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P35585

- AP1M1_MOUSE

UniProt

P35585 - AP1M1_MOUSE

Protein

AP-1 complex subunit mu-1

Gene

Ap1m1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. endosome to melanosome transport Source: Ensembl
    2. intracellular protein transport Source: MGI
    3. melanosome organization Source: Ensembl
    4. vesicle-mediated transport Source: MGI

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_196550. MHC class II antigen presentation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-1 complex subunit mu-1
    Alternative name(s):
    AP-mu chain family member mu1A
    Adaptor protein complex AP-1 subunit mu-1
    Adaptor-related protein complex 1 subunit mu-1
    Clathrin assembly protein complex 1 mu-1 medium chain 1
    Clathrin coat assembly protein AP47
    Clathrin coat-associated protein AP47
    Golgi adaptor HA1/AP1 adaptin mu-1 subunit
    Mu-adaptin 1
    Mu1A-adaptin
    Gene namesi
    Name:Ap1m1
    Synonyms:Cltnm
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:102776. Ap1m1.

    Subcellular locationi

    Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side
    Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

    GO - Cellular componenti

    1. clathrin adaptor complex Source: InterPro
    2. clathrin-coated vesicle membrane Source: UniProtKB-SubCell
    3. trans-Golgi network Source: MGI

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 423422AP-1 complex subunit mu-1PRO_0000193771Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei152 – 1521Phosphothreonine1 Publication
    Modified residuei154 – 1541Phosphothreonine1 Publication

    Post-translational modificationi

    Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP35585.
    PaxDbiP35585.
    PRIDEiP35585.

    PTM databases

    PhosphoSiteiP35585.

    Expressioni

    Gene expression databases

    ArrayExpressiP35585.
    BgeeiP35585.
    GenevestigatoriP35585.

    Interactioni

    Subunit structurei

    Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Interacts with MARCH11 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AP1B1Q105673EBI-1040251,EBI-1171303From a different organism.
    Ap1g1P228925EBI-1040251,EBI-1040262

    Protein-protein interaction databases

    BioGridi198125. 1 interaction.
    DIPiDIP-35323N.
    IntActiP35585. 6 interactions.
    MINTiMINT-216979.
    STRINGi10090.ENSMUSP00000003117.

    Structurei

    Secondary structure

    1
    423
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 96
    Beta strandi15 – 228
    Helixi27 – 326
    Helixi33 – 4311
    Beta strandi48 – 525
    Beta strandi55 – 628
    Beta strandi65 – 739
    Helixi77 – 9418
    Beta strandi95 – 973
    Helixi100 – 1056
    Helixi107 – 11711
    Helixi128 – 1314
    Turni132 – 1343
    Helixi151 – 1544
    Beta strandi155 – 1573
    Beta strandi167 – 18317
    Beta strandi185 – 1873
    Beta strandi189 – 20315
    Beta strandi205 – 2073
    Beta strandi209 – 2146
    Helixi217 – 2226
    Beta strandi234 – 2385
    Helixi244 – 2507
    Beta strandi253 – 2553
    Beta strandi259 – 26911
    Beta strandi276 – 28712
    Beta strandi290 – 29910
    Beta strandi306 – 31510
    Beta strandi321 – 33515
    Turni336 – 3394
    Beta strandi340 – 35112
    Beta strandi353 – 3619
    Beta strandi376 – 38813
    Beta strandi392 – 3987
    Beta strandi405 – 41410
    Beta strandi416 – 4205

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1W63X-ray4.00M/N/O/P/R/V1-423[»]
    4EMZX-ray2.90A/M158-423[»]
    4EN2X-ray2.58A/M158-423[»]
    4HMYX-ray7.00M1-423[»]
    4P6ZX-ray3.00M1-423[»]
    ProteinModelPortaliP35585.
    SMRiP35585. Positions 2-423.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP35585.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini168 – 421254MHDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG315786.
    HOGENOMiHOG000173247.
    HOVERGENiHBG050516.
    InParanoidiP35585.
    KOiK12393.
    OMAiYPWVRYI.
    OrthoDBiEOG783MV6.
    PhylomeDBiP35585.
    TreeFamiTF300393.

    Family and domain databases

    InterProiIPR022775. AP_mu_sigma_su.
    IPR001392. Clathrin_mu.
    IPR008968. Clathrin_mu_C.
    IPR018240. Clathrin_mu_CS.
    IPR011012. Longin-like_dom.
    IPR028565. MHD.
    [Graphical view]
    PfamiPF00928. Adap_comp_sub. 1 hit.
    PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
    PRINTSiPR00314. CLATHRINADPT.
    SUPFAMiSSF49447. SSF49447. 1 hit.
    SSF64356. SSF64356. 1 hit.
    PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
    PS00991. CLAT_ADAPTOR_M_2. 1 hit.
    PS51072. MHD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35585-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSASAVYVLD LKGKVLICRN YRGDVDMSEV EHFMPILMEK EEEGMLSPIL    50
    AHGGVRFMWI KHNNLYLVAT SKKNACVSLV FSFLYKVVQV FSEYFKELEE 100
    ESIRDNFVII YELLDELMDF GYPQTTDSKI LQEYITQEGH KLETGAPRPP 150
    ATVTNAVSWR SEGIKYRKNE VFLDVIEAVN LLVSANGNVL RSEIVGSIKM 200
    RVFLSGMPEL RLGLNDKVLF DNTGRGKSKS VELEDVKFHQ CVRLSRFEND 250
    RTISFIPPDG EFELMSYRLN THVKPLIWIE SVIEKHSHSR IEYMVKAKSQ 300
    FKRRSTANNV EIHIPVPNDA DSPKFKTTVG SVKWVPENSE IVWSVKSFPG 350
    GKEYLMRAHF GLPSVEAEDK EGKPPISVKF EIPYFTTSGI QVRYLKIIEK 400
    SGYQALPWVR YITQNGDYQL RTQ 423
    Length:423
    Mass (Da):48,543
    Last modified:January 23, 2007 - v3
    Checksum:i1213566B3A681B4C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62419 mRNA. Translation: AAA37244.1.
    AF139405
    , AF139394, AF139395, AF139396, AF139397, AF139398, AF139399, AF139400, AF139401, AF139402, AF139403, AF139404 Genomic DNA. Translation: AAF61814.1.
    BC003823 mRNA. Translation: AAH03823.1.
    CCDSiCCDS22411.1.
    PIRiS19693.
    RefSeqiNP_031482.1. NM_007456.4.
    UniGeneiMm.290086.

    Genome annotation databases

    EnsembliENSMUST00000003117; ENSMUSP00000003117; ENSMUSG00000003033.
    GeneIDi11767.
    KEGGimmu:11767.
    UCSCiuc009mfp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62419 mRNA. Translation: AAA37244.1 .
    AF139405
    , AF139394 , AF139395 , AF139396 , AF139397 , AF139398 , AF139399 , AF139400 , AF139401 , AF139402 , AF139403 , AF139404 Genomic DNA. Translation: AAF61814.1 .
    BC003823 mRNA. Translation: AAH03823.1 .
    CCDSi CCDS22411.1.
    PIRi S19693.
    RefSeqi NP_031482.1. NM_007456.4.
    UniGenei Mm.290086.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1W63 X-ray 4.00 M/N/O/P/R/V 1-423 [» ]
    4EMZ X-ray 2.90 A/M 158-423 [» ]
    4EN2 X-ray 2.58 A/M 158-423 [» ]
    4HMY X-ray 7.00 M 1-423 [» ]
    4P6Z X-ray 3.00 M 1-423 [» ]
    ProteinModelPortali P35585.
    SMRi P35585. Positions 2-423.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198125. 1 interaction.
    DIPi DIP-35323N.
    IntActi P35585. 6 interactions.
    MINTi MINT-216979.
    STRINGi 10090.ENSMUSP00000003117.

    PTM databases

    PhosphoSitei P35585.

    Proteomic databases

    MaxQBi P35585.
    PaxDbi P35585.
    PRIDEi P35585.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000003117 ; ENSMUSP00000003117 ; ENSMUSG00000003033 .
    GeneIDi 11767.
    KEGGi mmu:11767.
    UCSCi uc009mfp.1. mouse.

    Organism-specific databases

    CTDi 8907.
    MGIi MGI:102776. Ap1m1.

    Phylogenomic databases

    eggNOGi NOG315786.
    HOGENOMi HOG000173247.
    HOVERGENi HBG050516.
    InParanoidi P35585.
    KOi K12393.
    OMAi YPWVRYI.
    OrthoDBi EOG783MV6.
    PhylomeDBi P35585.
    TreeFami TF300393.

    Enzyme and pathway databases

    Reactomei REACT_196550. MHC class II antigen presentation.

    Miscellaneous databases

    EvolutionaryTracei P35585.
    NextBioi 279533.
    PROi P35585.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P35585.
    Bgeei P35585.
    Genevestigatori P35585.

    Family and domain databases

    InterProi IPR022775. AP_mu_sigma_su.
    IPR001392. Clathrin_mu.
    IPR008968. Clathrin_mu_C.
    IPR018240. Clathrin_mu_CS.
    IPR011012. Longin-like_dom.
    IPR028565. MHD.
    [Graphical view ]
    Pfami PF00928. Adap_comp_sub. 1 hit.
    PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005992. Clathrin_mu. 1 hit.
    PRINTSi PR00314. CLATHRINADPT.
    SUPFAMi SSF49447. SSF49447. 1 hit.
    SSF64356. SSF64356. 1 hit.
    PROSITEi PS00990. CLAT_ADAPTOR_M_1. 1 hit.
    PS00991. CLAT_ADAPTOR_M_2. 1 hit.
    PS51072. MHD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast."
      Nakayama Y., Goebl M., O'Brine Greco B., Lemmon S., Pingchang C.E., Kirchhausen T.
      Eur. J. Biochem. 202:569-574(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Genomic structure and chromosome mapping of the genes encoding clathrin-associated adaptor medium chains mu1A (Ap1m1) and mu1B (Ap1m2)."
      Nakatsu F., Kadohira T., Gilbert D.J., Jenkins N.A., Kakuta H., Copeland N.G., Saito T., Ohno H.
      Cytogenet. Cell Genet. 87:53-58(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary gland.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-152 AND THR-154, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiAP1M1_MOUSE
    AccessioniPrimary (citable) accession number: P35585
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 118 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3