Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Coatomer subunit beta'

Gene

COPB2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).By similarity
This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: GO_Central
  • intracellular protein transport Source: GO_Central
  • intra-Golgi vesicle-mediated transport Source: UniProtKB
  • retrograde vesicle-mediated transport, Golgi to ER Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit beta'
Alternative name(s):
Beta'-coat protein
Short name:
Beta'-COP
p102
Gene namesi
Name:COPB2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  • COPI vesicle coat Source: UniProtKB
  • cytosol Source: UniProtKB-SubCell
  • Golgi apparatus Source: AgBase
  • Golgi membrane Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 906905Coatomer subunit beta'PRO_0000050911Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei627 – 6271N6-acetyllysineBy similarity
Modified residuei859 – 8591PhosphoserineBy similarity
Modified residuei861 – 8611PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP35605.
PeptideAtlasiP35605.
PRIDEiP35605.

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Probably interacts with PEX11A. Interacts with SCYL1. Interacts with JAGN1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
COPAQ279542EBI-620411,EBI-620400

GO - Molecular functioni

Protein-protein interaction databases

IntActiP35605. 1 interaction.
STRINGi9913.ENSBTAP00000019767.

Structurei

3D structure databases

ProteinModelPortaliP35605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati13 – 5240WD 1Add
BLAST
Repeati55 – 9440WD 2Add
BLAST
Repeati97 – 13640WD 3Add
BLAST
Repeati140 – 18041WD 4Add
BLAST
Repeati183 – 22442WD 5Add
BLAST
Repeati227 – 26640WD 6Add
BLAST
Repeati350 – 38839WD 7Add
BLAST
Repeati390 – 42536WD 8Add
BLAST
Repeati746 – 78338WD 9Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili866 – 89126Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the WD repeat COPB2 family.Curated
Contains 9 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0276. Eukaryota.
ENOG410XNNY. LUCA.
HOGENOMiHOG000163444.
HOVERGENiHBG051076.
InParanoidiP35605.
KOiK17302.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR006692. Coatomer_WD-assoc_reg.
IPR016453. COPB2.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF04053. Coatomer_WDAD. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PIRSFiPIRSF005567. Coatomer_beta'_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
PROSITEiPS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLRLDIKRK LTARSDRVKS VDLHPTEPWM LASLYNGSVC VWNHETQTLV
60 70 80 90 100
KTFEVCDLPV RAAKFVARKN WVVTGADDMQ IRVFNYNTLE RVHMFEAHSD
110 120 130 140 150
YIRCIAVHPT QPFILTSSDD MLIKLWDWDK KWSCSQVFEG HTHYVMQIVI
160 170 180 190 200
NPKDNNQFAS ASLDRTIKVW QLGSSSPNFT LEGHEKGVNC IDYYSGGDKP
210 220 230 240 250
YLISGADDRL VKIWDYQNKT CVQTLEGHAQ NVSCASFHPE LPIIITGSED
260 270 280 290 300
GTVRIWHSST YRLESTLNYG MERVWCVASL RGSNNVALGY DEGSIIVKLG
310 320 330 340 350
REEPAMSMDA NGKIIWAKHS EVQQANLKAM GDAEIKDGER LPLAVKDMGS
360 370 380 390 400
CEIYPQTIQH NPNGRFVVVC GDGEYIIYTA MALRNKSFGS AQEFAWAHDS
410 420 430 440 450
SEYAIRESNS VVKIFKNFKE KKSFKPDFGA ESIYGGFLLG VRSVNGLAFY
460 470 480 490 500
DWENTELIRR IEIQPKHIFW SDSGELVCIA TEESFFILKY LSEKVLAAQE
510 520 530 540 550
THEGVTEDGI EDGFEVLGEI QEIVKTGLWV GDCFIYTSSV NRLNYYVGGE
560 570 580 590 600
IVTIAHLDRT MYLLGYIPKD NRLYLGDKEL NIVSYSLLVS VLEYQTAVMR
610 620 630 640 650
RDFSMADKVL PTIPKEQRTR VAHFLEKQGF KQQALTVSTD PEHRFELALQ
660 670 680 690 700
LGELKIAYQL AVEAESEQKW KQLAELAISK CQFGLAQECL HHAQDYGGLL
710 720 730 740 750
LLATASGNAS MVNKLAEGAE RDGKNNVAFM SYFLQGKLDA CLELLIRTGR
760 770 780 790 800
LPEAAFLART YLPSQVSRVV KLWRENLSKV NQKAAESLAD PTEYENLFPG
810 820 830 840 850
LKEAFVVEEW VKETHADLWP AKQYPLVTPN EERNVMEEAK GFQPSRSAAQ
860 870 880 890 900
QELDGKPASP TPVIVTSQTA NKEEKSLLEL EVDLDNLEIE DIDTTDINLD

EDILDD
Length:906
Mass (Da):102,393
Last modified:April 29, 2008 - v3
Checksum:iE34526000E424E88
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti682 – 6821Q → P in CAA51285 (PubMed:8334999).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72756 mRNA. Translation: CAA51285.1.
BC134537 mRNA. Translation: AAI34538.1.
PIRiS35312.
RefSeqiNP_776706.2. NM_174281.3.
UniGeneiBt.4801.

Genome annotation databases

GeneIDi281706.
KEGGibta:281706.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72756 mRNA. Translation: CAA51285.1.
BC134537 mRNA. Translation: AAI34538.1.
PIRiS35312.
RefSeqiNP_776706.2. NM_174281.3.
UniGeneiBt.4801.

3D structure databases

ProteinModelPortaliP35605.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP35605. 1 interaction.
STRINGi9913.ENSBTAP00000019767.

Proteomic databases

PaxDbiP35605.
PeptideAtlasiP35605.
PRIDEiP35605.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281706.
KEGGibta:281706.

Organism-specific databases

CTDi9276.

Phylogenomic databases

eggNOGiKOG0276. Eukaryota.
ENOG410XNNY. LUCA.
HOGENOMiHOG000163444.
HOVERGENiHBG051076.
InParanoidiP35605.
KOiK17302.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR006692. Coatomer_WD-assoc_reg.
IPR016453. COPB2.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF04053. Coatomer_WDAD. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PIRSFiPIRSF005567. Coatomer_beta'_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
PROSITEiPS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOPB2_BOVIN
AccessioniPrimary (citable) accession number: P35605
Secondary accession number(s): A4IFE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: April 29, 2008
Last modified: July 6, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.