Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P35584 (FOXA3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hepatocyte nuclear factor 3-gamma

Short name=HNF-3-gamma
Short name=HNF-3G
Alternative name(s):
Forkhead box protein A3
Gene names
Name:Foxa3
Synonyms:Hnf3g, Tcf-3g, Tcf3g
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites By similarity. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; activates GLUT2 transcription. Involved in regulation of neuronal-specific transcription. Involved in regulation of spermatogenesis; required for the maintenance of the testicular germ cell population and male fertility. Ref.2 Ref.3 Ref.4

Subunit structure

Interacts with FOXA2 By similarity.

Subcellular location

Nucleus.

Tissue specificity

Restricted mainly to endoderm-derived tissues (lung, liver, stomach, and small intestine), also present additionally in ovary, testis, heart, and adipose tissue, but missing from lung. Ref.4

Developmental stage

Expression peaks around day 15.5 of gestation. Expressed from day 6 to day 70 during postnatal testicular development. Ref.4

Disruption phenotype

Reduced expression levels of several HNF3 target genes (phosphoenolpyruvate carboxykinase, transferrin, tyrosine aminotransferase) by 50 to 70%,. The germinal epithelium of testes is characterized by a loss of germ cells secondary to an increase in germ cell apoptosis that ultimately leads to a Sertoli cell-only syndrome. Significantly lower blood glucose in fasted mice. Ref.2 Ref.3 Ref.4

Sequence similarities

Contains 1 fork-head DNA-binding domain.

Ontologies

Keywords
   Biological processDifferentiation
Spermatogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionActivator
Chromatin regulator
Developmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular glucose homeostasis

Inferred from mutant phenotype Ref.3. Source: MGI

cellular response to starvation

Inferred from mutant phenotype Ref.3. Source: MGI

chromatin modification

Inferred from electronic annotation. Source: UniProtKB-KW

embryo development

Inferred from Biological aspect of Ancestor. Source: RefGenome

endocrine pancreas development

Traceable author statement. Source: Reactome

negative regulation of transcription from RNA polymerase II promoter

Inferred from Biological aspect of Ancestor. Source: RefGenome

neuron fate specification

Inferred from Biological aspect of Ancestor. Source: RefGenome

pattern specification process

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of neuron differentiation

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 19759516. Source: MGI

regulation of hormone levels

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of sequence-specific DNA binding transcription factor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype Ref.4. Source: UniProtKB

regulation of transcription, DNA-templated

Inferred from mutant phenotype Ref.3. Source: MGI

spermatogenesis

Inferred from mutant phenotype Ref.4. Source: UniProtKB

tissue development

Inferred from Biological aspect of Ancestor. Source: RefGenome

transcription from RNA polymerase II promoter

Inferred from Biological aspect of Ancestor. Source: GOC

   Cellular_componentcytosol

Traceable author statement. Source: Reactome

nucleus

Inferred from direct assay PubMed 8306889. Source: MGI

transcription factor complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionDNA binding

Inferred from direct assay PubMed 7683413. Source: MGI

DNA binding, bending

Inferred from Biological aspect of Ancestor. Source: RefGenome

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

double-stranded DNA binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

sequence-specific DNA binding

Inferred from direct assay PubMed 8306889. Source: MGI

transcription factor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

transcription regulatory region DNA binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 353353Hepatocyte nuclear factor 3-gamma
PRO_0000091801

Regions

DNA binding118 – 20992Fork-head

Sequences

Sequence LengthMass (Da)Tools
P35584 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 28F060A8E944D5B9

FASTA35337,601
        10         20         30         40         50         60 
MLGSVKMEAH DLAEWSYYPE AGEVYSPVNP VPTMAPLNSY MTLNPLSSPY PPGGLQASPL 

        70         80         90        100        110        120 
PTGPLAPPAP TAPLGPTFPS LGTGGSTGGS ASGYVAPGPG LVHGKEMAKG YRRPLAHAKP 

       130        140        150        160        170        180 
PYSYISLITM AIQQAPGKML TLSEIYQWIM DLFPYYRENQ QRWQNSIRHS LSFNDCFVKV 

       190        200        210        220        230        240 
ARSPDKPGKG SYWALHPSSG NMFENGCYLR RQKRFKLEEK AKKGNSATSA SRNGTAGSAT 

       250        260        270        280        290        300 
SATTTAATAV TSPAQPQPTP SEPEAQSGDD VGGLDCASPP SSTPYFSGLE LPGELKLDAP 

       310        320        330        340        350 
YNFNHPFSIN NLMSEQTSTP SKLDVGFGGY GAESGEPGVY YQSLYSRSLL NAS 

« Hide

References

[1]"The HNF-3 gene family of transcription factors in mice: gene structure, cDNA sequence, and mRNA distribution."
Kaestner K., Hiemisch H., Luckow B., Schuetz G.
Genomics 20:377-385(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Targeted disruption of the gene encoding hepatocyte nuclear factor 3gamma results in reduced transcription of hepatocyte-specific genes."
Kaestner K.H., Hiemisch H., Schutz G.
Mol. Cell. Biol. 18:4245-4251(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN LIVER-SPECIFIC TRANSCRIPTION, DISRUPTION PHENOTYPE.
[3]"Foxa3 (hepatocyte nuclear factor 3gamma) is required for the regulation of hepatic GLUT2 expression and the maintenance of glucose homeostasis during a prolonged fast."
Shen W., Scearce L.M., Brestelli J.E., Sund N.J., Kaestner K.H.
J. Biol. Chem. 276:42812-42817(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[4]"Impaired male fertility and atrophy of seminiferous tubules caused by haploinsufficiency for Foxa3."
Behr R., Sackett S.D., Bochkis I.M., Le P.P., Kaestner K.H.
Dev. Biol. 306:636-645(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, FUNCTION IN SPERMATOGENESIS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X74938 mRNA. Translation: CAA52892.1.
PIRC54258.
RefSeqNP_032286.1. NM_008260.2.
UniGeneMm.42260.

3D structure databases

ProteinModelPortalP35584.
SMRP35584. Positions 117-217.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000043173.

PTM databases

PhosphoSiteP35584.

Proteomic databases

PRIDEP35584.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000036018; ENSMUSP00000043173; ENSMUSG00000040891.
GeneID15377.
KEGGmmu:15377.
UCSCuc009fkd.1. mouse.

Organism-specific databases

CTD3171.
MGIMGI:1347477. Foxa3.

Phylogenomic databases

eggNOGCOG5025.
GeneTreeENSGT00740000115483.
HOGENOMHOG000231817.
HOVERGENHBG006621.
InParanoidP35584.
KOK08038.
OMAVYYQGVY.
OrthoDBEOG7C8GHD.
PhylomeDBP35584.
TreeFamTF316127.

Enzyme and pathway databases

ReactomeREACT_13641. Regulation of Beta-Cell Development.
REACT_188576. Developmental Biology.

Gene expression databases

BgeeP35584.
CleanExMM_FOXA3.
GenevestigatorP35584.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR013638. Fork-head_N.
IPR018533. Forkhead_box_C.
IPR001766. TF_fork_head.
IPR018122. TF_fork_head_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00250. Fork_head. 1 hit.
PF08430. Fork_head_N. 1 hit.
PF09354. HNF_C. 1 hit.
[Graphical view]
PRINTSPR00053. FORKHEAD.
SMARTSM00339. FH. 1 hit.
[Graphical view]
PROSITEPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio288034.
PROP35584.
SOURCESearch...

Entry information

Entry nameFOXA3_MOUSE
AccessionPrimary (citable) accession number: P35584
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot