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P35668 (GSHB_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

Short name=GSH synthetase
Short name=GSH-S
EC=6.3.2.3
Alternative name(s):
Glutathione synthase
Gene names
Name:gss
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.

Subunit structure

Homodimer Potential.

Tissue specificity

Expressed ubiquitously.

Sequence similarities

Belongs to the eukaryotic GSH synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 474474Glutathione synthetase
PRO_0000211264

Regions

Nucleotide binding364 – 37310ATP By similarity
Nucleotide binding398 – 4014ATP By similarity
Region148 – 1514Substrate binding By similarity
Region214 – 2163Substrate binding By similarity
Region267 – 2704Substrate binding By similarity
Region461 – 4622Substrate binding By similarity

Sites

Metal binding1441Magnesium By similarity
Metal binding1461Magnesium By similarity
Metal binding3681Magnesium By similarity
Binding site1251Substrate By similarity
Binding site1441ATP By similarity
Binding site2201Substrate By similarity
Binding site3051ATP By similarity
Binding site3751ATP By similarity
Binding site4251ATP By similarity
Binding site4501Substrate By similarity
Binding site4521ATP By similarity
Binding site4581ATP; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
P35668 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 52CAA42C44079702

FASTA47453,584
        10         20         30         40         50         60 
MADLWDDIYN DTKLLEELAP IAIDAALLQG VLMRTKESPN SSDVVSFAPF ALLPSPVPKA 

        70         80         90        100        110        120 
LFEQAKCVQE DFNTLVDRIS QDTSFLEQVL SSTIKVDDFI RRLFAIHKQV QQEDCTQEVF 

       130        140        150        160        170        180 
LGINRSDYMF DCRDDGTPAL KQIEINTIAA SFGGLASRTP AVHQHVLKFL RKSEESSSIL 

       190        200        210        220        230        240 
TNDAVEGIGW GIAHAWALYG SVDATVMFLV ENEQRNILDQ RFIEAELCKR NVRVIRRRLA 

       250        260        270        280        290        300 
DVFERGTLDE ERHLFIDGYE VAVAYFRTGY VPQDYTEQDW EARLMLERSR AVKCPDVPTQ 

       310        320        330        340        350        360 
LVGTKKVQQE LSRPQILEKF LPDKPEAVAR IRETFTGLYS LDIGEEGDEA VRVALANPDQ 

       370        380        390        400        410        420 
FVLKPQREGG GNNLYGEELK EKLQECKDSE ERTSYILMDK INPKPLKNCL LRAGGRVQIS 

       430        440        450        460        470 
ECISELGMFG VYVRHRDQMI YYDQVGHLLR TKAIEHSDGG VAAGVAVLDN PYLV 

« Hide

References

[1]"Molecular cloning of the large subunit of glutathione synthetase from Xenopus laevis embryos."
Habenicht A., Hille S., Knoechel W.
Biochim. Biophys. Acta 1174:295-298(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69662 mRNA. Translation: CAA49351.1.
PIRS38333.
RefSeqNP_001081013.1. NM_001087544.1.
UniGeneXl.1199.

3D structure databases

ProteinModelPortalP35668.
SMRP35668. Positions 5-474.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID394329.
KEGGxla:394329.

Organism-specific databases

CTD2937.
XenbaseXB-GENE-6254454. gss.

Phylogenomic databases

HOVERGENHBG002458.
KOK01920.

Enzyme and pathway databases

UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR11130. PTHR11130. 1 hit.
PfamPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001558. GSHase. 1 hit.
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01986. glut_syn_euk. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGSHB_XENLA
AccessionPrimary (citable) accession number: P35668
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 16, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways