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P35585 (AP1M1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-1 complex subunit mu-1
Alternative name(s):
AP-mu chain family member mu1A
Adapter-related protein complex 1 subunit mu-1
Adaptor protein complex AP-1 subunit mu-1
Clathrin assembly protein complex 1 mu-1 medium chain 1
Clathrin coat assembly protein AP47
Clathrin coat-associated protein AP47
Golgi adaptor HA1/AP1 adaptin mu-1 subunit
Mu-adaptin 1
Mu1A-adaptin
Gene names
Name:Ap1m1
Synonyms:Cltnm
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Subunit structure

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Interacts with MARCH11 By similarity.

Subcellular location

Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Post-translational modification

Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals By similarity.

Sequence similarities

Belongs to the adaptor complexes medium subunit family.

Contains 1 MHD (mu homology) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

AP1B1Q105673EBI-1040251,EBI-1171303From a different organism.
Ap1g1P228925EBI-1040251,EBI-1040262

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 423422AP-1 complex subunit mu-1
PRO_0000193771

Regions

Domain168 – 421254MHD

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue1521Phosphothreonine Ref.4
Modified residue1541Phosphothreonine Ref.4

Secondary structure

................................... 423
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P35585 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 1213566B3A681B4C

FASTA42348,543
        10         20         30         40         50         60 
MSASAVYVLD LKGKVLICRN YRGDVDMSEV EHFMPILMEK EEEGMLSPIL AHGGVRFMWI 

        70         80         90        100        110        120 
KHNNLYLVAT SKKNACVSLV FSFLYKVVQV FSEYFKELEE ESIRDNFVII YELLDELMDF 

       130        140        150        160        170        180 
GYPQTTDSKI LQEYITQEGH KLETGAPRPP ATVTNAVSWR SEGIKYRKNE VFLDVIEAVN 

       190        200        210        220        230        240 
LLVSANGNVL RSEIVGSIKM RVFLSGMPEL RLGLNDKVLF DNTGRGKSKS VELEDVKFHQ 

       250        260        270        280        290        300 
CVRLSRFEND RTISFIPPDG EFELMSYRLN THVKPLIWIE SVIEKHSHSR IEYMVKAKSQ 

       310        320        330        340        350        360 
FKRRSTANNV EIHIPVPNDA DSPKFKTTVG SVKWVPENSE IVWSVKSFPG GKEYLMRAHF 

       370        380        390        400        410        420 
GLPSVEAEDK EGKPPISVKF EIPYFTTSGI QVRYLKIIEK SGYQALPWVR YITQNGDYQL 


RTQ 

« Hide

References

« Hide 'large scale' references
[1]"The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast."
Nakayama Y., Goebl M., O'Brine Greco B., Lemmon S., Pingchang C.E., Kirchhausen T.
Eur. J. Biochem. 202:569-574(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Genomic structure and chromosome mapping of the genes encoding clathrin-associated adaptor medium chains mu1A (Ap1m1) and mu1B (Ap1m2)."
Nakatsu F., Kadohira T., Gilbert D.J., Jenkins N.A., Kakuta H., Copeland N.G., Saito T., Ohno H.
Cytogenet. Cell Genet. 87:53-58(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary gland.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-152 AND THR-154, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M62419 mRNA. Translation: AAA37244.1.
AF139405 expand/collapse EMBL AC list , AF139394, AF139395, AF139396, AF139397, AF139398, AF139399, AF139400, AF139401, AF139402, AF139403, AF139404 Genomic DNA. Translation: AAF61814.1.
BC003823 mRNA. Translation: AAH03823.1.
PIRS19693.
RefSeqNP_031482.1. NM_007456.4.
UniGeneMm.290086.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1W63X-ray4.00M/N/O/P/R/V1-423[»]
4EMZX-ray2.90A/M158-423[»]
4EN2X-ray2.58A/M158-423[»]
4HMYX-ray7.00M1-423[»]
ProteinModelPortalP35585.
SMRP35585. Positions 2-423.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-35323N.
IntActP35585. 6 interactions.
MINTMINT-216979.
STRING10090.ENSMUSP00000003117.

PTM databases

PhosphoSiteP35585.

Proteomic databases

PaxDbP35585.
PRIDEP35585.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000003117; ENSMUSP00000003117; ENSMUSG00000003033.
GeneID11767.
KEGGmmu:11767.
UCSCuc009mfp.1. mouse.

Organism-specific databases

CTD8907.
MGIMGI:102776. Ap1m1.

Phylogenomic databases

eggNOGNOG315786.
HOGENOMHOG000173247.
HOVERGENHBG050516.
InParanoidP35585.
KOK12393.
OMAYPWVRYI.
OrthoDBEOG783MV6.
PhylomeDBP35585.
TreeFamTF300393.

Gene expression databases

ArrayExpressP35585.
BgeeP35585.
GenevestigatorP35585.

Family and domain databases

InterProIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR008968. Clathrin_mu_C.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFPIRSF005992. Clathrin_mu. 1 hit.
PRINTSPR00314. CLATHRINADPT.
SUPFAMSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP35585.
NextBio279533.
PROP35585.
SOURCESearch...

Entry information

Entry nameAP1M1_MOUSE
AccessionPrimary (citable) accession number: P35585
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot