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P35576 (G6PC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphatase

Short name=G-6-Pase
Short name=G6Pase
EC=3.1.3.9
Gene names
Name:G6pc
Synonyms:G6pt
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels. Ref.6

Catalytic activity

D-glucose 6-phosphate + H2O = D-glucose + phosphate.

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Tissue specificity

Liver and kidney.

Sequence similarities

Belongs to the glucose-6-phosphatase family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentEndoplasmic reticulum
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcholesterol homeostasis

Inferred from mutant phenotype PubMed 10625614Ref.6. Source: MGI

dephosphorylation

Inferred from direct assay PubMed 10625614PubMed 10787428PubMed 11121425Ref.1. Source: GOC

gluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-UniPathway

glucose 6-phosphate metabolic process

Inferred from mutant phenotype Ref.6. Source: MGI

glucose homeostasis

Inferred from mutant phenotype PubMed 10625614Ref.6. Source: MGI

glucose-6-phosphate transport

Inferred from direct assay PubMed 10625614. Source: MGI

glycogen catabolic process

Inferred from mutant phenotype PubMed 10625614. Source: MGI

glycogen metabolic process

Inferred from mutant phenotype Ref.6. Source: MGI

multicellular organism growth

Inferred from mutant phenotype Ref.6. Source: MGI

regulation of gene expression

Inferred from mutant phenotype PubMed 17588937. Source: MGI

steroid metabolic process

Inferred from mutant phenotype PubMed 17588937. Source: MGI

triglyceride metabolic process

Inferred from mutant phenotype PubMed 10625614Ref.6. Source: MGI

urate metabolic process

Inferred from mutant phenotype Ref.6. Source: MGI

   Cellular_componentendoplasmic reticulum

Inferred from direct assay PubMed 10787428. Source: MGI

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionglucose-6-phosphatase activity

Inferred from direct assay PubMed 10625614PubMed 10787428PubMed 11121425Ref.1. Source: MGI

phosphate ion binding

Inferred from electronic annotation. Source: Ensembl

phosphotransferase activity, alcohol group as acceptor

Inferred from direct assay Ref.1. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357Glucose-6-phosphatase
PRO_0000087414

Regions

Topological domain1 – 2828Lumenal Potential
Transmembrane29 – 4921Helical; Potential
Topological domain50 – 6011Cytoplasmic Potential
Transmembrane61 – 8121Helical; Potential
Topological domain82 – 11736Lumenal Potential
Transmembrane118 – 13821Helical; Potential
Topological domain139 – 1479Cytoplasmic Potential
Transmembrane148 – 16821Helical; Potential
Topological domain169 – 17911Lumenal Potential
Transmembrane180 – 20223Helical; Potential
Topological domain203 – 2119Cytoplasmic Potential
Transmembrane212 – 23221Helical; Potential
Topological domain233 – 25422Lumenal Potential
Transmembrane255 – 27521Helical; Potential
Topological domain276 – 29116Cytoplasmic Potential
Transmembrane292 – 31221Helical; Potential
Topological domain313 – 3208Lumenal Potential
Transmembrane321 – 34121Helical; Potential
Topological domain342 – 35716Cytoplasmic Potential
Motif354 – 3574Prevents secretion from ER Potential

Sites

Active site1191Proton donor Potential
Active site1761Nucleophile By similarity
Binding site831Substrate Potential
Binding site1701Substrate Potential

Amino acid modifications

Glycosylation961N-linked (GlcNAc...) By similarity

Experimental info

Sequence conflict291 – 2922LL → SF in AAC52122. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P35576 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 292163ACA7A44402

FASTA35740,473
        10         20         30         40         50         60 
MEEGMNILHD FGIQSTRYLQ VNYQDSQDWF ILVSVIADLR NAFYVLFPIW FHLKETVGIN 

        70         80         90        100        110        120 
LLWVAVVGDW FNLVFKWILF GQRPYWWVLD TDYYSNSSVP IIKQFPVTCE TGPGSPSGHA 

       130        140        150        160        170        180 
MGAAGVYYVM VTSTLAIFRG KKKPTYGFRC LNVILWLGFW AVQLNVCLSR IYLAAHFPHQ 

       190        200        210        220        230        240 
VVAGVLSGIA VAETFSHIRG IYNASLRKYC LITIFLFGFA LGFYLLLKGL GVDLLWTLEK 

       250        260        270        280        290        300 
AKRWCERPEW VHLDTTPFAS LFKNLGTLLG LGLALNSSMY RKSCKGELSK LLPFRFACIV 

       310        320        330        340        350 
ASLVLLHLFD SLKPPSQVEL IFYILSFCKS ATVPFASVSL IPYCLARILG QTHKKSL 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of the gene for murine glucose-6-phosphatase, the enzyme deficient in glycogen storage disease type 1A."
Shelly L.L., Lei K.-J., Pan C.-J., Sakata S.F., Ruppert S., Schutz G., Chou J.Y.
J. Biol. Chem. 268:21482-21485(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Kidney and Liver.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Kidney.
[5]"A multicomponent insulin response sequence mediates a strong repression of mouse glucose-6-phosphatase gene transcription by insulin."
Streeper R.S., Svitek C.A., Chapman S., Greenbaum L.E., Taub R., O'Brien R.M.
J. Biol. Chem. 272:11698-11701(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-76.
Strain: 129/Sv.
Tissue: Liver.
[6]"Glucose-6-phosphatase dependent substrate transport in the glycogen storage disease type-1a mouse."
Lei K.-J., Chen H., Pan C.-J., Ward J.M., Mosinger B. Jr., Lee E.J., Westphal H., Mansfield B.C., Chou J.Y.
Nat. Genet. 13:203-209(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00445 mRNA. Translation: AAC52122.1.
U91573 Genomic DNA. Translation: AAC53166.1.
AK050279 mRNA. Translation: BAC34162.1.
AK052656 mRNA. Translation: BAC35084.1.
AL590969 Genomic DNA. Translation: CAM19548.1.
BC013448 mRNA. Translation: AAH13448.1.
CCDSCCDS25466.1.
PIRA48589.
RefSeqNP_032087.2. NM_008061.3.
UniGeneMm.18064.

3D structure databases

ProteinModelPortalP35576.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000019469.

PTM databases

PhosphoSiteP35576.

Proteomic databases

PaxDbP35576.
PRIDEP35576.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000019469; ENSMUSP00000019469; ENSMUSG00000078650.
GeneID14377.
KEGGmmu:14377.
UCSCuc007lor.1. mouse.

Organism-specific databases

CTD2538.
MGIMGI:95607. G6pc.

Phylogenomic databases

eggNOGNOG82628.
GeneTreeENSGT00510000046465.
HOGENOMHOG000264239.
HOVERGENHBG003560.
InParanoidQ91WV3.
KOK01084.
OMAYLQVNYQ.
OrthoDBEOG73NG4N.
TreeFamTF324388.

Enzyme and pathway databases

UniPathwayUPA00138.

Gene expression databases

BgeeP35576.
CleanExMM_G6PC.
GenevestigatorP35576.

Family and domain databases

Gene3D1.20.144.10. 1 hit.
InterProIPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamPF01569. PAP2. 1 hit.
[Graphical view]
PIRSFPIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMSSF48317. SSF48317. 1 hit.
ProtoNetSearch...

Other

ChiTaRSG6PC. mouse.
NextBio285881.
PROP35576.
SOURCESearch...

Entry information

Entry nameG6PC_MOUSE
AccessionPrimary (citable) accession number: P35576
Secondary accession number(s): Q91WV3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot