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Protein

Floral homeotic protein APETALA 3

Gene

AP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable transcription factor involved in the genetic control of flower development. Is required for normal development of petals and stamens in the wild-type flower. Forms a heterodimer with PISTILLATA that is required for autoregulation of both AP3 and PI genes. AP3/PI heterodimer interacts with APETALA1 or SEPALLATA3 to form a ternary complex that could be responsible for the regulation of the genes involved in the flower development. AP3/PI heterodimer activates the expression of NAP.2 Publications

Miscellaneous

Mutations in AP3 cause transformation of petals into sepals and stamina into carpels.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Flowering, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Floral homeotic protein APETALA 3
Gene namesi
Name:AP3
Ordered Locus Names:At3g54340
ORF Names:T12E18_30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G54340
TAIRilocus:2096164 AT3G54340

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001994531 – 232Floral homeotic protein APETALA 3Add BLAST232

Proteomic databases

PaxDbiP35632

Expressioni

Tissue specificityi

Expressed in petals and stamens.

Inductioni

Positively regulated by the meristem identity proteins APETALA1 and LEAFY with the cooperation of UFO. Repressed by silencing mediated by polycomb group (PcG) protein complex containing EMF1 and EMF2.2 Publications

Gene expression databases

ExpressionAtlasiP35632 baseline and differential
GenevisibleiP35632 AT

Interactioni

Subunit structurei

Forms a heterodimer with PISTILLATA, capable of binding to CArG-box sequences. AP3/PI heterodimer binds AP1 or SEP3 to form complexes.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi9917, 9 interactors
IntActiP35632, 5 interactors
STRINGi3702.AT3G54340.1

Structurei

3D structure databases

ProteinModelPortaliP35632
SMRiP35632
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 57MADS-boxPROSITE-ProRule annotationAdd BLAST55
Domaini84 – 174K-boxPROSITE-ProRule annotationAdd BLAST91

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili75 – 164Sequence analysisAdd BLAST90

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0014 Eukaryota
COG5068 LUCA
InParanoidiP35632
KOiK09264
OMAiHRKYHVI
OrthoDBiEOG09360M7L
PhylomeDBiP35632

Family and domain databases

CDDicd00265 MADS_MEF2_like, 1 hit
Gene3Di3.40.1810.10, 1 hit
InterProiView protein in InterPro
IPR033896 MADS_MEF2-like
IPR002487 TF_Kbox
IPR002100 TF_MADSbox
IPR036879 TF_MADSbox_sf
PfamiView protein in Pfam
PF01486 K-box, 1 hit
PF00319 SRF-TF, 1 hit
PRINTSiPR00404 MADSDOMAIN
SMARTiView protein in SMART
SM00432 MADS, 1 hit
SUPFAMiSSF55455 SSF55455, 1 hit
PROSITEiView protein in PROSITE
PS51297 K_BOX, 1 hit
PS00350 MADS_BOX_1, 1 hit
PS50066 MADS_BOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

P35632-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGKIQIKR IENQTNRQVT YSKRRNGLFK KAHELTVLCD ARVSIIMFSS
60 70 80 90 100
SNKLHEYISP NTTTKEIVDL YQTISDVDVW ATQYERMQET KRKLLETNRN
110 120 130 140 150
LRTQIKQRLG ECLDELDIQE LRRLEDEMEN TFKLVRERKF KSLGNQIETT
160 170 180 190 200
KKKNKSQQDI QKNLIHELEL RAEDPHYGLV DNGGDYDSVL GYQIEGSRAY
210 220 230
ALRFHQNHHH YYPNHGLHAP SASDIITFHL LE
Length:232
Mass (Da):27,341
Last modified:June 1, 1994 - v1
Checksum:i669070319F9857C3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114 – 115DE → NK in AAM64919 (Ref. 7) Curated2
Sequence conflicti199A → R in BAA04665 (PubMed:7948893).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti31K → R in strain: cv. Lisse. 1 Publication1
Natural varianti47M → T in strain: cv. Bretagny. 1 Publication1
Natural varianti61N → D in strain: cv. Corsacalla-1. 1 Publication1
Natural varianti73T → S in strain: cv. Li-8. 1 Publication1
Natural varianti109L → V in strain: cv. Kas-1. 1 Publication1
Natural varianti115E → K in strain: cv. Chi-1 and cv. Gr-3. 1 Publication1
Natural varianti128M → K in strain: cv. Bla-1. 1 Publication1
Natural varianti137E → A in strain: cv. Lisse. 1 Publication1
Natural varianti141K → E in strain: cv. Lisse. 1 Publication1
Natural varianti145N → S in strain: cv. Lisse. 1 Publication1
Natural varianti151K → M in strain: cv. Kent. 1 Publication1
Natural varianti154N → S in strain: cv. Kas-1. 1 Publication1
Natural varianti159D → G in strain: cv. Kent. 1 Publication1
Natural varianti159D → N in strain: cv. Li-8. 1 Publication1
Natural varianti163N → D in strain: cv. Li-8. 1 Publication1
Natural varianti212Y → C in strain: cv. Li-8. 1 Publication1
Natural varianti219A → T in strain: cv. Bla-1. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86357 mRNA Translation: AAA32740.1
D21125 Genomic DNA Translation: BAA04665.1
AF115798 Genomic DNA Translation: AAD51887.1
AF115799 Genomic DNA Translation: AAD51888.1
AF115800 Genomic DNA Translation: AAD51889.1
AF115801 Genomic DNA Translation: AAD51890.1
AF115802 Genomic DNA Translation: AAD51891.1
AF115803 Genomic DNA Translation: AAD51892.1
AF115804 Genomic DNA Translation: AAD51893.1
AF115805 Genomic DNA Translation: AAD51894.1
AF115806 Genomic DNA Translation: AAD51895.1
AF115807 Genomic DNA Translation: AAD51896.1
AF115808 Genomic DNA Translation: AAD51897.1
AF115809 Genomic DNA Translation: AAD51898.1
AF115810 Genomic DNA Translation: AAD51899.1
AF115811 Genomic DNA Translation: AAD51900.1
AF115812 Genomic DNA Translation: AAD51901.1
AF115813 Genomic DNA Translation: AAD51902.1
AF115814 Genomic DNA Translation: AAD51903.1
AL132971 Genomic DNA Translation: CAB81799.1
CP002686 Genomic DNA Translation: AEE79216.1
AY070397 mRNA Translation: AAL49893.1
AY142590 mRNA Translation: AAN13159.1
AY087369 mRNA Translation: AAM64919.1
AF056541 Genomic DNA Translation: AAD41557.1
PIRiA42095
RefSeqiNP_191002.1, NM_115294.6
UniGeneiAt.287

Genome annotation databases

EnsemblPlantsiAT3G54340.1; AT3G54340.1; AT3G54340
GeneIDi824601
GrameneiAT3G54340.1; AT3G54340.1; AT3G54340
KEGGiath:AT3G54340

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiAP3_ARATH
AccessioniPrimary (citable) accession number: P35632
Secondary accession number(s): Q39003
, Q8LB79, Q9S7Q3, Q9SQ14, Q9SQ15, Q9SQ16, Q9SQ17, Q9SQ18, Q9SQ19, Q9SQ20, Q9SQ21, Q9SQ22, Q9SX13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 25, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

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