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P35601

- RFC1_MOUSE

UniProt

P35601 - RFC1_MOUSE

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Protein

Replication factor C subunit 1

Gene

Rfc1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi635 – 6428ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: RefGenome
  3. DNA clamp loader activity Source: InterPro

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

DNA replication, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C subunit 1
Alternative name(s):
A1-P145
Activator 1 140 kDa subunit
Short name:
A1 140 kDa subunit
Activator 1 large subunit
Activator 1 subunit 1
Differentiation-specific element-binding protein
ISRE-binding protein
Replication factor C 140 kDa subunit
Short name:
RF-C 140 kDa subunit
Short name:
RFC140
Replication factor C large subunit
Gene namesi
Name:Rfc1
Synonyms:Ibf-1, Recc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:97891. Rfc1.

Subcellular locationi

GO - Cellular componenti

  1. DNA replication factor C complex Source: InterPro
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11311131Replication factor C subunit 1PRO_0000121773Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei66 – 661PhosphotyrosineBy similarity
Modified residuei68 – 681Phosphoserine1 Publication
Modified residuei70 – 701Phosphoserine1 Publication
Modified residuei72 – 721PhosphoserineBy similarity
Modified residuei107 – 1071Phosphoserine1 Publication
Modified residuei109 – 1091Phosphothreonine1 Publication
Modified residuei155 – 1551PhosphoserineBy similarity
Modified residuei160 – 1601PhosphothreonineBy similarity
Modified residuei162 – 1621PhosphothreonineBy similarity
Modified residuei163 – 1631PhosphoserineBy similarity
Modified residuei172 – 1721PhosphoserineBy similarity
Modified residuei189 – 1891PhosphoserineBy similarity
Modified residuei244 – 2441Phosphoserine1 Publication
Modified residuei281 – 2811PhosphoserineBy similarity
Modified residuei309 – 3091PhosphoserineBy similarity
Modified residuei365 – 3651PhosphoserineBy similarity
Modified residuei1090 – 10901PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35601.
PaxDbiP35601.
PRIDEiP35601.

PTM databases

PhosphoSiteiP35601.

Expressioni

Gene expression databases

CleanExiMM_RFC1.
GenevestigatoriP35601.

Interactioni

Subunit structurei

Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA (By similarity).By similarity

Protein-protein interaction databases

BioGridi202846. 6 interactions.
MINTiMINT-4084942.

Structurei

3D structure databases

ProteinModelPortaliP35601.
SMRiP35601. Positions 372-479, 570-829.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini399 – 48991BRCTPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni354 – 528175Interferon-stimulated-response-element binding regionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1104 – 11085Nuclear localization signalSequence Analysis

Sequence similaritiesi

Belongs to the activator 1 large subunit family.Curated
Contains 1 BRCT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5275.
HOVERGENiHBG004167.
InParanoidiP35601.
KOiK10754.
PhylomeDBiP35601.

Family and domain databases

Gene3Di3.40.50.10190. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001357. BRCT_dom.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR012178. DNA_replication_fac_C_lsu.
IPR013725. DNA_replication_fac_RFC1_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF00533. BRCT. 1 hit.
PF08519. RFC1. 1 hit.
[Graphical view]
PIRSFiPIRSF036578. RFC1. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52113. SSF52113. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P35601-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDIRKFFGVI SSGKKPVNET VKNEKTKASE GTVKGKKGVK EAKVNNSGKE
60 70 80 90 100
DASKPKQHSK KKRIIYDSDS ESEETVQVKN AKKKSEKLSL SYKPGKVSQK
110 120 130 140 150
DPVTYVSETD EDDDFVCKKA ASKSKENGVS TNSYLGTSNV KKNEENVKTK
160 170 180 190 200
NKPLSPIKLT PTSVLDYFGT ESVQRSGKKM VTSKRKESSQ NTEDSRLNDE
210 220 230 240 250
AIAKQLQLDE DAELERQLHE DEEFARTLAL LDEEPKIKKA RKDSEEGEES
260 270 280 290 300
FSSVQDDLSK AEKQKSPNKA ELFSTARKTY SPAKHGKGRA SEDAKQPCKS
310 320 330 340 350
AHRKEACSSP KASAKLALMK AKEESSYNET ELLAARRKES ATEPKGEKTT
360 370 380 390 400
PKKTKVSPTK RESVSPEDSE KKRTNYQAYR SYLNREGPKA LGSKEIPKGA
410 420 430 440 450
ENCLEGLTFV ITGVLESIER DEAKSLIERY GGKVTGNVSK KTNYLVMGRD
460 470 480 490 500
SGQSKSDKAA ALGTKILDED GLLDLIRTMP GKRSKYEMAA EAEMKKEKSK
510 520 530 540 550
LERTPQKNDQ GKRKISPAKK ESESKKCKLT LLKNSPMKAV KKEASTCPRG
560 570 580 590 600
LDVKETHGNR SSNKEECLLW VDKYKPASLK NIIGQQGDQS CANKLLRWLR
610 620 630 640 650
NWHKSSPEEK KHAAKFGKLA SKDDGSSFKA ALLSGPPGVG KTTTASLVCQ
660 670 680 690 700
ELGYSYVELN ASDTRSKNSL KAVVAESLNN TSIKGFYTSG AAPSVSARHA
710 720 730 740 750
LIMDEVDGMA GNEDRGGIQE LIGLIKHTKI PIICMCNDRN HPKIRSLVHY
760 770 780 790 800
CFDLRFQRPR VEQIKSAMLS IAFKEGLKIP PPAMNEIILG ANQDVRQVLH
810 820 830 840 850
NLSMWCAQSK ALTYDQAKAD SQRAKKDIRL GPFDVTRKVF AAGEETAHMS
860 870 880 890 900
LMDKSDLFFH DYSIAPLFVQ ENYLHVKPVA AGGDMKKHLM LLSRAADSIC
910 920 930 940 950
DGDLVDNQIR SKQNWSLLPT QAIYASVLPG ELMRGYMTQF PSFPSWLGKH
960 970 980 990 1000
SSTGKHDRIV QDLSLHMSLR TYSSKRTVNM DYLSHIRDAL VRPLTSQGVE
1010 1020 1030 1040 1050
GAQHVIKLMD TYYLMKEDFE NIMEVSSWGG KPSAFSKLDP KVKAAFTRAY
1060 1070 1080 1090 1100
NKEAHLTPYS LQVVKTSRLS TGPALDSEYS EEFQEDDTQS EKEQDAVETD
1110 1120 1130
AMIKKKTRSS KPSKSEREKE SKKGKGKNWK K
Length:1,131
Mass (Da):125,985
Last modified:October 1, 1996 - v2
Checksum:iA6F4F970A7F9EE94
GO
Isoform 2 (identifier: P35601-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     614-614: Missing.

Note: Alternative use of an acceptor site.

Show »
Length:1,130
Mass (Da):125,914
Checksum:i4EF6B8B2B58F1EDA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661Y → N in AAA79698. (PubMed:7659092)Curated
Sequence conflicti187 – 1871E → EPDFCLSCLIFFGIQ in AAC52140. 1 PublicationCurated
Sequence conflicti254 – 2541V → A in AAB60452. 1 PublicationCurated
Sequence conflicti559 – 5591N → S in AAC52140. 1 PublicationCurated
Sequence conflicti945 – 9451S → N in AAA21643. (PubMed:8265586)Curated
Sequence conflicti1071 – 10711T → A in AAA79698. (PubMed:7659092)Curated
Sequence conflicti1104 – 11041K → KQ in AAC52140. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei614 – 6141Missing in isoform 2. 2 PublicationsVSP_008444

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01222 mRNA. Translation: AAA21643.1.
X72711 mRNA. Translation: CAA51260.1.
U36441 mRNA. Translation: AAA79698.1.
U07157 mRNA. Translation: AAC52140.1.
U15037 mRNA. Translation: AAB60452.1.
CCDSiCCDS39096.1. [P35601-1]
PIRiA49393.
RefSeqiNP_035388.2. NM_011258.2.
UniGeneiMm.148877.

Genome annotation databases

GeneIDi19687.
KEGGimmu:19687.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01222 mRNA. Translation: AAA21643.1 .
X72711 mRNA. Translation: CAA51260.1 .
U36441 mRNA. Translation: AAA79698.1 .
U07157 mRNA. Translation: AAC52140.1 .
U15037 mRNA. Translation: AAB60452.1 .
CCDSi CCDS39096.1. [P35601-1 ]
PIRi A49393.
RefSeqi NP_035388.2. NM_011258.2.
UniGenei Mm.148877.

3D structure databases

ProteinModelPortali P35601.
SMRi P35601. Positions 372-479, 570-829.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202846. 6 interactions.
MINTi MINT-4084942.

PTM databases

PhosphoSitei P35601.

Proteomic databases

MaxQBi P35601.
PaxDbi P35601.
PRIDEi P35601.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 19687.
KEGGi mmu:19687.

Organism-specific databases

CTDi 5981.
MGIi MGI:97891. Rfc1.

Phylogenomic databases

eggNOGi COG5275.
HOVERGENi HBG004167.
InParanoidi P35601.
KOi K10754.
PhylomeDBi P35601.

Miscellaneous databases

ChiTaRSi Rfc1. mouse.
NextBioi 297020.
PROi P35601.
SOURCEi Search...

Gene expression databases

CleanExi MM_RFC1.
Genevestigatori P35601.

Family and domain databases

Gene3Di 3.40.50.10190. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001357. BRCT_dom.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR012178. DNA_replication_fac_C_lsu.
IPR013725. DNA_replication_fac_RFC1_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00004. AAA. 1 hit.
PF00533. BRCT. 1 hit.
PF08519. RFC1. 1 hit.
[Graphical view ]
PIRSFi PIRSF036578. RFC1. 1 hit.
SMARTi SM00382. AAA. 1 hit.
SM00292. BRCT. 1 hit.
[Graphical view ]
SUPFAMi SSF48019. SSF48019. 1 hit.
SSF52113. SSF52113. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50172. BRCT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the large subunit of activator 1 (replication factor C) reveals homology with bacterial DNA ligases."
    Burbelo P.D., Utani A., Pan Z., Yamada Y.
    Proc. Natl. Acad. Sci. U.S.A. 90:11543-11547(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: BALB/c.
  2. "Cloning, expression, and chromosomal localization of the 140-kilodalton subunit of replication factor C from mice and humans."
    Luckow B., Bunz F., Stillman B., Lichter P., Schuetz G.
    Mol. Cell. Biol. 14:1626-1634(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Liver.
  3. "Differentiation-specific element binding protein (DSEB) binds to a defined element in the promoter of the angiotensinogen gene required for the irreversible induction of gene expression during differentiation of 3T3-L1 adipoblasts to adipocytes."
    McGehee Habener J.F.
    Mol. Endocrinol. 9:487-501(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: Swiss.
  4. Haque S.J.
    Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  5. Lossie A.C., Haugen B.H., Wood W.M., Camper S.A., Gordon D.F.
    Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-565.
    Strain: LAF1.
  6. "Evaluation of inter- and intramolecular primary structure homologies of interferons by a Monte Carlo method."
    Haque S.J., Kumar A., Fischer T., Rutherford M.N., Williams B.R.
    J. Interferon Res. 10:31-38(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 354-528.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-70; SER-107 AND THR-109, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-244, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiRFC1_MOUSE
AccessioniPrimary (citable) accession number: P35601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3