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P35668

- GSHB_XENLA

UniProt

P35668 - GSHB_XENLA

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Protein

Glutathione synthetase

Gene
gss
Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251Substrate By similarity
Metal bindingi144 – 1441Magnesium By similarity
Binding sitei144 – 1441ATP By similarity
Metal bindingi146 – 1461Magnesium By similarity
Binding sitei220 – 2201Substrate By similarity
Binding sitei305 – 3051ATP By similarity
Metal bindingi368 – 3681Magnesium By similarity
Binding sitei375 – 3751ATP By similarity
Binding sitei425 – 4251ATP By similarity
Binding sitei450 – 4501Substrate By similarity
Binding sitei452 – 4521ATP By similarity
Binding sitei458 – 4581ATP; via carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi364 – 37310ATP By similarity
Nucleotide bindingi398 – 4014ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. glutathione binding Source: UniProtKB
  3. glutathione synthase activity Source: UniProtKB-EC
  4. magnesium ion binding Source: UniProtKB
  5. protein homodimerization activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Alternative name(s):
Glutathione synthase
Gene namesi
Name:gss
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6254454. gss.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Glutathione synthetasePRO_0000211264Add
BLAST

Expressioni

Tissue specificityi

Expressed ubiquitously.

Interactioni

Subunit structurei

Homodimer Reviewed prediction.

Structurei

3D structure databases

ProteinModelPortaliP35668.
SMRiP35668. Positions 5-474.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1514Substrate binding By similarity
Regioni214 – 2163Substrate binding By similarity
Regioni267 – 2704Substrate binding By similarity
Regioni461 – 4622Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG002458.
KOiK01920.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P35668-1 [UniParc]FASTAAdd to Basket

« Hide

MADLWDDIYN DTKLLEELAP IAIDAALLQG VLMRTKESPN SSDVVSFAPF    50
ALLPSPVPKA LFEQAKCVQE DFNTLVDRIS QDTSFLEQVL SSTIKVDDFI 100
RRLFAIHKQV QQEDCTQEVF LGINRSDYMF DCRDDGTPAL KQIEINTIAA 150
SFGGLASRTP AVHQHVLKFL RKSEESSSIL TNDAVEGIGW GIAHAWALYG 200
SVDATVMFLV ENEQRNILDQ RFIEAELCKR NVRVIRRRLA DVFERGTLDE 250
ERHLFIDGYE VAVAYFRTGY VPQDYTEQDW EARLMLERSR AVKCPDVPTQ 300
LVGTKKVQQE LSRPQILEKF LPDKPEAVAR IRETFTGLYS LDIGEEGDEA 350
VRVALANPDQ FVLKPQREGG GNNLYGEELK EKLQECKDSE ERTSYILMDK 400
INPKPLKNCL LRAGGRVQIS ECISELGMFG VYVRHRDQMI YYDQVGHLLR 450
TKAIEHSDGG VAAGVAVLDN PYLV 474
Length:474
Mass (Da):53,584
Last modified:June 1, 1994 - v1
Checksum:i52CAA42C44079702
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69662 mRNA. Translation: CAA49351.1.
PIRiS38333.
RefSeqiNP_001081013.1. NM_001087544.1.
UniGeneiXl.1199.

Genome annotation databases

GeneIDi394329.
KEGGixla:394329.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69662 mRNA. Translation: CAA49351.1 .
PIRi S38333.
RefSeqi NP_001081013.1. NM_001087544.1.
UniGenei Xl.1199.

3D structure databases

ProteinModelPortali P35668.
SMRi P35668. Positions 5-474.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 394329.
KEGGi xla:394329.

Organism-specific databases

CTDi 2937.
Xenbasei XB-GENE-6254454. gss.

Phylogenomic databases

HOVERGENi HBG002458.
KOi K01920.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00210 .

Family and domain databases

Gene3Di 1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProi IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
PANTHERi PTHR11130. PTHR11130. 1 hit.
Pfami PF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001558. GSHase. 1 hit.
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of the large subunit of glutathione synthetase from Xenopus laevis embryos."
    Habenicht A., Hille S., Knoechel W.
    Biochim. Biophys. Acta 1174:295-298(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiGSHB_XENLA
AccessioniPrimary (citable) accession number: P35668
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 16, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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