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Protein

Tyrosine-protein kinase receptor Tie-1

Gene

TIE1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei870ATPPROSITE-ProRule annotation1
Active sitei979Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi845 – 853ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • transmembrane receptor protein tyrosine kinase activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Angiogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS00865-MONOMER.
BRENDAi2.7.10.1. 2681.
SignaLinkiP35590.
SIGNORiP35590.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase receptor Tie-1 (EC:2.7.10.1)
Gene namesi
Name:TIE1
Synonyms:TIE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:11809. TIE1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 759ExtracellularSequence analysisAdd BLAST738
Transmembranei760 – 784HelicalSequence analysisAdd BLAST25
Topological domaini785 – 1138CytoplasmicSequence analysisAdd BLAST354

GO - Cellular componenti

  • integral component of plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi7075.
OpenTargetsiENSG00000066056.
PharmGKBiPA36516.

Chemistry databases

ChEMBLiCHEMBL5274.
GuidetoPHARMACOLOGYi1841.

Polymorphism and mutation databases

BioMutaiTIE1.
DMDMi549081.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000002447122 – 1138Tyrosine-protein kinase receptor Tie-1Add BLAST1117

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi83N-linked (GlcNAc...)Sequence analysis1
Glycosylationi161N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi228 ↔ 237PROSITE-ProRule annotation
Disulfide bondi231 ↔ 244PROSITE-ProRule annotation
Disulfide bondi246 ↔ 255PROSITE-ProRule annotation
Disulfide bondi315 ↔ 327PROSITE-ProRule annotation
Disulfide bondi321 ↔ 333PROSITE-ProRule annotation
Disulfide bondi335 ↔ 344PROSITE-ProRule annotation
Glycosylationi503N-linked (GlcNAc...)Sequence analysis1
Glycosylationi596N-linked (GlcNAc...)Sequence analysis1
Glycosylationi709N-linked (GlcNAc...)Sequence analysis1
Modified residuei1007Phosphotyrosine; by autocatalysisBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP35590.
PeptideAtlasiP35590.
PRIDEiP35590.

PTM databases

iPTMnetiP35590.
PhosphoSitePlusiP35590.

Expressioni

Tissue specificityi

Specifically expressed in developing vascular endothelial cells.

Gene expression databases

BgeeiENSG00000066056.
CleanExiHS_TIE1.
GenevisibleiP35590. HS.

Interactioni

Subunit structurei

Heterodimer with TEK/TIE2.

Binary interactionsi

WithEntry#Exp.IntActNotes
KRT40Q6A1623EBI-2256865,EBI-10171697
PSG5Q152383EBI-2256865,EBI-4314891
RBPMSQ930623EBI-2256865,EBI-740322

Protein-protein interaction databases

BioGridi112931. 9 interactors.
IntActiP35590. 13 interactors.
MINTiMINT-6773015.
STRINGi9606.ENSP00000361554.

Chemistry databases

BindingDBiP35590.

Structurei

3D structure databases

ProteinModelPortaliP35590.
SMRiP35590.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 105Ig-like C2-type 1Add BLAST63
Domaini214 – 256EGF-like 1PROSITE-ProRule annotationAdd BLAST43
Domaini258 – 303EGF-like 2PROSITE-ProRule annotationAdd BLAST46
Domaini305 – 345EGF-like 3PROSITE-ProRule annotationAdd BLAST41
Domaini372 – 426Ig-like C2-type 2Add BLAST55
Domaini446 – 545Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST100
Domaini548 – 642Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST95
Domaini646 – 739Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST94
Domaini839 – 1118Protein kinasePROSITE-ProRule annotationAdd BLAST280

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.PROSITE-ProRule annotation
Contains 3 EGF-like domains.PROSITE-ProRule annotation
Contains 3 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0200. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00810000125384.
HOGENOMiHOG000049232.
HOVERGENiHBG007316.
InParanoidiP35590.
KOiK05120.
OMAiHNSPGAH.
OrthoDBiEOG091G00RL.
PhylomeDBiP35590.
TreeFamiTF317568.

Family and domain databases

CDDicd00063. FN3. 3 hits.
Gene3Di2.60.40.10. 5 hits.
InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00041. fn3. 3 hits.
PF00047. ig. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00181. EGF. 3 hits.
SM00060. FN3. 3 hits.
SM00409. IG. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF49265. SSF49265. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 3 hits.
PS50026. EGF_3. 2 hits.
PS50853. FN3. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P35590-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVWRVPPFLL PILFLASHVG AAVDLTLLAN LRLTDPQRFF LTCVSGEAGA
60 70 80 90 100
GRGSDAWGPP LLLEKDDRIV RTPPGPPLRL ARNGSHQVTL RGFSKPSDLV
110 120 130 140 150
GVFSCVGGAG ARRTRVIYVH NSPGAHLLPD KVTHTVNKGD TAVLSARVHK
160 170 180 190 200
EKQTDVIWKS NGSYFYTLDW HEAQDGRFLL QLPNVQPPSS GIYSATYLEA
210 220 230 240 250
SPLGSAFFRL IVRGCGAGRW GPGCTKECPG CLHGGVCHDH DGECVCPPGF
260 270 280 290 300
TGTRCEQACR EGRFGQSCQE QCPGISGCRG LTFCLPDPYG CSCGSGWRGS
310 320 330 340 350
QCQEACAPGH FGADCRLQCQ CQNGGTCDRF SGCVCPSGWH GVHCEKSDRI
360 370 380 390 400
PQILNMASEL EFNLETMPRI NCAAAGNPFP VRGSIELRKP DGTVLLSTKA
410 420 430 440 450
IVEPEKTTAE FEVPRLVLAD SGFWECRVST SGGQDSRRFK VNVKVPPVPL
460 470 480 490 500
AAPRLLTKQS RQLVVSPLVS FSGDGPISTV RLHYRPQDST MDWSTIVVDP
510 520 530 540 550
SENVTLMNLR PKTGYSVRVQ LSRPGEGGEG AWGPPTLMTT DCPEPLLQPW
560 570 580 590 600
LEGWHVEGTD RLRVSWSLPL VPGPLVGDGF LLRLWDGTRG QERRENVSSP
610 620 630 640 650
QARTALLTGL TPGTHYQLDV QLYHCTLLGP ASPPAHVLLP PSGPPAPRHL
660 670 680 690 700
HAQALSDSEI QLTWKHPEAL PGPISKYVVE VQVAGGAGDP LWIDVDRPEE
710 720 730 740 750
TSTIIRGLNA STRYLFRMRA SIQGLGDWSN TVEESTLGNG LQAEGPVQES
760 770 780 790 800
RAAEEGLDQQ LILAVVGSVS ATCLTILAAL LTLVCIRRSC LHRRRTFTYQ
810 820 830 840 850
SGSGEETILQ FSSGTLTLTR RPKLQPEPLS YPVLEWEDIT FEDLIGEGNF
860 870 880 890 900
GQVIRAMIKK DGLKMNAAIK MLKEYASEND HRDFAGELEV LCKLGHHPNI
910 920 930 940 950
INLLGACKNR GYLYIAIEYA PYGNLLDFLR KSRVLETDPA FAREHGTAST
960 970 980 990 1000
LSSRQLLRFA SDAANGMQYL SEKQFIHRDL AARNVLVGEN LASKIADFGL
1010 1020 1030 1040 1050
SRGEEVYVKK TMGRLPVRWM AIESLNYSVY TTKSDVWSFG VLLWEIVSLG
1060 1070 1080 1090 1100
GTPYCGMTCA ELYEKLPQGY RMEQPRNCDD EVYELMRQCW RDRPYERPPF
1110 1120 1130
AQIALQLGRM LEARKAYVNM SLFENFTYAG IDATAEEA
Length:1,138
Mass (Da):125,090
Last modified:June 1, 1994 - v1
Checksum:i3B42BE33678C58A1
GO
Isoform 2 (identifier: P35590-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     348-379: DRIPQILNMASELEFNLETMPRINCAAAGNPF → GWRDWVDTSTEKQNTDEGRFGGHVSAPVGAPG
     380-1138: Missing.

Show »
Length:379
Mass (Da):40,616
Checksum:i8EACCD600795FF09
GO
Isoform 3 (identifier: P35590-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     305-317: ACAPGHFGADCRL → VHQGHCGAREDHS
     318-1138: Missing.

Show »
Length:317
Mass (Da):34,138
Checksum:i456907881A613CE4
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_041852448V → M.1 PublicationCorresponds to variant rs56302794dbSNPEnsembl.1
Natural variantiVAR_0418531104A → V.1 PublicationCorresponds to variant rs35573981dbSNPEnsembl.1
Natural variantiVAR_0418541109R → H.1 PublicationCorresponds to variant rs34993202dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047607305 – 317ACAPG…ADCRL → VHQGHCGAREDHS in isoform 3. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_047608318 – 1138Missing in isoform 3. 1 PublicationAdd BLAST821
Alternative sequenceiVSP_047609348 – 379DRIPQ…AGNPF → GWRDWVDTSTEKQNTDEGRF GGHVSAPVGAPG in isoform 2. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_047610380 – 1138Missing in isoform 2. 1 PublicationAdd BLAST759

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60957 mRNA. Translation: CAA43290.1.
EU826587 mRNA. Translation: ACF47623.1.
EU826588 mRNA. Translation: ACF47624.1.
AC093420 Genomic DNA. No translation available.
AL139289 Genomic DNA. No translation available.
BC038239 mRNA. Translation: AAH38239.1.
CCDSiCCDS482.1. [P35590-1]
RefSeqiNP_005415.1. NM_005424.4. [P35590-1]
UniGeneiHs.78824.

Genome annotation databases

EnsembliENST00000372476; ENSP00000361554; ENSG00000066056. [P35590-1]
ENST00000538015; ENSP00000440063; ENSG00000066056. [P35590-2]
GeneIDi7075.
KEGGihsa:7075.
UCSCiuc001ciu.4. human. [P35590-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60957 mRNA. Translation: CAA43290.1.
EU826587 mRNA. Translation: ACF47623.1.
EU826588 mRNA. Translation: ACF47624.1.
AC093420 Genomic DNA. No translation available.
AL139289 Genomic DNA. No translation available.
BC038239 mRNA. Translation: AAH38239.1.
CCDSiCCDS482.1. [P35590-1]
RefSeqiNP_005415.1. NM_005424.4. [P35590-1]
UniGeneiHs.78824.

3D structure databases

ProteinModelPortaliP35590.
SMRiP35590.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112931. 9 interactors.
IntActiP35590. 13 interactors.
MINTiMINT-6773015.
STRINGi9606.ENSP00000361554.

Chemistry databases

BindingDBiP35590.
ChEMBLiCHEMBL5274.
GuidetoPHARMACOLOGYi1841.

PTM databases

iPTMnetiP35590.
PhosphoSitePlusiP35590.

Polymorphism and mutation databases

BioMutaiTIE1.
DMDMi549081.

Proteomic databases

PaxDbiP35590.
PeptideAtlasiP35590.
PRIDEiP35590.

Protocols and materials databases

DNASUi7075.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372476; ENSP00000361554; ENSG00000066056. [P35590-1]
ENST00000538015; ENSP00000440063; ENSG00000066056. [P35590-2]
GeneIDi7075.
KEGGihsa:7075.
UCSCiuc001ciu.4. human. [P35590-1]

Organism-specific databases

CTDi7075.
DisGeNETi7075.
GeneCardsiTIE1.
HGNCiHGNC:11809. TIE1.
MIMi600222. gene.
neXtProtiNX_P35590.
OpenTargetsiENSG00000066056.
PharmGKBiPA36516.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0200. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00810000125384.
HOGENOMiHOG000049232.
HOVERGENiHBG007316.
InParanoidiP35590.
KOiK05120.
OMAiHNSPGAH.
OrthoDBiEOG091G00RL.
PhylomeDBiP35590.
TreeFamiTF317568.

Enzyme and pathway databases

BioCyciZFISH:HS00865-MONOMER.
BRENDAi2.7.10.1. 2681.
SignaLinkiP35590.
SIGNORiP35590.

Miscellaneous databases

ChiTaRSiTIE1. human.
GenomeRNAii7075.
PROiP35590.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000066056.
CleanExiHS_TIE1.
GenevisibleiP35590. HS.

Family and domain databases

CDDicd00063. FN3. 3 hits.
Gene3Di2.60.40.10. 5 hits.
InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00041. fn3. 3 hits.
PF00047. ig. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00181. EGF. 3 hits.
SM00060. FN3. 3 hits.
SM00409. IG. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF49265. SSF49265. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 3 hits.
PS50026. EGF_3. 2 hits.
PS50853. FN3. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIE1_HUMAN
AccessioniPrimary (citable) accession number: P35590
Secondary accession number(s): B5A949, B5A950
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 172 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.