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P35601 (RFC1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replication factor C subunit 1
Alternative name(s):
A1-P145
Activator 1 140 kDa subunit
Short name=A1 140 kDa subunit
Activator 1 large subunit
Activator 1 subunit 1
Differentiation-specific element-binding protein
ISRE-binding protein
Replication factor C 140 kDa subunit
Short name=RF-C 140 kDa subunit
Short name=RFC140
Replication factor C large subunit
Gene names
Name:Rfc1
Synonyms:Ibf-1, Recc1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length1131 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction.

Subunit structure

Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA By similarity.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the activator 1 large subunit family.

Contains 1 BRCT domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P35601-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P35601-2)

The sequence of this isoform differs from the canonical sequence as follows:
     614-614: Missing.
Note: Alternative use of an acceptor site.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11311131Replication factor C subunit 1
PRO_0000121773

Regions

Domain399 – 48991BRCT
Nucleotide binding635 – 6428ATP By similarity
Region354 – 528175Interferon-stimulated-response-element binding region
Motif1104 – 11085Nuclear localization signal Potential

Amino acid modifications

Modified residue661Phosphotyrosine By similarity
Modified residue681Phosphoserine Ref.7
Modified residue701Phosphoserine Ref.7
Modified residue721Phosphoserine By similarity
Modified residue1071Phosphoserine Ref.7
Modified residue1091Phosphothreonine Ref.7
Modified residue1551Phosphoserine By similarity
Modified residue1601Phosphothreonine By similarity
Modified residue1621Phosphothreonine By similarity
Modified residue1631Phosphoserine By similarity
Modified residue1721Phosphoserine By similarity
Modified residue1891Phosphoserine By similarity
Modified residue2441Phosphoserine Ref.7 Ref.8 Ref.9
Modified residue2811Phosphoserine By similarity
Modified residue3091Phosphoserine By similarity
Modified residue3651Phosphoserine By similarity
Modified residue10901Phosphoserine By similarity

Natural variations

Alternative sequence6141Missing in isoform 2.
VSP_008444

Experimental info

Sequence conflict661Y → N in AAA79698. Ref.3
Sequence conflict1871E → EPDFCLSCLIFFGIQ in AAC52140. Ref.4
Sequence conflict2541V → A in AAB60452. Ref.5
Sequence conflict5591N → S in AAC52140. Ref.4
Sequence conflict9451S → N in AAA21643. Ref.1
Sequence conflict10711T → A in AAA79698. Ref.3
Sequence conflict11041K → KQ in AAC52140. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: A6F4F970A7F9EE94

FASTA1,131125,985
        10         20         30         40         50         60 
MDIRKFFGVI SSGKKPVNET VKNEKTKASE GTVKGKKGVK EAKVNNSGKE DASKPKQHSK 

        70         80         90        100        110        120 
KKRIIYDSDS ESEETVQVKN AKKKSEKLSL SYKPGKVSQK DPVTYVSETD EDDDFVCKKA 

       130        140        150        160        170        180 
ASKSKENGVS TNSYLGTSNV KKNEENVKTK NKPLSPIKLT PTSVLDYFGT ESVQRSGKKM 

       190        200        210        220        230        240 
VTSKRKESSQ NTEDSRLNDE AIAKQLQLDE DAELERQLHE DEEFARTLAL LDEEPKIKKA 

       250        260        270        280        290        300 
RKDSEEGEES FSSVQDDLSK AEKQKSPNKA ELFSTARKTY SPAKHGKGRA SEDAKQPCKS 

       310        320        330        340        350        360 
AHRKEACSSP KASAKLALMK AKEESSYNET ELLAARRKES ATEPKGEKTT PKKTKVSPTK 

       370        380        390        400        410        420 
RESVSPEDSE KKRTNYQAYR SYLNREGPKA LGSKEIPKGA ENCLEGLTFV ITGVLESIER 

       430        440        450        460        470        480 
DEAKSLIERY GGKVTGNVSK KTNYLVMGRD SGQSKSDKAA ALGTKILDED GLLDLIRTMP 

       490        500        510        520        530        540 
GKRSKYEMAA EAEMKKEKSK LERTPQKNDQ GKRKISPAKK ESESKKCKLT LLKNSPMKAV 

       550        560        570        580        590        600 
KKEASTCPRG LDVKETHGNR SSNKEECLLW VDKYKPASLK NIIGQQGDQS CANKLLRWLR 

       610        620        630        640        650        660 
NWHKSSPEEK KHAAKFGKLA SKDDGSSFKA ALLSGPPGVG KTTTASLVCQ ELGYSYVELN 

       670        680        690        700        710        720 
ASDTRSKNSL KAVVAESLNN TSIKGFYTSG AAPSVSARHA LIMDEVDGMA GNEDRGGIQE 

       730        740        750        760        770        780 
LIGLIKHTKI PIICMCNDRN HPKIRSLVHY CFDLRFQRPR VEQIKSAMLS IAFKEGLKIP 

       790        800        810        820        830        840 
PPAMNEIILG ANQDVRQVLH NLSMWCAQSK ALTYDQAKAD SQRAKKDIRL GPFDVTRKVF 

       850        860        870        880        890        900 
AAGEETAHMS LMDKSDLFFH DYSIAPLFVQ ENYLHVKPVA AGGDMKKHLM LLSRAADSIC 

       910        920        930        940        950        960 
DGDLVDNQIR SKQNWSLLPT QAIYASVLPG ELMRGYMTQF PSFPSWLGKH SSTGKHDRIV 

       970        980        990       1000       1010       1020 
QDLSLHMSLR TYSSKRTVNM DYLSHIRDAL VRPLTSQGVE GAQHVIKLMD TYYLMKEDFE 

      1030       1040       1050       1060       1070       1080 
NIMEVSSWGG KPSAFSKLDP KVKAAFTRAY NKEAHLTPYS LQVVKTSRLS TGPALDSEYS 

      1090       1100       1110       1120       1130 
EEFQEDDTQS EKEQDAVETD AMIKKKTRSS KPSKSEREKE SKKGKGKNWK K

« Hide

Isoform 2 [UniParc].

Checksum: 4EF6B8B2B58F1EDA
Show »

FASTA1,130125,914

References

« Hide 'large scale' references
[1]"Cloning of the large subunit of activator 1 (replication factor C) reveals homology with bacterial DNA ligases."
Burbelo P.D., Utani A., Pan Z., Yamada Y.
Proc. Natl. Acad. Sci. U.S.A. 90:11543-11547(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: BALB/c.
[2]"Cloning, expression, and chromosomal localization of the 140-kilodalton subunit of replication factor C from mice and humans."
Luckow B., Bunz F., Stillman B., Lichter P., Schuetz G.
Mol. Cell. Biol. 14:1626-1634(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Liver.
[3]"Differentiation-specific element binding protein (DSEB) binds to a defined element in the promoter of the angiotensinogen gene required for the irreversible induction of gene expression during differentiation of 3T3-L1 adipoblasts to adipocytes."
McGehee Habener J.F.
Mol. Endocrinol. 9:487-501(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: Swiss.
[4]Haque S.J.
Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[5]Lossie A.C., Haugen B.H., Wood W.M., Camper S.A., Gordon D.F.
Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-565.
Strain: LAF1.
[6]"Evaluation of inter- and intramolecular primary structure homologies of interferons by a Monte Carlo method."
Haque S.J., Kumar A., Fischer T., Rutherford M.N., Williams B.R.
J. Interferon Res. 10:31-38(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 354-528.
[7]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-70; SER-107; THR-109 AND SER-244, MASS SPECTROMETRY.
Tissue: Liver.
[8]"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry."
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M.
J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-244, MASS SPECTROMETRY.
Tissue: Melanoma.
[9]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-244, MASS SPECTROMETRY.
Tissue: Macrophage.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U01222 mRNA. Translation: AAA21643.1.
X72711 mRNA. Translation: CAA51260.1.
U36441 mRNA. Translation: AAA79698.1.
U07157 mRNA. Translation: AAC52140.1.
U15037 mRNA. Translation: AAB60452.1.
IPIIPI00378168.
IPI00896013.
PIRA49393.
RefSeqNP_035388.2. NM_011258.2.
UniGeneMm.148877.

3D structure databases

ProteinModelPortalP35601.
SMRP35601. Positions 372-479, 570-829.
ModBaseSearch...

Protein-protein interaction databases

MINTMINT-4084942.

PTM databases

PhosphoSiteP35601.

Proteomic databases

PaxDbP35601.
PRIDEP35601.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID19687.
KEGGmmu:19687.

Organism-specific databases

CTD5981.
MGIMGI:97891. Rfc1.

Phylogenomic databases

eggNOGCOG5275.
HOVERGENHBG004167.
InParanoidP35601.
KOK10754.
OrthoDBEOG4D26P3.

Gene expression databases

CleanExMM_RFC1.
GenevestigatorP35601.
GermOnlineENSMUSG00000029191. Mus musculus.

Family and domain databases

Gene3D3.40.50.10190. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001357. BRCT_dom.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR012178. DNA_replication_fac_C_lsu.
IPR013725. DNA_replication_fac_RFC1_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00004. AAA. 1 hit.
PF00533. BRCT. 1 hit.
PF08519. RFC1. 1 hit.
[Graphical view]
PIRSFPIRSF036578. RFC1. 1 hit.
SMARTSM00382. AAA. 1 hit.
SM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMSSF52113. BRCT. 1 hit.
SSF48019. Pol_clamp_load_C. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRFC1. mouse.
NextBio297020.
SOURCESearch...

Entry information

Entry nameRFC1_MOUSE
AccessionPrimary (citable) accession number: P35601
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1996
Last modified: May 29, 2013
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents