AP-1 complex subunit mu-1 - Mus musculus (Mouse)

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P35585 (AP1M1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
AP-1 complex subunit mu-1

Alternative name(s):
AP-mu chain family member mu1A
Adaptor protein complex AP-1 mu-1 subunit
Adaptor-related protein complex 1 mu-1 subunit
Clathrin assembly protein complex 1 medium chain 1
Clathrin coat assembly protein AP47
Clathrin coat-associated protein AP47
Golgi adaptor HA1/AP1 adaptin mu-1 subunit
Mu-adaptin 1
Mu1A-adaptin

Gene names

Name:	Ap1m1
Synonyms:	Cltnm

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Subunit structure	Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Interacts with MARCH11 By similarity.
Subcellular location	Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Post-translational modification	Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals By similarity.
Sequence similarities	Belongs to the adaptor complexes medium subunit family. Contains 1 MHD (mu homology) domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endosome to melanosome transport Inferred from electronic annotation. Source: Compara intracellular protein transport Traceable author statement PubMed 9714600. Source: MGI melanosome organization Inferred from electronic annotation. Source: Compara vesicle-mediated transport Traceable author statement PubMed 9714600. Source: MGI
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell clathrin adaptor complex Inferred from electronic annotation. Source: InterPro clathrin-coated vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell trans-Golgi network Traceable author statement PubMed 9714600. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 423

422

AP-1 complex subunit mu-1

PRO_0000193771

Regions

Domain

165 – 422

258

MHD

Amino acid modifications

Modified residue

N-acetylserine By similarity

Modified residue

152

Phosphothreonine Ref.4

Modified residue

154

Phosphothreonine Ref.4

Secondary structure

423

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P35585 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 1213566B3A681B4C
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FASTA

423

48,543

        10         20         30         40         50         60 
MSASAVYVLD LKGKVLICRN YRGDVDMSEV EHFMPILMEK EEEGMLSPIL AHGGVRFMWI 

        70         80         90        100        110        120 
KHNNLYLVAT SKKNACVSLV FSFLYKVVQV FSEYFKELEE ESIRDNFVII YELLDELMDF 

       130        140        150        160        170        180 
GYPQTTDSKI LQEYITQEGH KLETGAPRPP ATVTNAVSWR SEGIKYRKNE VFLDVIEAVN 

       190        200        210        220        230        240 
LLVSANGNVL RSEIVGSIKM RVFLSGMPEL RLGLNDKVLF DNTGRGKSKS VELEDVKFHQ 

       250        260        270        280        290        300 
CVRLSRFEND RTISFIPPDG EFELMSYRLN THVKPLIWIE SVIEKHSHSR IEYMVKAKSQ 

       310        320        330        340        350        360 
FKRRSTANNV EIHIPVPNDA DSPKFKTTVG SVKWVPENSE IVWSVKSFPG GKEYLMRAHF 

       370        380        390        400        410        420 
GLPSVEAEDK EGKPPISVKF EIPYFTTSGI QVRYLKIIEK SGYQALPWVR YITQNGDYQL 


RTQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast." Nakayama Y., Goebl M., O'Brine Greco B., Lemmon S., Pingchang C.E., Kirchhausen T. Eur. J. Biochem. 202:569-574(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Genomic structure and chromosome mapping of the genes encoding clathrin-associated adaptor medium chains mu1A (Ap1m1) and mu1B (Ap1m2)." Nakatsu F., Kadohira T., Gilbert D.J., Jenkins N.A., Kakuta H., Copeland N.G., Saito T., Ohno H. Cytogenet. Cell Genet. 87:53-58(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary gland.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-152 AND THR-154, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M62419 mRNA. Translation: AAA37244.1.
AF139405

AF139404 Genomic DNA. Translation: AAF61814.1.
BC003823 mRNA. Translation: AAH03823.1.

IPI

IPI00119680.

PIR

S19693.

RefSeq

NP_031482.1. NM_007456.4.

UniGene

Mm.290086.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1W63	X-ray	4.00	M/N/O/P/R/V	1-423	[»]
4EMZ	X-ray	2.90	A/M	158-423	[»]
4EN2	X-ray	2.58	A/M	158-423	[»]
4HMY	X-ray	7.00	M	1-423	[»]

ProteinModelPortal

P35585.

SMR

P35585. Positions 2-423.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35323N.

IntAct

P35585. 1 interaction.

STRING

10090.ENSMUSP00000003117.

PTM databases

PhosphoSite

P35585.

Proteomic databases

PaxDb

P35585.

PRIDE

P35585.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000003117; ENSMUSP00000003117; ENSMUSG00000003033.

GeneID

11767.

KEGG

mmu:11767.

Organism-specific databases

CTD

8907.

MGI

MGI:102776. Ap1m1.

Phylogenomic databases

eggNOG

NOG315786.

HOGENOM

HOG000173247.

HOVERGEN

HBG050516.

InParanoid

P35585.

K12393.

OMA

IVGSVKM.

OrthoDB

EOG4CJVH3.

Gene expression databases

ArrayExpress

P35585.

Bgee

P35585.

Genevestigator

P35585.

GermOnline

ENSMUSG00000003033. Mus musculus.

Family and domain databases

InterPro

IPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR008968. Clathrin_mu_C.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
[Graphical view]

Pfam

PF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]

PIRSF

PIRSF005992. Clathrin_mu. 1 hit.

PRINTS

PR00314. CLATHRINADPT.

SUPFAM

SSF49447. AP50. 1 hit.
SSF64356. Longin_like. 1 hit.

PROSITE

PS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P35585.

NextBio

279533.

SOURCE

Search...

Entry information

Entry name

AP1M1_MOUSE

Accession

Primary (citable) accession number: P35585

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 105 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents