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P35667 (GSHB_ANACE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Synonyms:gsh-II
OrganismAnaplasma centrale
Taxonomic identifier769 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeAnaplasma

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 308308Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197451

Regions

Domain117 – 300184ATP-grasp
Nucleotide binding143 – 19856ATP By similarity

Sites

Metal binding2711Magnesium or manganese By similarity
Metal binding2731Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
P35667 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: AD1E019332AAB38F

FASTA30834,512
        10         20         30         40         50         60 
MLKVAFQMDE DVVVGRDVSV KLVEEAQRRG HEVFFYRPTS LAFVCGELVA EAFSVRVGAD 

        70         80         90        100        110        120 
SLHFHDKTRL PLGKLDMLFV RQNPPFDMRY VTTTYLLERL DILMINNPKA IRDHPEKLLP 

       130        140        150        160        170        180 
LSFPKFIPPT LITESVSEIS AFYAEYGDIV LKPLYDYGGN GVCRICGRAD VGAISSAMVE 

       190        200        210        220        230        240 
RYEAPLVAQQ FIDDISSDKR VVLLGGRPIG AVRRKVTAMG EIRTNLRVGA TPEATELSDR 

       250        260        270        280        290        300 
EREICHDVGM LLSSVDILFA GIDILGGHLI EVNVTSPCGI LEINQVYGKT LERDCWDYFE 


YALLHRSP 

« Hide

References

[1]"Sequence of a putative glutathione synthetase II gene and flanking regions from Anaplasma centrale."
Peters J.M., Dalrymple B.P., Jorgensen W.K.
Biochem. Biophys. Res. Commun. 182:1040-1046(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M80425 Genomic DNA. Translation: AAA22064.1.
PIRJQ1401.

3D structure databases

ProteinModelPortalP35667.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG0189.

Enzyme and pathway databases

UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_ANACE
AccessionPrimary (citable) accession number: P35667
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 19, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways