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P35584

- FOXA3_MOUSE

UniProt

P35584 - FOXA3_MOUSE

Protein

Hepatocyte nuclear factor 3-gamma

Gene

Foxa3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Transcription factor that is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites By similarity. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; activates GLUT2 transcription. Involved in regulation of neuronal-specific transcription. Involved in regulation of spermatogenesis; required for the maintenance of the testicular germ cell population and male fertility.By similarity3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi118 – 20992Fork-headPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. sequence-specific DNA binding Source: MGI
    3. sequence-specific DNA binding transcription factor activity Source: InterPro
    4. transcription regulatory region DNA binding Source: Ensembl

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. cellular glucose homeostasis Source: MGI
    3. cellular response to starvation Source: MGI
    4. chromatin modification Source: UniProtKB-KW
    5. endocrine pancreas development Source: Reactome
    6. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    7. regulation of transcription, DNA-templated Source: MGI
    8. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    9. spermatogenesis Source: UniProtKB
    10. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Chromatin regulator, Developmental protein

    Keywords - Biological processi

    Differentiation, Spermatogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_214529. Regulation of gene expression in beta cells.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hepatocyte nuclear factor 3-gamma
    Short name:
    HNF-3-gamma
    Short name:
    HNF-3G
    Alternative name(s):
    Forkhead box protein A3
    Gene namesi
    Name:Foxa3
    Synonyms:Hnf3g, Tcf-3g, Tcf3g
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1347477. Foxa3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Reduced expression levels of several HNF3 target genes (phosphoenolpyruvate carboxykinase, transferrin, tyrosine aminotransferase) by 50 to 70%,. The germinal epithelium of testes is characterized by a loss of germ cells secondary to an increase in germ cell apoptosis that ultimately leads to a Sertoli cell-only syndrome. Significantly lower blood glucose in fasted mice.3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 353353Hepatocyte nuclear factor 3-gammaPRO_0000091801Add
    BLAST

    Proteomic databases

    PRIDEiP35584.

    PTM databases

    PhosphoSiteiP35584.

    Expressioni

    Tissue specificityi

    Restricted mainly to endoderm-derived tissues (lung, liver, stomach, and small intestine), also present additionally in ovary, testis, heart, and adipose tissue, but missing from lung.1 Publication

    Developmental stagei

    Expression peaks around day 15.5 of gestation. Expressed from day 6 to day 70 during postnatal testicular development.1 Publication

    Gene expression databases

    BgeeiP35584.
    CleanExiMM_FOXA3.
    GenevestigatoriP35584.

    Interactioni

    Subunit structurei

    Interacts with FOXA2.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000043173.

    Structurei

    3D structure databases

    ProteinModelPortaliP35584.
    SMRiP35584. Positions 117-217.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5025.
    GeneTreeiENSGT00740000115483.
    HOGENOMiHOG000231817.
    HOVERGENiHBG006621.
    InParanoidiP35584.
    KOiK08038.
    OMAiVYYQGVY.
    OrthoDBiEOG7C8GHD.
    PhylomeDBiP35584.
    TreeFamiTF316127.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR013638. Fork-head_N.
    IPR018533. Forkhead_box_C.
    IPR001766. TF_fork_head.
    IPR018122. TF_fork_head_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00250. Fork_head. 1 hit.
    PF08430. Fork_head_N. 1 hit.
    PF09354. HNF_C. 1 hit.
    [Graphical view]
    PRINTSiPR00053. FORKHEAD.
    SMARTiSM00339. FH. 1 hit.
    [Graphical view]
    PROSITEiPS00657. FORK_HEAD_1. 1 hit.
    PS00658. FORK_HEAD_2. 1 hit.
    PS50039. FORK_HEAD_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35584-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLGSVKMEAH DLAEWSYYPE AGEVYSPVNP VPTMAPLNSY MTLNPLSSPY    50
    PPGGLQASPL PTGPLAPPAP TAPLGPTFPS LGTGGSTGGS ASGYVAPGPG 100
    LVHGKEMAKG YRRPLAHAKP PYSYISLITM AIQQAPGKML TLSEIYQWIM 150
    DLFPYYRENQ QRWQNSIRHS LSFNDCFVKV ARSPDKPGKG SYWALHPSSG 200
    NMFENGCYLR RQKRFKLEEK AKKGNSATSA SRNGTAGSAT SATTTAATAV 250
    TSPAQPQPTP SEPEAQSGDD VGGLDCASPP SSTPYFSGLE LPGELKLDAP 300
    YNFNHPFSIN NLMSEQTSTP SKLDVGFGGY GAESGEPGVY YQSLYSRSLL 350
    NAS 353
    Length:353
    Mass (Da):37,601
    Last modified:June 1, 1994 - v1
    Checksum:i28F060A8E944D5B9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74938 mRNA. Translation: CAA52892.1.
    CCDSiCCDS20886.1.
    PIRiC54258.
    RefSeqiNP_032286.1. NM_008260.2.
    UniGeneiMm.42260.

    Genome annotation databases

    EnsembliENSMUST00000036018; ENSMUSP00000043173; ENSMUSG00000040891.
    GeneIDi15377.
    KEGGimmu:15377.
    UCSCiuc009fkd.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74938 mRNA. Translation: CAA52892.1 .
    CCDSi CCDS20886.1.
    PIRi C54258.
    RefSeqi NP_032286.1. NM_008260.2.
    UniGenei Mm.42260.

    3D structure databases

    ProteinModelPortali P35584.
    SMRi P35584. Positions 117-217.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000043173.

    PTM databases

    PhosphoSitei P35584.

    Proteomic databases

    PRIDEi P35584.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000036018 ; ENSMUSP00000043173 ; ENSMUSG00000040891 .
    GeneIDi 15377.
    KEGGi mmu:15377.
    UCSCi uc009fkd.1. mouse.

    Organism-specific databases

    CTDi 3171.
    MGIi MGI:1347477. Foxa3.

    Phylogenomic databases

    eggNOGi COG5025.
    GeneTreei ENSGT00740000115483.
    HOGENOMi HOG000231817.
    HOVERGENi HBG006621.
    InParanoidi P35584.
    KOi K08038.
    OMAi VYYQGVY.
    OrthoDBi EOG7C8GHD.
    PhylomeDBi P35584.
    TreeFami TF316127.

    Enzyme and pathway databases

    Reactomei REACT_214529. Regulation of gene expression in beta cells.

    Miscellaneous databases

    NextBioi 288034.
    PROi P35584.
    SOURCEi Search...

    Gene expression databases

    Bgeei P35584.
    CleanExi MM_FOXA3.
    Genevestigatori P35584.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR013638. Fork-head_N.
    IPR018533. Forkhead_box_C.
    IPR001766. TF_fork_head.
    IPR018122. TF_fork_head_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00250. Fork_head. 1 hit.
    PF08430. Fork_head_N. 1 hit.
    PF09354. HNF_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00053. FORKHEAD.
    SMARTi SM00339. FH. 1 hit.
    [Graphical view ]
    PROSITEi PS00657. FORK_HEAD_1. 1 hit.
    PS00658. FORK_HEAD_2. 1 hit.
    PS50039. FORK_HEAD_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The HNF-3 gene family of transcription factors in mice: gene structure, cDNA sequence, and mRNA distribution."
      Kaestner K., Hiemisch H., Luckow B., Schuetz G.
      Genomics 20:377-385(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Targeted disruption of the gene encoding hepatocyte nuclear factor 3gamma results in reduced transcription of hepatocyte-specific genes."
      Kaestner K.H., Hiemisch H., Schutz G.
      Mol. Cell. Biol. 18:4245-4251(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN LIVER-SPECIFIC TRANSCRIPTION, DISRUPTION PHENOTYPE.
    3. "Foxa3 (hepatocyte nuclear factor 3gamma) is required for the regulation of hepatic GLUT2 expression and the maintenance of glucose homeostasis during a prolonged fast."
      Shen W., Scearce L.M., Brestelli J.E., Sund N.J., Kaestner K.H.
      J. Biol. Chem. 276:42812-42817(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    4. "Impaired male fertility and atrophy of seminiferous tubules caused by haploinsufficiency for Foxa3."
      Behr R., Sackett S.D., Bochkis I.M., Le P.P., Kaestner K.H.
      Dev. Biol. 306:636-645(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, FUNCTION IN SPERMATOGENESIS.

    Entry informationi

    Entry nameiFOXA3_MOUSE
    AccessioniPrimary (citable) accession number: P35584
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3