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P35626

- ARBK2_HUMAN

UniProt

P35626 - ARBK2_HUMAN

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Protein
Beta-adrenergic receptor kinase 2
Gene
ADRBK2, BARK2, GRK3
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.

Catalytic activityi

ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei220 – 2201ATP By similarity
Active sitei317 – 3171Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi197 – 2059ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. G-protein coupled receptor kinase activity Source: Ensembl
  3. beta-adrenergic receptor kinase activity Source: UniProtKB-EC
  4. protein kinase activity Source: ProtInc

GO - Biological processi

  1. receptor internalization Source: UniProtKB
  2. signal transduction Source: ProtInc
  3. termination of G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.15. 2681.
SignaLinkiP35626.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-adrenergic receptor kinase 2 (EC:2.7.11.15)
Short name:
Beta-ARK-2
Alternative name(s):
G-protein-coupled receptor kinase 3
Gene namesi
Name:ADRBK2
Synonyms:BARK2, GRK3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 22

Organism-specific databases

HGNCiHGNC:290. ADRBK2.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA41.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 688688Beta-adrenergic receptor kinase 2
PRO_0000085632Add
BLAST

Post-translational modificationi

Ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiP35626.
PaxDbiP35626.
PRIDEiP35626.

PTM databases

PhosphoSiteiP35626.

Expressioni

Gene expression databases

ArrayExpressiP35626.
BgeeiP35626.
CleanExiHS_ADRBK2.
GenevestigatoriP35626.

Organism-specific databases

HPAiHPA000804.

Interactioni

Protein-protein interaction databases

BioGridi106666. 6 interactions.
IntActiP35626. 3 interactions.
STRINGi9606.ENSP00000317578.

Structurei

3D structure databases

ProteinModelPortaliP35626.
SMRiP35626. Positions 30-668.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 175122RGS
Add
BLAST
Domaini191 – 453263Protein kinase
Add
BLAST
Domaini454 – 52168AGC-kinase C-terminal
Add
BLAST
Domaini558 – 65295PH
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 190190N-terminal
Add
BLAST

Sequence similaritiesi

Contains 1 PH domain.
Contains 1 RGS domain.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000006742.
HOVERGENiHBG050559.
InParanoidiP35626.
KOiK00910.
OMAiQTHLAKK.
OrthoDBiEOG7FV3PP.
PhylomeDBiP35626.
TreeFamiTF313940.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00233. PH. 1 hit.
SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35626-1 [UniParc]FASTAAdd to Basket

« Hide

MADLEAVLAD VSYLMAMEKS KATPAARASK RIVLPEPSIR SVMQKYLAER    50
NEITFDKIFN QKIGFLLFKD FCLNEINEAV PQVKFYEEIK EYEKLDNEED 100
RLCRSRQIYD AYIMKELLSC SHPFSKQAVE HVQSHLSKKQ VTSTLFQPYI 150
EEICESLRGD IFQKFMESDK FTRFCQWKNV ELNIHLTMNE FSVHRIIGRG 200
GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER IMLSLVSTGD 250
CPFIVCMTYA FHTPDKLCFI LDLMNGGDLH YHLSQHGVFS EKEMRFYATE 300
IILGLEHMHN RFVVYRDLKP ANILLDEHGH ARISDLGLAC DFSKKKPHAS 350
VGTHGYMAPE VLQKGTAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH 400
EIDRMTLTVN VELPDTFSPE LKSLLEGLLQ RDVSKRLGCH GGGSQEVKEH 450
SFFKGVDWQH VYLQKYPPPL IPPRGEVNAA DAFDIGSFDE EDTKGIKLLD 500
CDQELYKNFP LVISERWQQE VTETVYEAVN ADTDKIEARK RAKNKQLGHE 550
EDYALGKDCI MHGYMLKLGN PFLTQWQRRY FYLFPNRLEW RGEGESRQNL 600
LTMEQILSVE ETQIKDKKCI LFRIKGGKQF VLQCESDPEF VQWKKELNET 650
FKEAQRLLRR APKFLNKPRS GTVELPKPSL CHRNSNGL 688
Length:688
Mass (Da):79,710
Last modified:October 17, 2006 - v2
Checksum:i50844236A01C1423
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti50 – 501R → S.1 Publication
Corresponds to variant rs55700971 [ dbSNP | Ensembl ].
VAR_040380
Natural varianti60 – 601N → S.1 Publication
Corresponds to variant rs55740593 [ dbSNP | Ensembl ].
VAR_040381
Natural varianti104 – 1041R → K in a lung bronchoalveolar carcinoma sample; somatic mutation. 1 Publication
VAR_040382
Natural varianti409 – 4091V → M.
Corresponds to variant rs2272859 [ dbSNP | Ensembl ].
VAR_028005

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti308 – 3081M → V in CAA48870. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69117 mRNA. Translation: CAA48870.1.
AL022329 Genomic DNA. Translation: CAB45657.1.
CCDSiCCDS13832.1.
PIRiJC1469.
RefSeqiNP_005151.2. NM_005160.3.
UniGeneiHs.657494.

Genome annotation databases

EnsembliENST00000324198; ENSP00000317578; ENSG00000100077.
GeneIDi157.
KEGGihsa:157.
UCSCiuc003abw.2. human.

Polymorphism databases

DMDMi116241253.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69117 mRNA. Translation: CAA48870.1 .
AL022329 Genomic DNA. Translation: CAB45657.1 .
CCDSi CCDS13832.1.
PIRi JC1469.
RefSeqi NP_005151.2. NM_005160.3.
UniGenei Hs.657494.

3D structure databases

ProteinModelPortali P35626.
SMRi P35626. Positions 30-668.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 106666. 6 interactions.
IntActi P35626. 3 interactions.
STRINGi 9606.ENSP00000317578.

Chemistry

BindingDBi P35626.
ChEMBLi CHEMBL1075166.
DrugBanki DB00171. Adenosine triphosphate.
GuidetoPHARMACOLOGYi 1467.

PTM databases

PhosphoSitei P35626.

Polymorphism databases

DMDMi 116241253.

Proteomic databases

MaxQBi P35626.
PaxDbi P35626.
PRIDEi P35626.

Protocols and materials databases

DNASUi 157.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000324198 ; ENSP00000317578 ; ENSG00000100077 .
GeneIDi 157.
KEGGi hsa:157.
UCSCi uc003abw.2. human.

Organism-specific databases

CTDi 157.
GeneCardsi GC22P025960.
H-InvDB HIX0041309.
HGNCi HGNC:290. ADRBK2.
HPAi HPA000804.
MIMi 109636. gene.
neXtProti NX_P35626.
PharmGKBi PA41.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000006742.
HOVERGENi HBG050559.
InParanoidi P35626.
KOi K00910.
OMAi QTHLAKK.
OrthoDBi EOG7FV3PP.
PhylomeDBi P35626.
TreeFami TF313940.

Enzyme and pathway databases

BRENDAi 2.7.11.15. 2681.
SignaLinki P35626.

Miscellaneous databases

GeneWikii Beta_adrenergic_receptor_kinase-2.
GenomeRNAii 157.
NextBioi 625.
PROi P35626.
SOURCEi Search...

Gene expression databases

ArrayExpressi P35626.
Bgeei P35626.
CleanExi HS_ADRBK2.
Genevestigatori P35626.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view ]
PRINTSi PR00717. GPCRKINASE.
SMARTi SM00233. PH. 1 hit.
SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, functional expression and mRNA analysis of human beta-adrenergic receptor kinase 2."
    Parruti G., Ambrosini G., Sallese M., de Blasi A.
    Biochem. Biophys. Res. Commun. 190:475-481(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The DNA sequence of human chromosome 22."
    Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M.
    , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
    Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Development and validation of a method for profiling post-translational modification activities using protein microarrays."
    Del Rincon S.V., Rogers J., Widschwendter M., Sun D., Sieburg H.B., Spruck C.
    PLoS ONE 5:E11332-E11332(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION.
  4. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] SER-50; SER-60 AND LYS-104.

Entry informationi

Entry nameiARBK2_HUMAN
AccessioniPrimary (citable) accession number: P35626
Secondary accession number(s): Q9UGW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 17, 2006
Last modified: September 3, 2014
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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