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P35574

- GDE_RABIT

UniProt

P35574 - GDE_RABIT

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Protein
Glycogen debranching enzyme
Gene
AGL
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.
Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei549 – 5491 By similarity
Active sitei552 – 5521 By similarity
Active sitei650 – 6501 By similarity

GO - Molecular functioni

  1. 4-alpha-glucanotransferase activity Source: UniProtKB-EC
  2. amylo-alpha-1,6-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-KW
  2. glycogen catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen debranching enzyme
Alternative name(s):
Glycogen debrancher
Including the following 2 domains:
4-alpha-glucanotransferase (EC:2.4.1.25)
Alternative name(s):
Oligo-1,4-1,4-glucantransferase
Amylo-alpha-1,6-glucosidase (EC:3.2.1.33)
Short name:
Amylo-1,6-glucosidase
Alternative name(s):
Dextrin 6-alpha-D-glucosidase
Gene namesi
Name:AGL
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

Cytoplasm By similarity
Note: Under glycogenolytic conditions localizes to the nucleus By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15551555Glycogen debranching enzyme
PRO_0000087451Add
BLAST

Post-translational modificationi

The N-terminus is blocked.
Ubiquitinated By similarity.

Keywords - PTMi

Ubl conjugation

Interactioni

Subunit structurei

Monomer. Interacts with NHLRC1/malin By similarity.

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000008168.

Structurei

3D structure databases

ProteinModelPortaliP35574.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni? – 1555Amylo-1,6-glucosidase
Regioni1 – ?4-alpha-glucanotransferase

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3408.
HOGENOMiHOG000212981.
HOVERGENiHBG005824.

Family and domain databases

Gene3Di3.20.20.80. 5 hits.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR010401. AGL/Gdb1.
IPR029436. AGL_euk_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR006421. Glycogen_debranch_met.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10569. PTHR10569. 1 hit.
PfamiPF06202. GDE_C. 1 hit.
PF14699. hGDE_N. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 2 hits.
SSF51445. SSF51445. 2 hits.
TIGRFAMsiTIGR01531. glyc_debranch. 1 hit.

Sequencei

Sequence statusi: Complete.

P35574-1 [UniParc]FASTAAdd to Basket

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MGNSFDFGVL LILLKYFKSS RSQNGHSKQI RILLLNEMEK LEKTLFRLEQ     50
GFELQFRLGP TLQGKPVTVF TNYPFPGETF NREKFRSLEW ENPTEREDDS 100
DKYCKLNLQQ SGSFQYYFLQ GNEKSGGGYI VVDPILRVGA DNHMLHLDCV 150
TLQTFLAKCL GPFDEWESRL RVAKESGYNM IHFTPLQTLG LSRSCYSLAD 200
QLELNPDFSR PHKKYTWSDV GQLVEKLKRE WNVLCITDVV YNHTAANSKW 250
IQEHPECAYN LVNSPHLKPA WVLDRALWHF SCDVAEGKYK NRGVPALIEN 300
DHHLNCIRKV IWEDIFPKLH LWEFFQVDVY KAVEKFRGLL TQETWRVIKS 350
DPKQHLKIIQ DPEYRRFGCT VDMNIALATF IPHDNGPAAI EECCNWFRKR 400
IEELNSEKHQ LMNYHQEQAV NCLLGNVFYE RLAGHGPKLG PVTRKYPLVT 450
RYFTFPFEEM PVSTEETMIH LPNKACFFMA HNGWVMGDDP LRNFAEPGSD 500
VYLRRELICW GDSVKLRYGT KPEDCPYLWA HMRKYTEIIA TYFQGVRLDN 550
CHSTPLHVAE YMLDAARKLQ PNLYVVAELF TGSEDLDNIF VTRLGISSLI 600
REAMSAYNSH EEGRLVYRYG GEPVGSFVQP CLRPLMPAIA HALFMDITHD 650
NECPIVHRSV YDALPSTTIV SMACCASGST RGYDELVPHQ ISVVSEERFY 700
TKWNPEALPS NAGEVNFQSG IIAARCAINK LHQELGAKGF IQVYVDQVDE 750
DIVAVTRHSP SIHQSFVAVS RTAFRNPKTS FYSKDVPQMC IPGKIEEVVL 800
EARTIERNIS PYRKDENSIN GMPNITVEIR EHIQLNESRI VKQAGVTTKG 850
PNEYIQEIEF ENLSPGSVII FRVSLDPHAQ VAVGILRNHL TQFSAHFKAG 900
SLAVDNSDPI LKIPFASIAS KLTLAEINQI LYRCESEEQE DGGGCYDIPN 950
WSSLKYAGLQ GLMSVLAEIR PKNDLGHPFC DNLRSGDWMI DYVSGRLISR 1000
SGTIAEVGKW LQAMFFYLKQ IPRYLIPCYF DAILIGAYTT LLDIAWKQMS 1050
SFVQTGSTFV KHLSLGSVQM CGVGKFPSLP LLSPSLTDVP YRLNEITKEK 1100
EQCCVSLAAG LPHFSSGIFR CWGRDTFIAL RGLLLITGRY LEARNIILAF 1150
AGTLRHGLIP NLLGEGTYAR YNCRDAVWWW LQCIQDYCKM VPNGLDILKC 1200
PVSRMYPTDD SAPLPAGTLD QPLFDVIQEA MQRHMQGIQF RERNAGPQID 1250
RNMKDEGFTV IAGVNEETGF VYGGNRFNCG TWMDKMGESD RARNRGIPAT 1300
PRDGSAVEIV GLCKSTVRWL LELSKKNIFP YHEVRVKRHG KVVTVSYEEW 1350
NRKIQDNFEK RFHVSEDPSA SNEEHPNLVH KRGIYKDSYG ASSPWCDYQL 1400
RPNFTIAMVV APELFTAEKA WKALEIAEKK LLGPLGMKTL DPDDMVYCGI 1450
YDNALDNDNY NLAKGFNYHQ GPEWLWPVGY FLRAKLYFSK LMDRETNART 1500
IFLVKNVLSR HYVHLERSPW KGLPELTNEN GQYCPFSCET QAWSIATILE 1550
TLYDL 1555
Length:1,555
Mass (Da):177,656
Last modified:June 1, 1994 - v1
Checksum:i7AD214297CB966E0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10605 mRNA. Translation: AAA16364.1.
PIRiS38758.
RefSeqiNP_001075716.1. NM_001082247.1.
UniGeneiOcu.1807.

Genome annotation databases

GeneIDi100009066.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10605 mRNA. Translation: AAA16364.1 .
PIRi S38758.
RefSeqi NP_001075716.1. NM_001082247.1.
UniGenei Ocu.1807.

3D structure databases

ProteinModelPortali P35574.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9986.ENSOCUP00000008168.

Chemistry

BindingDBi P35574.
ChEMBLi CHEMBL5273.

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100009066.

Organism-specific databases

CTDi 178.

Phylogenomic databases

eggNOGi COG3408.
HOGENOMi HOG000212981.
HOVERGENi HBG005824.

Family and domain databases

Gene3Di 3.20.20.80. 5 hits.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR010401. AGL/Gdb1.
IPR029436. AGL_euk_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR006421. Glycogen_debranch_met.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10569. PTHR10569. 1 hit.
Pfami PF06202. GDE_C. 1 hit.
PF14699. hGDE_N. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 2 hits.
SSF51445. SSF51445. 2 hits.
TIGRFAMsi TIGR01531. glyc_debranch. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning, sequencing, and analysis of the cDNA for rabbit muscle glycogen debranching enzyme."
    Liu W., de Castro M.L., Takrama J., Bilous P.T., Vinayagamoorthy T., Madsen N.B., Bleackley R.C.
    Arch. Biochem. Biophys. 306:232-239(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Muscle.

Entry informationi

Entry nameiGDE_RABIT
AccessioniPrimary (citable) accession number: P35574
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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