P35572 (IBP6_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like growth factor-binding protein 6
Short name=IBP-6
Short name=IGF-binding protein 6
Short name=IGFBP-6

Gene names

Name:	Igfbp6
Synonyms:	Igfbp-6

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Subcellular location	Secreted.
Post-translational modification	O-glycosylated.
Sequence similarities	Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Growth factor binding
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of cell growth Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 14515325. Source: RGD extracellular space Inferred from direct assay PubMed 7689963. Source: RGD
Molecular_function	insulin-like growth factor II binding Traceable author statement PubMed 12175646. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Ref.4
	Chain	26 – 226	201	Insulin-like growth factor-binding protein 6		PRO_0000014391
Regions
	Domain	26 – 99	74	IGFBP N-terminal
	Domain	145 – 220	76	Thyroglobulin type-1
Amino acid modifications
	Disulfide bond	30 ↔ 33		By similarity
	Disulfide bond	49 ↔ 55		By similarity
	Disulfide bond	63 ↔ 76		By similarity
	Disulfide bond	70 ↔ 96		By similarity
	Disulfide bond	148 ↔ 176		By similarity
	Disulfide bond	187 ↔ 198		By similarity
	Disulfide bond	200 ↔ 220		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35572 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 212D419303AE3137	FASTA	226	24,193
	10 20 30 40 50 60 MTWDGLPTQP LLMLLMLLFA AGSESALAGC PGCGPGVQEE DAGSPADGCA ETGGCFRREG 70 80 90 100 110 120 QPCGVYIPKC APGLQCQPRE NEETPLRALL IGQGRCQRAR GPSEETTKES KPHGGASRPR 130 140 150 160 170 180 DRDRQKNPRT SAAPIRPSPV QDGEMGPCRR HLDSVLQQLQ TEVFRGGANG LYVPNCDLRG 190 200 210 220 FYRKQQCRSS QGNRRGPCWC VDPMGQPLPV SPDGQGSSQC SARSSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and molecular cloning of insulin-like growth factor-binding protein-6." Shimasaki S., Gao L., Shimonaka M., Ling N. Mol. Endocrinol. 5:938-948(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural characterization of the rat insulin-like growth factor binding protein-6 gene." Zhu X., Ling N., Shimasaki S. Biochem. Biophys. Res. Commun. 191:1237-1243(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[4]	"Identification of five different insulin-like growth factor binding proteins (IGFBPs) from adult rat serum and molecular cloning of a novel IGFBP-5 in rat and human." Shimasaki S., Shimonaka M., Zhang H.-P., Ling N. J. Biol. Chem. 266:10646-10653(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-38.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M69055 mRNA. Translation: AAA42019.1. L11006 Genomic DNA. No translation available. BC099742 mRNA. Translation: AAH99742.1.
IPI	IPI00199664.
PIR	JN0464.
RefSeq	NP_037236.1. NM_013104.3.
UniGene	Rn.6431.
3D structure databases
ProteinModelPortal	P35572.
SMR	P35572. Positions 26-96, 146-226.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000014807.
Protein family/group databases
MEROPS	I31.952.
Proteomic databases
PaxDb	P35572.
PRIDE	P35572.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000014807; ENSRNOP00000014807; ENSRNOG00000010977.
GeneID	25641.
KEGG	rno:25641.
Organism-specific databases
CTD	3489.
RGD	2877. Igfbp6.
Phylogenomic databases
eggNOG	NOG47794.
GeneTree	ENSGT00550000074457.
HOGENOM	HOG000253012.
HOVERGEN	HBG002631.
InParanoid	P35572.
OMA	PNPGGVQ.
OrthoDB	EOG4W0XF4.
Gene expression databases
Genevestigator	P35572.
GermOnline	ENSRNOG00000010977. Rattus norvegicus.
Family and domain databases
Gene3D	4.10.40.20. 1 hit. 4.10.800.10. 1 hit.
InterPro	IPR009030. Growth_fac_rcpt_N_dom. IPR022326. IGFBP-6. IPR000867. IGFBP-like. IPR009168. IGFBP1-6. IPR022321. IGFBP_1-6_chordata. IPR000716. Thyroglobulin_1. [Graphical view]
PANTHER	PTHR11551. PTHR11551. 1 hit.
Pfam	PF00219. IGFBP. 1 hit. PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
PRINTS	PR01976. IGFBPFAMILY. PR01982. IGFBPFAMILY6.
SMART	SM00121. IB. 1 hit. SM00211. TY. 1 hit. [Graphical view]
SUPFAM	SSF57184. Grow_fac_recept. 1 hit. SSF57610. Thyroglobulin_1. 1 hit.
PROSITE	PS00222. IGFBP_N_1. False negative. PS51323. IGFBP_N_2. 1 hit. PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	607483.

Entry information

Entry name

IBP6_RAT

Accession

Primary (citable) accession number: P35572
Secondary accession number(s): Q499W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 29, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program