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P35601

- RFC1_MOUSE

UniProt

P35601 - RFC1_MOUSE

Protein

Replication factor C subunit 1

Gene

Rfc1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi635 – 6428ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: RefGenome
    3. DNA clamp loader activity Source: InterPro

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    DNA replication, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replication factor C subunit 1
    Alternative name(s):
    A1-P145
    Activator 1 140 kDa subunit
    Short name:
    A1 140 kDa subunit
    Activator 1 large subunit
    Activator 1 subunit 1
    Differentiation-specific element-binding protein
    ISRE-binding protein
    Replication factor C 140 kDa subunit
    Short name:
    RF-C 140 kDa subunit
    Short name:
    RFC140
    Replication factor C large subunit
    Gene namesi
    Name:Rfc1
    Synonyms:Ibf-1, Recc1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:97891. Rfc1.

    Subcellular locationi

    GO - Cellular componenti

    1. DNA replication factor C complex Source: InterPro
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11311131Replication factor C subunit 1PRO_0000121773Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei66 – 661PhosphotyrosineBy similarity
    Modified residuei68 – 681Phosphoserine1 Publication
    Modified residuei70 – 701Phosphoserine1 Publication
    Modified residuei72 – 721PhosphoserineBy similarity
    Modified residuei107 – 1071Phosphoserine1 Publication
    Modified residuei109 – 1091Phosphothreonine1 Publication
    Modified residuei155 – 1551PhosphoserineBy similarity
    Modified residuei160 – 1601PhosphothreonineBy similarity
    Modified residuei162 – 1621PhosphothreonineBy similarity
    Modified residuei163 – 1631PhosphoserineBy similarity
    Modified residuei172 – 1721PhosphoserineBy similarity
    Modified residuei189 – 1891PhosphoserineBy similarity
    Modified residuei244 – 2441Phosphoserine1 Publication
    Modified residuei281 – 2811PhosphoserineBy similarity
    Modified residuei309 – 3091PhosphoserineBy similarity
    Modified residuei365 – 3651PhosphoserineBy similarity
    Modified residuei1090 – 10901PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP35601.
    PRIDEiP35601.

    PTM databases

    PhosphoSiteiP35601.

    Expressioni

    Gene expression databases

    CleanExiMM_RFC1.
    GenevestigatoriP35601.

    Interactioni

    Subunit structurei

    Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA By similarity.By similarity

    Protein-protein interaction databases

    BioGridi202846. 6 interactions.
    MINTiMINT-4084942.

    Structurei

    3D structure databases

    ProteinModelPortaliP35601.
    SMRiP35601. Positions 372-479, 570-829.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini399 – 48991BRCTPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni354 – 528175Interferon-stimulated-response-element binding regionAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1104 – 11085Nuclear localization signalSequence Analysis

    Sequence similaritiesi

    Belongs to the activator 1 large subunit family.Curated
    Contains 1 BRCT domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5275.
    HOVERGENiHBG004167.
    InParanoidiP35601.
    KOiK10754.
    PhylomeDBiP35601.

    Family and domain databases

    Gene3Di3.40.50.10190. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR001357. BRCT_dom.
    IPR008921. DNA_pol3_clamp-load_cplx_C.
    IPR012178. DNA_replication_fac_C_lsu.
    IPR013725. DNA_replication_fac_RFC1_C.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    PF00533. BRCT. 1 hit.
    PF08519. RFC1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036578. RFC1. 1 hit.
    SMARTiSM00382. AAA. 1 hit.
    SM00292. BRCT. 1 hit.
    [Graphical view]
    SUPFAMiSSF48019. SSF48019. 1 hit.
    SSF52113. SSF52113. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS50172. BRCT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P35601-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDIRKFFGVI SSGKKPVNET VKNEKTKASE GTVKGKKGVK EAKVNNSGKE     50
    DASKPKQHSK KKRIIYDSDS ESEETVQVKN AKKKSEKLSL SYKPGKVSQK 100
    DPVTYVSETD EDDDFVCKKA ASKSKENGVS TNSYLGTSNV KKNEENVKTK 150
    NKPLSPIKLT PTSVLDYFGT ESVQRSGKKM VTSKRKESSQ NTEDSRLNDE 200
    AIAKQLQLDE DAELERQLHE DEEFARTLAL LDEEPKIKKA RKDSEEGEES 250
    FSSVQDDLSK AEKQKSPNKA ELFSTARKTY SPAKHGKGRA SEDAKQPCKS 300
    AHRKEACSSP KASAKLALMK AKEESSYNET ELLAARRKES ATEPKGEKTT 350
    PKKTKVSPTK RESVSPEDSE KKRTNYQAYR SYLNREGPKA LGSKEIPKGA 400
    ENCLEGLTFV ITGVLESIER DEAKSLIERY GGKVTGNVSK KTNYLVMGRD 450
    SGQSKSDKAA ALGTKILDED GLLDLIRTMP GKRSKYEMAA EAEMKKEKSK 500
    LERTPQKNDQ GKRKISPAKK ESESKKCKLT LLKNSPMKAV KKEASTCPRG 550
    LDVKETHGNR SSNKEECLLW VDKYKPASLK NIIGQQGDQS CANKLLRWLR 600
    NWHKSSPEEK KHAAKFGKLA SKDDGSSFKA ALLSGPPGVG KTTTASLVCQ 650
    ELGYSYVELN ASDTRSKNSL KAVVAESLNN TSIKGFYTSG AAPSVSARHA 700
    LIMDEVDGMA GNEDRGGIQE LIGLIKHTKI PIICMCNDRN HPKIRSLVHY 750
    CFDLRFQRPR VEQIKSAMLS IAFKEGLKIP PPAMNEIILG ANQDVRQVLH 800
    NLSMWCAQSK ALTYDQAKAD SQRAKKDIRL GPFDVTRKVF AAGEETAHMS 850
    LMDKSDLFFH DYSIAPLFVQ ENYLHVKPVA AGGDMKKHLM LLSRAADSIC 900
    DGDLVDNQIR SKQNWSLLPT QAIYASVLPG ELMRGYMTQF PSFPSWLGKH 950
    SSTGKHDRIV QDLSLHMSLR TYSSKRTVNM DYLSHIRDAL VRPLTSQGVE 1000
    GAQHVIKLMD TYYLMKEDFE NIMEVSSWGG KPSAFSKLDP KVKAAFTRAY 1050
    NKEAHLTPYS LQVVKTSRLS TGPALDSEYS EEFQEDDTQS EKEQDAVETD 1100
    AMIKKKTRSS KPSKSEREKE SKKGKGKNWK K 1131
    Length:1,131
    Mass (Da):125,985
    Last modified:October 1, 1996 - v2
    Checksum:iA6F4F970A7F9EE94
    GO
    Isoform 2 (identifier: P35601-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         614-614: Missing.

    Note: Alternative use of an acceptor site.

    Show »
    Length:1,130
    Mass (Da):125,914
    Checksum:i4EF6B8B2B58F1EDA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti66 – 661Y → N in AAA79698. (PubMed:7659092)Curated
    Sequence conflicti187 – 1871E → EPDFCLSCLIFFGIQ in AAC52140. 1 PublicationCurated
    Sequence conflicti254 – 2541V → A in AAB60452. 1 PublicationCurated
    Sequence conflicti559 – 5591N → S in AAC52140. 1 PublicationCurated
    Sequence conflicti945 – 9451S → N in AAA21643. (PubMed:8265586)Curated
    Sequence conflicti1071 – 10711T → A in AAA79698. (PubMed:7659092)Curated
    Sequence conflicti1104 – 11041K → KQ in AAC52140. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei614 – 6141Missing in isoform 2. 2 PublicationsVSP_008444

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U01222 mRNA. Translation: AAA21643.1.
    X72711 mRNA. Translation: CAA51260.1.
    U36441 mRNA. Translation: AAA79698.1.
    U07157 mRNA. Translation: AAC52140.1.
    U15037 mRNA. Translation: AAB60452.1.
    CCDSiCCDS39096.1. [P35601-1]
    PIRiA49393.
    RefSeqiNP_035388.2. NM_011258.2.
    UniGeneiMm.148877.

    Genome annotation databases

    GeneIDi19687.
    KEGGimmu:19687.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U01222 mRNA. Translation: AAA21643.1 .
    X72711 mRNA. Translation: CAA51260.1 .
    U36441 mRNA. Translation: AAA79698.1 .
    U07157 mRNA. Translation: AAC52140.1 .
    U15037 mRNA. Translation: AAB60452.1 .
    CCDSi CCDS39096.1. [P35601-1 ]
    PIRi A49393.
    RefSeqi NP_035388.2. NM_011258.2.
    UniGenei Mm.148877.

    3D structure databases

    ProteinModelPortali P35601.
    SMRi P35601. Positions 372-479, 570-829.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202846. 6 interactions.
    MINTi MINT-4084942.

    PTM databases

    PhosphoSitei P35601.

    Proteomic databases

    PaxDbi P35601.
    PRIDEi P35601.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 19687.
    KEGGi mmu:19687.

    Organism-specific databases

    CTDi 5981.
    MGIi MGI:97891. Rfc1.

    Phylogenomic databases

    eggNOGi COG5275.
    HOVERGENi HBG004167.
    InParanoidi P35601.
    KOi K10754.
    PhylomeDBi P35601.

    Miscellaneous databases

    ChiTaRSi RFC1. mouse.
    NextBioi 297020.
    PROi P35601.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_RFC1.
    Genevestigatori P35601.

    Family and domain databases

    Gene3Di 3.40.50.10190. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR001357. BRCT_dom.
    IPR008921. DNA_pol3_clamp-load_cplx_C.
    IPR012178. DNA_replication_fac_C_lsu.
    IPR013725. DNA_replication_fac_RFC1_C.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    PF00533. BRCT. 1 hit.
    PF08519. RFC1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036578. RFC1. 1 hit.
    SMARTi SM00382. AAA. 1 hit.
    SM00292. BRCT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48019. SSF48019. 1 hit.
    SSF52113. SSF52113. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS50172. BRCT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the large subunit of activator 1 (replication factor C) reveals homology with bacterial DNA ligases."
      Burbelo P.D., Utani A., Pan Z., Yamada Y.
      Proc. Natl. Acad. Sci. U.S.A. 90:11543-11547(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: BALB/c.
    2. "Cloning, expression, and chromosomal localization of the 140-kilodalton subunit of replication factor C from mice and humans."
      Luckow B., Bunz F., Stillman B., Lichter P., Schuetz G.
      Mol. Cell. Biol. 14:1626-1634(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Liver.
    3. "Differentiation-specific element binding protein (DSEB) binds to a defined element in the promoter of the angiotensinogen gene required for the irreversible induction of gene expression during differentiation of 3T3-L1 adipoblasts to adipocytes."
      McGehee Habener J.F.
      Mol. Endocrinol. 9:487-501(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: Swiss.
    4. Haque S.J.
      Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    5. Lossie A.C., Haugen B.H., Wood W.M., Camper S.A., Gordon D.F.
      Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-565.
      Strain: LAF1.
    6. "Evaluation of inter- and intramolecular primary structure homologies of interferons by a Monte Carlo method."
      Haque S.J., Kumar A., Fischer T., Rutherford M.N., Williams B.R.
      J. Interferon Res. 10:31-38(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 354-528.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-70; SER-107 AND THR-109, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    8. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-244, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiRFC1_MOUSE
    AccessioniPrimary (citable) accession number: P35601
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 133 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3