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P35574

- GDE_RABIT

UniProt

P35574 - GDE_RABIT

Protein

Glycogen debranching enzyme

Gene

AGL

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.
    Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei549 – 5491By similarity
    Active sitei552 – 5521By similarity
    Active sitei650 – 6501By similarity

    GO - Molecular functioni

    1. 4-alpha-glucanotransferase activity Source: UniProtKB-EC
    2. amylo-alpha-1,6-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-KW
    2. glycogen catabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Glycosyltransferase, Hydrolase, Transferase

    Keywords - Biological processi

    Glycogen biosynthesis

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycogen debranching enzyme
    Alternative name(s):
    Glycogen debrancher
    Including the following 2 domains:
    4-alpha-glucanotransferase (EC:2.4.1.25)
    Alternative name(s):
    Oligo-1,4-1,4-glucantransferase
    Amylo-alpha-1,6-glucosidase (EC:3.2.1.33)
    Short name:
    Amylo-1,6-glucosidase
    Alternative name(s):
    Dextrin 6-alpha-D-glucosidase
    Gene namesi
    Name:AGL
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    Cytoplasm By similarity
    Note: Under glycogenolytic conditions localizes to the nucleus.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15551555Glycogen debranching enzymePRO_0000087451Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.
    Ubiquitinated.By similarity

    Keywords - PTMi

    Ubl conjugation

    Interactioni

    Subunit structurei

    Monomer. Interacts with NHLRC1/malin By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000008168.

    Structurei

    3D structure databases

    ProteinModelPortaliP35574.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni? – 1555Amylo-1,6-glucosidase
    Regioni1 – ?4-alpha-glucanotransferase

    Sequence similaritiesi

    Belongs to the glycogen debranching enzyme family.Curated

    Phylogenomic databases

    eggNOGiCOG3408.
    HOGENOMiHOG000212981.
    HOVERGENiHBG005824.

    Family and domain databases

    Gene3Di3.20.20.80. 5 hits.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR010401. AGL/Gdb1.
    IPR029436. AGL_euk_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR006421. Glycogen_debranch_met.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10569. PTHR10569. 1 hit.
    PfamiPF06202. GDE_C. 1 hit.
    PF14699. hGDE_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 2 hits.
    SSF51445. SSF51445. 2 hits.
    TIGRFAMsiTIGR01531. glyc_debranch. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P35574-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNSFDFGVL LILLKYFKSS RSQNGHSKQI RILLLNEMEK LEKTLFRLEQ     50
    GFELQFRLGP TLQGKPVTVF TNYPFPGETF NREKFRSLEW ENPTEREDDS 100
    DKYCKLNLQQ SGSFQYYFLQ GNEKSGGGYI VVDPILRVGA DNHMLHLDCV 150
    TLQTFLAKCL GPFDEWESRL RVAKESGYNM IHFTPLQTLG LSRSCYSLAD 200
    QLELNPDFSR PHKKYTWSDV GQLVEKLKRE WNVLCITDVV YNHTAANSKW 250
    IQEHPECAYN LVNSPHLKPA WVLDRALWHF SCDVAEGKYK NRGVPALIEN 300
    DHHLNCIRKV IWEDIFPKLH LWEFFQVDVY KAVEKFRGLL TQETWRVIKS 350
    DPKQHLKIIQ DPEYRRFGCT VDMNIALATF IPHDNGPAAI EECCNWFRKR 400
    IEELNSEKHQ LMNYHQEQAV NCLLGNVFYE RLAGHGPKLG PVTRKYPLVT 450
    RYFTFPFEEM PVSTEETMIH LPNKACFFMA HNGWVMGDDP LRNFAEPGSD 500
    VYLRRELICW GDSVKLRYGT KPEDCPYLWA HMRKYTEIIA TYFQGVRLDN 550
    CHSTPLHVAE YMLDAARKLQ PNLYVVAELF TGSEDLDNIF VTRLGISSLI 600
    REAMSAYNSH EEGRLVYRYG GEPVGSFVQP CLRPLMPAIA HALFMDITHD 650
    NECPIVHRSV YDALPSTTIV SMACCASGST RGYDELVPHQ ISVVSEERFY 700
    TKWNPEALPS NAGEVNFQSG IIAARCAINK LHQELGAKGF IQVYVDQVDE 750
    DIVAVTRHSP SIHQSFVAVS RTAFRNPKTS FYSKDVPQMC IPGKIEEVVL 800
    EARTIERNIS PYRKDENSIN GMPNITVEIR EHIQLNESRI VKQAGVTTKG 850
    PNEYIQEIEF ENLSPGSVII FRVSLDPHAQ VAVGILRNHL TQFSAHFKAG 900
    SLAVDNSDPI LKIPFASIAS KLTLAEINQI LYRCESEEQE DGGGCYDIPN 950
    WSSLKYAGLQ GLMSVLAEIR PKNDLGHPFC DNLRSGDWMI DYVSGRLISR 1000
    SGTIAEVGKW LQAMFFYLKQ IPRYLIPCYF DAILIGAYTT LLDIAWKQMS 1050
    SFVQTGSTFV KHLSLGSVQM CGVGKFPSLP LLSPSLTDVP YRLNEITKEK 1100
    EQCCVSLAAG LPHFSSGIFR CWGRDTFIAL RGLLLITGRY LEARNIILAF 1150
    AGTLRHGLIP NLLGEGTYAR YNCRDAVWWW LQCIQDYCKM VPNGLDILKC 1200
    PVSRMYPTDD SAPLPAGTLD QPLFDVIQEA MQRHMQGIQF RERNAGPQID 1250
    RNMKDEGFTV IAGVNEETGF VYGGNRFNCG TWMDKMGESD RARNRGIPAT 1300
    PRDGSAVEIV GLCKSTVRWL LELSKKNIFP YHEVRVKRHG KVVTVSYEEW 1350
    NRKIQDNFEK RFHVSEDPSA SNEEHPNLVH KRGIYKDSYG ASSPWCDYQL 1400
    RPNFTIAMVV APELFTAEKA WKALEIAEKK LLGPLGMKTL DPDDMVYCGI 1450
    YDNALDNDNY NLAKGFNYHQ GPEWLWPVGY FLRAKLYFSK LMDRETNART 1500
    IFLVKNVLSR HYVHLERSPW KGLPELTNEN GQYCPFSCET QAWSIATILE 1550
    TLYDL 1555
    Length:1,555
    Mass (Da):177,656
    Last modified:June 1, 1994 - v1
    Checksum:i7AD214297CB966E0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10605 mRNA. Translation: AAA16364.1.
    PIRiS38758.
    RefSeqiNP_001075716.1. NM_001082247.1.
    UniGeneiOcu.1807.

    Genome annotation databases

    GeneIDi100009066.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10605 mRNA. Translation: AAA16364.1 .
    PIRi S38758.
    RefSeqi NP_001075716.1. NM_001082247.1.
    UniGenei Ocu.1807.

    3D structure databases

    ProteinModelPortali P35574.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000008168.

    Chemistry

    BindingDBi P35574.
    ChEMBLi CHEMBL5273.

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100009066.

    Organism-specific databases

    CTDi 178.

    Phylogenomic databases

    eggNOGi COG3408.
    HOGENOMi HOG000212981.
    HOVERGENi HBG005824.

    Family and domain databases

    Gene3Di 3.20.20.80. 5 hits.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR010401. AGL/Gdb1.
    IPR029436. AGL_euk_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR006421. Glycogen_debranch_met.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10569. PTHR10569. 1 hit.
    Pfami PF06202. GDE_C. 1 hit.
    PF14699. hGDE_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 2 hits.
    SSF51445. SSF51445. 2 hits.
    TIGRFAMsi TIGR01531. glyc_debranch. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, sequencing, and analysis of the cDNA for rabbit muscle glycogen debranching enzyme."
      Liu W., de Castro M.L., Takrama J., Bilous P.T., Vinayagamoorthy T., Madsen N.B., Bleackley R.C.
      Arch. Biochem. Biophys. 306:232-239(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Muscle.

    Entry informationi

    Entry nameiGDE_RABIT
    AccessioniPrimary (citable) accession number: P35574
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3