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P35571

- GPDM_RAT

UniProt

P35571 - GPDM_RAT

Protein

Glycerol-3-phosphate dehydrogenase, mitochondrial

Gene

Gpd2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

    Cofactori

    FAD.

    Enzyme regulationi

    Calcium-binding enhance the activity of the enzyme.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi71 – 9929FADSequence AnalysisAdd
    BLAST
    Calcium bindingi672 – 68312PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: RGD
    3. sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycerol-3-phosphate metabolic process Source: RGD
    2. glycerol catabolic process Source: UniProtKB-UniPathway
    3. NADH metabolic process Source: RGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Calcium, FAD, Flavoprotein, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00618; UER00673.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate dehydrogenase, mitochondrial (EC:1.1.5.3)
    Short name:
    GPD-M
    Short name:
    GPDH-M
    Gene namesi
    Name:Gpd2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2726. Gpd2.

    Subcellular locationi

    GO - Cellular componenti

    1. glycerol-3-phosphate dehydrogenase complex Source: InterPro
    2. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4242Mitochondrion1 PublicationAdd
    BLAST
    Chaini43 – 727685Glycerol-3-phosphate dehydrogenase, mitochondrialPRO_0000010431Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei601 – 6011PhosphotyrosineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP35571.
    PRIDEiP35571.

    2D gel databases

    World-2DPAGE0004:P35571.

    PTM databases

    PhosphoSiteiP35571.

    Expressioni

    Gene expression databases

    GenevestigatoriP35571.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000063785.

    Structurei

    3D structure databases

    ProteinModelPortaliP35571.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini623 – 65836EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini659 – 69436EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    eggNOGiCOG0578.
    HOGENOMiHOG000004813.
    HOVERGENiHBG005897.
    KOiK00111.
    PhylomeDBiP35571.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR006076. FAD-dep_OxRdtase.
    IPR000447. G3P_DH_FAD-dep.
    [Graphical view]
    PfamiPF01266. DAO. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view]
    PRINTSiPR01001. FADG3PDH.
    SMARTiSM00054. EFh. 2 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    PS00977. FAD_G3PDH_1. 1 hit.
    PS00978. FAD_G3PDH_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35571-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFQKVVKGT ILMGGGALAT VLGLSQFAHY RRKQVSLAYV EAATCFSEPV    50
    NREPPSREAQ LMTLQNTSEF DILVIGGGAT GCGCALDAVT RGLKTALVER 100
    NDFASGTSSR STKLIHGGVR YLQKAITNLD VEQYRMVKEA LHERANLLEI 150
    APHLSAPLPI MLPLYKWWQL PYYWVGIKMY DLVAGSHCLK SSYVLSKSRA 200
    LEHFPMLQKD KLVGAIVYYD GQHNDARMNL AIALTAARYG AATANYMEVV 250
    SLLKKTDPET GKERVSGARC KDVLTGHEFN VRAKCVINAT GPFTDSVRKM 300
    DDNDVVPICQ PSAGVHIVMP GYYSPENMGL LDPATSDGRV IFFLPWEKMT 350
    IAGTTDSPTD VTHHPIPSED DINFILNEVR NYLSCDVEVR RGDVLAAWSG 400
    IRPLVTDPKS ANTQSISRNH VVEVSDSGLI TIAGGKWTTY RSMAEDTVNK 450
    AVKLHNLNAG PSRTVGLFLQ GGKDWSPTLY IRLVQDYGLE SEVAQHLAKT 500
    YGDKAFDVAK MASVTGKRWP VVGVRLVSEF PYIEAEVKYG IKEYACTAVD 550
    MISRRTRLAF LNVQAAEEAL PKIVELMGRE LNWSELRKQE ELETATRFLY 600
    YEMGYKSRTE QLTDSTEISL LPPDIDRYKK RFHMFDEDEK GFITIVDVQR 650
    VLESINVQMD EDTLHEILCE VDLNKNGQVE LHEFLQLMSA VHTGRVSGSR 700
    LAILMKTAEE NLDRRVPIPV DRSCGGL 727
    Length:727
    Mass (Da):80,973
    Last modified:June 1, 1994 - v1
    Checksum:iB2AAA45E93F5F7CB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08027 mRNA. Translation: AAB60443.1.
    X78593 mRNA. Translation: CAA55329.1.
    BC083565 mRNA. Translation: AAH83565.1.
    PIRiA54051.
    RefSeqiNP_036868.1. NM_012736.1.
    UniGeneiRn.89705.

    Genome annotation databases

    GeneIDi25062.
    KEGGirno:25062.
    UCSCiRGD:2726. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08027 mRNA. Translation: AAB60443.1 .
    X78593 mRNA. Translation: CAA55329.1 .
    BC083565 mRNA. Translation: AAH83565.1 .
    PIRi A54051.
    RefSeqi NP_036868.1. NM_012736.1.
    UniGenei Rn.89705.

    3D structure databases

    ProteinModelPortali P35571.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000063785.

    PTM databases

    PhosphoSitei P35571.

    2D gel databases

    World-2DPAGE 0004:P35571.

    Proteomic databases

    PaxDbi P35571.
    PRIDEi P35571.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 25062.
    KEGGi rno:25062.
    UCSCi RGD:2726. rat.

    Organism-specific databases

    CTDi 2820.
    RGDi 2726. Gpd2.

    Phylogenomic databases

    eggNOGi COG0578.
    HOGENOMi HOG000004813.
    HOVERGENi HBG005897.
    KOi K00111.
    PhylomeDBi P35571.

    Enzyme and pathway databases

    UniPathwayi UPA00618 ; UER00673 .

    Miscellaneous databases

    NextBioi 605290.
    PROi P35571.

    Gene expression databases

    Genevestigatori P35571.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR006076. FAD-dep_OxRdtase.
    IPR000447. G3P_DH_FAD-dep.
    [Graphical view ]
    Pfami PF01266. DAO. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    PRINTSi PR01001. FADG3PDH.
    SMARTi SM00054. EFh. 2 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    PS00977. FAD_G3PDH_1. 1 hit.
    PS00978. FAD_G3PDH_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of rat mitochondrial glycerol-3-phosphate dehydrogenase cDNA. Evidence for EF-hand calcium-binding domains."
      Brown L.J., McDonald M.J., Lehn D.A., Moran S.M.
      J. Biol. Chem. 269:14363-14366(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 43-58.
      Strain: Sprague-Dawley.
      Tissue: Testis.
    2. "Cloning of a cDNA for the FAD-linked glycerol-3-phosphate dehydrogenase from rat liver and its regulation by thyroid hormones."
      Mueller S., Seitz H.J.
      Proc. Natl. Acad. Sci. U.S.A. 91:10581-10585(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiGPDM_RAT
    AccessioniPrimary (citable) accession number: P35571
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3