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Protein

AP-2 complex subunit mu

Gene

dpy-23

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the adaptor complexes which link clathrin to receptors in coated vesicles (By similarity). Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration (By similarity). AP50 is a subunit of the plasma membrane adaptor (By similarity). Essential wnt/egl-20 signaling protein that functions in wnt/egl-20-producing cells (PubMed:18160346, PubMed:18160347). Required for the AP-2 complex-mediated endocytosis of membrane proteins including wntless homolog mig-14 in egl-20-producing cells (PubMed:18160346, PubMed:18160347). During development, regulates the migration of HSN neurons and the left and right Q neuroblasts (QL and QR, respectively) and their descendants, possibly through hox gene and wnt/egl-20 gene target mab-5, and plays a role in establishing ALM and PLM neuronal cell polarity (PubMed:18160346). Required for the asymmetric divisions of V5 cells (PubMed:18160346).By similarity2 Publications

GO - Biological processi

  • body morphogenesis Source: WormBase
  • intracellular protein transport Source: InterPro
  • positive regulation of necrotic cell death Source: WormBase
  • positive regulation of Wnt protein secretion Source: WormBase
  • protein localization to nonmotile primary cilium Source: WormBase
  • receptor-mediated endocytosis Source: WormBase
Complete GO annotation...

Keywords - Biological processi

Endocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-CEL-437239. Recycling pathway of L1.
R-CEL-5099900. WNT5A-dependent internalization of FZD4.
SignaLinkiP35603.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-2 complex subunit mu
Alternative name(s):
Clathrin assembly protein complex 2 mu medium chain
Clathrin coat assembly protein AP50
Clathrin coat-associated protein AP50
Mu2-adaptin
Plasma membrane adaptor AP-2 50 kDa protein
Protein dumpy-23
Gene namesi
Name:dpy-23
Synonyms:ap50
ORF Names:R160.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiR160.1a; CE33813; WBGene00001082; dpy-23.
R160.1b; CE33814; WBGene00001082; dpy-23.

Subcellular locationi

GO - Cellular componenti

  • clathrin adaptor complex Source: InterPro
  • coated pit Source: UniProtKB-SubCell
  • periciliary membrane compartment Source: WormBase
  • synapse Source: WormBase
  • synaptic vesicle Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Coated pit, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants display a squat body statue referred to as a dumpy phenotype and have defective HSN neuronal cell migration (PubMed:18160346). RNAi-mediated knockdown results in impaired function of the AP-2 complex and accumulation of the wntless homolog mig-14 at the cell membrane (PubMed:18160347).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 441441AP-2 complex subunit muPRO_0000193791Add
BLAST

Proteomic databases

EPDiP35603.
PaxDbiP35603.
PRIDEiP35603.

Expressioni

Tissue specificityi

Brain, heart, lung, liver, testis and spleen.

Interactioni

Subunit structurei

Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit and beta-type subunits), a medium adaptin (mu-type subunit AP50) and a small adaptin (sigma-type subunit AP17).

Protein-protein interaction databases

BioGridi45648. 2 interactions.
STRINGi6239.R160.1a.

Structurei

3D structure databases

ProteinModelPortaliP35603.
SMRiP35603. Positions 1-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini174 – 440267MHDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0937. Eukaryota.
ENOG410XPFS. LUCA.
GeneTreeiENSGT00530000062779.
HOGENOMiHOG000173246.
InParanoidiP35603.
KOiK11826.
OMAiVWKIPRI.
OrthoDBiEOG72RMXV.
PhylomeDBiP35603.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P35603-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIGGLFVYNH KGEVLISRIY RDDVTRNAVD AFRVNVIHAR QQVRSPVTNM
60 70 80 90 100
ARTSFFHVKR GNVWICAVTR QNVNAAMVFE FLKRFADTMQ SYFGKLNEEN
110 120 130 140 150
VKNNFVLIYE LLDEILDFGY PQNTDPGVLK TFITQQGVRT ADAPVPVTKE
160 170 180 190 200
EQSQITSQVT GQIGWRREGI KYRRNELFLD VIEYVNLLMN QQGQVLSAHV
210 220 230 240 250
AGKVAMKSYL SGMPECKFGI NDKITIEGKS KPGSDDPNKA SRAAVAIDDC
260 270 280 290 300
QFHQCVKLTK FETEHAISFI PPDGEYELMR YRTTKDIQLP FRVIPLVREV
310 320 330 340 350
SRNKMEVKVV VKSNFKPSLL AQKLEVRIPT PPNTSGVQLI CMKGKAKYKA
360 370 380 390 400
GENAIVWKIK RMAGMKESQI SAEIDLLSTG NVEKKKWNRP PVSMNFEVPF
410 420 430 440
APSGLKVRYL KVFEPKLNYS DHDVIKWVRY IGRSGLYETR C
Length:441
Mass (Da):50,300
Last modified:August 15, 2003 - v2
Checksum:iD8F8B7760CAB164F
GO
Isoform b (identifier: P35603-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     142-147: Missing.

Note: No experimental confirmation available.
Show »
Length:435
Mass (Da):49,722
Checksum:iDDF71D98EF0534D0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti80 – 801E → A in AAA27981 (PubMed:8288128).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei142 – 1476Missing in isoform b. CuratedVSP_008037

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26290 mRNA. Translation: AAA27981.1.
FO081006 Genomic DNA. Translation: CCD68418.1.
FO081006 Genomic DNA. Translation: CCD68419.1.
PIRiB49837.
RefSeqiNP_001024865.1. NM_001029694.3. [P35603-2]
NP_741770.1. NM_171670.4. [P35603-1]
UniGeneiCel.6847.

Genome annotation databases

EnsemblMetazoaiR160.1a; R160.1a; WBGene00001082. [P35603-1]
GeneIDi180713.
KEGGicel:CELE_R160.1.
UCSCiR160.1b. c. elegans. [P35603-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26290 mRNA. Translation: AAA27981.1.
FO081006 Genomic DNA. Translation: CCD68418.1.
FO081006 Genomic DNA. Translation: CCD68419.1.
PIRiB49837.
RefSeqiNP_001024865.1. NM_001029694.3. [P35603-2]
NP_741770.1. NM_171670.4. [P35603-1]
UniGeneiCel.6847.

3D structure databases

ProteinModelPortaliP35603.
SMRiP35603. Positions 1-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi45648. 2 interactions.
STRINGi6239.R160.1a.

Proteomic databases

EPDiP35603.
PaxDbiP35603.
PRIDEiP35603.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiR160.1a; R160.1a; WBGene00001082. [P35603-1]
GeneIDi180713.
KEGGicel:CELE_R160.1.
UCSCiR160.1b. c. elegans. [P35603-1]

Organism-specific databases

CTDi180713.
WormBaseiR160.1a; CE33813; WBGene00001082; dpy-23.
R160.1b; CE33814; WBGene00001082; dpy-23.

Phylogenomic databases

eggNOGiKOG0937. Eukaryota.
ENOG410XPFS. LUCA.
GeneTreeiENSGT00530000062779.
HOGENOMiHOG000173246.
InParanoidiP35603.
KOiK11826.
OMAiVWKIPRI.
OrthoDBiEOG72RMXV.
PhylomeDBiP35603.

Enzyme and pathway databases

ReactomeiR-CEL-437239. Recycling pathway of L1.
R-CEL-5099900. WNT5A-dependent internalization of FZD4.
SignaLinkiP35603.

Miscellaneous databases

PROiP35603.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "unc-101, a gene required for many aspects of Caenorhabditis elegans development and behavior, encodes a clathrin-associated protein."
    Lee J., Jongeward G.D., Sternberg P.W.
    Genes Dev. 8:60-73(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "C. elegans AP-2 and retromer control Wnt signaling by regulating mig-14/Wntless."
    Pan C.L., Baum P.D., Gu M., Jorgensen E.M., Clark S.G., Garriga G.
    Dev. Cell 14:132-139(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  4. "Wnt signaling requires retromer-dependent recycling of MIG-14/Wntless in Wnt-producing cells."
    Yang P.T., Lorenowicz M.J., Silhankova M., Coudreuse D.Y., Betist M.C., Korswagen H.C.
    Dev. Cell 14:140-147(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiAP2M_CAEEL
AccessioniPrimary (citable) accession number: P35603
Secondary accession number(s): Q8MXF2, Q9TZC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: August 15, 2003
Last modified: June 8, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.