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Protein

AP-1 complex subunit mu-1

Gene

Ap1m1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

GO - Biological processi

  • endosome to melanosome transport Source: MGI
  • intracellular protein transport Source: MGI
  • melanosome organization Source: MGI
  • vesicle-mediated transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-432722. Golgi Associated Vesicle Biogenesis.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit mu-1
Alternative name(s):
AP-mu chain family member mu1A
Adaptor protein complex AP-1 subunit mu-1
Adaptor-related protein complex 1 subunit mu-1
Clathrin assembly protein complex 1 mu-1 medium chain 1
Clathrin coat assembly protein AP47
Clathrin coat-associated protein AP47
Golgi adaptor HA1/AP1 adaptin mu-1 subunit
Mu-adaptin 1
Mu1A-adaptin
Gene namesi
Name:Ap1m1
Synonyms:Cltnm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:102776. Ap1m1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001937712 – 423AP-1 complex subunit mu-1Add BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei152PhosphothreonineCombined sources1
Modified residuei154PhosphothreonineCombined sources1
Modified residuei223PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP35585.
PaxDbiP35585.
PeptideAtlasiP35585.
PRIDEiP35585.

PTM databases

iPTMnetiP35585.
PhosphoSitePlusiP35585.

Expressioni

Gene expression databases

BgeeiENSMUSG00000003033.
ExpressionAtlasiP35585. baseline and differential.
GenevisibleiP35585. MM.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Interacts with MARCH11 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
AP1B1Q105675EBI-1040251,EBI-1171303From a different organism.
Ap1g1P228926EBI-1040251,EBI-1040262
BST2Q105892EBI-1040251,EBI-2476339From a different organism.

Protein-protein interaction databases

BioGridi198125. 1 interactor.
DIPiDIP-35323N.
IntActiP35585. 9 interactors.
MINTiMINT-216979.
STRINGi10090.ENSMUSP00000003117.

Structurei

Secondary structure

1423
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Beta strandi15 – 22Combined sources8
Helixi27 – 32Combined sources6
Helixi33 – 43Combined sources11
Beta strandi48 – 52Combined sources5
Beta strandi55 – 62Combined sources8
Beta strandi65 – 73Combined sources9
Helixi77 – 94Combined sources18
Beta strandi95 – 97Combined sources3
Helixi100 – 105Combined sources6
Helixi107 – 117Combined sources11
Helixi128 – 131Combined sources4
Turni132 – 134Combined sources3
Helixi151 – 154Combined sources4
Beta strandi155 – 157Combined sources3
Beta strandi167 – 183Combined sources17
Beta strandi185 – 187Combined sources3
Beta strandi189 – 203Combined sources15
Beta strandi205 – 207Combined sources3
Beta strandi209 – 214Combined sources6
Helixi217 – 222Combined sources6
Beta strandi234 – 238Combined sources5
Helixi244 – 250Combined sources7
Beta strandi253 – 255Combined sources3
Beta strandi259 – 269Combined sources11
Beta strandi276 – 287Combined sources12
Beta strandi290 – 299Combined sources10
Beta strandi306 – 315Combined sources10
Beta strandi321 – 335Combined sources15
Turni336 – 339Combined sources4
Beta strandi340 – 351Combined sources12
Beta strandi353 – 361Combined sources9
Beta strandi376 – 388Combined sources13
Beta strandi392 – 398Combined sources7
Beta strandi405 – 414Combined sources10
Beta strandi416 – 420Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1W63X-ray4.00M/N/O/P/R/V1-423[»]
4EMZX-ray2.90A/M158-423[»]
4EN2X-ray2.58A/M158-423[»]
4HMYX-ray7.00M1-423[»]
4P6ZX-ray3.00M1-423[»]
ProteinModelPortaliP35585.
SMRiP35585.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35585.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini168 – 421MHDPROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0937. Eukaryota.
ENOG410XPFS. LUCA.
GeneTreeiENSGT00530000062779.
HOGENOMiHOG000173247.
HOVERGENiHBG050516.
InParanoidiP35585.
KOiK12393.
OMAiITQNGEY.
OrthoDBiEOG091G06XI.
PhylomeDBiP35585.
TreeFamiTF300393.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35585-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASAVYVLD LKGKVLICRN YRGDVDMSEV EHFMPILMEK EEEGMLSPIL
60 70 80 90 100
AHGGVRFMWI KHNNLYLVAT SKKNACVSLV FSFLYKVVQV FSEYFKELEE
110 120 130 140 150
ESIRDNFVII YELLDELMDF GYPQTTDSKI LQEYITQEGH KLETGAPRPP
160 170 180 190 200
ATVTNAVSWR SEGIKYRKNE VFLDVIEAVN LLVSANGNVL RSEIVGSIKM
210 220 230 240 250
RVFLSGMPEL RLGLNDKVLF DNTGRGKSKS VELEDVKFHQ CVRLSRFEND
260 270 280 290 300
RTISFIPPDG EFELMSYRLN THVKPLIWIE SVIEKHSHSR IEYMVKAKSQ
310 320 330 340 350
FKRRSTANNV EIHIPVPNDA DSPKFKTTVG SVKWVPENSE IVWSVKSFPG
360 370 380 390 400
GKEYLMRAHF GLPSVEAEDK EGKPPISVKF EIPYFTTSGI QVRYLKIIEK
410 420
SGYQALPWVR YITQNGDYQL RTQ
Length:423
Mass (Da):48,543
Last modified:January 23, 2007 - v3
Checksum:i1213566B3A681B4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62419 mRNA. Translation: AAA37244.1.
AF139405
, AF139394, AF139395, AF139396, AF139397, AF139398, AF139399, AF139400, AF139401, AF139402, AF139403, AF139404 Genomic DNA. Translation: AAF61814.1.
BC003823 mRNA. Translation: AAH03823.1.
CCDSiCCDS22411.1.
PIRiS19693.
RefSeqiNP_031482.1. NM_007456.5.
UniGeneiMm.290086.

Genome annotation databases

EnsembliENSMUST00000003117; ENSMUSP00000003117; ENSMUSG00000003033.
GeneIDi11767.
KEGGimmu:11767.
UCSCiuc009mfp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62419 mRNA. Translation: AAA37244.1.
AF139405
, AF139394, AF139395, AF139396, AF139397, AF139398, AF139399, AF139400, AF139401, AF139402, AF139403, AF139404 Genomic DNA. Translation: AAF61814.1.
BC003823 mRNA. Translation: AAH03823.1.
CCDSiCCDS22411.1.
PIRiS19693.
RefSeqiNP_031482.1. NM_007456.5.
UniGeneiMm.290086.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1W63X-ray4.00M/N/O/P/R/V1-423[»]
4EMZX-ray2.90A/M158-423[»]
4EN2X-ray2.58A/M158-423[»]
4HMYX-ray7.00M1-423[»]
4P6ZX-ray3.00M1-423[»]
ProteinModelPortaliP35585.
SMRiP35585.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198125. 1 interactor.
DIPiDIP-35323N.
IntActiP35585. 9 interactors.
MINTiMINT-216979.
STRINGi10090.ENSMUSP00000003117.

PTM databases

iPTMnetiP35585.
PhosphoSitePlusiP35585.

Proteomic databases

EPDiP35585.
PaxDbiP35585.
PeptideAtlasiP35585.
PRIDEiP35585.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003117; ENSMUSP00000003117; ENSMUSG00000003033.
GeneIDi11767.
KEGGimmu:11767.
UCSCiuc009mfp.1. mouse.

Organism-specific databases

CTDi8907.
MGIiMGI:102776. Ap1m1.

Phylogenomic databases

eggNOGiKOG0937. Eukaryota.
ENOG410XPFS. LUCA.
GeneTreeiENSGT00530000062779.
HOGENOMiHOG000173247.
HOVERGENiHBG050516.
InParanoidiP35585.
KOiK12393.
OMAiITQNGEY.
OrthoDBiEOG091G06XI.
PhylomeDBiP35585.
TreeFamiTF300393.

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-432722. Golgi Associated Vesicle Biogenesis.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiP35585.
PROiP35585.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003033.
ExpressionAtlasiP35585. baseline and differential.
GenevisibleiP35585. MM.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAP1M1_MOUSE
AccessioniPrimary (citable) accession number: P35585
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.