Reviewed, UniProtKB/Swiss-Prot P35638 (DDIT3_HUMAN)

Names and origin

Protein names

Recommended name:
    DNA damage-inducible transcript 3
      Short name=DDIT-3
Alternative name(s):
    Growth arrest and DNA-damage-inducible protein GADD153
    C/EBP-homologous protein
      Short name=CHOP

Gene names

Name:	DDIT3
Synonyms:	CHOP, GADD153

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA.
Subunit structure	Heterodimer.
Subcellular location	Nucleus.
Involvement in disease	A chromosomal aberration involving DDIT3 is found in a form of malignant myxoid liposarcoma [MIM:126337]. Translocation t(12;16)(q13;p11) with FUS.
Sequence similarities	Belongs to the bZIP family. Contains 1 bZIP domain.

Ontologies

Keywords
Biological process	Cell cycle Growth arrest Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Chromosomal rearrangement Polymorphism
Disease	Proto-oncogene
Molecular function	Repressor
Gene Ontology (GO)
Biological process	cell cycle arrest Inferred from electronic annotation. Source: UniProtKB-KW cell redox homeostasis Inferred from direct assay. Source: MGI mRNA transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI regulation of transcription, DNA-dependent Ref.3 Traceable author statement. Source: ProtInc response to DNA damage stimulus Ref.1 Traceable author statement. Source: ProtInc
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein dimerization activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription corepressor activity Ref.3 Traceable author statement. Source: ProtInc transcription factor activity Ref.2 Non-traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	DNA damage-inducible transcript 3		PRO_0000076642
Regions
	Domain	134 – 148	15	Leucine-zipper
	Compositional bias	93 – 97	5	Poly-Glu
Natural variations
	Natural variant	115	1	A → V in a colorectal cancer sample; somatic mutation. Ref.6		VAR_036000
Experimental info
	Sequence conflict	10 – 14	5	FGTLS → SDTV in AAB22646. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P35638-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 31905293FB1FBBE2	FASTA	169	19,175
	10 20 30 40 50 60 MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK IFTTLDPASL 70 80 90 100 110 120 AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG RTRKRKQSGH SPARAGKQRM 130 140 150 160 KEKEQENERK VAQLAEENER LKQEIERLTR EVEATRRALI DRMVNLHQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, characterization and chromosomal localization of the human GADD153 gene." Park J.S., Luethy J.D., Wang M.G., Fargnoli J., Fornace A.J. Jr., McBride O.W., Holbrook N.J. Gene 116:259-267(1992) [PubMed: 1339368] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Fusion of CHOP to a novel RNA-binding protein in human myxoid liposarcoma." Crozat A., Aman P., Mandahl N., Ron D. Nature 363:640-644(1993) [PubMed: 8510758] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Fusion of the dominant negative transcription regulator CHOP with a novel gene FUS by translocation t(12;16) in malignant liposarcoma." Rabbitts T.H., Forster A., Larson R., Nathan P. Nat. Genet. 4:175-180(1993) [PubMed: 7503811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHROMOSOMAL TRANSLOCATION WITH FUS.
[4]	NIEHS SNPs program Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] VAL-115.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

NIEHS-SNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	S40706 mRNA. Translation: AAB22646.1. S62138 mRNA. Translation: AAB27103.1. Different initiation. AY880949 Genomic DNA. Translation: AAW56077.1. BC003637 mRNA. Translation: AAH03637.1.
IPI	IPI00019956.
PIR	JC1169. S33798.
RefSeq	NP_004074.2.
UniGene	Hs.505777 Hs.690217
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P35638. 5 interactions.
PTM databases
PhosphoSite	P35638.
Genome annotation databases
Ensembl	ENSG00000175197. Homo sapiens. [Contig view]
GeneID	1649.
KEGG	hsa:1649.
Organism-specific databases
GeneCards	GC12M056196.
H-InvDB	HIX0010762.
HGNC	HGNC:2726. DDIT3.
HPA	CAB008969.
MIM	126337. gene+phenotype.
PharmGKB	PA27193.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P35638.
HOVERGEN	P35638.
OMA	P35638. WELEAWY.
Enzyme and pathway databases
Pathway_Interaction_DB	p38alphabetadownstreampathway. Signaling mediated by p38-alpha and p38-beta.
Gene expression databases
ArrayExpress	P35638.
Bgee	P35638.
CleanEx	HS_DDIT3.
GermOnline	ENSG00000175197. Homo sapiens.
Family and domain databases
InterPro	IPR011700. bZIP_2. IPR016670. DNA_damage_induc_transcript_3. IPR004827. TF_bZIP. [Graphical view]
Pfam	PF07716. bZIP_2. 1 hit. [Graphical view]
PIRSF	PIRSF016571. C/EBPzeta_CHOP_DDIT3. 1 hit.
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	6792.
SOURCE	Search...

Entry information

Entry name

DDIT3_HUMAN

Accession

Primary (citable) accession number: P35638

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)