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Protein

PTS system glucose-specific EIICBA component

Gene

exp5

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) = [protein]-L-histidine + D-glucose 6-phosphate(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei495 – 4951Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation
Active sitei648 – 6481Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-760-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system glucose-specific EIICBA component
Alternative name(s):
EII-Glc/EIII-Glc
EIICBA-Glc
Including the following 3 domains:
Glucose permease IIC component
Alternative name(s):
PTS system glucose-specific EIIC component
Glucose-specific phosphotransferase enzyme IIB component (EC:2.7.1.199)
Alternative name(s):
PTS system glucose-specific EIIB component
Glucose-specific phosphotransferase enzyme IIA component
Alternative name(s):
PTS system glucose-specific EIIA component
Gene namesi
Name:exp5
Ordered Locus Names:SP_0758
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

Subcellular locationi

  • Cell membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei18 – 3821HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei62 – 8221HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei90 – 11021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei139 – 15921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei184 – 20421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei311 – 33121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei344 – 36421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei365 – 38521HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei419 – 43921HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 726726PTS system glucose-specific EIICBA componentPRO_0000186561Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi170187.SpneT_02000490.

Structurei

3D structure databases

ProteinModelPortaliP35595.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 453453PTS EIIC type-1PROSITE-ProRule annotationAdd
BLAST
Domaini473 – 55583PTS EIIB type-1PROSITE-ProRule annotationAdd
BLAST
Domaini596 – 700105PTS EIIA type-1PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Sequence similaritiesi

Contains 1 PTS EIIA type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIB type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CI1. Bacteria.
COG1263. LUCA.
COG1264. LUCA.
COG2190. LUCA.
HOGENOMiHOG000250993.
KOiK02777.
K02778.
K02779.
OMAiEFMFMFV.
OrthoDBiEOG6FFS9V.

Family and domain databases

Gene3Di3.30.1360.60. 1 hit.
InterProiIPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB_Cys.
IPR001127. PTS_EIIA_1_perm.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011300. PTS_IIBC.
[Graphical view]
PfamiPF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
TIGR02003. PTS-II-BC-unk1. 1 hit.
PROSITEiPS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35595-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKDTFKNVL SFEFWQKFGK ALMVVIAVMP AAGLMISIGK SIVMINPTFA
60 70 80 90 100
PLVITGGILE QIGWGVIGNL HILFALAIGG SWAKERAGGA FAAGLAFILI
110 120 130 140 150
NRITGTIFGV SGDMLKNPDA MVTTFFGGSI KVADYFISVL EAPALNMGVF
160 170 180 190 200
VGIISGFVGA TAYNKYYNFR KLPDALSFFN GKRFVPFVVI LRSAIAAILL
210 220 230 240 250
AAFWPVVQTG INNFGIWIAN SQETAPILAP FLYGTLERLL LPFGLHHMLT
260 270 280 290 300
IPMNYTALGG TYDILTGAAK GTQVFGQDPL WLAWVTDLVN LKGTDASQYQ
310 320 330 340 350
HLLDTVHPAR FKVGQMIGSF GILMGVIVAI YRNVDADKKH KYKGMMIATA
360 370 380 390 400
LATFLTGVTE PIEYMFMFIA TPMYLVYSLV QGAAFAMADV VNLRMHSFGS
410 420 430 440 450
IEFLTRTPIA ISAGIGMDIV NFVWVTVLFA VIMYFIANFM IQKFNYATPG
460 470 480 490 500
RNGNYETAEG SEETSSEVKV AAGSQAVNII NLLGGRVNIV DVDACMTRLR
510 520 530 540 550
VTVKDADKVG NAEQWKAEGA MGLVMKGQGV QAIYGPKADI LKSDIQDILD
560 570 580 590 600
SGEIIPETLP SQMTEAQQNT VHFKDLTEEV YSVADGQVVA LEQVKDPVFA
610 620 630 640 650
QKMMGDGFAV EPANGNIVSP VSGTVSSIFP TKHAFGIVTE AGLEVLVHIG
660 670 680 690 700
LDTVSLEGKP FTVHVAEGQK VAAGDLLVTA DLDAIRAAGR ETSTVVVFTN
710 720
GDAIKSVKLE KTGSLAAKTA VAKVEL
Length:726
Mass (Da):77,995
Last modified:September 26, 2001 - v2
Checksum:i7C1F7D55ADE16E81
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti471 – 4722AA → DD in AAA26881 (PubMed:7934910).Curated
Sequence conflicti566 – 5661A → V in AAA26881 (PubMed:7934910).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK74896.1.
L20559 Genomic DNA. Translation: AAA26881.1.
PIRiG95087.

Genome annotation databases

EnsemblBacteriaiAAK74896; AAK74896; SP_0758.
KEGGispn:SP_0758.
PATRICi19705883. VBIStrPne105772_0788.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK74896.1.
L20559 Genomic DNA. Translation: AAA26881.1.
PIRiG95087.

3D structure databases

ProteinModelPortaliP35595.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi170187.SpneT_02000490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK74896; AAK74896; SP_0758.
KEGGispn:SP_0758.
PATRICi19705883. VBIStrPne105772_0788.

Phylogenomic databases

eggNOGiENOG4105CI1. Bacteria.
COG1263. LUCA.
COG1264. LUCA.
COG2190. LUCA.
HOGENOMiHOG000250993.
KOiK02777.
K02778.
K02779.
OMAiEFMFMFV.
OrthoDBiEOG6FFS9V.

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-760-MONOMER.

Family and domain databases

Gene3Di3.30.1360.60. 1 hit.
InterProiIPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB_Cys.
IPR001127. PTS_EIIA_1_perm.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011300. PTS_IIBC.
[Graphical view]
PfamiPF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
TIGR02003. PTS-II-BC-unk1. 1 hit.
PROSITEiPS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-334 / TIGR4.
  2. "Genetic identification of exported proteins in Streptococcus pneumoniae."
    Pearce B.J., Yin Y.B., Masure H.R.
    Mol. Microbiol. 9:1037-1050(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 468-575.
    Strain: R6x.

Entry informationi

Entry nameiPTG3C_STRPN
AccessioniPrimary (citable) accession number: P35595
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: September 26, 2001
Last modified: May 11, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.