Reviewed, UniProtKB/Swiss-Prot P35576 (G6PC_MOUSE)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphatase
      Short name=G-6-Pase
      Short name=G6Pase
    EC=3.1.3.9

Gene names

Name:	G6pc
Synonyms:	G6pt

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels. Ref.3
Catalytic activity	D-glucose 6-phosphate + H2O = D-glucose + phosphate.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue specificity	Liver and kidney.
Sequence similarities	Belongs to the glucose-6-phosphatase family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cholesterol homeostasis Ref.3 Inferred from mutant phenotype. Source: MGI gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glucose 6-phosphate metabolic process Ref.3 Inferred from mutant phenotype. Source: MGI glucose homeostasis Ref.3 Inferred from mutant phenotype. Source: MGI glucose-6-phosphate transport Ref.3 Inferred from direct assay. Source: MGI glycogen catabolic process Inferred from mutant phenotype. Source: MGI multicellular organism growth Ref.3 Inferred from mutant phenotype. Source: MGI regulation of gene expression Inferred from mutant phenotype. Source: MGI steroid metabolic process Inferred from mutant phenotype. Source: MGI triglyceride metabolic process Ref.3 Inferred from mutant phenotype. Source: MGI urate metabolic process Ref.3 Inferred from mutant phenotype. Source: MGI
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from direct assay. Source: MGI
Molecular function	glucose-6-phosphatase activity Ref.1 Ref.3 Inferred from direct assay. Source: MGI phosphotransferase activity, alcohol group as acceptor Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Glucose-6-phosphatase		PRO_0000087414
Regions
	Topological domain	1 – 28	28	Lumenal Potential
	Transmembrane	29 – 49	21	Potential
	Topological domain	50 – 60	11	Cytoplasmic Potential
	Transmembrane	61 – 81	21	Potential
	Topological domain	82 – 117	36	Lumenal Potential
	Transmembrane	118 – 138	21	Potential
	Topological domain	139 – 147	9	Cytoplasmic Potential
	Transmembrane	148 – 168	21	Potential
	Topological domain	169 – 179	11	Lumenal Potential
	Transmembrane	180 – 202	23	Potential
	Topological domain	203 – 211	9	Cytoplasmic Potential
	Transmembrane	212 – 232	21	Potential
	Topological domain	233 – 254	22	Lumenal Potential
	Transmembrane	255 – 275	21	Potential
	Topological domain	276 – 291	16	Cytoplasmic Potential
	Transmembrane	292 – 312	21	Potential
	Topological domain	313 – 320	8	Lumenal Potential
	Transmembrane	321 – 341	21	Potential
	Topological domain	342 – 357	16	Cytoplasmic Potential
	Motif	354 – 357	4	Prevents secretion from ER Potential
Sites
	Active site	119	1	Proton donor Potential
	Active site	176	1	Nucleophile By similarity
	Binding site	83	1	Substrate Potential
	Binding site	170	1	Substrate Potential
Amino acid modifications
	Glycosylation	96	1	N-linked (GlcNAc...) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35576-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 292F9FCE39582692	FASTA	357	40,481
	10 20 30 40 50 60 MEEGMNILHD FGIQSTRYLQ VNYQDSQDWF ILVSVIADLR NAFYVLFPIW FHLKETVGIN 70 80 90 100 110 120 LLWVAVVGDW FNLVFKWILF GQRPYWWVLD TDYYSNSSVP IIKQFPVTCE TGPGSPSGHA 130 140 150 160 170 180 MGAAGVYYVM VTSTLAIFRG KKKPTYGFRC LNVILWLGFW AVQLNVCLSR IYLAAHFPHQ 190 200 210 220 230 240 VVAGVLSGIA VAETFSHIRG IYNASLRKYC LITIFLFGFA LGFYLLLKGL GVDLLWTLEK 250 260 270 280 290 300 AKRWCERPEW VHLDTTPFAS LFKNLGTLLG LGLALNSSMY RKSCKGELSK SFPFRFACIV 310 320 330 340 350 ASLVLLHLFD SLKPPSQVEL IFYILSFCKS ATVPFASVSL IPYCLARILG QTHKKSL

References

[1]	"Isolation of the gene for murine glucose-6-phosphatase, the enzyme deficient in glycogen storage disease type 1A." Shelly L.L., Lei K.-J., Pan C.-J., Sakata S.F., Ruppert S., Schutz G., Chou J.Y. J. Biol. Chem. 268:21482-21485(1993) [PubMed: 8407995] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"A multicomponent insulin response sequence mediates a strong repression of mouse glucose-6-phosphatase gene transcription by insulin." Streeper R.S., Svitek C.A., Chapman S., Greenbaum L.E., Taub R., O'Brien R.M. J. Biol. Chem. 272:11698-11701(1997) [PubMed: 9115220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-76. Strain: 129/Sv. Tissue: Liver.
[3]	"Glucose-6-phosphatase dependent substrate transport in the glycogen storage disease type-1a mouse." Lei K.-J., Chen H., Pan C.-J., Ward J.M., Mosinger B. Jr., Lee E.J., Westphal H., Mansfield B.C., Chou J.Y. Nat. Genet. 13:203-209(1996) [PubMed: 8640227] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00445 mRNA. Translation: AAC52122.1. U91573 Genomic DNA. Translation: AAC53166.1.
IPI	IPI00119645.
PIR	A48589.
UniGene	Mm.18064
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000078650. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:95607. G6pc.
Phylogenomic databases
HOVERGEN	P35576.
Enzyme and pathway databases
BRENDA	3.1.3.9. 244.
Gene expression databases
ArrayExpress	P35576.
Bgee	P35576.
CleanEx	MM_G6PC.
GermOnline	ENSMUSG00000019326. Mus musculus.
Family and domain databases
InterPro	IPR016275. Glucose-6-phosphatase. IPR000326. P_Acid_Pase_2/haloperoxidase. [Graphical view]
Pfam	PF01569. PAP2. 1 hit. [Graphical view]
PIRSF	PIRSF000905. Glucose-6-phosphatase. 1 hit.
SMART	SM00014. acidPPc. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

G6PC_MOUSE

Accession

Primary (citable) accession number: P35576

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	March 24, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)