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Protein

NADP-dependent isopropanol dehydrogenase

Gene

ADH1

Organism
Entamoeba histolytica
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Alcohol dehydrogenase with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Has very low activity with primary alcohols, such as ethanol. Under physiological conditions, the enzyme reduces aldehydes and 2-ketones to produce secondary alcohols. Is also active with acetaldehyde and propionaldehyde.1 Publication

Catalytic activityi

Propan-2-ol + NADP+ = acetone + NADPH.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi37Zinc; catalytic1 Publication1
Metal bindingi59Zinc; catalytic1 Publication1
Metal bindingi60Zinc; catalytic1 Publication1
Metal bindingi150Zinc; catalytic1 Publication1
Binding sitei218NADPBy similarity1
Binding sitei340NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi175 – 178NADPBy similarity4
Nucleotide bindingi198 – 200NADPBy similarity3
Nucleotide bindingi265 – 267NADPBy similarity3

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NADP, Zinc

Enzyme and pathway databases

BRENDAi1.1.1.2. 2080.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-dependent isopropanol dehydrogenase (EC:1.1.1.80)
Gene namesi
Name:ADH1
OrganismiEntamoeba histolytica
Taxonomic identifieri5759 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001606951 – 360NADP-dependent isopropanol dehydrogenaseAdd BLAST360

Interactioni

Subunit structurei

Homodimer.3 Publications

Protein-protein interaction databases

STRINGi5759.rna_EHI_023110-1.

Structurei

Secondary structure

1360
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Beta strandi11 – 16Combined sources6
Beta strandi27 – 34Combined sources8
Helixi38 – 45Combined sources8
Beta strandi53 – 56Combined sources4
Beta strandi61 – 68Combined sources8
Beta strandi80 – 83Combined sources4
Helixi93 – 96Combined sources4
Helixi100 – 102Combined sources3
Turni106 – 109Combined sources4
Turni113 – 115Combined sources3
Beta strandi119 – 128Combined sources10
Helixi129 – 132Combined sources4
Helixi142 – 145Combined sources4
Helixi146 – 148Combined sources3
Helixi153 – 161Combined sources9
Beta strandi170 – 173Combined sources4
Helixi177 – 187Combined sources11
Turni188 – 190Combined sources3
Beta strandi192 – 197Combined sources6
Helixi201 – 210Combined sources10
Beta strandi214 – 216Combined sources3
Helixi218 – 220Combined sources3
Helixi223 – 230Combined sources8
Turni231 – 233Combined sources3
Beta strandi236 – 241Combined sources6
Helixi248 – 255Combined sources8
Beta strandi256 – 264Combined sources9
Beta strandi272 – 277Combined sources6
Turni278 – 281Combined sources4
Helixi282 – 284Combined sources3
Beta strandi288 – 293Combined sources6
Helixi298 – 310Combined sources13
Helixi317 – 319Combined sources3
Beta strandi320 – 326Combined sources7
Helixi329 – 339Combined sources11
Beta strandi345 – 350Combined sources6
Turni354 – 358Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y9AX-ray1.81A/C1-360[»]
2OUIX-ray1.77A/B/C/D1-360[»]
3FPCX-ray1.40A/B/C/D153-294[»]
ProteinModelPortaliP35630.
SMRiP35630.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35630.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0024. Eukaryota.
COG1063. LUCA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35630-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGLAMLGIG RIGWIEKKIP ECGPLDALVR PLALAPCTSD THTVWAGAIG
60 70 80 90 100
DRHDMILGHE AVGQIVKVGS LVKRLKVGDK VIVPAITPDW GEEESQRGYP
110 120 130 140 150
MHSGGMLGGW KFSNFKDGVF SEVFHVNEAD ANLALLPRDI KPEDAVMLSD
160 170 180 190 200
MVTTGFHGAE LANIKLGDTV CVIGIGPVGL MSVAGANHLG AGRIFAVGSR
210 220 230 240 250
KHCCDIALEY GATDIINYKN GDIVEQILKA TDGKGVDKVV IAGGDVHTFA
260 270 280 290 300
QAVKMIKPGS DIGNVNYLGE GDNIDIPRSE WGVGMGHKHI HGGLTPGGRV
310 320 330 340 350
RMEKLASLIS TGKLDTSKLI THRFEGLEKV EDALMLMKNK PADLIKPVVR
360
IHYDDEDTLH
Length:360
Mass (Da):38,568
Last modified:June 1, 1994 - v1
Checksum:i6D01643EB4A3A07F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88600 mRNA. Translation: AAA51479.1.
PIRiA46409.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88600 mRNA. Translation: AAA51479.1.
PIRiA46409.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y9AX-ray1.81A/C1-360[»]
2OUIX-ray1.77A/B/C/D1-360[»]
3FPCX-ray1.40A/B/C/D153-294[»]
ProteinModelPortaliP35630.
SMRiP35630.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5759.rna_EHI_023110-1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0024. Eukaryota.
COG1063. LUCA.

Enzyme and pathway databases

BRENDAi1.1.1.2. 2080.

Miscellaneous databases

EvolutionaryTraceiP35630.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADH1_ENTHI
AccessioniPrimary (citable) accession number: P35630
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.