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Reviewed, UniProtKB/Swiss-Prot P35636 (SCR_ZYMMO)

Last modified November 3, 2009. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Sucrose-6-phosphate hydrolase
      Short name=Sucrase
    EC=3.2.1.26
Alternative name(s):
    Invertase
Gene names
Name: sacA
Synonyms: invA
Ordered Locus Names: ZMO0942
OrganismZymomonas mobilis [Complete proteome] [HAMAP]
Taxonomic identifier542 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas

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Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.

Pathway

Glycan biosynthesis; sucrose metabolism.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glycosyl hydrolase 32 family.

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Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentCytoplasm
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionbeta-fructofuranosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Sucrose-6-phosphate hydrolase
PRO_0000169880

Regions

Region40 – 434Substrate binding By similarity
Region102 – 1032Substrate binding By similarity
Region165 – 1662Substrate binding By similarity

Sites

Active site431 By similarity
Binding site591Substrate By similarity
Binding site671Substrate By similarity
Binding site2291Substrate By similarity
Binding site3111Substrate By similarity

Experimental info

Sequence conflict241L → V in BAA01258. Ref.2
Sequence conflict391G → S in AAA27701. Ref.1
Sequence conflict951S → L in AAO38865. Ref.3
Sequence conflict1121V → I in BAA01258. Ref.2
Sequence conflict1281L → P in AAA27701. Ref.1
Sequence conflict1281L → P in BAA01258. Ref.2
Sequence conflict1281L → P in AAO38865. Ref.3
Sequence conflict1521V → I in BAA01258. Ref.2
Sequence conflict1561A → P in BAA01258. Ref.2
Sequence conflict1741N → D in AAA27701. Ref.1
Sequence conflict1741N → D in BAA01258. Ref.2
Sequence conflict1741N → D in AAO38865. Ref.3
Sequence conflict1751H → R AA sequence Ref.2
Sequence conflict1871E → K in AAA27701. Ref.1
Sequence conflict295 – 2973FEA → LKL in AAA27701. Ref.1
Sequence conflict2971A → D in BAA01258. Ref.2
Sequence conflict303 – 3086ILIAWF → NINCMV in AAA27701. Ref.1
Sequence conflict312 – 3143ENQ → KS in AAA27701. Ref.1
Sequence conflict3711M → I in BAA01258. Ref.2
Sequence conflict3881S → N in AAA27701. Ref.1
Sequence conflict4001G → D in BAA01258. Ref.2
Sequence conflict5081S → L in BAA01258. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
P35636-1 [UniParc].

Last modified February 15, 2005. Version 2.
Checksum: 3EFBF2499FFD8CAD

FASTA51258,575
        10         20         30         40         50         60 
MESPSYKNLI KAEDAQKKAG KRLLSSEWYP GFHVTPLTGW MNDPNGLIFF KGEYHLFYQY 

        70         80         90        100        110        120 
YPFAPVWGPM HWGHAKSRDL VHWETLPVAL APGDSFDRDG CFSGCAVDNN GVLTLIYTGH 

       130        140        150        160        170        180 
IVLSNDSLDA IREVQCMATS IDGIHFQKEG IVLEKAPMPQ VAHFRDPRVW KENNHWFMVV 

       190        200        210        220        230        240 
GYRTDDEKHQ GIGHVALYRS ENLKDWIFVK TLLGDNSQLP LGKRAFMWEC PDFFSLGNRS 

       250        260        270        280        290        300 
VLMFSPQGLK ASGYKNRNLF QNGYILGKWQ APQFTPETSF QELDYGHDFY AAQRFEAKDG 

       310        320        330        340        350        360 
RQILIAWFDM WENQKPSQRD GWAGCMTLPR KLDLIDNKIV MTPVREMEIL RQSEKIESVV 

       370        380        390        400        410        420 
TLSDAEHPFT MDSPLQEIEL IFDLEKSSAY QAGLALRCNG KGQETLLYID RSQNRIILDR 

       430        440        450        460        470        480 
NRSGQNVKGI RSCPLPNTSK VRLHIFLDRS SIEIFVGDDQ TQGLYSISSR IFPDKDSLKG 

       490        500        510 
RLFAIEGYAV FDSFKRWTLQ DANLAAFSSD AC

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References

« Hide 'large scale' references
[1]"Cloning and sequencing of the sacA gene: characterization of a sucrase from Zymomonas mobilis."
Gunasekaran P., Karunakaran T., Cami B., Mukundan A.G., Preziosi L., Baratti J.
J. Bacteriol. 172:6727-6735(1990) [PubMed: 2254250] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 10988 / NCIB 8938 / NRRL B-806 / ZM1.
[2]"Cloning, sequencing, and characterization of the intracellular invertase gene from Zymomonas mobilis."
Yanase H., Fukushi H., Ueda N., Maeda Y., Toyoda A., Tonomura K.
Agric. Biol. Chem. 55:1383-1390(1991) [PubMed: 1368686] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: Z6C.
[3]"Sequence of a probable nucleotide sugar epimerase and guanine deaminase from Zymomonas mobilis."
O'Mullan P.J., Chase T. Jr., Eveleigh D.E.
Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4."
Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. expand/collapse author list , Kang H.L., Lee S.Y., Lee K.J., Kang H.S.
Nat. Biotechnol. 23:63-68(2005) [PubMed: 15592456] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.

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Cross-references

Sequence databases

M62718 Genomic DNA. Translation: AAA27701.1.
D10465 Genomic DNA. Translation: BAA01258.1.
AY171597 Genomic DNA. Translation: AAO38865.1.
AE008692 Genomic DNA. Translation: AAV89566.1.
PIRA37803.
JU0460.
RefSeqYP_162677.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGH32. Glycoside Hydrolase Family 32.

Genome annotation databases

GeneID3187940.
GenomeReviewsGene locus ZMO0942 in contig AE008692_GR.
KEGGzmo:ZMO0942.
NMPDRfig|264203.3.peg.1780.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP35636.
OMARRIVIGW.

Enzyme and pathway databases

BioCycZMOB264203:ZMO0942-MON.
BRENDA3.2.1.26. 1658.

Family and domain databases

InterProIPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR006232. Suc6P_hydrolase.
[Graphical view]
PfamPF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTSM00640. Glyco_32. 1 hit.
[Graphical view]
TIGRFAMsTIGR01322. scrB_fam. 1 hit.
PROSITEPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameSCR_ZYMMO
AccessionPrimary (citable) accession number: P35636
Secondary accession number(s): P22632, Q5NNZ4, Q84CM3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 15, 2005
Last modified: November 3, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents