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Protein

Glycerol-3-phosphate dehydrogenase, mitochondrial

Gene

Gpd2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

Cofactori

Enzyme regulationi

Calcium-binding enhance the activity of the enzyme.

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route).
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol-3-phosphate dehydrogenase, mitochondrial (Gpd2)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route), the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi71 – 99FADSequence analysisAdd BLAST29
Calcium bindingi672 – 683PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandCalcium, FAD, Flavoprotein, Metal-binding

Enzyme and pathway databases

BRENDAi1.1.5.3 5301
UniPathwayiUPA00618; UER00673

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase, mitochondrial (EC:1.1.5.3)
Short name:
GPD-M
Short name:
GPDH-M
Gene namesi
Name:Gpd2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2726 Gpd2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42Mitochondrion1 PublicationAdd BLAST42
ChainiPRO_000001043143 – 727Glycerol-3-phosphate dehydrogenase, mitochondrialAdd BLAST685

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei601PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP35571
PRIDEiP35571

2D gel databases

World-2DPAGEi0004:P35571

PTM databases

iPTMnetiP35571
PhosphoSitePlusiP35571

Interactioni

Protein-protein interaction databases

BioGridi247139, 1 interactor
STRINGi10116.ENSRNOP00000043749

Structurei

3D structure databases

ProteinModelPortaliP35571
SMRiP35571
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini623 – 658EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini659 – 694EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0042 Eukaryota
COG0578 LUCA
HOGENOMiHOG000004813
HOVERGENiHBG005897
InParanoidiP35571
KOiK00111
PhylomeDBiP35571

Family and domain databases

CDDicd00051 EFh, 1 hit
Gene3Di1.10.8.870, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR031656 DAO_C
IPR038299 DAO_C_sf
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR006076 FAD-dep_OxRdtase
IPR036188 FAD/NAD-bd_sf
IPR000447 G3P_DH_FAD-dep
PANTHERiPTHR11985 PTHR11985, 1 hit
PfamiView protein in Pfam
PF01266 DAO, 1 hit
PF16901 DAO_C, 1 hit
PF13499 EF-hand_7, 1 hit
PRINTSiPR01001 FADG3PDH
SMARTiView protein in SMART
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51905 SSF51905, 2 hits
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 2 hits
PS00977 FAD_G3PDH_1, 1 hit
PS00978 FAD_G3PDH_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35571-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFQKVVKGT ILMGGGALAT VLGLSQFAHY RRKQVSLAYV EAATCFSEPV
60 70 80 90 100
NREPPSREAQ LMTLQNTSEF DILVIGGGAT GCGCALDAVT RGLKTALVER
110 120 130 140 150
NDFASGTSSR STKLIHGGVR YLQKAITNLD VEQYRMVKEA LHERANLLEI
160 170 180 190 200
APHLSAPLPI MLPLYKWWQL PYYWVGIKMY DLVAGSHCLK SSYVLSKSRA
210 220 230 240 250
LEHFPMLQKD KLVGAIVYYD GQHNDARMNL AIALTAARYG AATANYMEVV
260 270 280 290 300
SLLKKTDPET GKERVSGARC KDVLTGHEFN VRAKCVINAT GPFTDSVRKM
310 320 330 340 350
DDNDVVPICQ PSAGVHIVMP GYYSPENMGL LDPATSDGRV IFFLPWEKMT
360 370 380 390 400
IAGTTDSPTD VTHHPIPSED DINFILNEVR NYLSCDVEVR RGDVLAAWSG
410 420 430 440 450
IRPLVTDPKS ANTQSISRNH VVEVSDSGLI TIAGGKWTTY RSMAEDTVNK
460 470 480 490 500
AVKLHNLNAG PSRTVGLFLQ GGKDWSPTLY IRLVQDYGLE SEVAQHLAKT
510 520 530 540 550
YGDKAFDVAK MASVTGKRWP VVGVRLVSEF PYIEAEVKYG IKEYACTAVD
560 570 580 590 600
MISRRTRLAF LNVQAAEEAL PKIVELMGRE LNWSELRKQE ELETATRFLY
610 620 630 640 650
YEMGYKSRTE QLTDSTEISL LPPDIDRYKK RFHMFDEDEK GFITIVDVQR
660 670 680 690 700
VLESINVQMD EDTLHEILCE VDLNKNGQVE LHEFLQLMSA VHTGRVSGSR
710 720
LAILMKTAEE NLDRRVPIPV DRSCGGL
Length:727
Mass (Da):80,973
Last modified:June 1, 1994 - v1
Checksum:iB2AAA45E93F5F7CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08027 mRNA Translation: AAB60443.1
X78593 mRNA Translation: CAA55329.1
BC083565 mRNA Translation: AAH83565.1
PIRiA54051
RefSeqiNP_036868.1, NM_012736.1
UniGeneiRn.89705

Genome annotation databases

GeneIDi25062
KEGGirno:25062
UCSCiRGD:2726 rat

Similar proteinsi

Entry informationi

Entry nameiGPDM_RAT
AccessioniPrimary (citable) accession number: P35571
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health