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Protein

Floral homeotic protein APETALA 3

Gene

AP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcription factor involved in the genetic control of flower development. Is required for normal development of petals and stamens in the wild-type flower. Forms a heterodimer with PISTILLATA that is required for autoregulation of both AP3 and PI genes. AP3/PI heterodimer interacts with APETALA1 or SEPALLATA3 to form a ternary complex that could be responsible for the regulation of the genes involved in the flower development. AP3/PI heterodimer activates the expression of NAP.2 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • specification of floral organ identity Source: CACAO
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Floral homeotic protein APETALA 3
Gene namesi
Name:AP3
Ordered Locus Names:At3g54340
ORF Names:T12E18_30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G54340.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232Floral homeotic protein APETALA 3PRO_0000199453Add
BLAST

Proteomic databases

PaxDbiP35632.
PRIDEiP35632.

Expressioni

Tissue specificityi

Expressed in petals and stamens.

Inductioni

Positively regulated by the meristem identity proteins APETALA1 and LEAFY with the cooperation of UFO. Repressed by silencing mediated by polycomb group (PcG) protein complex containing EMF1 and EMF2.2 Publications

Gene expression databases

GenevisibleiP35632. AT.

Interactioni

Subunit structurei

Forms a heterodimer with PISTILLATA, capable of binding to CArG-box sequences. AP3/PI heterodimer binds AP1 or SEP3 to form complexes.

Protein-protein interaction databases

BioGridi9917. 9 interactions.
IntActiP35632. 4 interactions.
STRINGi3702.AT3G54340.1.

Structurei

3D structure databases

ProteinModelPortaliP35632.
SMRiP35632. Positions 2-93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 5755MADS-boxPROSITE-ProRule annotationAdd
BLAST
Domaini84 – 17491K-boxPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili75 – 16490Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 K-box domain.PROSITE-ProRule annotation
Contains 1 MADS-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
InParanoidiP35632.
KOiK09264.
OMAiHRKYHVI.
OrthoDBiEOG09360M7L.
PhylomeDBiP35632.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35632-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGKIQIKR IENQTNRQVT YSKRRNGLFK KAHELTVLCD ARVSIIMFSS
60 70 80 90 100
SNKLHEYISP NTTTKEIVDL YQTISDVDVW ATQYERMQET KRKLLETNRN
110 120 130 140 150
LRTQIKQRLG ECLDELDIQE LRRLEDEMEN TFKLVRERKF KSLGNQIETT
160 170 180 190 200
KKKNKSQQDI QKNLIHELEL RAEDPHYGLV DNGGDYDSVL GYQIEGSRAY
210 220 230
ALRFHQNHHH YYPNHGLHAP SASDIITFHL LE
Length:232
Mass (Da):27,341
Last modified:June 1, 1994 - v1
Checksum:i669070319F9857C3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1152DE → NK in AAM64919 (Ref. 7) Curated
Sequence conflicti199 – 1991A → R in BAA04665 (PubMed:7948893).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti31 – 311K → R in strain: cv. Lisse. 1 Publication
Natural varianti47 – 471M → T in strain: cv. Bretagny. 1 Publication
Natural varianti61 – 611N → D in strain: cv. Corsacalla-1. 1 Publication
Natural varianti73 – 731T → S in strain: cv. Li-8. 1 Publication
Natural varianti109 – 1091L → V in strain: cv. Kas-1. 1 Publication
Natural varianti115 – 1151E → K in strain: cv. Chi-1 and cv. Gr-3. 1 Publication
Natural varianti128 – 1281M → K in strain: cv. Bla-1. 1 Publication
Natural varianti137 – 1371E → A in strain: cv. Lisse. 1 Publication
Natural varianti141 – 1411K → E in strain: cv. Lisse. 1 Publication
Natural varianti145 – 1451N → S in strain: cv. Lisse. 1 Publication
Natural varianti151 – 1511K → M in strain: cv. Kent. 1 Publication
Natural varianti154 – 1541N → S in strain: cv. Kas-1. 1 Publication
Natural varianti159 – 1591D → G in strain: cv. Kent. 1 Publication
Natural varianti159 – 1591D → N in strain: cv. Li-8. 1 Publication
Natural varianti163 – 1631N → D in strain: cv. Li-8. 1 Publication
Natural varianti212 – 2121Y → C in strain: cv. Li-8. 1 Publication
Natural varianti219 – 2191A → T in strain: cv. Bla-1. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86357 mRNA. Translation: AAA32740.1.
D21125 Genomic DNA. Translation: BAA04665.1.
AF115798 Genomic DNA. Translation: AAD51887.1.
AF115799 Genomic DNA. Translation: AAD51888.1.
AF115800 Genomic DNA. Translation: AAD51889.1.
AF115801 Genomic DNA. Translation: AAD51890.1.
AF115802 Genomic DNA. Translation: AAD51891.1.
AF115803 Genomic DNA. Translation: AAD51892.1.
AF115804 Genomic DNA. Translation: AAD51893.1.
AF115805 Genomic DNA. Translation: AAD51894.1.
AF115806 Genomic DNA. Translation: AAD51895.1.
AF115807 Genomic DNA. Translation: AAD51896.1.
AF115808 Genomic DNA. Translation: AAD51897.1.
AF115809 Genomic DNA. Translation: AAD51898.1.
AF115810 Genomic DNA. Translation: AAD51899.1.
AF115811 Genomic DNA. Translation: AAD51900.1.
AF115812 Genomic DNA. Translation: AAD51901.1.
AF115813 Genomic DNA. Translation: AAD51902.1.
AF115814 Genomic DNA. Translation: AAD51903.1.
AL132971 Genomic DNA. Translation: CAB81799.1.
CP002686 Genomic DNA. Translation: AEE79216.1.
AY070397 mRNA. Translation: AAL49893.1.
AY142590 mRNA. Translation: AAN13159.1.
AY087369 mRNA. Translation: AAM64919.1.
AF056541 Genomic DNA. Translation: AAD41557.1.
PIRiA42095.
RefSeqiNP_191002.1. NM_115294.5.
UniGeneiAt.287.

Genome annotation databases

EnsemblPlantsiAT3G54340.1; AT3G54340.1; AT3G54340.
GeneIDi824601.
GrameneiAT3G54340.1; AT3G54340.1; AT3G54340.
KEGGiath:AT3G54340.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86357 mRNA. Translation: AAA32740.1.
D21125 Genomic DNA. Translation: BAA04665.1.
AF115798 Genomic DNA. Translation: AAD51887.1.
AF115799 Genomic DNA. Translation: AAD51888.1.
AF115800 Genomic DNA. Translation: AAD51889.1.
AF115801 Genomic DNA. Translation: AAD51890.1.
AF115802 Genomic DNA. Translation: AAD51891.1.
AF115803 Genomic DNA. Translation: AAD51892.1.
AF115804 Genomic DNA. Translation: AAD51893.1.
AF115805 Genomic DNA. Translation: AAD51894.1.
AF115806 Genomic DNA. Translation: AAD51895.1.
AF115807 Genomic DNA. Translation: AAD51896.1.
AF115808 Genomic DNA. Translation: AAD51897.1.
AF115809 Genomic DNA. Translation: AAD51898.1.
AF115810 Genomic DNA. Translation: AAD51899.1.
AF115811 Genomic DNA. Translation: AAD51900.1.
AF115812 Genomic DNA. Translation: AAD51901.1.
AF115813 Genomic DNA. Translation: AAD51902.1.
AF115814 Genomic DNA. Translation: AAD51903.1.
AL132971 Genomic DNA. Translation: CAB81799.1.
CP002686 Genomic DNA. Translation: AEE79216.1.
AY070397 mRNA. Translation: AAL49893.1.
AY142590 mRNA. Translation: AAN13159.1.
AY087369 mRNA. Translation: AAM64919.1.
AF056541 Genomic DNA. Translation: AAD41557.1.
PIRiA42095.
RefSeqiNP_191002.1. NM_115294.5.
UniGeneiAt.287.

3D structure databases

ProteinModelPortaliP35632.
SMRiP35632. Positions 2-93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9917. 9 interactions.
IntActiP35632. 4 interactions.
STRINGi3702.AT3G54340.1.

Proteomic databases

PaxDbiP35632.
PRIDEiP35632.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G54340.1; AT3G54340.1; AT3G54340.
GeneIDi824601.
GrameneiAT3G54340.1; AT3G54340.1; AT3G54340.
KEGGiath:AT3G54340.

Organism-specific databases

TAIRiAT3G54340.

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
InParanoidiP35632.
KOiK09264.
OMAiHRKYHVI.
OrthoDBiEOG09360M7L.
PhylomeDBiP35632.

Miscellaneous databases

PROiP35632.

Gene expression databases

GenevisibleiP35632. AT.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAP3_ARATH
AccessioniPrimary (citable) accession number: P35632
Secondary accession number(s): Q39003
, Q8LB79, Q9S7Q3, Q9SQ14, Q9SQ15, Q9SQ16, Q9SQ17, Q9SQ18, Q9SQ19, Q9SQ20, Q9SQ21, Q9SQ22, Q9SX13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutations in AP3 cause transformation of petals into sepals and stamina into carpels.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.