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Protein

Coatomer subunit beta'

Gene

COPB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).By similarity
This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner (By similarity). Interacts with JAGN1.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
SignaLinkiP35606.

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit beta'
Alternative name(s):
Beta'-coat protein
Short name:
Beta'-COP
p102
Gene namesi
Name:COPB2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:2232. COPB2.

Subcellular locationi

GO - Cellular componenti

  • COPI vesicle coat Source: UniProtKB
  • cytosol Source: Reactome
  • endoplasmic reticulum membrane Source: Reactome
  • Golgi membrane Source: Reactome
  • transport vesicle Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi9276.
OpenTargetsiENSG00000184432.
PharmGKBiPA26748.

Polymorphism and mutation databases

BioMutaiCOPB2.
DMDMi544076.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000509122 – 906Coatomer subunit beta'Add BLAST905

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei627N6-acetyllysineCombined sources1
Modified residuei859PhosphoserineCombined sources1
Modified residuei861PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP35606.
MaxQBiP35606.
PaxDbiP35606.
PeptideAtlasiP35606.
PRIDEiP35606.

PTM databases

iPTMnetiP35606.
PhosphoSitePlusiP35606.
SwissPalmiP35606.

Expressioni

Gene expression databases

BgeeiENSG00000184432.
CleanExiHS_COPB2.
ExpressionAtlasiP35606. baseline and differential.
GenevisibleiP35606. HS.

Organism-specific databases

HPAiHPA036867.
HPA058180.

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Probably interacts with PEX11A. Interacts with SCYL1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
NFKB1P198383EBI-1056534,EBI-300010

Protein-protein interaction databases

BioGridi114693. 49 interactors.
DIPiDIP-27603N.
IntActiP35606. 29 interactors.
MINTiMINT-5000992.
STRINGi9606.ENSP00000329419.

Structurei

3D structure databases

ProteinModelPortaliP35606.
SMRiP35606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati13 – 52WD 1Add BLAST40
Repeati55 – 94WD 2Add BLAST40
Repeati97 – 136WD 3Add BLAST40
Repeati140 – 180WD 4Add BLAST41
Repeati183 – 224WD 5Add BLAST42
Repeati227 – 266WD 6Add BLAST40

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili866 – 891Sequence analysisAdd BLAST26

Sequence similaritiesi

Belongs to the WD repeat COPB2 family.Curated
Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0276. Eukaryota.
ENOG410XNNY. LUCA.
GeneTreeiENSGT00780000122009.
HOGENOMiHOG000163444.
HOVERGENiHBG051076.
KOiK17302.
OMAiSQENNEG.
OrthoDBiEOG091G0284.
PhylomeDBiP35606.
TreeFamiTF300688.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR006692. Coatomer_WD-assoc_reg.
IPR016453. COPB2.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF04053. Coatomer_WDAD. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PIRSFiPIRSF005567. Coatomer_beta'_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
PROSITEiPS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P35606-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLRLDIKRK LTARSDRVKS VDLHPTEPWM LASLYNGSVC VWNHETQTLV
60 70 80 90 100
KTFEVCDLPV RAAKFVARKN WVVTGADDMQ IRVFNYNTLE RVHMFEAHSD
110 120 130 140 150
YIRCIAVHPT QPFILTSSDD MLIKLWDWDK KWSCSQVFEG HTHYVMQIVI
160 170 180 190 200
NPKDNNQFAS ASLDRTIKVW QLGSSSPNFT LEGHEKGVNC IDYYSGGDKP
210 220 230 240 250
YLISGADDRL VKIWDYQNKT CVQTLEGHAQ NVSCASFHPE LPIIITGSED
260 270 280 290 300
GTVRIWHSST YRLESTLNYG MERVWCVASL RGSNNVALGY DEGSIIVKLG
310 320 330 340 350
REEPAMSMDA NGKIIWAKHS EVQQANLKAM GDAEIKDGER LPLAVKDMGS
360 370 380 390 400
CEIYPQTIQH NPNGRFVVVC GDGEYIIYTA MALRNKSFGS AQEFAWAHDS
410 420 430 440 450
SEYAIRESNS IVKIFKNFKE KKSFKPDFGA ESIYGGFLLG VRSVNGLAFY
460 470 480 490 500
DWDNTELIRR IEIQPKHIFW SDSGELVCIA TEESFFILKY LSEKVLAAQE
510 520 530 540 550
THEGVTEDGI EDAFEVLGEI QEIVKTGLWV GDCFIYTSSV NRLNYYVGGE
560 570 580 590 600
IVTIAHLDRT MYLLGYIPKD NRLYLGDKEL NIISYSLLVS VLEYQTAVMR
610 620 630 640 650
RDFSMADKVL PTIPKEQRTR VAHFLEKQGF KQQALTVSTD PEHRFELALQ
660 670 680 690 700
LGELKIAYQL AVEAESEQKW KQLAELAISK CQFGLAQECL HHAQDYGGLL
710 720 730 740 750
LLATASGNAN MVNKLAEGAE RDGKNNVAFM SYFLQGKVDA CLELLIRTGR
760 770 780 790 800
LPEAAFLART YLPSQVSRVV KLWRENLSKV NQKAAESLAD PTEYENLFPG
810 820 830 840 850
LKEAFVVEEW VKETHADLWP AKQYPLVTPN EERNVMEEGK DFQPSRSTAQ
860 870 880 890 900
QELDGKPASP TPVIVASHTA NKEEKSLLEL EVDLDNLELE DIDTTDINLD

EDILDD
Length:906
Mass (Da):102,487
Last modified:January 23, 2007 - v2
Checksum:i824BB63BEFAA53F6
GO
Isoform 2 (identifier: P35606-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Note: No experimental confirmation available.
Show »
Length:877
Mass (Da):99,046
Checksum:i9E91476A4379BB1C
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0561651 – 29Missing in isoform 2. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70476 mRNA. Translation: CAA49900.1.
AK302943 mRNA. Translation: BAG64100.1.
AC024933 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79040.1.
BC000326 mRNA. Translation: AAH00326.1.
CCDSiCCDS3108.1. [P35606-1]
PIRiS35342.
RefSeqiNP_004757.1. NM_004766.2. [P35606-1]
XP_016863001.1. XM_017007512.1. [P35606-2]
UniGeneiHs.681616.

Genome annotation databases

EnsembliENST00000333188; ENSP00000329419; ENSG00000184432. [P35606-1]
ENST00000507777; ENSP00000422295; ENSG00000184432. [P35606-2]
GeneIDi9276.
KEGGihsa:9276.
UCSCiuc003etf.5. human. [P35606-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70476 mRNA. Translation: CAA49900.1.
AK302943 mRNA. Translation: BAG64100.1.
AC024933 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79040.1.
BC000326 mRNA. Translation: AAH00326.1.
CCDSiCCDS3108.1. [P35606-1]
PIRiS35342.
RefSeqiNP_004757.1. NM_004766.2. [P35606-1]
XP_016863001.1. XM_017007512.1. [P35606-2]
UniGeneiHs.681616.

3D structure databases

ProteinModelPortaliP35606.
SMRiP35606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114693. 49 interactors.
DIPiDIP-27603N.
IntActiP35606. 29 interactors.
MINTiMINT-5000992.
STRINGi9606.ENSP00000329419.

PTM databases

iPTMnetiP35606.
PhosphoSitePlusiP35606.
SwissPalmiP35606.

Polymorphism and mutation databases

BioMutaiCOPB2.
DMDMi544076.

Proteomic databases

EPDiP35606.
MaxQBiP35606.
PaxDbiP35606.
PeptideAtlasiP35606.
PRIDEiP35606.

Protocols and materials databases

DNASUi9276.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000333188; ENSP00000329419; ENSG00000184432. [P35606-1]
ENST00000507777; ENSP00000422295; ENSG00000184432. [P35606-2]
GeneIDi9276.
KEGGihsa:9276.
UCSCiuc003etf.5. human. [P35606-1]

Organism-specific databases

CTDi9276.
DisGeNETi9276.
GeneCardsiCOPB2.
HGNCiHGNC:2232. COPB2.
HPAiHPA036867.
HPA058180.
MIMi606990. gene.
neXtProtiNX_P35606.
OpenTargetsiENSG00000184432.
PharmGKBiPA26748.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0276. Eukaryota.
ENOG410XNNY. LUCA.
GeneTreeiENSGT00780000122009.
HOGENOMiHOG000163444.
HOVERGENiHBG051076.
KOiK17302.
OMAiSQENNEG.
OrthoDBiEOG091G0284.
PhylomeDBiP35606.
TreeFamiTF300688.

Enzyme and pathway databases

ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
SignaLinkiP35606.

Miscellaneous databases

ChiTaRSiCOPB2. human.
GeneWikiiCOPB2.
GenomeRNAii9276.
PROiP35606.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000184432.
CleanExiHS_COPB2.
ExpressionAtlasiP35606. baseline and differential.
GenevisibleiP35606. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR006692. Coatomer_WD-assoc_reg.
IPR016453. COPB2.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF04053. Coatomer_WDAD. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PIRSFiPIRSF005567. Coatomer_beta'_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
PROSITEiPS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOPB2_HUMAN
AccessioniPrimary (citable) accession number: P35606
Secondary accession number(s): B4DZI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 170 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.