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P35567 (CDK1A_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 1-A

Short name=CDK1-A
EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cell division control protein 2 homolog 1
Cell division control protein 2-A
Cell division protein kinase 1-A
p34 protein kinase 1
Gene names
Name:cdk1-a
Synonyms:cdc2-a, cdc2x1.1
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulation

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it. Ref.1

Subunit structure

Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with spdya. Ref.1 Ref.6 Ref.7

Subcellular location

Nucleus By similarity.

Post-translational modification

Phosphorylation at Tyr-15 by wee1 and wee2 inhibits the protein kinase activity and acts negative regulator of entry into mitosis (G2 to M transition).

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 302302Cyclin-dependent kinase 1-A
PRO_0000085735

Regions

Domain4 – 287284Protein kinase
Nucleotide binding10 – 189ATP By similarity

Sites

Active site1281Proton acceptor By similarity
Binding site331ATP By similarity

Amino acid modifications

Modified residue141Phosphothreonine By similarity
Modified residue151Phosphotyrosine; by wee1 and wee2 Ref.4 Ref.5
Modified residue1611Phosphothreonine; by CAK Ref.3
Modified residue2771Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P35567 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 90D2113216DF31DA

FASTA30234,506
        10         20         30         40         50         60 
MDEYTKIEKI GEGTYGVVYK GRHKATGQVV AMKKIRLENE EEGVPSTAIR EISLLKELQH 

        70         80         90        100        110        120 
PNIVCLLDVL MQDSRLYLIF EFLSMDLKKY LDSIPSGQYI DTMLVKSYLY QILQGIVFCH 

       130        140        150        160        170        180 
SRRVLHRDLK PQNLLIDSKG VIKLADFGLA RAFGIPVRVY THEVVTLWYR APEVLLGSVR 

       190        200        210        220        230        240 
YSTPVDVWSI GTIFAEIATK KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNS 

       250        260        270        280        290        300 
FPKWKGGSLS ANVKNIDKDG LDLLAKMLIY DPAKRISARK ALLHPYFDDL DKSSLPDNQI 


RN 

« Hide

References

« Hide 'large scale' references
[1]"Requirement of mosXe protein kinase for meiotic maturation of Xenopus oocytes induced by a cdc2 mutant lacking regulatory phosphorylation sites."
Pickham K.M., Meyer A.N., Li J., Donoghue D.J.
Mol. Cell. Biol. 12:3192-3203(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION, SUBUNIT.
Tissue: Oocyte.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.
[3]"The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues."
Fesquet D., Labbe J.-C., Derancourt J., Capony J.-P., Galas S., Girard F., Lorca T., Shuttleworth J., Doree M., Cavadore J.-C.
EMBO J. 12:3111-3121(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT THR-161.
[4]"Cell cycle regulation of a Xenopus Wee1-like kinase."
Mueller P.R., Coleman T.R., Dunphy W.G.
Mol. Biol. Cell 6:119-134(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT TYR-15.
[5]"Analysis of the early embryonic cell cycles of Xenopus; regulation of cell cycle length by Xe-wee1 and Mos."
Murakami M.S., Vande Woude G.F.
Development 125:237-248(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT TYR-15.
[6]"A novel p34cdc2 binding and activating protein that is necessary and sufficient to trigger G2/M progression in Xenopus oocytes."
Ferby I., Blazquez M., Palmer A., Eritja R., Nebreda A.R.
Genes Dev. 13:2177-2189(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SPDYA.
[7]"Identification and comparative analysis of multiple mammalian Speedy/Ringo proteins."
Cheng A., Xiong W., Ferrell J.E. Jr., Solomon M.J.
Cell Cycle 4:155-165(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SPDYA.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60680 mRNA. Translation: AAA63561.1.
BC045078 mRNA. Translation: AAH45078.1.
PIRA44349.
RefSeqNP_001080554.1. NM_001087085.1.
UniGeneXl.8917.

3D structure databases

ProteinModelPortalP35567.
SMRP35567. Positions 1-292.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid98488. 13 interactions.
MINTMINT-102773.

Proteomic databases

PRIDEP35567.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID380246.
KEGGxla:380246.

Organism-specific databases

CTD380246.
XenbaseXB-GENE-482754. cdk1.

Phylogenomic databases

HOVERGENHBG014652.
KOK02087.

Enzyme and pathway databases

BRENDA2.7.11.22. 6726.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDK1A_XENLA
AccessionPrimary (citable) accession number: P35567
Secondary accession number(s): Q5D0B3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families