Reviewed, UniProtKB/Swiss-Prot P35630 (ADH1_ENTHI)

Names and origin

Protein names

Recommended name:
    NADP-dependent alcohol dehydrogenase
    EC=1.1.1.2

Gene names

Name:

ADH1

Organism

Entamoeba histolytica

Taxonomic identifier

5759 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Archamoebae › Entamoebidae › Entamoeba

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	An alcohol + NADP+ = an aldehyde + NADPH.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer. Ref.3
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NADP Zinc
Molecular function	Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alcohol dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 360

360

NADP-dependent alcohol dehydrogenase

PRO_0000160695

Regions

Nucleotide binding

174 – 179

6

NAD By similarity

Nucleotide binding

265 – 267

3

NAD By similarity

Sites

Metal binding

37

1

Zinc; catalytic

Metal binding

59

1

Zinc; catalytic

Metal binding

60

1

Zinc; catalytic

Metal binding

150

1

Zinc; catalytic

Secondary structure

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360

Helix Strand Turn

Details...

Beta strand

2 – 8

7

Beta strand

11 – 16

6

Beta strand

27 – 34

8

Helix

38 – 45

8

Beta strand

53 – 56

4

Beta strand

61 – 68

8

Beta strand

80 – 83

4

Helix

93 – 96

4

Helix

100 – 102

3

Turn

106 – 109

4

Turn

113 – 115

3

Beta strand

119 – 128

10

Helix

129 – 132

4

Helix

142 – 145

4

Helix

146 – 148

3

Helix

151 – 161

11

Beta strand

170 – 173

4

Helix

177 – 188

12

Beta strand

192 – 197

6

Helix

201 – 209

9

Beta strand

214 – 216

3

Helix

218 – 220

3

Helix

223 – 230

8

Turn

231 – 233

3

Beta strand

236 – 241

6

Helix

248 – 255

8

Beta strand

256 – 264

9

Beta strand

272 – 277

6

Turn

278 – 281

4

Helix

282 – 284

3

Beta strand

288 – 293

6

Helix

298 – 310

13

Helix

317 – 319

3

Beta strand

320 – 326

7

Helix

329 – 339

11

Beta strand

345 – 350

6

Turn

354 – 358

5

Sequences

	Sequence		Length	Mass (Da)
	P35630-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 6D01643EB4A3A07F	FASTA	360	38,568
	10 20 30 40 50 60 MKGLAMLGIG RIGWIEKKIP ECGPLDALVR PLALAPCTSD THTVWAGAIG DRHDMILGHE 70 80 90 100 110 120 AVGQIVKVGS LVKRLKVGDK VIVPAITPDW GEEESQRGYP MHSGGMLGGW KFSNFKDGVF 130 140 150 160 170 180 SEVFHVNEAD ANLALLPRDI KPEDAVMLSD MVTTGFHGAE LANIKLGDTV CVIGIGPVGL 190 200 210 220 230 240 MSVAGANHLG AGRIFAVGSR KHCCDIALEY GATDIINYKN GDIVEQILKA TDGKGVDKVV 250 260 270 280 290 300 IAGGDVHTFA QAVKMIKPGS DIGNVNYLGE GDNIDIPRSE WGVGMGHKHI HGGLTPGGRV 310 320 330 340 350 360 RMEKLASLIS TGKLDTSKLI THRFEGLEKV EDALMLMKNK PADLIKPVVR IHYDDEDTLH

References

[1]	"Cloning and expression of an NADP(+)-dependent alcohol dehydrogenase gene of Entamoeba histolytica." Kumar A., Shen P.S., Descoteaux S., Pohl J., Bailey G., Samuelson J. Proc. Natl. Acad. Sci. U.S.A. 89:10188-10192(1992) [PubMed: 1438208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Primary structures of alcohol and aldehyde dehydrogenase genes of Entamoeba histolytica." Samuelson J., Zhang W.W., Kumar A., Descoteaux S., Shen P.S., Bailey G. Arch. Med. Res. 23:31-33(1992) [PubMed: 1340318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structure of alcohol dehydrogenase from Entamoeba histolytica." Shimon L.J.W., Goihberg E., Peretz M., Burstein Y., Frolow F. Acta Crystallogr. D 62:541-547(2006) [PubMed: 16627948] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.81 ANGSTROMS) IN COMPLEX WITH ZINC IONS, SUBUNIT.
[4]	"Thermal stabilization of the protozoan Entamoeba histolytica alcohol dehydrogenase by a single proline substitution." Protein structure factory (PSF) Submitted (FEB-2008) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.77 ANGSTROMS) IN COMPLEX WITH ZINC IONS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M88600 mRNA. Translation: AAA51479.1.

PIR

A46409.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Y9A	X-ray	1.81	A/C	1-360	[»]
2OUI	X-ray	1.77	A/B/C/D	1-360	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

1.1.1.2. 323.

Family and domain databases

InterPro

IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn.
IPR013149. ADH_Zn-bd.
IPR002328. ADH_Zn_CS.
[Graphical view]

PANTHER

PTHR11695. ADH_Sf_Zn. 1 hit.

Pfam

PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]

PROSITE

PS00059. ADH_ZINC. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ADH1_ENTHI

Accession

Primary (citable) accession number: P35630

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)