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Protein

Glucose-6-phosphatase

Gene

G6pc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.1 Publication

Catalytic activityi

D-glucose 6-phosphate + H2O = D-glucose + phosphate.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei83SubstrateSequence analysis1
Active sitei119Proton donorSequence analysis1
Binding sitei170SubstrateSequence analysis1
Active sitei176NucleophileBy similarity1

GO - Molecular functioni

  • glucose-6-phosphatase activity Source: MGI
  • phosphate ion binding Source: MGI
  • phosphotransferase activity, alcohol group as acceptor Source: MGI

GO - Biological processi

  • cholesterol homeostasis Source: MGI
  • gluconeogenesis Source: MGI
  • glucose 6-phosphate metabolic process Source: MGI
  • glucose-6-phosphate transport Source: MGI
  • glucose homeostasis Source: MGI
  • glycogen catabolic process Source: MGI
  • glycogen metabolic process Source: MGI
  • multicellular organism growth Source: MGI
  • phosphorylated carbohydrate dephosphorylation Source: Ensembl
  • regulation of gene expression Source: MGI
  • response to food Source: Ensembl
  • steroid metabolic process Source: MGI
  • triglyceride metabolic process Source: MGI
  • urate metabolic process Source: MGI

Keywordsi

Molecular functionHydrolase
Biological processGluconeogenesis

Enzyme and pathway databases

BRENDAi3.1.3.9 3474
ReactomeiR-MMU-70263 Gluconeogenesis
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphatase (EC:3.1.3.9)
Short name:
G-6-Pase
Short name:
G6Pase
Gene namesi
Name:G6pc
Synonyms:G6pt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:95607 G6pc

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 28LumenalSequence analysisAdd BLAST28
Transmembranei29 – 49HelicalSequence analysisAdd BLAST21
Topological domaini50 – 60CytoplasmicSequence analysisAdd BLAST11
Transmembranei61 – 81HelicalSequence analysisAdd BLAST21
Topological domaini82 – 117LumenalSequence analysisAdd BLAST36
Transmembranei118 – 138HelicalSequence analysisAdd BLAST21
Topological domaini139 – 147CytoplasmicSequence analysis9
Transmembranei148 – 168HelicalSequence analysisAdd BLAST21
Topological domaini169 – 179LumenalSequence analysisAdd BLAST11
Transmembranei180 – 202HelicalSequence analysisAdd BLAST23
Topological domaini203 – 211CytoplasmicSequence analysis9
Transmembranei212 – 232HelicalSequence analysisAdd BLAST21
Topological domaini233 – 254LumenalSequence analysisAdd BLAST22
Transmembranei255 – 275HelicalSequence analysisAdd BLAST21
Topological domaini276 – 291CytoplasmicSequence analysisAdd BLAST16
Transmembranei292 – 312HelicalSequence analysisAdd BLAST21
Topological domaini313 – 320LumenalSequence analysis8
Transmembranei321 – 341HelicalSequence analysisAdd BLAST21
Topological domaini342 – 357CytoplasmicSequence analysisAdd BLAST16

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000874141 – 357Glucose-6-phosphataseAdd BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi96N-linked (GlcNAc...) asparagineBy similarity1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP35576
PaxDbiP35576
PRIDEiP35576

PTM databases

PhosphoSitePlusiP35576
SwissPalmiP35576

Expressioni

Tissue specificityi

Liver and kidney.

Gene expression databases

BgeeiENSMUSG00000078650
CleanExiMM_G6PC
GenevisibleiP35576 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000019469

Structurei

3D structure databases

ProteinModelPortaliP35576
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi354 – 357Prevents secretion from ERSequence analysis4

Sequence similaritiesi

Belongs to the glucose-6-phosphatase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IDXG Eukaryota
ENOG4110AJ7 LUCA
GeneTreeiENSGT00510000046465
HOGENOMiHOG000264239
HOVERGENiHBG003560
InParanoidiP35576
KOiK01084
OMAiYLQVNYQ
OrthoDBiEOG091G0AXF
TreeFamiTF324388

Family and domain databases

InterProiView protein in InterPro
IPR016275 Glucose-6-phosphatase
IPR036938 P_Acid_Pase_2/haloperoxi_sf
IPR000326 P_Acid_Pase_2/haloperoxidase
PfamiView protein in Pfam
PF01569 PAP2, 1 hit
PIRSFiPIRSF000905 Glucose-6-phosphatase, 1 hit
SMARTiView protein in SMART
SM00014 acidPPc, 1 hit
SUPFAMiSSF48317 SSF48317, 1 hit

Sequencei

Sequence statusi: Complete.

P35576-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEGMNILHD FGIQSTRYLQ VNYQDSQDWF ILVSVIADLR NAFYVLFPIW
60 70 80 90 100
FHLKETVGIN LLWVAVVGDW FNLVFKWILF GQRPYWWVLD TDYYSNSSVP
110 120 130 140 150
IIKQFPVTCE TGPGSPSGHA MGAAGVYYVM VTSTLAIFRG KKKPTYGFRC
160 170 180 190 200
LNVILWLGFW AVQLNVCLSR IYLAAHFPHQ VVAGVLSGIA VAETFSHIRG
210 220 230 240 250
IYNASLRKYC LITIFLFGFA LGFYLLLKGL GVDLLWTLEK AKRWCERPEW
260 270 280 290 300
VHLDTTPFAS LFKNLGTLLG LGLALNSSMY RKSCKGELSK LLPFRFACIV
310 320 330 340 350
ASLVLLHLFD SLKPPSQVEL IFYILSFCKS ATVPFASVSL IPYCLARILG

QTHKKSL
Length:357
Mass (Da):40,473
Last modified:July 27, 2011 - v2
Checksum:i292163ACA7A44402
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti291 – 292LL → SF in AAC52122 (PubMed:8407995).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00445 mRNA Translation: AAC52122.1
U91573 Genomic DNA Translation: AAC53166.1
AK050279 mRNA Translation: BAC34162.1
AK052656 mRNA Translation: BAC35084.1
AL590969 Genomic DNA Translation: CAM19548.1
BC013448 mRNA Translation: AAH13448.1
CCDSiCCDS25466.1
PIRiA48589
RefSeqiNP_032087.2, NM_008061.4
UniGeneiMm.18064

Genome annotation databases

EnsembliENSMUST00000019469; ENSMUSP00000019469; ENSMUSG00000078650
GeneIDi14377
KEGGimmu:14377
UCSCiuc007lor.1 mouse

Similar proteinsi

Entry informationi

Entry nameiG6PC_MOUSE
AccessioniPrimary (citable) accession number: P35576
Secondary accession number(s): Q91WV3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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