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P35585

- AP1M1_MOUSE

UniProt

P35585 - AP1M1_MOUSE

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Protein
AP-1 complex subunit mu-1
Gene
Ap1m1, Cltnm
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. endosome to melanosome transport Source: Ensembl
  2. intracellular protein transport Source: MGI
  3. melanosome organization Source: Ensembl
  4. vesicle-mediated transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_196550. MHC class II antigen presentation.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit mu-1
Alternative name(s):
AP-mu chain family member mu1A
Adaptor protein complex AP-1 subunit mu-1
Adaptor-related protein complex 1 subunit mu-1
Clathrin assembly protein complex 1 mu-1 medium chain 1
Clathrin coat assembly protein AP47
Clathrin coat-associated protein AP47
Golgi adaptor HA1/AP1 adaptin mu-1 subunit
Mu-adaptin 1
Mu1A-adaptin
Gene namesi
Name:Ap1m1
Synonyms:Cltnm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:102776. Ap1m1.

Subcellular locationi

Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

GO - Cellular componenti

  1. clathrin adaptor complex Source: InterPro
  2. clathrin-coated vesicle membrane Source: UniProtKB-SubCell
  3. trans-Golgi network Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 423422AP-1 complex subunit mu-1
PRO_0000193771Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei152 – 1521Phosphothreonine1 Publication
Modified residuei154 – 1541Phosphothreonine1 Publication

Post-translational modificationi

Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP35585.
PaxDbiP35585.
PRIDEiP35585.

PTM databases

PhosphoSiteiP35585.

Expressioni

Gene expression databases

ArrayExpressiP35585.
BgeeiP35585.
GenevestigatoriP35585.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Interacts with MARCH11 By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
AP1B1Q105673EBI-1040251,EBI-1171303From a different organism.
Ap1g1P228925EBI-1040251,EBI-1040262

Protein-protein interaction databases

BioGridi198125. 1 interaction.
DIPiDIP-35323N.
IntActiP35585. 6 interactions.
MINTiMINT-216979.
STRINGi10090.ENSMUSP00000003117.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96
Beta strandi15 – 228
Helixi27 – 326
Helixi33 – 4311
Beta strandi48 – 525
Beta strandi55 – 628
Beta strandi65 – 739
Helixi77 – 9418
Beta strandi95 – 973
Helixi100 – 1056
Helixi107 – 11711
Helixi128 – 1314
Turni132 – 1343
Helixi151 – 1544
Beta strandi155 – 1573
Beta strandi167 – 18317
Beta strandi185 – 1873
Beta strandi189 – 20315
Beta strandi205 – 2073
Beta strandi209 – 2146
Helixi217 – 2226
Beta strandi234 – 2385
Helixi244 – 2507
Beta strandi253 – 2553
Beta strandi259 – 26911
Beta strandi276 – 28712
Beta strandi290 – 29910
Beta strandi306 – 31510
Beta strandi321 – 33515
Turni336 – 3394
Beta strandi340 – 35112
Beta strandi353 – 3619
Beta strandi376 – 38813
Beta strandi392 – 3987
Beta strandi405 – 41410
Beta strandi416 – 4205

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1W63X-ray4.00M/N/O/P/R/V1-423[»]
4EMZX-ray2.90A/M158-423[»]
4EN2X-ray2.58A/M158-423[»]
4HMYX-ray7.00M1-423[»]
4P6ZX-ray3.00M1-423[»]
ProteinModelPortaliP35585.
SMRiP35585. Positions 2-423.

Miscellaneous databases

EvolutionaryTraceiP35585.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini168 – 421254MHD
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG315786.
HOGENOMiHOG000173247.
HOVERGENiHBG050516.
InParanoidiP35585.
KOiK12393.
OMAiYPWVRYI.
OrthoDBiEOG783MV6.
PhylomeDBiP35585.
TreeFamiTF300393.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR008968. Clathrin_mu_C.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35585-1 [UniParc]FASTAAdd to Basket

« Hide

MSASAVYVLD LKGKVLICRN YRGDVDMSEV EHFMPILMEK EEEGMLSPIL    50
AHGGVRFMWI KHNNLYLVAT SKKNACVSLV FSFLYKVVQV FSEYFKELEE 100
ESIRDNFVII YELLDELMDF GYPQTTDSKI LQEYITQEGH KLETGAPRPP 150
ATVTNAVSWR SEGIKYRKNE VFLDVIEAVN LLVSANGNVL RSEIVGSIKM 200
RVFLSGMPEL RLGLNDKVLF DNTGRGKSKS VELEDVKFHQ CVRLSRFEND 250
RTISFIPPDG EFELMSYRLN THVKPLIWIE SVIEKHSHSR IEYMVKAKSQ 300
FKRRSTANNV EIHIPVPNDA DSPKFKTTVG SVKWVPENSE IVWSVKSFPG 350
GKEYLMRAHF GLPSVEAEDK EGKPPISVKF EIPYFTTSGI QVRYLKIIEK 400
SGYQALPWVR YITQNGDYQL RTQ 423
Length:423
Mass (Da):48,543
Last modified:January 23, 2007 - v3
Checksum:i1213566B3A681B4C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62419 mRNA. Translation: AAA37244.1.
AF139405
, AF139394, AF139395, AF139396, AF139397, AF139398, AF139399, AF139400, AF139401, AF139402, AF139403, AF139404 Genomic DNA. Translation: AAF61814.1.
BC003823 mRNA. Translation: AAH03823.1.
CCDSiCCDS22411.1.
PIRiS19693.
RefSeqiNP_031482.1. NM_007456.4.
UniGeneiMm.290086.

Genome annotation databases

EnsembliENSMUST00000003117; ENSMUSP00000003117; ENSMUSG00000003033.
GeneIDi11767.
KEGGimmu:11767.
UCSCiuc009mfp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62419 mRNA. Translation: AAA37244.1 .
AF139405
, AF139394 , AF139395 , AF139396 , AF139397 , AF139398 , AF139399 , AF139400 , AF139401 , AF139402 , AF139403 , AF139404 Genomic DNA. Translation: AAF61814.1 .
BC003823 mRNA. Translation: AAH03823.1 .
CCDSi CCDS22411.1.
PIRi S19693.
RefSeqi NP_031482.1. NM_007456.4.
UniGenei Mm.290086.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1W63 X-ray 4.00 M/N/O/P/R/V 1-423 [» ]
4EMZ X-ray 2.90 A/M 158-423 [» ]
4EN2 X-ray 2.58 A/M 158-423 [» ]
4HMY X-ray 7.00 M 1-423 [» ]
4P6Z X-ray 3.00 M 1-423 [» ]
ProteinModelPortali P35585.
SMRi P35585. Positions 2-423.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198125. 1 interaction.
DIPi DIP-35323N.
IntActi P35585. 6 interactions.
MINTi MINT-216979.
STRINGi 10090.ENSMUSP00000003117.

PTM databases

PhosphoSitei P35585.

Proteomic databases

MaxQBi P35585.
PaxDbi P35585.
PRIDEi P35585.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000003117 ; ENSMUSP00000003117 ; ENSMUSG00000003033 .
GeneIDi 11767.
KEGGi mmu:11767.
UCSCi uc009mfp.1. mouse.

Organism-specific databases

CTDi 8907.
MGIi MGI:102776. Ap1m1.

Phylogenomic databases

eggNOGi NOG315786.
HOGENOMi HOG000173247.
HOVERGENi HBG050516.
InParanoidi P35585.
KOi K12393.
OMAi YPWVRYI.
OrthoDBi EOG783MV6.
PhylomeDBi P35585.
TreeFami TF300393.

Enzyme and pathway databases

Reactomei REACT_196550. MHC class II antigen presentation.

Miscellaneous databases

EvolutionaryTracei P35585.
NextBioi 279533.
PROi P35585.
SOURCEi Search...

Gene expression databases

ArrayExpressi P35585.
Bgeei P35585.
Genevestigatori P35585.

Family and domain databases

InterProi IPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR008968. Clathrin_mu_C.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view ]
Pfami PF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view ]
PIRSFi PIRSF005992. Clathrin_mu. 1 hit.
PRINTSi PR00314. CLATHRINADPT.
SUPFAMi SSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEi PS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast."
    Nakayama Y., Goebl M., O'Brine Greco B., Lemmon S., Pingchang C.E., Kirchhausen T.
    Eur. J. Biochem. 202:569-574(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genomic structure and chromosome mapping of the genes encoding clathrin-associated adaptor medium chains mu1A (Ap1m1) and mu1B (Ap1m2)."
    Nakatsu F., Kadohira T., Gilbert D.J., Jenkins N.A., Kakuta H., Copeland N.G., Saito T., Ohno H.
    Cytogenet. Cell Genet. 87:53-58(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-152 AND THR-154, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiAP1M1_MOUSE
AccessioniPrimary (citable) accession number: P35585
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi