P35574 (GDE_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycogen debranching enzyme

Alternative name(s):
Glycogen debrancher

Including the following 2 domains:

1. 4-alpha-glucanotransferase
   EC=2.4.1.25
   Alternative name(s):
   Oligo-1,4-1,4-glucantransferase
2. Amylo-alpha-1,6-glucosidase
   Short name=Amylo-1,6-glucosidase
   EC=3.2.1.33
   Alternative name(s):
   Dextrin 6-alpha-D-glucosidase

Gene names

Name:

AGL

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	1555 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan. Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.
Subunit structure	Monomer. Interacts with NHLRC1/malin By similarity.
Subcellular location	Cytoplasm By similarity. Note: Under glycogenolytic conditions localizes to the nucleus By similarity.
Post-translational modification	The N-terminus is blocked. Ubiquitinated By similarity.
Sequence similarities	Belongs to the glycogen debranching enzyme family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Cellular component	Cytoplasm
Molecular function	Glycosidase Glycosyltransferase Hydrolase Transferase
PTM	Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	4-alpha-glucanotransferase activity Inferred from electronic annotation. Source: EC amylo-alpha-1,6-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1555	1555	Glycogen debranching enzyme		PRO_0000087451
Regions
	Region	1 – ?		4-alpha-glucanotransferase
	Region	? – 1555		Amylo-1,6-glucosidase
Sites
	Active site	549	1	By similarity
	Active site	552	1	By similarity
	Active site	650	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35574 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 7AD214297CB966E0	FASTA	1,555	177,656
	10 20 30 40 50 60 MGNSFDFGVL LILLKYFKSS RSQNGHSKQI RILLLNEMEK LEKTLFRLEQ GFELQFRLGP 70 80 90 100 110 120 TLQGKPVTVF TNYPFPGETF NREKFRSLEW ENPTEREDDS DKYCKLNLQQ SGSFQYYFLQ 130 140 150 160 170 180 GNEKSGGGYI VVDPILRVGA DNHMLHLDCV TLQTFLAKCL GPFDEWESRL RVAKESGYNM 190 200 210 220 230 240 IHFTPLQTLG LSRSCYSLAD QLELNPDFSR PHKKYTWSDV GQLVEKLKRE WNVLCITDVV 250 260 270 280 290 300 YNHTAANSKW IQEHPECAYN LVNSPHLKPA WVLDRALWHF SCDVAEGKYK NRGVPALIEN 310 320 330 340 350 360 DHHLNCIRKV IWEDIFPKLH LWEFFQVDVY KAVEKFRGLL TQETWRVIKS DPKQHLKIIQ 370 380 390 400 410 420 DPEYRRFGCT VDMNIALATF IPHDNGPAAI EECCNWFRKR IEELNSEKHQ LMNYHQEQAV 430 440 450 460 470 480 NCLLGNVFYE RLAGHGPKLG PVTRKYPLVT RYFTFPFEEM PVSTEETMIH LPNKACFFMA 490 500 510 520 530 540 HNGWVMGDDP LRNFAEPGSD VYLRRELICW GDSVKLRYGT KPEDCPYLWA HMRKYTEIIA 550 560 570 580 590 600 TYFQGVRLDN CHSTPLHVAE YMLDAARKLQ PNLYVVAELF TGSEDLDNIF VTRLGISSLI 610 620 630 640 650 660 REAMSAYNSH EEGRLVYRYG GEPVGSFVQP CLRPLMPAIA HALFMDITHD NECPIVHRSV 670 680 690 700 710 720 YDALPSTTIV SMACCASGST RGYDELVPHQ ISVVSEERFY TKWNPEALPS NAGEVNFQSG 730 740 750 760 770 780 IIAARCAINK LHQELGAKGF IQVYVDQVDE DIVAVTRHSP SIHQSFVAVS RTAFRNPKTS 790 800 810 820 830 840 FYSKDVPQMC IPGKIEEVVL EARTIERNIS PYRKDENSIN GMPNITVEIR EHIQLNESRI 850 860 870 880 890 900 VKQAGVTTKG PNEYIQEIEF ENLSPGSVII FRVSLDPHAQ VAVGILRNHL TQFSAHFKAG 910 920 930 940 950 960 SLAVDNSDPI LKIPFASIAS KLTLAEINQI LYRCESEEQE DGGGCYDIPN WSSLKYAGLQ 970 980 990 1000 1010 1020 GLMSVLAEIR PKNDLGHPFC DNLRSGDWMI DYVSGRLISR SGTIAEVGKW LQAMFFYLKQ 1030 1040 1050 1060 1070 1080 IPRYLIPCYF DAILIGAYTT LLDIAWKQMS SFVQTGSTFV KHLSLGSVQM CGVGKFPSLP 1090 1100 1110 1120 1130 1140 LLSPSLTDVP YRLNEITKEK EQCCVSLAAG LPHFSSGIFR CWGRDTFIAL RGLLLITGRY 1150 1160 1170 1180 1190 1200 LEARNIILAF AGTLRHGLIP NLLGEGTYAR YNCRDAVWWW LQCIQDYCKM VPNGLDILKC 1210 1220 1230 1240 1250 1260 PVSRMYPTDD SAPLPAGTLD QPLFDVIQEA MQRHMQGIQF RERNAGPQID RNMKDEGFTV 1270 1280 1290 1300 1310 1320 IAGVNEETGF VYGGNRFNCG TWMDKMGESD RARNRGIPAT PRDGSAVEIV GLCKSTVRWL 1330 1340 1350 1360 1370 1380 LELSKKNIFP YHEVRVKRHG KVVTVSYEEW NRKIQDNFEK RFHVSEDPSA SNEEHPNLVH 1390 1400 1410 1420 1430 1440 KRGIYKDSYG ASSPWCDYQL RPNFTIAMVV APELFTAEKA WKALEIAEKK LLGPLGMKTL 1450 1460 1470 1480 1490 1500 DPDDMVYCGI YDNALDNDNY NLAKGFNYHQ GPEWLWPVGY FLRAKLYFSK LMDRETNART 1510 1520 1530 1540 1550 IFLVKNVLSR HYVHLERSPW KGLPELTNEN GQYCPFSCET QAWSIATILE TLYDL

References

[1]

"Molecular cloning, sequencing, and analysis of the cDNA for rabbit muscle glycogen debranching enzyme." Liu W., de Castro M.L., Takrama J., Bilous P.T., Vinayagamoorthy T., Madsen N.B., Bleackley R.C. Arch. Biochem. Biophys. 306:232-239(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Muscle.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L10605 mRNA. Translation: AAA16364.1.
PIR	S38758.
RefSeq	NP_001075716.1. NM_001082247.1.
UniGene	Ocu.1807.
3D structure databases
ProteinModelPortal	P35574.
ModBase	Search...
Protein-protein interaction databases
STRING	9986.ENSOCUP00000008168.
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100009066.
Organism-specific databases
CTD	178.
Phylogenomic databases
eggNOG	COG3408.
HOGENOM	HOG000212981.
HOVERGEN	HBG005824.
Family and domain databases
Gene3D	3.20.20.80. 5 hits.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR010401. GDE_C. IPR013781. Glyco_hydro_catalytic_dom. IPR006421. Glycogen_debranch_met. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10569. PTHR10569. 1 hit.
Pfam	PF06202. GDE_C. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF48208. Glyco_trans_6hp. 1 hit.
TIGRFAMs	TIGR01531. glyc_debranch. 1 hit.
ProtoNet	Search...
Other
BindingDB	P35574.
ChEMBL	CHEMBL5273.

Entry information

Entry name

GDE_RABIT

Accession

Primary (citable) accession number: P35574

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program