P35661 (GST27_SCHMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase class-mu 26 kDa isozyme
Short name=GST 26
EC=2.5.1.18

Alternative name(s):
Sm26/2 antigen

Gene names

ORF Names:

Smp_102070

Organism

Schistosoma mansoni (Blood fluke) [Reference proteome]

Taxonomic identifier

6183 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Platyhelminthes › Trematoda › Digenea › Strigeidida › Schistosomatoidea › Schistosomatidae › Schistosoma

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the GST superfamily. Mu family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	Glutathione S-transferase class-mu 26 kDa isozyme		PRO_0000185811
Regions
	Domain	1 – 83	83	GST N-terminal
	Domain	85 – 195	111	GST C-terminal
	Region	7 – 8	2	Glutathione binding By similarity
	Region	41 – 45	5	Glutathione binding By similarity
	Region	54 – 55	2	Glutathione binding By similarity
	Region	67 – 68	2	Glutathione binding By similarity
Sites
	Binding site	111	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35661 [UniParc]. Last modified June 13, 2012. Version 2. Checksum: 7FF9CA7AB3E445B9	FASTA	195	22,546
	10 20 30 40 50 60 MAPKLGYWKI KGLVQPTRLL LEYLGEAYEE RLYDRNDGDV WRNEKFKLGL DFPNLPYYID 70 80 90 100 110 120 GDVKLTQSMA ILRYIADKHN MLGGCPKERA EISMLEGAIL DIRYGVSRIA YNKEFETLKV 130 140 150 160 170 180 DFLNQLPGML KMFEDRLSHN TYLNGDKVTH PDFMLYDALD VNLPPIKNYL NSNRYIKWPL 190 QGWSATFGGG DAPPK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Another 26-kilodalton glutathione S-transferase of Schistosoma mansoni." Wright M.D., Harrison R.A., Melder A.M., Newport G.R., Mitchell G.F. Mol. Biochem. Parasitol. 49:177-179(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome of the blood fluke Schistosoma mansoni." Berriman M., Haas B.J., LoVerde P.T., Wilson R.A., Dillon G.P., Cerqueira G.C., Mashiyama S.T., Al-Lazikani B., Andrade L.F., Ashton P.D., Aslett M.A., Bartholomeu D.C., Blandin G., Caffrey C.R., Coghlan A., Coulson R., Day T.A., Delcher A. , DeMarco R., Djikeng A., Eyre T., Gamble J.A., Ghedin E., Gu Y., Hertz-Fowler C., Hirai H., Hirai Y., Houston R., Ivens A., Johnston D.A., Lacerda D., Macedo C.D., McVeigh P., Ning Z., Oliveira G., Overington J.P., Parkhill J., Pertea M., Pierce R.J., Protasio A.V., Quail M.A., Rajandream M.A., Rogers J., Sajid M., Salzberg S.L., Stanke M., Tivey A.R., White O., Williams D.L., Wortman J., Wu W., Zamanian M., Zerlotini A., Fraser-Liggett C.M., Barrell B.G., El-Sayed N.M. Nature 460:352-358(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Puerto Rican.
[3]	"A systematically improved high quality genome and transcriptome of the human blood fluke Schistosoma mansoni." Protasio A.V., Tsai I.J., Babbage A., Nichol S., Hunt M., Aslett M.A., De Silva N., Velarde G.S., Anderson T.J., Clark R.C., Davidson C., Dillon G.P., Holroyd N.E., LoVerde P.T., Lloyd C., McQuillan J., Oliveira G., Otto T.D. , Parker-Manuel S.J., Quail M.A., Wilson R.A., Zerlotini A., Dunne D.W., Berriman M. PLoS Negl. Trop. Dis. 6:E1455-E1455(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Puerto Rican.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M73624 mRNA. No translation available. HE601628 Genomic DNA. Translation: CCD80234.1.
PIR	A45556.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOGENOM	HOG000115735.
PhylomeDB	P35661.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GST27_SCHMA

Accession

Primary (citable) accession number: P35661
Secondary accession number(s): G4VKM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 13, 2012
Last modified:	April 3, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)