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Protein

AP-1 complex subunit mu-1-I

Gene

unc-101

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Ap47 is a subunit of the plasma membrane adaptor.1 Publication
Required for many aspects of development and behavior, including negative regulation of vulval differentiation.1 Publication

GO - Biological processi

  • apical protein localization Source: WormBase
  • cilium assembly Source: WormBase
  • embryo development ending in birth or egg hatching Source: WormBase
  • intracellular protein transport Source: UniProtKB
  • nematode larval development Source: WormBase
  • receptor localization to nonmotile primary cilium Source: WormBase
  • vesicle-mediated transport Source: InterPro
  • vulval development Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

SignaLinkiP35602.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit mu-1-I
Alternative name(s):
Clathrin assembly protein complex 1 mu-1-I medium chain
Clathrin coat assembly protein AP47
Clathrin coat-associated protein AP47
Golgi adaptor AP-1 47 kDa protein
HA1 47 kDa subunit
Mu1-I-adaptin
Uncoordinated protein 101
Gene namesi
Name:unc-101
ORF Names:K11D2.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiK11D2.3a; CE28050; WBGene00006829; unc-101.
K11D2.3b; CE40095; WBGene00006829; unc-101.

Subcellular locationi

GO - Cellular componenti

  • AP-1 adaptor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 422422AP-1 complex subunit mu-1-IPRO_0000193789Add
BLAST

Proteomic databases

EPDiP35602.
PaxDbiP35602.
PRIDEiP35602.

Expressioni

Gene expression databases

BgeeiWBGene00006829.
ExpressionAtlasiP35602. baseline.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma- and beta'-type subunits), a medium adaptin (mu-type subunit AP47) and a small adaptin (sigma-type subunit AP19).

Protein-protein interaction databases

BioGridi38518. 7 interactions.
IntActiP35602. 9 interactions.
MINTiMINT-114772.
STRINGi6239.K11D2.3a.1.

Structurei

3D structure databases

ProteinModelPortaliP35602.
SMRiP35602. Positions 2-422.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini167 – 420254MHDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0937. Eukaryota.
ENOG410XPFS. LUCA.
GeneTreeiENSGT00530000062779.
HOGENOMiHOG000173247.
InParanoidiP35602.
KOiK12393.
OMAiITQNGEY.
OrthoDBiEOG091G06XI.
PhylomeDBiP35602.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P35602-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATSAMFILD LKGKTIISRN YRGDIDMTAI DKFIHLLMEK EEEGSAAPVL
60 70 80 90 100
TYQDTNFVFI KHTNIYLVSA CRSNVNVTMI LSFLYKCVEV FSEYFKDVEE
110 120 130 140 150
ESVRDNFVVI YELLDEMMDF GFPQTTESRI LQEYITQEGQ KLISAPRPPM
160 170 180 190 200
AVTNAVSWRS EGIKYRKNEV FLDVIESVNM LASANGTVLQ SEIVGSVKMR
210 220 230 240 250
VYLTGMPELR LGLNDKVLFE GSGRGKSKSV ELEDVKFHQC VRLSRFDTDR
260 270 280 290 300
TISFIPPDGA FELMSYRLTT VVKPLIWIET SIERHSHSRV SFIIKAKSQF
310 320 330 340 350
KRRSTANNVE IIIPVPSDAD SPKFKTSIGS VKYTPEQSAF VWTIKNFPGG
360 370 380 390 400
KEYLLTAHLS LPSVMSEESE GRPPIKVKFE IPYFTTSGIQ VRYLKIIEKS
410 420
GYQALPWVRY ITQNGEYEMR MK
Length:422
Mass (Da):48,229
Last modified:June 20, 2002 - v2
Checksum:i947BF5D1EF496C8B
GO
Isoform b (identifier: P35602-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-364: Missing.

Note: No experimental confirmation available.
Show »
Length:58
Mass (Da):6,918
Checksum:i0873531DD18A910C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti400 – 4001S → R in AAA72418 (PubMed:8288128).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 364364Missing in isoform b. CuratedVSP_020097Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26291 mRNA. Translation: AAA72418.1.
Z83115 Genomic DNA. Translation: CAB05557.3.
Z83115 Genomic DNA. Translation: CAJ85757.1.
PIRiA49837.
T23603.
RefSeqiNP_001040675.1. NM_001047210.2. [P35602-1]
NP_001040676.1. NM_001047211.1. [P35602-2]
UniGeneiCel.19746.

Genome annotation databases

EnsemblMetazoaiK11D2.3a.1; K11D2.3a.1; WBGene00006829. [P35602-1]
K11D2.3a.2; K11D2.3a.2; WBGene00006829. [P35602-1]
GeneIDi173121.
KEGGicel:CELE_K11D2.3.
UCSCiK11D2.3a.1. c. elegans. [P35602-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26291 mRNA. Translation: AAA72418.1.
Z83115 Genomic DNA. Translation: CAB05557.3.
Z83115 Genomic DNA. Translation: CAJ85757.1.
PIRiA49837.
T23603.
RefSeqiNP_001040675.1. NM_001047210.2. [P35602-1]
NP_001040676.1. NM_001047211.1. [P35602-2]
UniGeneiCel.19746.

3D structure databases

ProteinModelPortaliP35602.
SMRiP35602. Positions 2-422.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi38518. 7 interactions.
IntActiP35602. 9 interactions.
MINTiMINT-114772.
STRINGi6239.K11D2.3a.1.

Proteomic databases

EPDiP35602.
PaxDbiP35602.
PRIDEiP35602.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiK11D2.3a.1; K11D2.3a.1; WBGene00006829. [P35602-1]
K11D2.3a.2; K11D2.3a.2; WBGene00006829. [P35602-1]
GeneIDi173121.
KEGGicel:CELE_K11D2.3.
UCSCiK11D2.3a.1. c. elegans. [P35602-1]

Organism-specific databases

CTDi173121.
WormBaseiK11D2.3a; CE28050; WBGene00006829; unc-101.
K11D2.3b; CE40095; WBGene00006829; unc-101.

Phylogenomic databases

eggNOGiKOG0937. Eukaryota.
ENOG410XPFS. LUCA.
GeneTreeiENSGT00530000062779.
HOGENOMiHOG000173247.
InParanoidiP35602.
KOiK12393.
OMAiITQNGEY.
OrthoDBiEOG091G06XI.
PhylomeDBiP35602.

Enzyme and pathway databases

SignaLinkiP35602.

Miscellaneous databases

PROiP35602.

Gene expression databases

BgeeiWBGene00006829.
ExpressionAtlasiP35602. baseline.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAP1M_CAEEL
AccessioniPrimary (citable) accession number: P35602
Secondary accession number(s): O02282, Q1ZXR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 20, 2002
Last modified: September 7, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.