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Reviewed, UniProtKB/Swiss-Prot P35613 (BASI_HUMAN)

Last modified November 3, 2009. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (8) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Basigin
Alternative name(s):
    Leukocyte activation antigen M6
    Collagenase stimulatory factor
    Extracellular matrix metalloproteinase inducer
      Short name=EMMPRIN
    5F7
    Tumor cell-derived collagenase stimulatory factor
      Short name=TCSF
    OK blood group antigen
    CD_antigen=CD147
Gene names
Name: BSG
ORF Names: UNQ6505/PRO21383
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes By similarity.

Subunit structure

Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with ATP1B2, MAG and L1CAM By similarity. Interacts with AJAP1.

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity. Melanosome. Note: Colocalizes with SLC16A1 and SLC16A8 By similarity. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Tissue specificity

Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.

Induction

Enriched on the surface of tumor cells. Up-regulated in gliomas. Its expression levels correlate with malignant potential of the tumor.

Post-translational modification

N-glycosylated. Ref.17 Ref.23 Ref.24

Sequence similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P35613-1)

Also known as: Long; Basigin-2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P35613-2)

The sequence of this isoform differs from the canonical sequence as follows:
     24-139: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Ref.14
Chain22 – 385364Basigin
PRO_0000014518

Regions

Topological domain138 – 323186Extracellular Potential
Transmembrane324 – 34421 Potential
Topological domain345 – 38541Cytoplasmic Potential
Domain138 – 21982Ig-like C2-type
Domain221 – 31595Ig-like V-type
Compositional bias356 – 3594Poly-Asp

Amino acid modifications

Modified residue3621Phosphoserine Ref.18
Modified residue3681Phosphoserine Ref.21
Glycosylation1601N-linked (GlcNAc...) Ref.17 Ref.23 Ref.24
Glycosylation2681N-linked (GlcNAc...) Ref.17 Ref.23 Ref.24
Glycosylation3021N-linked (GlcNAc...) Potential
Disulfide bond157 ↔ 203 By similarity
Disulfide bond242 ↔ 301 By similarity

Natural variations

Alternative sequence24 – 139116Missing in isoform 2.
VSP_011501
Natural variant2081E → K in Ok(A-). Ref.25
VAR_013574
Natural variant2691G → V: dbSNP rs1803203.
VAR_011720

Experimental info

Sequence conflict3281F → L in BAC76828. Ref.2

Secondary structure

.................................... 385
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) (Basigin-2) [UniParc].

Last modified August 31, 2004. Version 2.
Checksum: D74C37455BF26685

FASTA38542,200
        10         20         30         40         50         60 
MAAALFVLLG FALLGTHGAS GAAGFVQAPL SQQRWVGGSV ELHCEAVGSP VPEIQWWFEG 

        70         80         90        100        110        120 
QGPNDTCSQL WDGARLDRVH IHATYHQHAA STISIDTLVE EDTGTYECRA SNDPDRNHLT 

       130        140        150        160        170        180 
RAPRVKWVRA QAVVLVLEPG TVFTTVEDLG SKILLTCSLN DSATEVTGHR WLKGGVVLKE 

       190        200        210        220        230        240 
DALPGQKTEF KVDSDDQWGE YSCVFLPEPM GTANIQLHGP PRVKAVKSSE HINEGETAML 

       250        260        270        280        290        300 
VCKSESVPPV TDWAWYKITD SEDKALMNGS ESRFFVSSSQ GRSELHIENL NMEADPGQYR 

       310        320        330        340        350        360 
CNGTSSKGSD QAIITLRVRS HLAALWPFLG IVAEVLVLVT IIFIYEKRRK PEDVLDDDDA 

       370        380 
GSAPLKSSGQ HQNDKGKNVR QRNSS 

« Hide

Isoform 2.

Checksum: 0C9D6BEB966AC258
Show »

FASTA26929,221

References

« Hide 'large scale' references
[1]"Human leukocyte activation antigen M6, a member of the Ig superfamily, is the species homologue of rat OX-47, mouse basigin, and chicken HT7 molecule."
Kasinrerk W., Fiebiger E., Stefanova I., Baumruker T., Knapp W., Stockinger H.
J. Immunol. 149:847-854(1992) [PubMed: 1634773] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"The basigin group of the immunoglobulin superfamily: complete conservation of a segment in and around transmembrane domains of human and mouse basigin and chicken HT7 antigen."
Miyauchi T., Masuzawa Y., Muramatsu T.
J. Biochem. 110:770-774(1991) [PubMed: 1783610] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
[3]"The human tumor cell-derived collagenase stimulatory factor (renamed EMMPRIN) is a member of the immunoglobulin superfamily."
Biswas C., Zhang Y., Decastro R., Guo H., Nakamura T., Kataoka H., Nabeshima K.
Cancer Res. 55:434-439(1995) [PubMed: 7812975] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]Wakasugi H., Scamps C., Yang G., Vancong N., Bernheim A., Tursz T., Harada N.
Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 2).
[5]Decastro R., Zhang Y., Kataoka H., Coon J., Biswas C.
Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 2).
[6]"Characterization of the gene for human EMMPRIN, a tumor cell surface inducer of matrix metalloproteinases."
Guo H., Majmudar G., Jensen T.C., Biswas C., Toole B.P., Gordon M.K.
Gene 220:99-108(1998) [PubMed: 9767135] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
[7]"Regulation of EMMPRIN/CD147 expression and its function of controlling matrix metalloproteinases production and cell-surface localization in co-culture of human uterine cervical carcinoma SKG-II cells and human uterine cervical fibroblasts."
Sato T., Takita M., Noguchi Y., Hirata M., Sakai T., Ito A.
Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 2).
[8]Kim D., Kanai Y., Choi H., Shin H., Kim J., Teraoka H., Shigeta Y., Chairoungdua A., Babu E., Anzai N., Iribe Y., Endou H.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 2).
[9]"Retina-specific expression of 5A11/Basigin-2, a member of the immunoglobulin gene superfamily."
Ochrietor J.D., Moroz T.P., van Ekeris L., Clamp M.F., Jefferson S.C., deCarvalho A.C., Fadool J.M., Wistow G., Muramatsu T., Linser P.J.
Invest. Ophthalmol. Vis. Sci. 44:4086-4096(2003) [PubMed: 12939332] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
Tissue: Retina.
[10]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[11]SeattleSNPs variation discovery resource
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[12]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed: 15057824] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[13]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Eye.
[14]"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z., Henzel W.J.
Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract]
Cited for: PROTEIN SEQUENCE OF 22-36 (ISOFORM 1).
[15]"Partial sequencing and characterization of the tumor cell-derived collagenase stimulatory factor."
Nabeshima K., Lane W.S., Biswas C.
Arch. Biochem. Biophys. 285:90-96(1991) [PubMed: 1846736] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
[16]"Basigin (CD147): a multifunctional transmembrane protein involved in reproduction, neural function, inflammation and tumor invasion."
Muramatsu T., Miyauchi T.
Histol. Histopathol. 18:981-987(2003) [PubMed: 12792908] [Abstract]
Cited for: REVIEW.
[17]"Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."
Zhang H., Li X.-J., Martin D.B., Aebersold R.
Nat. Biotechnol. 21:660-666(2003) [PubMed: 12754519] [Abstract]
Cited for: GLYCOSYLATION AT ASN-160 AND ASN-268.
[18]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-362, MASS SPECTROMETRY.
Tissue: Epithelium.
[19]"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes."
Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., Abe R., Yamagishi S., Shabanowitz J., Hearing V.J., Wu C., Appella E., Hunt D.F.
J. Proteome Res. 5:3135-3144(2006) [PubMed: 17081065] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[20]"junction protein shrew-1 influences cell invasion and interacts with invasion-promoting protein CD147."
Schreiner A., Ruonala M., Jakob V., Suthaus J., Boles E., Wouters F., Starzinski-Powitz A.
Mol. Biol. Cell 18:1272-1281(2007) [PubMed: 17267690] [Abstract]
Cited for: INTERACTION WITH AJAP1.
[21]"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry."
Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A.
Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-368, MASS SPECTROMETRY.
[22]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[23]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-160 AND ASN-268, MASS SPECTROMETRY.
Tissue: Liver.
[24]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-160 AND ASN-268, MASS SPECTROMETRY.
[25]"The Ok(a) blood group antigen is a marker for the M6 leukocyte activation antigen, the human homolog of OX-47 antigen, basigin and neurothelin, an immunoglobulin superfamily molecule that is widely expressed in human cells and tissues."
Spring F.A., Holmes C.H., Simpson K.L., Mawby W.J., Mattes M.J., Okubo Y., Parsons S.F.
Eur. J. Immunol. 27:891-897(1997) [PubMed: 9130641] [Abstract]
Cited for: VARIANT LYS-208.
+Additional computationally mapped references.

Web resources

dbRBC/BGMUT

Blood group antigen gene mutation database

SeattleSNPs

Cross-references

Sequence databases

X64364 mRNA. Translation: CAA45716.1.
D45131 mRNA. Translation: BAA08109.1.
L10240 mRNA. Translation: AAA68936.1.
M87879 mRNA. Translation: AAA91084.1.
L20471 mRNA. Translation: AAB41120.1.
AF042854 expand/collapse EMBL AC list , AF042848, AF042849, AF042850, AF042851, AF042852, AF042853 Genomic DNA. Translation: AAD10704.1.
AB072923 mRNA. Translation: BAB88938.1.
AB085790 mRNA. Translation: BAC76828.1.
AF548371 mRNA. Translation: AAN40694.1.
AY358113 mRNA. Translation: AAQ88480.1.
AY942196 Genomic DNA. Translation: AAX20110.1.
AC005559 Genomic DNA. Translation: AAC33279.1.
BC009040 mRNA. Translation: AAH09040.1.
IPIIPI00019906.
IPI00218019.
PIRA46506.
RefSeqNP_001719.2.
NP_940991.1.
NP_940993.1.
UniGeneHs.591382

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
3B5HX-ray2.80A/B/C/D140-321[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP35613. 5 interactions.
STRINGP35613.

Protein family/group databases

TCDB8.A.23.1.1. basigin family.

PTM databases

PhosphoSiteP35613.

Proteomic databases

PRIDEP35613.

Genome annotation databases

EnsemblENST00000333511; ENSP00000333769; ENSG00000172270; Homo sapiens. [Genome view]
ENST00000346916; ENSP00000344707; ENSG00000172270; Homo sapiens. [Genome view]
ENST00000353555; ENSP00000343809; ENSG00000172270; Homo sapiens. [Genome view]
ENST00000396012; ENSP00000379333; ENSG00000172270; Homo sapiens. [Genome view]
GeneID682.
KEGGhsa:682.
UCSCuc002loz.1. human.

Organism-specific databases

CTD682.
GeneCardsGC19P000522.
H-InvDBHIX0014556.
HGNCHGNC:1116. BSG.
HPACAB002427.
MIM109480. gene.
111380. phenotype.
PharmGKBPA25433.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP35613.
HOVERGENP35613.
OMADRNHLTR.

Enzyme and pathway databases

Pathway_Interaction_DBsyndecan_1_pathway. Syndecan-1-mediated signaling events.
ReactomeREACT_1046. Pyruvate metabolism and TCA cycle.
REACT_13552. Integrin cell surface interactions.
REACT_474. Metabolism of carbohydrates.
REACT_604. Hemostasis.
REACT_6900. Signaling in Immune system.

Gene expression databases

ArrayExpressP35613.
BgeeP35613.
CleanExHS_BSG.
GenevestigatorP35613.
GermOnlineENSG00000172270. Homo sapiens.

Family and domain databases

InterProIPR013151. Ig.
IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
Gene3DG3DSA:2.60.40.10. Ig-like_fold. 2 hits.
PfamPF00047. ig. 2 hits.
[Graphical view]
SMARTSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 3 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio2802.
PMAP-CutDBP35613.
SOURCESearch...

Entry information

Entry nameBASI_HUMAN
AccessionPrimary (citable) accession number: P35613
Secondary accession number(s): Q7Z796, Q8IZL7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: August 31, 2004
Last modified: November 3, 2009
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Blood group antigen proteins

Nomenclature of blood group antigens and list of entries

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents