199.

    Genome annotation databases

    GeneIDi394329.
    KEGGixla:394329.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69662 mRNA. Translation: CAA49351.1 .
    PIRi S38333.
    RefSeqi NP_001081013.1. NM_001087544.1.
    UniGenei Xl.1199.

    3D structure databases

    ProteinModelPortali P35668.
    SMRi P35668. Positions 5-474.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 394329.
    KEGGi xla:394329.

    Organism-specific databases

    CTDi 2937.
    Xenbasei XB-GENE-6254454. gss.

    Phylogenomic databases

    HOVERGENi HBG002458.
    KOi K01920.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00210 .

    Family and domain databases

    Gene3Di 1.10.1080.10. 2 hits.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 2 hits.
    3.40.50.1760. 1 hit.
    InterProi IPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR11130. PTHR11130. 1 hit.
    Pfami PF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001558. GSHase. 1 hit.
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the large subunit of glutathione synthetase from Xenopus laevis embryos."
      Habenicht A., Hille S., Knoechel W.
      Biochim. Biophys. Acta 1174:295-298(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiGSHB_XENLA
    AccessioniPrimary (citable) accession number: P35668
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3