P35638 (DDIT3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA damage-inducible transcript 3 protein
Short name=DDIT-3

Alternative name(s):
C/EBP-homologous protein
Short name=CHOP
C/EBP-homologous protein 10
Short name=CHOP-10
Growth arrest and DNA damage-inducible protein GADD153

Gene names

Name:	DDIT3
Synonyms:	CHOP, CHOP10, GADD153

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA.
Subunit structure	Heterodimer.
Subcellular location	Nucleus.
Involvement in disease	Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS.
Sequence similarities	Belongs to the bZIP family. Contains 1 bZIP domain.
Sequence caution	The sequence AAB27103.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Growth arrest Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Chromosomal rearrangement Polymorphism
Disease	Proto-oncogene
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle arrest Inferred from electronic annotation. Source: UniProtKB-KW cell redox homeostasis Inferred from direct assay. Source: MGI mRNA transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI negative regulation of determination of dorsal identity Inferred from direct assay. Source: BHF-UCL negative regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay. Source: BHF-UCL regulation of DNA-dependent transcription in response to stress Traceable author statement. Source: BHF-UCL response to DNA damage stimulus Traceable author statement. Source: ProtInc response to endoplasmic reticulum stress Inferred from direct assay. Source: BHF-UCL
Cellular component	endoplasmic reticulum membrane Traceable author statement. Source: Reactome nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein dimerization activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Non-traceable author statement. Source: ProtInc transcription corepressor activity Traceable author statement. Source: ProtInc transcription factor binding Inferred from physical interaction. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	DNA damage-inducible transcript 3 protein		PRO_0000076642
Regions
	Domain	134 – 148	15	Leucine-zipper
	Compositional bias	93 – 97	5	Poly-Glu
Natural variations
	Natural variant	115	1	A → V in a colorectal cancer sample; somatic mutation. Ref.6		VAR_036000
Experimental info
	Sequence conflict	10 – 14	5	FGTLS → SDTV in AAB22646. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P35638 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 31905293FB1FBBE2	FASTA	169	19,175
	10 20 30 40 50 60 MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK IFTTLDPASL 70 80 90 100 110 120 AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG RTRKRKQSGH SPARAGKQRM 130 140 150 160 KEKEQENERK VAQLAEENER LKQEIERLTR EVEATRRALI DRMVNLHQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, characterization and chromosomal localization of the human GADD153 gene." Park J.S., Luethy J.D., Wang M.G., Fargnoli J., Fornace A.J. Jr., McBride O.W., Holbrook N.J. Gene 116:259-267(1992) [PubMed: 1339368] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Fusion of CHOP to a novel RNA-binding protein in human myxoid liposarcoma." Crozat A., Aman P., Mandahl N., Ron D. Nature 363:640-644(1993) [PubMed: 8510758] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Fusion of the dominant negative transcription regulator CHOP with a novel gene FUS by translocation t(12;16) in malignant liposarcoma." Rabbitts T.H., Forster A., Larson R., Nathan P. Nat. Genet. 4:175-180(1993) [PubMed: 7503811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHROMOSOMAL TRANSLOCATION WITH FUS.
[4]	NIEHS SNPs program Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] VAL-115.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	S40706 mRNA. Translation: AAB22646.1. S62138 mRNA. Translation: AAB27103.1. Different initiation. AY880949 Genomic DNA. Translation: AAW56077.1. BC003637 mRNA. Translation: AAH03637.1.
IPI	IPI00019956.
PIR	JC1169. S33798.
RefSeq	NP_001181986.1. NM_001195057.1. NP_004074.2. NM_004083.5.
UniGene	Hs.505777. Hs.728989.
3D structure databases
ProteinModelPortal	P35638.
SMR	P35638. Positions 103-160.
ModBase	Search...
Protein-protein interaction databases
IntAct	P35638. 16 interactions.
MINT	MINT-1434413.
STRING	P35638.
PTM databases
PhosphoSite	P35638.
Polymorphism databases
DMDM	544022.
Proteomic databases
PRIDE	P35638.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000346473; ENSP00000340671; ENSG00000175197.
GeneID	1649.
KEGG	hsa:1649.
UCSC	uc001soi.1. human.
Organism-specific databases
CTD	1649.
GeneCards	GC12M057910.
H-InvDB	HIX0010762.
HGNC	HGNC:2726. DDIT3.
MIM	126337. gene+phenotype.
neXtProt	NX_P35638.
PharmGKB	PA27193.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18702.
HOGENOM	HBG127272.
HOVERGEN	HBG051328.
InParanoid	P35638.
OMA	WELEAWY.
OrthoDB	EOG480HZ0.
PhylomeDB	P35638.
Enzyme and pathway databases
Pathway_Interaction_DB	p38alphabetadownstreampathway. Signaling mediated by p38-alpha and p38-beta.
Reactome	REACT_15380. Diabetes pathways.
Gene expression databases
ArrayExpress	P35638.
Bgee	P35638.
CleanEx	HS_DDIT3.
Genevestigator	P35638.
GermOnline	ENSG00000175197. Homo sapiens.
Family and domain databases
InterPro	IPR004827. bZIP. IPR011700. bZIP_2. IPR016670. DNA_damage_induc_transcript_3. [Graphical view]
KO	K04452.
PANTHER	PTHR16833. PTHR16833. 1 hit.
Pfam	PF07716. bZIP_2. 1 hit. [Graphical view]
PIRSF	PIRSF016571. C/EBPzeta_CHOP_DDIT3. 1 hit.
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	6792.
SOURCE	Search...

Entry information

Entry name

DDIT3_HUMAN

Accession

Primary (citable) accession number: P35638

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 25, 2012
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.