PTS system glucose-specific EIICBA component

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Reviewed, UniProtKB/Swiss-Prot P35595 (PTG3C_STRPN)

Last modified February 9, 2010. Version 87. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    PTS system glucose-specific EIICBA component
Alternative name(s):
    EIICBA-Glc
    EII-Glc/EIII-Glc
Including the following 3 domains:
    1- Recommended name:
            Glucose permease IIC component
        Alternative name(s):
            PTS system glucose-specific EIIC component
    2- Recommended name:
            Glucose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system glucose-specific EIIB component
    3- Recommended name:
            Glucose-specific phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            PTS system glucose-specific EIIA component

Gene names

Name:	exp5
Ordered Locus Names:	SP_0758

Organism

Streptococcus pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

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Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain. Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

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Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 726

726

PTS system glucose-specific EIICBA component

PRO_0000186561

Regions

Transmembrane

18 – 38

Potential

Transmembrane

62 – 82

Potential

Transmembrane

90 – 110

Potential

Transmembrane

139 – 159

Potential

Transmembrane

184 – 204

Potential

Transmembrane

311 – 331

Potential

Transmembrane

344 – 364

Potential

Transmembrane

365 – 385

Potential

Transmembrane

419 – 439

Potential

Domain

1 – 453

453

PTS EIIC type-1

Domain

473 – 555

PTS EIIB type-1

Domain

596 – 700

105

PTS EIIA type-1

Sites

Active site

495

Phosphocysteine intermediate; for EIIB activity By similarity

Active site

648

Tele-phosphohistidine intermediate; for EIIA activity By similarity

Experimental info

Sequence conflict

471 – 472

AA → DD in AAA26881. Ref.2

Sequence conflict

566

A → V in AAA26881. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

P35595-1 [UniParc].

Last modified September 26, 2001. Version 2.
Checksum: 7C1F7D55ADE16E81
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FASTA

726

77,995

        10         20         30         40         50         60 
MMKDTFKNVL SFEFWQKFGK ALMVVIAVMP AAGLMISIGK SIVMINPTFA PLVITGGILE 

        70         80         90        100        110        120 
QIGWGVIGNL HILFALAIGG SWAKERAGGA FAAGLAFILI NRITGTIFGV SGDMLKNPDA 

       130        140        150        160        170        180 
MVTTFFGGSI KVADYFISVL EAPALNMGVF VGIISGFVGA TAYNKYYNFR KLPDALSFFN 

       190        200        210        220        230        240 
GKRFVPFVVI LRSAIAAILL AAFWPVVQTG INNFGIWIAN SQETAPILAP FLYGTLERLL 

       250        260        270        280        290        300 
LPFGLHHMLT IPMNYTALGG TYDILTGAAK GTQVFGQDPL WLAWVTDLVN LKGTDASQYQ 

       310        320        330        340        350        360 
HLLDTVHPAR FKVGQMIGSF GILMGVIVAI YRNVDADKKH KYKGMMIATA LATFLTGVTE 

       370        380        390        400        410        420 
PIEYMFMFIA TPMYLVYSLV QGAAFAMADV VNLRMHSFGS IEFLTRTPIA ISAGIGMDIV 

       430        440        450        460        470        480 
NFVWVTVLFA VIMYFIANFM IQKFNYATPG RNGNYETAEG SEETSSEVKV AAGSQAVNII 

       490        500        510        520        530        540 
NLLGGRVNIV DVDACMTRLR VTVKDADKVG NAEQWKAEGA MGLVMKGQGV QAIYGPKADI 

       550        560        570        580        590        600 
LKSDIQDILD SGEIIPETLP SQMTEAQQNT VHFKDLTEEV YSVADGQVVA LEQVKDPVFA 

       610        620        630        640        650        660 
QKMMGDGFAV EPANGNIVSP VSGTVSSIFP TKHAFGIVTE AGLEVLVHIG LDTVSLEGKP 

       670        680        690        700        710        720 
FTVHVAEGQK VAAGDLLVTA DLDAIRAAGR ETSTVVVFTN GDAIKSVKLE KTGSLAAKTA 


VAKVEL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. Fraser C.M. Science 293:498-506(2001) [PubMed: 11463916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.
[2]	"Genetic identification of exported proteins in Streptococcus pneumoniae." Pearce B.J., Yin Y.B., Masure H.R. Mol. Microbiol. 9:1037-1050(1993) [PubMed: 7934910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 468-575. Strain: R6x.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005672 Genomic DNA. Translation: AAK74896.1. L20559 Genomic DNA. Translation: AAA26881.1.
PIR	G95087.
RefSeq	NP_345256.1.
3D structure databases
SMR	P35595. Positions 476-552, 573-724.
ModBase	Search...
Genome annotation databases
GeneID	930708.
GenomeReviews	Gene locus SP_0758 in contig AE005672_GR.
KEGG	spn:SP_0758.
TIGR	SP_0758.
Phylogenomic databases
HOGENOM	HBG571563.
OMA	ADVDACM.
Enzyme and pathway databases
BRENDA	2.7.1.69. 600.
Family and domain databases
InterPro	IPR011055. Dup_hybrid_motif. IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR001127. PTS_EIIA_1_perm. IPR001996. PTS_EIIB_1. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. IPR011535. PTS_Glc-like_IIB_component. IPR011300. PTS_IIBC. [Graphical view]
Gene3D	G3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
Pfam	PF00358. PTS_EIIA_1. 1 hit. PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
TIGRFAMs	TIGR00826. EIIB_glc. 1 hit. TIGR00830. PTBA. 1 hit. TIGR02003. PTS-II-BC-unk1. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit. PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

PTG3C_STRPN

Accession

Primary (citable) accession number: P35595

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	September 26, 2001
Last modified:	February 9, 2010
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents