Reviewed, UniProtKB/Swiss-Prot P35596 (GLPO_STRPN)

Names and origin

Protein names

Recommended name:
    Alpha-glycerophosphate oxidase
    EC=1.1.3.21
Alternative name(s):
    Glycerol-3-phosphate oxidase
    Exported protein 6

Gene names

Name:	glpO
Synonyms:	exp6
Ordered Locus Names:	SP_2185

Organism

Streptococcus pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + O2 = glycerone phosphate + H2O2.
Cofactor	FAD.
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate dehydrogenase activity Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 608	608	Alpha-glycerophosphate oxidase		PRO_0000126108
Regions
	Nucleotide binding	21 – 49	29	FAD Potential
Experimental info
	Sequence conflict	100	1	S → P in AAC34740. Ref.1
	Sequence conflict	138	1	A → T in AAC34740. Ref.1
	Sequence conflict	275	1	Y → S in AAA26882. Ref.3
	Sequence conflict	367	1	S → R in AAA26882. Ref.3
	Sequence conflict	544	1	T → A in AAC34740. Ref.1
	Sequence conflict	572	1	I → V in AAC34740. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P35596-1 [UniParc]. Last modified September 26, 2001. Version 2. Checksum: A29B42FF4A75E406	FASTA	608	66,796
	10 20 30 40 50 60 MEFSKKTREL SIKKMQERTL DLLIIGGGIT GAGVALQAAA SGLETGLIEM QDFAEGTSSR 70 80 90 100 110 120 STKLVHGGLR YLKQFDVEVV SDTVSERAVV QQIAPHIPKS DPMLLPVYDE DGATFSLFRL 130 140 150 160 170 180 KVAMDLYDLL AGVSNTPAAN KVLSKDQVLE RQPNLKKEGL VGGGVYLDFR NNDARLVIEN 190 200 210 220 230 240 IKRANQDGAL IANHVKAEGF LFDESGKITG VVARDLLTDQ VFEIKARLVI NTTGPWSDKV 250 260 270 280 290 300 RNLSNKGTQF SQMRPTKGVH LVVDSSKIKV SQPVYFDTGL GDGRMVFVLP RENKTYFGTT 310 320 330 340 350 360 DTDYTGDLEH PKVTQEDVDY LLGIVNNRFP ESNITIDDIE SSWAGLRPLI AGNSASDYNG 370 380 390 400 410 420 GNNGTISDES FDNLIATVES YLSKEKTRED VESAVSKLES STSEKHLDPS AVSRGSSLDR 430 440 450 460 470 480 DDNGLLTLAG GKITDYRKMA EGAMERVVDI LKAEFDRSFK LINSKTYPVS GGELNPANVD 490 500 510 520 530 540 SEIEAFAQLG VSRGLDSKEA HYLANLYGSN APKVFALAHS LEQAPGLSLA DTLSLHYAMR 550 560 570 580 590 600 NELTLSPVDF LLRRTNHMLF MRDSLDSIVE PILDEMGRFY DWTEEEKATY RADVEAALAN NDLAELKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The soluble alpha-glycerophosphate oxidase from Enterococcus casseliflavus. Sequence homology with the membrane-associated dehydrogenase and kinetic analysis of the recombinant enzyme." Parsonage D., Luba J., Mallett T.C., Claiborne A. J. Biol. Chem. 273:23812-23822(1998) [PubMed: 9726992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 27336.
[2]	"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M. Science 293:498-506(2001) [PubMed: 11463916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.
[3]	"Genetic identification of exported proteins in Streptococcus pneumoniae." Pearce B.J., Yin Y.B., Masure H.R. Mol. Microbiol. 9:1037-1050(1993) [PubMed: 7934910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 271-408. Strain: R6x.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U94770 Genomic DNA. Translation: AAC34740.1. AE005672 Genomic DNA. Translation: AAK76236.1. L20560 Genomic DNA. Translation: AAA26882.1.
PIR	C95255.
RefSeq	NP_346596.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	930187.
GenomeReviews	Gene locus SP_2185 in contig AE005672_GR.
KEGG	spn:SP_2185.
TIGR	SP_2185.
Phylogenomic databases
HOGENOM	P35596.
OMA	P35596. KDIESSW.
Enzyme and pathway databases
BRENDA	1.1.3.21. 600.
Family and domain databases
InterPro	IPR000447. FAD-dep_Gly3P_DH. IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PRINTS	PR01001. FADG3PDH.
PROSITE	PS00977. FAD_G3PDH_1. 1 hit. PS00978. FAD_G3PDH_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPO_STRPN

Accession

Primary (citable) accession number: P35596
Secondary accession number(s): O87017

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	September 26, 2001
Last modified:	June 16, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)