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P35601

- RFC1_MOUSE

UniProt

P35601 - RFC1_MOUSE

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Protein

Replication factor C subunit 1

Gene
Rfc1, Ibf-1, Recc1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi635 – 6428ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: RefGenome
  3. DNA clamp loader activity Source: InterPro

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

DNA replication, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C subunit 1
Alternative name(s):
A1-P145
Activator 1 140 kDa subunit
Short name:
A1 140 kDa subunit
Activator 1 large subunit
Activator 1 subunit 1
Differentiation-specific element-binding protein
ISRE-binding protein
Replication factor C 140 kDa subunit
Short name:
RF-C 140 kDa subunit
Short name:
RFC140
Replication factor C large subunit
Gene namesi
Name:Rfc1
Synonyms:Ibf-1, Recc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:97891. Rfc1.

Subcellular locationi

GO - Cellular componenti

  1. DNA replication factor C complex Source: InterPro
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11311131Replication factor C subunit 1PRO_0000121773Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei66 – 661Phosphotyrosine By similarity
Modified residuei68 – 681Phosphoserine1 Publication
Modified residuei70 – 701Phosphoserine1 Publication
Modified residuei72 – 721Phosphoserine By similarity
Modified residuei107 – 1071Phosphoserine1 Publication
Modified residuei109 – 1091Phosphothreonine1 Publication
Modified residuei155 – 1551Phosphoserine By similarity
Modified residuei160 – 1601Phosphothreonine By similarity
Modified residuei162 – 1621Phosphothreonine By similarity
Modified residuei163 – 1631Phosphoserine By similarity
Modified residuei172 – 1721Phosphoserine By similarity
Modified residuei189 – 1891Phosphoserine By similarity
Modified residuei244 – 2441Phosphoserine1 Publication
Modified residuei281 – 2811Phosphoserine By similarity
Modified residuei309 – 3091Phosphoserine By similarity
Modified residuei365 – 3651Phosphoserine By similarity
Modified residuei1090 – 10901Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP35601.
PRIDEiP35601.

PTM databases

PhosphoSiteiP35601.

Expressioni

Gene expression databases

CleanExiMM_RFC1.
GenevestigatoriP35601.

Interactioni

Subunit structurei

Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA By similarity.

Protein-protein interaction databases

BioGridi202846. 6 interactions.
MINTiMINT-4084942.

Structurei

3D structure databases

ProteinModelPortaliP35601.
SMRiP35601. Positions 372-479, 570-829.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini399 – 48991BRCTAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni354 – 528175Interferon-stimulated-response-element binding regionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1104 – 11085Nuclear localization signal Reviewed prediction

Sequence similaritiesi

Contains 1 BRCT domain.

Phylogenomic databases

eggNOGiCOG5275.
HOVERGENiHBG004167.
InParanoidiP35601.
KOiK10754.
PhylomeDBiP35601.

Family and domain databases

Gene3Di3.40.50.10190. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001357. BRCT_dom.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR012178. DNA_replication_fac_C_lsu.
IPR013725. DNA_replication_fac_RFC1_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF00533. BRCT. 1 hit.
PF08519. RFC1. 1 hit.
[Graphical view]
PIRSFiPIRSF036578. RFC1. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52113. SSF52113. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P35601-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDIRKFFGVI SSGKKPVNET VKNEKTKASE GTVKGKKGVK EAKVNNSGKE     50
DASKPKQHSK KKRIIYDSDS ESEETVQVKN AKKKSEKLSL SYKPGKVSQK 100
DPVTYVSETD EDDDFVCKKA ASKSKENGVS TNSYLGTSNV KKNEENVKTK 150
NKPLSPIKLT PTSVLDYFGT ESVQRSGKKM VTSKRKESSQ NTEDSRLNDE 200
AIAKQLQLDE DAELERQLHE DEEFARTLAL LDEEPKIKKA RKDSEEGEES 250
FSSVQDDLSK AEKQKSPNKA ELFSTARKTY SPAKHGKGRA SEDAKQPCKS 300
AHRKEACSSP KASAKLALMK AKEESSYNET ELLAARRKES ATEPKGEKTT 350
PKKTKVSPTK RESVSPEDSE KKRTNYQAYR SYLNREGPKA LGSKEIPKGA 400
ENCLEGLTFV ITGVLESIER DEAKSLIERY GGKVTGNVSK KTNYLVMGRD 450
SGQSKSDKAA ALGTKILDED GLLDLIRTMP GKRSKYEMAA EAEMKKEKSK 500
LERTPQKNDQ GKRKISPAKK ESESKKCKLT LLKNSPMKAV KKEASTCPRG 550
LDVKETHGNR SSNKEECLLW VDKYKPASLK NIIGQQGDQS CANKLLRWLR 600
NWHKSSPEEK KHAAKFGKLA SKDDGSSFKA ALLSGPPGVG KTTTASLVCQ 650
ELGYSYVELN ASDTRSKNSL KAVVAESLNN TSIKGFYTSG AAPSVSARHA 700
LIMDEVDGMA GNEDRGGIQE LIGLIKHTKI PIICMCNDRN HPKIRSLVHY 750
CFDLRFQRPR VEQIKSAMLS IAFKEGLKIP PPAMNEIILG ANQDVRQVLH 800
NLSMWCAQSK ALTYDQAKAD SQRAKKDIRL GPFDVTRKVF AAGEETAHMS 850
LMDKSDLFFH DYSIAPLFVQ ENYLHVKPVA AGGDMKKHLM LLSRAADSIC 900
DGDLVDNQIR SKQNWSLLPT QAIYASVLPG ELMRGYMTQF PSFPSWLGKH 950
SSTGKHDRIV QDLSLHMSLR TYSSKRTVNM DYLSHIRDAL VRPLTSQGVE 1000
GAQHVIKLMD TYYLMKEDFE NIMEVSSWGG KPSAFSKLDP KVKAAFTRAY 1050
NKEAHLTPYS LQVVKTSRLS TGPALDSEYS EEFQEDDTQS EKEQDAVETD 1100
AMIKKKTRSS KPSKSEREKE SKKGKGKNWK K 1131
Length:1,131
Mass (Da):125,985
Last modified:October 1, 1996 - v2
Checksum:iA6F4F970A7F9EE94
GO
Isoform 2 (identifier: P35601-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     614-614: Missing.

Note: Alternative use of an acceptor site.

Show »
Length:1,130
Mass (Da):125,914
Checksum:i4EF6B8B2B58F1EDA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei614 – 6141Missing in isoform 2. VSP_008444

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661Y → N in AAA79698. 1 Publication
Sequence conflicti187 – 1871E → EPDFCLSCLIFFGIQ in AAC52140. 1 Publication
Sequence conflicti254 – 2541V → A in AAB60452. 1 Publication
Sequence conflicti559 – 5591N → S in AAC52140. 1 Publication
Sequence conflicti945 – 9451S → N in AAA21643. 1 Publication
Sequence conflicti1071 – 10711T → A in AAA79698. 1 Publication
Sequence conflicti1104 – 11041K → KQ in AAC52140. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U01222 mRNA. Translation: AAA21643.1.
X72711 mRNA. Translation: CAA51260.1.
U36441 mRNA. Translation: AAA79698.1.
U07157 mRNA. Translation: AAC52140.1.
U15037 mRNA. Translation: AAB60452.1.
CCDSiCCDS39096.1. [P35601-1]
PIRiA49393.
RefSeqiNP_035388.2. NM_011258.2.
UniGeneiMm.148877.

Genome annotation databases

GeneIDi19687.
KEGGimmu:19687.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U01222 mRNA. Translation: AAA21643.1 .
X72711 mRNA. Translation: CAA51260.1 .
U36441 mRNA. Translation: AAA79698.1 .
U07157 mRNA. Translation: AAC52140.1 .
U15037 mRNA. Translation: AAB60452.1 .
CCDSi CCDS39096.1. [P35601-1 ]
PIRi A49393.
RefSeqi NP_035388.2. NM_011258.2.
UniGenei Mm.148877.

3D structure databases

ProteinModelPortali P35601.
SMRi P35601. Positions 372-479, 570-829.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202846. 6 interactions.
MINTi MINT-4084942.

PTM databases

PhosphoSitei P35601.

Proteomic databases

PaxDbi P35601.
PRIDEi P35601.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 19687.
KEGGi mmu:19687.

Organism-specific databases

CTDi 5981.
MGIi MGI:97891. Rfc1.

Phylogenomic databases

eggNOGi COG5275.
HOVERGENi HBG004167.
InParanoidi P35601.
KOi K10754.
PhylomeDBi P35601.

Miscellaneous databases

ChiTaRSi RFC1. mouse.
NextBioi 297020.
PROi P35601.
SOURCEi Search...

Gene expression databases

CleanExi MM_RFC1.
Genevestigatori P35601.

Family and domain databases

Gene3Di 3.40.50.10190. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001357. BRCT_dom.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR012178. DNA_replication_fac_C_lsu.
IPR013725. DNA_replication_fac_RFC1_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00004. AAA. 1 hit.
PF00533. BRCT. 1 hit.
PF08519. RFC1. 1 hit.
[Graphical view ]
PIRSFi PIRSF036578. RFC1. 1 hit.
SMARTi SM00382. AAA. 1 hit.
SM00292. BRCT. 1 hit.
[Graphical view ]
SUPFAMi SSF48019. SSF48019. 1 hit.
SSF52113. SSF52113. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50172. BRCT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the large subunit of activator 1 (replication factor C) reveals homology with bacterial DNA ligases."
    Burbelo P.D., Utani A., Pan Z., Yamada Y.
    Proc. Natl. Acad. Sci. U.S.A. 90:11543-11547(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: BALB/c.
  2. "Cloning, expression, and chromosomal localization of the 140-kilodalton subunit of replication factor C from mice and humans."
    Luckow B., Bunz F., Stillman B., Lichter P., Schuetz G.
    Mol. Cell. Biol. 14:1626-1634(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Liver.
  3. "Differentiation-specific element binding protein (DSEB) binds to a defined element in the promoter of the angiotensinogen gene required for the irreversible induction of gene expression during differentiation of 3T3-L1 adipoblasts to adipocytes."
    McGehee Habener J.F.
    Mol. Endocrinol. 9:487-501(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: Swiss.
  4. Haque S.J.
    Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  5. Lossie A.C., Haugen B.H., Wood W.M., Camper S.A., Gordon D.F.
    Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-565.
    Strain: LAF1.
  6. "Evaluation of inter- and intramolecular primary structure homologies of interferons by a Monte Carlo method."
    Haque S.J., Kumar A., Fischer T., Rutherford M.N., Williams B.R.
    J. Interferon Res. 10:31-38(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 354-528.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-70; SER-107 AND THR-109, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-244, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiRFC1_MOUSE
AccessioniPrimary (citable) accession number: P35601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi