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Protein

Beta-adrenergic receptor kinase 2

Gene

GRK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.

Catalytic activityi

ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATPPROSITE-ProRule annotation1
Active sitei317Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 205ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • receptor internalization Source: UniProtKB
  • signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS01969-MONOMER.
BRENDAi2.7.11.15. 2681.
ReactomeiR-HSA-418555. G alpha (s) signalling events.
R-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.
SignaLinkiP35626.
SIGNORiP35626.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-adrenergic receptor kinase 2 (EC:2.7.11.15)
Short name:
Beta-ARK-2
Alternative name(s):
G-protein-coupled receptor kinase 3Imported
Gene namesi
Name:GRK3Imported
Synonyms:ADRBK2, BARK2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:290. GRK3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi157.
OpenTargetsiENSG00000100077.
PharmGKBiPA41.

Chemistry databases

ChEMBLiCHEMBL1075166.
DrugBankiDB00171. Adenosine triphosphate.
GuidetoPHARMACOLOGYi1467.

Polymorphism and mutation databases

BioMutaiADRBK2.
DMDMi116241253.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000856321 – 688Beta-adrenergic receptor kinase 2Add BLAST688

Post-translational modificationi

Ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiP35626.
MaxQBiP35626.
PaxDbiP35626.
PeptideAtlasiP35626.
PRIDEiP35626.

PTM databases

iPTMnetiP35626.
PhosphoSitePlusiP35626.

Expressioni

Gene expression databases

BgeeiENSG00000100077.
CleanExiHS_ADRBK2.
ExpressionAtlasiP35626. baseline and differential.
GenevisibleiP35626. HS.

Organism-specific databases

HPAiHPA000804.

Interactioni

Protein-protein interaction databases

BioGridi106666. 7 interactors.
IntActiP35626. 4 interactors.
STRINGi9606.ENSP00000317578.

Chemistry databases

BindingDBiP35626.

Structurei

3D structure databases

ProteinModelPortaliP35626.
SMRiP35626.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 175RGSPROSITE-ProRule annotationAdd BLAST122
Domaini191 – 453Protein kinasePROSITE-ProRule annotationAdd BLAST263
Domaini454 – 521AGC-kinase C-terminalAdd BLAST68
Domaini558 – 652PHPROSITE-ProRule annotationAdd BLAST95

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 190N-terminalAdd BLAST190

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0986. Eukaryota.
ENOG410YRQZ. LUCA.
GeneTreeiENSGT00860000133699.
HOGENOMiHOG000006742.
HOVERGENiHBG050559.
InParanoidiP35626.
KOiK00910.
OMAiDHVQTHL.
OrthoDBiEOG091G0AEI.
PhylomeDBiP35626.
TreeFamiTF313940.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00233. PH. 1 hit.
SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35626-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLEAVLAD VSYLMAMEKS KATPAARASK RIVLPEPSIR SVMQKYLAER
60 70 80 90 100
NEITFDKIFN QKIGFLLFKD FCLNEINEAV PQVKFYEEIK EYEKLDNEED
110 120 130 140 150
RLCRSRQIYD AYIMKELLSC SHPFSKQAVE HVQSHLSKKQ VTSTLFQPYI
160 170 180 190 200
EEICESLRGD IFQKFMESDK FTRFCQWKNV ELNIHLTMNE FSVHRIIGRG
210 220 230 240 250
GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER IMLSLVSTGD
260 270 280 290 300
CPFIVCMTYA FHTPDKLCFI LDLMNGGDLH YHLSQHGVFS EKEMRFYATE
310 320 330 340 350
IILGLEHMHN RFVVYRDLKP ANILLDEHGH ARISDLGLAC DFSKKKPHAS
360 370 380 390 400
VGTHGYMAPE VLQKGTAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH
410 420 430 440 450
EIDRMTLTVN VELPDTFSPE LKSLLEGLLQ RDVSKRLGCH GGGSQEVKEH
460 470 480 490 500
SFFKGVDWQH VYLQKYPPPL IPPRGEVNAA DAFDIGSFDE EDTKGIKLLD
510 520 530 540 550
CDQELYKNFP LVISERWQQE VTETVYEAVN ADTDKIEARK RAKNKQLGHE
560 570 580 590 600
EDYALGKDCI MHGYMLKLGN PFLTQWQRRY FYLFPNRLEW RGEGESRQNL
610 620 630 640 650
LTMEQILSVE ETQIKDKKCI LFRIKGGKQF VLQCESDPEF VQWKKELNET
660 670 680
FKEAQRLLRR APKFLNKPRS GTVELPKPSL CHRNSNGL
Length:688
Mass (Da):79,710
Last modified:October 17, 2006 - v2
Checksum:i50844236A01C1423
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti308M → V in CAA48870 (PubMed:8427589).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04038050R → S.1 PublicationCorresponds to variant rs55700971dbSNPEnsembl.1
Natural variantiVAR_04038160N → S.1 PublicationCorresponds to variant rs55740593dbSNPEnsembl.1
Natural variantiVAR_040382104R → K in a lung bronchoalveolar carcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_028005409V → M.Corresponds to variant rs2272859dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69117 mRNA. Translation: CAA48870.1.
AL022329 Genomic DNA. Translation: CAB45657.1.
CCDSiCCDS13832.1.
PIRiJC1469.
RefSeqiNP_005151.2. NM_005160.3.
UniGeneiHs.657494.
Hs.733960.

Genome annotation databases

EnsembliENST00000324198; ENSP00000317578; ENSG00000100077.
GeneIDi157.
KEGGihsa:157.
UCSCiuc003abx.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69117 mRNA. Translation: CAA48870.1.
AL022329 Genomic DNA. Translation: CAB45657.1.
CCDSiCCDS13832.1.
PIRiJC1469.
RefSeqiNP_005151.2. NM_005160.3.
UniGeneiHs.657494.
Hs.733960.

3D structure databases

ProteinModelPortaliP35626.
SMRiP35626.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106666. 7 interactors.
IntActiP35626. 4 interactors.
STRINGi9606.ENSP00000317578.

Chemistry databases

BindingDBiP35626.
ChEMBLiCHEMBL1075166.
DrugBankiDB00171. Adenosine triphosphate.
GuidetoPHARMACOLOGYi1467.

PTM databases

iPTMnetiP35626.
PhosphoSitePlusiP35626.

Polymorphism and mutation databases

BioMutaiADRBK2.
DMDMi116241253.

Proteomic databases

EPDiP35626.
MaxQBiP35626.
PaxDbiP35626.
PeptideAtlasiP35626.
PRIDEiP35626.

Protocols and materials databases

DNASUi157.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000324198; ENSP00000317578; ENSG00000100077.
GeneIDi157.
KEGGihsa:157.
UCSCiuc003abx.5. human.

Organism-specific databases

CTDi157.
DisGeNETi157.
GeneCardsiADRBK2.
H-InvDBHIX0041309.
HGNCiHGNC:290. GRK3.
HPAiHPA000804.
MIMi109636. gene.
neXtProtiNX_P35626.
OpenTargetsiENSG00000100077.
PharmGKBiPA41.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0986. Eukaryota.
ENOG410YRQZ. LUCA.
GeneTreeiENSGT00860000133699.
HOGENOMiHOG000006742.
HOVERGENiHBG050559.
InParanoidiP35626.
KOiK00910.
OMAiDHVQTHL.
OrthoDBiEOG091G0AEI.
PhylomeDBiP35626.
TreeFamiTF313940.

Enzyme and pathway databases

BioCyciZFISH:HS01969-MONOMER.
BRENDAi2.7.11.15. 2681.
ReactomeiR-HSA-418555. G alpha (s) signalling events.
R-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.
SignaLinkiP35626.
SIGNORiP35626.

Miscellaneous databases

ChiTaRSiADRBK2. human.
GeneWikiiBeta_adrenergic_receptor_kinase-2.
GenomeRNAii157.
PROiP35626.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100077.
CleanExiHS_ADRBK2.
ExpressionAtlasiP35626. baseline and differential.
GenevisibleiP35626. HS.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00233. PH. 1 hit.
SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARBK2_HUMAN
AccessioniPrimary (citable) accession number: P35626
Secondary accession number(s): Q9UGW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 17, 2006
Last modified: November 30, 2016
This is version 166 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.