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P35574 (GDE_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glycogen debranching enzyme
Alternative name(s):
Glycogen debrancher

Including the following 2 domains:

  1. 4-alpha-glucanotransferase
    EC=2.4.1.25
    Alternative name(s):
    Oligo-1,4-1,4-glucantransferase
  2. Amylo-alpha-1,6-glucosidase
    Short name=Amylo-1,6-glucosidase
    EC=3.2.1.33
    Alternative name(s):
    Dextrin 6-alpha-D-glucosidase
Gene names
Name:AGL
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length1555 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.

Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.

Subunit structure

Monomer. Interacts with NHLRC1/malin By similarity.

Subcellular location

Cytoplasm By similarity. Note: Under glycogenolytic conditions localizes to the nucleus By similarity.

Post-translational modification

The N-terminus is blocked.

Ubiquitinated By similarity.

Sequence similarities

Belongs to the glycogen debranching enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15551555Glycogen debranching enzyme
PRO_0000087451

Regions

Region1 – ?4-alpha-glucanotransferase
Region? – 1555Amylo-1,6-glucosidase

Sites

Active site5491 By similarity
Active site5521 By similarity
Active site6501 By similarity

Sequences

Sequence LengthMass (Da)Tools
P35574 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 7AD214297CB966E0

FASTA1,555177,656
        10         20         30         40         50         60 
MGNSFDFGVL LILLKYFKSS RSQNGHSKQI RILLLNEMEK LEKTLFRLEQ GFELQFRLGP 

        70         80         90        100        110        120 
TLQGKPVTVF TNYPFPGETF NREKFRSLEW ENPTEREDDS DKYCKLNLQQ SGSFQYYFLQ 

       130        140        150        160        170        180 
GNEKSGGGYI VVDPILRVGA DNHMLHLDCV TLQTFLAKCL GPFDEWESRL RVAKESGYNM 

       190        200        210        220        230        240 
IHFTPLQTLG LSRSCYSLAD QLELNPDFSR PHKKYTWSDV GQLVEKLKRE WNVLCITDVV 

       250        260        270        280        290        300 
YNHTAANSKW IQEHPECAYN LVNSPHLKPA WVLDRALWHF SCDVAEGKYK NRGVPALIEN 

       310        320        330        340        350        360 
DHHLNCIRKV IWEDIFPKLH LWEFFQVDVY KAVEKFRGLL TQETWRVIKS DPKQHLKIIQ 

       370        380        390        400        410        420 
DPEYRRFGCT VDMNIALATF IPHDNGPAAI EECCNWFRKR IEELNSEKHQ LMNYHQEQAV 

       430        440        450        460        470        480 
NCLLGNVFYE RLAGHGPKLG PVTRKYPLVT RYFTFPFEEM PVSTEETMIH LPNKACFFMA 

       490        500        510        520        530        540 
HNGWVMGDDP LRNFAEPGSD VYLRRELICW GDSVKLRYGT KPEDCPYLWA HMRKYTEIIA 

       550        560        570        580        590        600 
TYFQGVRLDN CHSTPLHVAE YMLDAARKLQ PNLYVVAELF TGSEDLDNIF VTRLGISSLI 

       610        620        630        640        650        660 
REAMSAYNSH EEGRLVYRYG GEPVGSFVQP CLRPLMPAIA HALFMDITHD NECPIVHRSV 

       670        680        690        700        710        720 
YDALPSTTIV SMACCASGST RGYDELVPHQ ISVVSEERFY TKWNPEALPS NAGEVNFQSG 

       730        740        750        760        770        780 
IIAARCAINK LHQELGAKGF IQVYVDQVDE DIVAVTRHSP SIHQSFVAVS RTAFRNPKTS 

       790        800        810        820        830        840 
FYSKDVPQMC IPGKIEEVVL EARTIERNIS PYRKDENSIN GMPNITVEIR EHIQLNESRI 

       850        860        870        880        890        900 
VKQAGVTTKG PNEYIQEIEF ENLSPGSVII FRVSLDPHAQ VAVGILRNHL TQFSAHFKAG 

       910        920        930        940        950        960 
SLAVDNSDPI LKIPFASIAS KLTLAEINQI LYRCESEEQE DGGGCYDIPN WSSLKYAGLQ 

       970        980        990       1000       1010       1020 
GLMSVLAEIR PKNDLGHPFC DNLRSGDWMI DYVSGRLISR SGTIAEVGKW LQAMFFYLKQ 

      1030       1040       1050       1060       1070       1080 
IPRYLIPCYF DAILIGAYTT LLDIAWKQMS SFVQTGSTFV KHLSLGSVQM CGVGKFPSLP 

      1090       1100       1110       1120       1130       1140 
LLSPSLTDVP YRLNEITKEK EQCCVSLAAG LPHFSSGIFR CWGRDTFIAL RGLLLITGRY 

      1150       1160       1170       1180       1190       1200 
LEARNIILAF AGTLRHGLIP NLLGEGTYAR YNCRDAVWWW LQCIQDYCKM VPNGLDILKC 

      1210       1220       1230       1240       1250       1260 
PVSRMYPTDD SAPLPAGTLD QPLFDVIQEA MQRHMQGIQF RERNAGPQID RNMKDEGFTV 

      1270       1280       1290       1300       1310       1320 
IAGVNEETGF VYGGNRFNCG TWMDKMGESD RARNRGIPAT PRDGSAVEIV GLCKSTVRWL 

      1330       1340       1350       1360       1370       1380 
LELSKKNIFP YHEVRVKRHG KVVTVSYEEW NRKIQDNFEK RFHVSEDPSA SNEEHPNLVH 

      1390       1400       1410       1420       1430       1440 
KRGIYKDSYG ASSPWCDYQL RPNFTIAMVV APELFTAEKA WKALEIAEKK LLGPLGMKTL 

      1450       1460       1470       1480       1490       1500 
DPDDMVYCGI YDNALDNDNY NLAKGFNYHQ GPEWLWPVGY FLRAKLYFSK LMDRETNART 

      1510       1520       1530       1540       1550 
IFLVKNVLSR HYVHLERSPW KGLPELTNEN GQYCPFSCET QAWSIATILE TLYDL 

« Hide

References

[1]"Molecular cloning, sequencing, and analysis of the cDNA for rabbit muscle glycogen debranching enzyme."
Liu W., de Castro M.L., Takrama J., Bilous P.T., Vinayagamoorthy T., Madsen N.B., Bleackley R.C.
Arch. Biochem. Biophys. 306:232-239(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Muscle.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L10605 mRNA. Translation: AAA16364.1.
PIRS38758.
RefSeqNP_001075716.1. NM_001082247.1.
UniGeneOcu.1807.

3D structure databases

ProteinModelPortalP35574.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9986.ENSOCUP00000008168.

Chemistry

BindingDBP35574.
ChEMBLCHEMBL5273.

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100009066.

Organism-specific databases

CTD178.

Phylogenomic databases

eggNOGCOG3408.
HOGENOMHOG000212981.
HOVERGENHBG005824.

Family and domain databases

Gene3D3.20.20.80. 5 hits.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR010401. AGL/Gdb1.
IPR029436. AGL_euk_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR006421. Glycogen_debranch_met.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10569. PTHR10569. 1 hit.
PfamPF06202. GDE_C. 1 hit.
PF14699. hGDE_N. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 2 hits.
SSF51445. SSF51445. 2 hits.
TIGRFAMsTIGR01531. glyc_debranch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGDE_RABIT
AccessionPrimary (citable) accession number: P35574
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families