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P35571 (GPDM_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate dehydrogenase, mitochondrial

Short name=GPD-M
Short name=GPDH-M
EC=1.1.5.3
Gene names
Name:Gpd2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length727 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

Cofactor

FAD.

Enzyme regulation

Calcium-binding enhance the activity of the enzyme.

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1.

Subcellular location

Mitochondrion.

Sequence similarities

Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

Contains 2 EF-hand domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4242Mitochondrion Ref.1
Chain43 – 727685Glycerol-3-phosphate dehydrogenase, mitochondrial
PRO_0000010431

Regions

Domain623 – 65836EF-hand 1
Domain659 – 69436EF-hand 2
Nucleotide binding71 – 9929FAD Potential
Calcium binding672 – 68312 Potential

Amino acid modifications

Modified residue6011Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
P35571 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: B2AAA45E93F5F7CB

FASTA72780,973
        10         20         30         40         50         60 
MAFQKVVKGT ILMGGGALAT VLGLSQFAHY RRKQVSLAYV EAATCFSEPV NREPPSREAQ 

        70         80         90        100        110        120 
LMTLQNTSEF DILVIGGGAT GCGCALDAVT RGLKTALVER NDFASGTSSR STKLIHGGVR 

       130        140        150        160        170        180 
YLQKAITNLD VEQYRMVKEA LHERANLLEI APHLSAPLPI MLPLYKWWQL PYYWVGIKMY 

       190        200        210        220        230        240 
DLVAGSHCLK SSYVLSKSRA LEHFPMLQKD KLVGAIVYYD GQHNDARMNL AIALTAARYG 

       250        260        270        280        290        300 
AATANYMEVV SLLKKTDPET GKERVSGARC KDVLTGHEFN VRAKCVINAT GPFTDSVRKM 

       310        320        330        340        350        360 
DDNDVVPICQ PSAGVHIVMP GYYSPENMGL LDPATSDGRV IFFLPWEKMT IAGTTDSPTD 

       370        380        390        400        410        420 
VTHHPIPSED DINFILNEVR NYLSCDVEVR RGDVLAAWSG IRPLVTDPKS ANTQSISRNH 

       430        440        450        460        470        480 
VVEVSDSGLI TIAGGKWTTY RSMAEDTVNK AVKLHNLNAG PSRTVGLFLQ GGKDWSPTLY 

       490        500        510        520        530        540 
IRLVQDYGLE SEVAQHLAKT YGDKAFDVAK MASVTGKRWP VVGVRLVSEF PYIEAEVKYG 

       550        560        570        580        590        600 
IKEYACTAVD MISRRTRLAF LNVQAAEEAL PKIVELMGRE LNWSELRKQE ELETATRFLY 

       610        620        630        640        650        660 
YEMGYKSRTE QLTDSTEISL LPPDIDRYKK RFHMFDEDEK GFITIVDVQR VLESINVQMD 

       670        680        690        700        710        720 
EDTLHEILCE VDLNKNGQVE LHEFLQLMSA VHTGRVSGSR LAILMKTAEE NLDRRVPIPV 


DRSCGGL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of rat mitochondrial glycerol-3-phosphate dehydrogenase cDNA. Evidence for EF-hand calcium-binding domains."
Brown L.J., McDonald M.J., Lehn D.A., Moran S.M.
J. Biol. Chem. 269:14363-14366(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 43-58.
Strain: Sprague-Dawley.
Tissue: Testis.
[2]"Cloning of a cDNA for the FAD-linked glycerol-3-phosphate dehydrogenase from rat liver and its regulation by thyroid hormones."
Mueller S., Seitz H.J.
Proc. Natl. Acad. Sci. U.S.A. 91:10581-10585(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U08027 mRNA. Translation: AAB60443.1.
X78593 mRNA. Translation: CAA55329.1.
BC083565 mRNA. Translation: AAH83565.1.
PIRA54051.
RefSeqNP_036868.1. NM_012736.1.
UniGeneRn.89705.

3D structure databases

ProteinModelPortalP35571.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000063785.

PTM databases

PhosphoSiteP35571.

2D gel databases

World-2DPAGE0004:P35571.

Proteomic databases

PaxDbP35571.
PRIDEP35571.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID25062.
KEGGrno:25062.
UCSCRGD:2726. rat.

Organism-specific databases

CTD2820.
RGD2726. Gpd2.

Phylogenomic databases

eggNOGCOG0578.
HOGENOMHOG000004813.
HOVERGENHBG005897.
KOK00111.
PhylomeDBP35571.

Enzyme and pathway databases

UniPathwayUPA00618; UER00673.

Gene expression databases

GenevestigatorP35571.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR006076. FAD-dep_OxRdtase.
IPR000447. G3P_DH_FAD-dep.
[Graphical view]
PfamPF01266. DAO. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
PRINTSPR01001. FADG3PDH.
SMARTSM00054. EFh. 2 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio605290.
PROP35571.

Entry information

Entry nameGPDM_RAT
AccessionPrimary (citable) accession number: P35571
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways