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Protein

Floral homeotic protein APETALA 3

Gene

AP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcription factor involved in the genetic control of flower development. Is required for normal development of petals and stamens in the wild-type flower. Forms a heterodimer with PISTILLATA that is required for autoregulation of both AP3 and PI genes. AP3/PI heterodimer interacts with APETALA1 or SEPALLATA3 to form a ternary complex that could be responsible for the regulation of the genes involved in the flower development. AP3/PI heterodimer activates the expression of NAP.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Floral homeotic protein APETALA 3
Gene namesi
Name:AP3
Ordered Locus Names:At3g54340
ORF Names:T12E18_30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G54340.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001994531 – 232Floral homeotic protein APETALA 3Add BLAST232

Proteomic databases

PaxDbiP35632.

Expressioni

Tissue specificityi

Expressed in petals and stamens.

Inductioni

Positively regulated by the meristem identity proteins APETALA1 and LEAFY with the cooperation of UFO. Repressed by silencing mediated by polycomb group (PcG) protein complex containing EMF1 and EMF2.2 Publications

Gene expression databases

GenevisibleiP35632. AT.

Interactioni

Subunit structurei

Forms a heterodimer with PISTILLATA, capable of binding to CArG-box sequences. AP3/PI heterodimer binds AP1 or SEP3 to form complexes.

Protein-protein interaction databases

BioGridi9917. 9 interactors.
IntActiP35632. 4 interactors.
STRINGi3702.AT3G54340.1.

Structurei

3D structure databases

ProteinModelPortaliP35632.
SMRiP35632.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 57MADS-boxPROSITE-ProRule annotationAdd BLAST55
Domaini84 – 174K-boxPROSITE-ProRule annotationAdd BLAST91

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili75 – 164Sequence analysisAdd BLAST90

Sequence similaritiesi

Contains 1 K-box domain.PROSITE-ProRule annotation
Contains 1 MADS-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
InParanoidiP35632.
KOiK09264.
OMAiHRKYHVI.
OrthoDBiEOG09360M7L.
PhylomeDBiP35632.

Family and domain databases

CDDicd00265. MADS_MEF2_like. 1 hit.
InterProiIPR033896. MADS_MEF2-like.
IPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35632-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGKIQIKR IENQTNRQVT YSKRRNGLFK KAHELTVLCD ARVSIIMFSS
60 70 80 90 100
SNKLHEYISP NTTTKEIVDL YQTISDVDVW ATQYERMQET KRKLLETNRN
110 120 130 140 150
LRTQIKQRLG ECLDELDIQE LRRLEDEMEN TFKLVRERKF KSLGNQIETT
160 170 180 190 200
KKKNKSQQDI QKNLIHELEL RAEDPHYGLV DNGGDYDSVL GYQIEGSRAY
210 220 230
ALRFHQNHHH YYPNHGLHAP SASDIITFHL LE
Length:232
Mass (Da):27,341
Last modified:June 1, 1994 - v1
Checksum:i669070319F9857C3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114 – 115DE → NK in AAM64919 (Ref. 7) Curated2
Sequence conflicti199A → R in BAA04665 (PubMed:7948893).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti31K → R in strain: cv. Lisse. 1 Publication1
Natural varianti47M → T in strain: cv. Bretagny. 1 Publication1
Natural varianti61N → D in strain: cv. Corsacalla-1. 1 Publication1
Natural varianti73T → S in strain: cv. Li-8. 1 Publication1
Natural varianti109L → V in strain: cv. Kas-1. 1 Publication1
Natural varianti115E → K in strain: cv. Chi-1 and cv. Gr-3. 1 Publication1
Natural varianti128M → K in strain: cv. Bla-1. 1 Publication1
Natural varianti137E → A in strain: cv. Lisse. 1 Publication1
Natural varianti141K → E in strain: cv. Lisse. 1 Publication1
Natural varianti145N → S in strain: cv. Lisse. 1 Publication1
Natural varianti151K → M in strain: cv. Kent. 1 Publication1
Natural varianti154N → S in strain: cv. Kas-1. 1 Publication1
Natural varianti159D → G in strain: cv. Kent. 1 Publication1
Natural varianti159D → N in strain: cv. Li-8. 1 Publication1
Natural varianti163N → D in strain: cv. Li-8. 1 Publication1
Natural varianti212Y → C in strain: cv. Li-8. 1 Publication1
Natural varianti219A → T in strain: cv. Bla-1. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86357 mRNA. Translation: AAA32740.1.
D21125 Genomic DNA. Translation: BAA04665.1.
AF115798 Genomic DNA. Translation: AAD51887.1.
AF115799 Genomic DNA. Translation: AAD51888.1.
AF115800 Genomic DNA. Translation: AAD51889.1.
AF115801 Genomic DNA. Translation: AAD51890.1.
AF115802 Genomic DNA. Translation: AAD51891.1.
AF115803 Genomic DNA. Translation: AAD51892.1.
AF115804 Genomic DNA. Translation: AAD51893.1.
AF115805 Genomic DNA. Translation: AAD51894.1.
AF115806 Genomic DNA. Translation: AAD51895.1.
AF115807 Genomic DNA. Translation: AAD51896.1.
AF115808 Genomic DNA. Translation: AAD51897.1.
AF115809 Genomic DNA. Translation: AAD51898.1.
AF115810 Genomic DNA. Translation: AAD51899.1.
AF115811 Genomic DNA. Translation: AAD51900.1.
AF115812 Genomic DNA. Translation: AAD51901.1.
AF115813 Genomic DNA. Translation: AAD51902.1.
AF115814 Genomic DNA. Translation: AAD51903.1.
AL132971 Genomic DNA. Translation: CAB81799.1.
CP002686 Genomic DNA. Translation: AEE79216.1.
AY070397 mRNA. Translation: AAL49893.1.
AY142590 mRNA. Translation: AAN13159.1.
AY087369 mRNA. Translation: AAM64919.1.
AF056541 Genomic DNA. Translation: AAD41557.1.
PIRiA42095.
RefSeqiNP_191002.1. NM_115294.6.
UniGeneiAt.287.

Genome annotation databases

EnsemblPlantsiAT3G54340.1; AT3G54340.1; AT3G54340.
GeneIDi824601.
GrameneiAT3G54340.1; AT3G54340.1; AT3G54340.
KEGGiath:AT3G54340.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86357 mRNA. Translation: AAA32740.1.
D21125 Genomic DNA. Translation: BAA04665.1.
AF115798 Genomic DNA. Translation: AAD51887.1.
AF115799 Genomic DNA. Translation: AAD51888.1.
AF115800 Genomic DNA. Translation: AAD51889.1.
AF115801 Genomic DNA. Translation: AAD51890.1.
AF115802 Genomic DNA. Translation: AAD51891.1.
AF115803 Genomic DNA. Translation: AAD51892.1.
AF115804 Genomic DNA. Translation: AAD51893.1.
AF115805 Genomic DNA. Translation: AAD51894.1.
AF115806 Genomic DNA. Translation: AAD51895.1.
AF115807 Genomic DNA. Translation: AAD51896.1.
AF115808 Genomic DNA. Translation: AAD51897.1.
AF115809 Genomic DNA. Translation: AAD51898.1.
AF115810 Genomic DNA. Translation: AAD51899.1.
AF115811 Genomic DNA. Translation: AAD51900.1.
AF115812 Genomic DNA. Translation: AAD51901.1.
AF115813 Genomic DNA. Translation: AAD51902.1.
AF115814 Genomic DNA. Translation: AAD51903.1.
AL132971 Genomic DNA. Translation: CAB81799.1.
CP002686 Genomic DNA. Translation: AEE79216.1.
AY070397 mRNA. Translation: AAL49893.1.
AY142590 mRNA. Translation: AAN13159.1.
AY087369 mRNA. Translation: AAM64919.1.
AF056541 Genomic DNA. Translation: AAD41557.1.
PIRiA42095.
RefSeqiNP_191002.1. NM_115294.6.
UniGeneiAt.287.

3D structure databases

ProteinModelPortaliP35632.
SMRiP35632.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9917. 9 interactors.
IntActiP35632. 4 interactors.
STRINGi3702.AT3G54340.1.

Proteomic databases

PaxDbiP35632.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G54340.1; AT3G54340.1; AT3G54340.
GeneIDi824601.
GrameneiAT3G54340.1; AT3G54340.1; AT3G54340.
KEGGiath:AT3G54340.

Organism-specific databases

TAIRiAT3G54340.

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
InParanoidiP35632.
KOiK09264.
OMAiHRKYHVI.
OrthoDBiEOG09360M7L.
PhylomeDBiP35632.

Miscellaneous databases

PROiP35632.

Gene expression databases

GenevisibleiP35632. AT.

Family and domain databases

CDDicd00265. MADS_MEF2_like. 1 hit.
InterProiIPR033896. MADS_MEF2-like.
IPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAP3_ARATH
AccessioniPrimary (citable) accession number: P35632
Secondary accession number(s): Q39003
, Q8LB79, Q9S7Q3, Q9SQ14, Q9SQ15, Q9SQ16, Q9SQ17, Q9SQ18, Q9SQ19, Q9SQ20, Q9SQ21, Q9SQ22, Q9SX13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutations in AP3 cause transformation of petals into sepals and stamina into carpels.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.