P35660 (DDL_ANACE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-alanine--D-alanine ligase
EC=6.3.2.4

Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase

Gene names

Name:

ddl

Organism

Anaplasma centrale

Taxonomic identifier

769 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma

Protein attributes

Sequence length	205 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. HAMAP-Rule MF_00047
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›205	›205	D-alanine--D-alanine ligase HAMAP-Rule MF_00047		PRO_0000177778
Regions
	Domain	111 – ›205	›95	ATP-grasp
	Nucleotide binding	139 – 190	52	ATP By similarity
Experimental info
	Non-terminal residue	205	1

Sequences

	Sequence		Length	Mass (Da)
	P35660 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 0FC7855DFB10A636	FASTA	205	21,656
	10 20 30 40 50 60 MPVGLACNAD DVLSIAVLCG GSSPEREVSL AGGKRIADAL GRLGHRAAVV DLNRESAHQL 70 80 90 100 110 120 LAMAPDLVYN ALHGGQGEDG CASGLLDILG LAYTHSRVAA SSVGMDKVLT KHVLKSLGID 130 140 150 160 170 180 FPEFSVLTKE EVLSAKEVMP YPFVIKPICG GSTIGVHAIF SRSEYLDLSV HADALEGRML 190 200 VEEYIPGQEV HTAVFLGRAI GTMEF

References

[1]	"Sequence of a putative glutathione synthetase II gene and flanking regions from Anaplasma centrale." Peters J.M., Dalrymple B.P., Jorgensen W.K. Biochem. Biophys. Res. Commun. 182:1040-1046(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80425 Genomic DNA. Translation: AAA22065.1.
PIR	PQ0272.
3D structure databases
ProteinModelPortal	P35660.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG1181.
Enzyme and pathway databases
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 2 hits. 3.40.50.20. 1 hit.
HAMAP	MF_00047. Dala_Dala_lig.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom. [Graphical view]
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF01820. Dala_Dala_lig_N. 2 hits. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_ANACE

Accession

Primary (citable) accession number: P35660

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program