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Protein

Glycerol-3-phosphate dehydrogenase, mitochondrial

Gene

Gpd2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

Cofactori

Enzyme regulationi

Calcium-binding enhance the activity of the enzyme.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi71 – 9929FADSequence AnalysisAdd
BLAST
Calcium bindingi672 – 68312PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: RGD
  3. sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycerol-3-phosphate metabolic process Source: RGD
  2. glycerol catabolic process Source: UniProtKB-UniPathway
  3. NADH metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calcium, FAD, Flavoprotein, Metal-binding

Enzyme and pathway databases

BRENDAi1.1.5.3. 5301.
UniPathwayiUPA00618; UER00673.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase, mitochondrial (EC:1.1.5.3)
Short name:
GPD-M
Short name:
GPDH-M
Gene namesi
Name:Gpd2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2726. Gpd2.

Subcellular locationi

GO - Cellular componenti

  1. glycerol-3-phosphate dehydrogenase complex Source: InterPro
  2. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4242Mitochondrion1 PublicationAdd
BLAST
Chaini43 – 727685Glycerol-3-phosphate dehydrogenase, mitochondrialPRO_0000010431Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei601 – 6011PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP35571.
PRIDEiP35571.

2D gel databases

World-2DPAGE0004:P35571.

PTM databases

PhosphoSiteiP35571.

Expressioni

Gene expression databases

GenevestigatoriP35571.

Interactioni

Protein-protein interaction databases

BioGridi247139. 1 interaction.
STRINGi10116.ENSRNOP00000063785.

Structurei

3D structure databases

ProteinModelPortaliP35571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini623 – 65836EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini659 – 69436EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG0578.
HOGENOMiHOG000004813.
HOVERGENiHBG005897.
InParanoidiP35571.
KOiK00111.
PhylomeDBiP35571.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR006076. FAD-dep_OxRdtase.
IPR000447. G3P_DH_FAD-dep.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
PRINTSiPR01001. FADG3PDH.
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35571-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFQKVVKGT ILMGGGALAT VLGLSQFAHY RRKQVSLAYV EAATCFSEPV
60 70 80 90 100
NREPPSREAQ LMTLQNTSEF DILVIGGGAT GCGCALDAVT RGLKTALVER
110 120 130 140 150
NDFASGTSSR STKLIHGGVR YLQKAITNLD VEQYRMVKEA LHERANLLEI
160 170 180 190 200
APHLSAPLPI MLPLYKWWQL PYYWVGIKMY DLVAGSHCLK SSYVLSKSRA
210 220 230 240 250
LEHFPMLQKD KLVGAIVYYD GQHNDARMNL AIALTAARYG AATANYMEVV
260 270 280 290 300
SLLKKTDPET GKERVSGARC KDVLTGHEFN VRAKCVINAT GPFTDSVRKM
310 320 330 340 350
DDNDVVPICQ PSAGVHIVMP GYYSPENMGL LDPATSDGRV IFFLPWEKMT
360 370 380 390 400
IAGTTDSPTD VTHHPIPSED DINFILNEVR NYLSCDVEVR RGDVLAAWSG
410 420 430 440 450
IRPLVTDPKS ANTQSISRNH VVEVSDSGLI TIAGGKWTTY RSMAEDTVNK
460 470 480 490 500
AVKLHNLNAG PSRTVGLFLQ GGKDWSPTLY IRLVQDYGLE SEVAQHLAKT
510 520 530 540 550
YGDKAFDVAK MASVTGKRWP VVGVRLVSEF PYIEAEVKYG IKEYACTAVD
560 570 580 590 600
MISRRTRLAF LNVQAAEEAL PKIVELMGRE LNWSELRKQE ELETATRFLY
610 620 630 640 650
YEMGYKSRTE QLTDSTEISL LPPDIDRYKK RFHMFDEDEK GFITIVDVQR
660 670 680 690 700
VLESINVQMD EDTLHEILCE VDLNKNGQVE LHEFLQLMSA VHTGRVSGSR
710 720
LAILMKTAEE NLDRRVPIPV DRSCGGL
Length:727
Mass (Da):80,973
Last modified:June 1, 1994 - v1
Checksum:iB2AAA45E93F5F7CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08027 mRNA. Translation: AAB60443.1.
X78593 mRNA. Translation: CAA55329.1.
BC083565 mRNA. Translation: AAH83565.1.
PIRiA54051.
RefSeqiNP_036868.1. NM_012736.1.
UniGeneiRn.89705.

Genome annotation databases

GeneIDi25062.
KEGGirno:25062.
UCSCiRGD:2726. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08027 mRNA. Translation: AAB60443.1.
X78593 mRNA. Translation: CAA55329.1.
BC083565 mRNA. Translation: AAH83565.1.
PIRiA54051.
RefSeqiNP_036868.1. NM_012736.1.
UniGeneiRn.89705.

3D structure databases

ProteinModelPortaliP35571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247139. 1 interaction.
STRINGi10116.ENSRNOP00000063785.

PTM databases

PhosphoSiteiP35571.

2D gel databases

World-2DPAGE0004:P35571.

Proteomic databases

PaxDbiP35571.
PRIDEiP35571.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25062.
KEGGirno:25062.
UCSCiRGD:2726. rat.

Organism-specific databases

CTDi2820.
RGDi2726. Gpd2.

Phylogenomic databases

eggNOGiCOG0578.
HOGENOMiHOG000004813.
HOVERGENiHBG005897.
InParanoidiP35571.
KOiK00111.
PhylomeDBiP35571.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00673.
BRENDAi1.1.5.3. 5301.

Miscellaneous databases

NextBioi605290.
PROiP35571.

Gene expression databases

GenevestigatoriP35571.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR006076. FAD-dep_OxRdtase.
IPR000447. G3P_DH_FAD-dep.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
PRINTSiPR01001. FADG3PDH.
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of rat mitochondrial glycerol-3-phosphate dehydrogenase cDNA. Evidence for EF-hand calcium-binding domains."
    Brown L.J., McDonald M.J., Lehn D.A., Moran S.M.
    J. Biol. Chem. 269:14363-14366(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 43-58.
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. "Cloning of a cDNA for the FAD-linked glycerol-3-phosphate dehydrogenase from rat liver and its regulation by thyroid hormones."
    Mueller S., Seitz H.J.
    Proc. Natl. Acad. Sci. U.S.A. 91:10581-10585(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiGPDM_RAT
AccessioniPrimary (citable) accession number: P35571
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 1, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.