Reviewed, UniProtKB/Swiss-Prot P35571 (GPDM_RAT)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase, mitochondrial
      Short name=GPDH-M
      Short name=GPD-M
    EC=1.1.5.3

Gene names

Name:

Gpd2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.
Cofactor	FAD.
Enzyme regulation	Calcium-binding enhance the activity of the enzyme.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Ligand	Calcium FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	NADH metabolic process Inferred from direct assay. Source: RGD glycerol-3-phosphate metabolic process Inferred from direct assay. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from direct assay. Source: RGD glycerol-3-phosphate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 42	42	Mitochondrion Ref.1
	Chain	43 – 727	685	Glycerol-3-phosphate dehydrogenase, mitochondrial		PRO_0000010431
Regions
	Domain	623 – 658	36	EF-hand 1
	Domain	659 – 694	36	EF-hand 2
	Nucleotide binding	71 – 99	29	FAD Potential
	Calcium binding	672 – 683	12	Potential
Amino acid modifications
	Modified residue	600	1	Phosphotyrosine By similarity
	Modified residue	601	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35571-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: B2AAA45E93F5F7CB	FASTA	727	80,973
	10 20 30 40 50 60 MAFQKVVKGT ILMGGGALAT VLGLSQFAHY RRKQVSLAYV EAATCFSEPV NREPPSREAQ 70 80 90 100 110 120 LMTLQNTSEF DILVIGGGAT GCGCALDAVT RGLKTALVER NDFASGTSSR STKLIHGGVR 130 140 150 160 170 180 YLQKAITNLD VEQYRMVKEA LHERANLLEI APHLSAPLPI MLPLYKWWQL PYYWVGIKMY 190 200 210 220 230 240 DLVAGSHCLK SSYVLSKSRA LEHFPMLQKD KLVGAIVYYD GQHNDARMNL AIALTAARYG 250 260 270 280 290 300 AATANYMEVV SLLKKTDPET GKERVSGARC KDVLTGHEFN VRAKCVINAT GPFTDSVRKM 310 320 330 340 350 360 DDNDVVPICQ PSAGVHIVMP GYYSPENMGL LDPATSDGRV IFFLPWEKMT IAGTTDSPTD 370 380 390 400 410 420 VTHHPIPSED DINFILNEVR NYLSCDVEVR RGDVLAAWSG IRPLVTDPKS ANTQSISRNH 430 440 450 460 470 480 VVEVSDSGLI TIAGGKWTTY RSMAEDTVNK AVKLHNLNAG PSRTVGLFLQ GGKDWSPTLY 490 500 510 520 530 540 IRLVQDYGLE SEVAQHLAKT YGDKAFDVAK MASVTGKRWP VVGVRLVSEF PYIEAEVKYG 550 560 570 580 590 600 IKEYACTAVD MISRRTRLAF LNVQAAEEAL PKIVELMGRE LNWSELRKQE ELETATRFLY 610 620 630 640 650 660 YEMGYKSRTE QLTDSTEISL LPPDIDRYKK RFHMFDEDEK GFITIVDVQR VLESINVQMD 670 680 690 700 710 720 EDTLHEILCE VDLNKNGQVE LHEFLQLMSA VHTGRVSGSR LAILMKTAEE NLDRRVPIPV DRSCGGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of rat mitochondrial glycerol-3-phosphate dehydrogenase cDNA. Evidence for EF-hand calcium-binding domains." Brown L.J., McDonald M.J., Lehn D.A., Moran S.M. J. Biol. Chem. 269:14363-14366(1994) [PubMed: 8182039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 43-58. Strain: Sprague-Dawley. Tissue: Testis.
[2]	"Cloning of a cDNA for the FAD-linked glycerol-3-phosphate dehydrogenase from rat liver and its regulation by thyroid hormones." Mueller S., Seitz H.J. Proc. Natl. Acad. Sci. U.S.A. 91:10581-10585(1994) [PubMed: 7937996] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U08027 mRNA. Translation: AAB60443.1. X78593 mRNA. Translation: CAA55329.1. BC083565 mRNA. Translation: AAH83565.1.
IPI	IPI00199663.
PIR	A54051.
RefSeq	NP_036868.1.
UniGene	Rn.89705
3D structure databases
HSSP	HSSP built from PDB template 1CDM based on UniProtKB P02593.
ModBase	Search...
PTM databases
PhosphoSite	P35571.
Proteomic databases
PRIDE	P35571.
Genome annotation databases
GeneID	25062.
KEGG	rno:25062.
Organism-specific databases
RGD	2726. Gpd2.
Phylogenomic databases
HOVERGEN	P35571.
Enzyme and pathway databases
BRENDA	1.1.99.5. 248.
Gene expression databases
ArrayExpress	P35571.
Family and domain databases
InterPro	IPR011992. EF-Hand_type. IPR018248. EF_hand. IPR018247. EF_HAND_1. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR000447. FAD-dep_Gly3P_DH. IPR006076. FAD-dep_OxRdtase. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF01266. DAO. 1 hit. PF00036. efhand. 1 hit. [Graphical view]
PRINTS	PR01001. FADG3PDH.
ProDom	PD000012. EF-hand. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. PS00977. FAD_G3PDH_1. 1 hit. PS00978. FAD_G3PDH_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	605290.

Entry information

Entry name

GPDM_RAT

Accession

Primary (citable) accession number: P35571

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)