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P35576

- G6PC_MOUSE

UniProt

P35576 - G6PC_MOUSE

Protein

Glucose-6-phosphatase

Gene

G6pc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.1 Publication

    Catalytic activityi

    D-glucose 6-phosphate + H2O = D-glucose + phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei83 – 831SubstrateSequence Analysis
    Active sitei119 – 1191Proton donorSequence Analysis
    Binding sitei170 – 1701SubstrateSequence Analysis
    Active sitei176 – 1761NucleophileBy similarity

    GO - Molecular functioni

    1. glucose-6-phosphatase activity Source: MGI
    2. phosphate ion binding Source: Ensembl
    3. phosphotransferase activity, alcohol group as acceptor Source: MGI

    GO - Biological processi

    1. cholesterol homeostasis Source: MGI
    2. dephosphorylation Source: GOC
    3. gluconeogenesis Source: UniProtKB-UniPathway
    4. glucose 6-phosphate metabolic process Source: MGI
    5. glucose-6-phosphate transport Source: MGI
    6. glucose homeostasis Source: MGI
    7. glycogen catabolic process Source: MGI
    8. glycogen metabolic process Source: MGI
    9. multicellular organism growth Source: MGI
    10. regulation of gene expression Source: MGI
    11. steroid metabolic process Source: MGI
    12. triglyceride metabolic process Source: MGI
    13. urate metabolic process Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Gluconeogenesis

    Enzyme and pathway databases

    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphatase (EC:3.1.3.9)
    Short name:
    G-6-Pase
    Short name:
    G6Pase
    Gene namesi
    Name:G6pc
    Synonyms:G6pt
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:95607. G6pc.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: MGI
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 357357Glucose-6-phosphatasePRO_0000087414Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi96 – 961N-linked (GlcNAc...)By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP35576.
    PRIDEiP35576.

    PTM databases

    PhosphoSiteiP35576.

    Expressioni

    Tissue specificityi

    Liver and kidney.

    Gene expression databases

    BgeeiP35576.
    CleanExiMM_G6PC.
    GenevestigatoriP35576.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000019469.

    Structurei

    3D structure databases

    ProteinModelPortaliP35576.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2828LumenalSequence AnalysisAdd
    BLAST
    Topological domaini50 – 6011CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini82 – 11736LumenalSequence AnalysisAdd
    BLAST
    Topological domaini139 – 1479CytoplasmicSequence Analysis
    Topological domaini169 – 17911LumenalSequence AnalysisAdd
    BLAST
    Topological domaini203 – 2119CytoplasmicSequence Analysis
    Topological domaini233 – 25422LumenalSequence AnalysisAdd
    BLAST
    Topological domaini276 – 29116CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini313 – 3208LumenalSequence Analysis
    Topological domaini342 – 35716CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei29 – 4921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei61 – 8121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei118 – 13821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei148 – 16821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei180 – 20223HelicalSequence AnalysisAdd
    BLAST
    Transmembranei212 – 23221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei255 – 27521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei292 – 31221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei321 – 34121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi354 – 3574Prevents secretion from ERSequence Analysis

    Sequence similaritiesi

    Belongs to the glucose-6-phosphatase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG82628.
    GeneTreeiENSGT00510000046465.
    HOGENOMiHOG000264239.
    HOVERGENiHBG003560.
    InParanoidiQ91WV3.
    KOiK01084.
    OMAiYLQVNYQ.
    OrthoDBiEOG73NG4N.
    TreeFamiTF324388.

    Family and domain databases

    Gene3Di1.20.144.10. 1 hit.
    InterProiIPR016275. Glucose-6-phosphatase.
    IPR000326. P_Acid_Pase_2/haloperoxidase.
    [Graphical view]
    PfamiPF01569. PAP2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000905. Glucose-6-phosphatase. 1 hit.
    SMARTiSM00014. acidPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48317. SSF48317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P35576-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEGMNILHD FGIQSTRYLQ VNYQDSQDWF ILVSVIADLR NAFYVLFPIW    50
    FHLKETVGIN LLWVAVVGDW FNLVFKWILF GQRPYWWVLD TDYYSNSSVP 100
    IIKQFPVTCE TGPGSPSGHA MGAAGVYYVM VTSTLAIFRG KKKPTYGFRC 150
    LNVILWLGFW AVQLNVCLSR IYLAAHFPHQ VVAGVLSGIA VAETFSHIRG 200
    IYNASLRKYC LITIFLFGFA LGFYLLLKGL GVDLLWTLEK AKRWCERPEW 250
    VHLDTTPFAS LFKNLGTLLG LGLALNSSMY RKSCKGELSK LLPFRFACIV 300
    ASLVLLHLFD SLKPPSQVEL IFYILSFCKS ATVPFASVSL IPYCLARILG 350
    QTHKKSL 357
    Length:357
    Mass (Da):40,473
    Last modified:July 27, 2011 - v2
    Checksum:i292163ACA7A44402
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti291 – 2922LL → SF in AAC52122. (PubMed:8407995)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00445 mRNA. Translation: AAC52122.1.
    U91573 Genomic DNA. Translation: AAC53166.1.
    AK050279 mRNA. Translation: BAC34162.1.
    AK052656 mRNA. Translation: BAC35084.1.
    AL590969 Genomic DNA. Translation: CAM19548.1.
    BC013448 mRNA. Translation: AAH13448.1.
    CCDSiCCDS25466.1.
    PIRiA48589.
    RefSeqiNP_032087.2. NM_008061.3.
    UniGeneiMm.18064.

    Genome annotation databases

    EnsembliENSMUST00000019469; ENSMUSP00000019469; ENSMUSG00000078650.
    GeneIDi14377.
    KEGGimmu:14377.
    UCSCiuc007lor.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00445 mRNA. Translation: AAC52122.1 .
    U91573 Genomic DNA. Translation: AAC53166.1 .
    AK050279 mRNA. Translation: BAC34162.1 .
    AK052656 mRNA. Translation: BAC35084.1 .
    AL590969 Genomic DNA. Translation: CAM19548.1 .
    BC013448 mRNA. Translation: AAH13448.1 .
    CCDSi CCDS25466.1.
    PIRi A48589.
    RefSeqi NP_032087.2. NM_008061.3.
    UniGenei Mm.18064.

    3D structure databases

    ProteinModelPortali P35576.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000019469.

    PTM databases

    PhosphoSitei P35576.

    Proteomic databases

    PaxDbi P35576.
    PRIDEi P35576.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000019469 ; ENSMUSP00000019469 ; ENSMUSG00000078650 .
    GeneIDi 14377.
    KEGGi mmu:14377.
    UCSCi uc007lor.1. mouse.

    Organism-specific databases

    CTDi 2538.
    MGIi MGI:95607. G6pc.

    Phylogenomic databases

    eggNOGi NOG82628.
    GeneTreei ENSGT00510000046465.
    HOGENOMi HOG000264239.
    HOVERGENi HBG003560.
    InParanoidi Q91WV3.
    KOi K01084.
    OMAi YLQVNYQ.
    OrthoDBi EOG73NG4N.
    TreeFami TF324388.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .

    Miscellaneous databases

    ChiTaRSi G6PC. mouse.
    NextBioi 285881.
    PROi P35576.
    SOURCEi Search...

    Gene expression databases

    Bgeei P35576.
    CleanExi MM_G6PC.
    Genevestigatori P35576.

    Family and domain databases

    Gene3Di 1.20.144.10. 1 hit.
    InterProi IPR016275. Glucose-6-phosphatase.
    IPR000326. P_Acid_Pase_2/haloperoxidase.
    [Graphical view ]
    Pfami PF01569. PAP2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000905. Glucose-6-phosphatase. 1 hit.
    SMARTi SM00014. acidPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48317. SSF48317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of the gene for murine glucose-6-phosphatase, the enzyme deficient in glycogen storage disease type 1A."
      Shelly L.L., Lei K.-J., Pan C.-J., Sakata S.F., Ruppert S., Schutz G., Chou J.Y.
      J. Biol. Chem. 268:21482-21485(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Kidney and Liver.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney.
    5. "A multicomponent insulin response sequence mediates a strong repression of mouse glucose-6-phosphatase gene transcription by insulin."
      Streeper R.S., Svitek C.A., Chapman S., Greenbaum L.E., Taub R., O'Brien R.M.
      J. Biol. Chem. 272:11698-11701(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-76.
      Strain: 129/Sv.
      Tissue: Liver.
    6. "Glucose-6-phosphatase dependent substrate transport in the glycogen storage disease type-1a mouse."
      Lei K.-J., Chen H., Pan C.-J., Ward J.M., Mosinger B. Jr., Lee E.J., Westphal H., Mansfield B.C., Chou J.Y.
      Nat. Genet. 13:203-209(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiG6PC_MOUSE
    AccessioniPrimary (citable) accession number: P35576
    Secondary accession number(s): Q91WV3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3