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P35567

- CDK1A_XENLA

UniProt

P35567 - CDK1A_XENLA

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Protein

Cyclin-dependent kinase 1-A

Gene

cdk1-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Active sitei128 – 1281Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
  3. protein serine/threonine kinase activity Source: UniProtKB
  4. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
  2. regulation of cell cycle Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 6726.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1-A (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDK1-A
Alternative name(s):
Cell division control protein 2 homolog 1
Cell division control protein 2-A
Cell division protein kinase 1-A
p34 protein kinase 1
Gene namesi
Name:cdk1-a
Synonyms:cdc2-a, cdc2x1.1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-482754. cdk1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Cyclin-dependent kinase 1-APRO_0000085735Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphothreonineBy similarity
Modified residuei15 – 151Phosphotyrosine; by wee1 and wee22 Publications
Modified residuei161 – 1611Phosphothreonine; by CAK1 Publication
Modified residuei277 – 2771PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation at Tyr-15 by wee1 and wee2 inhibits the protein kinase activity and acts negative regulator of entry into mitosis (G2 to M transition).3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP35567.

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with spdya.3 Publications

Protein-protein interaction databases

BioGridi98488. 14 interactions.
MINTiMINT-102773.

Structurei

3D structure databases

ProteinModelPortaliP35567.
SMRiP35567. Positions 1-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 287284Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG014652.
KOiK02087.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35567-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDEYTKIEKI GEGTYGVVYK GRHKATGQVV AMKKIRLENE EEGVPSTAIR
60 70 80 90 100
EISLLKELQH PNIVCLLDVL MQDSRLYLIF EFLSMDLKKY LDSIPSGQYI
110 120 130 140 150
DTMLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDSKG VIKLADFGLA
160 170 180 190 200
RAFGIPVRVY THEVVTLWYR APEVLLGSVR YSTPVDVWSI GTIFAEIATK
210 220 230 240 250
KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNS FPKWKGGSLS
260 270 280 290 300
ANVKNIDKDG LDLLAKMLIY DPAKRISARK ALLHPYFDDL DKSSLPDNQI

RN
Length:302
Mass (Da):34,506
Last modified:June 1, 1994 - v1
Checksum:i90D2113216DF31DA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60680 mRNA. Translation: AAA63561.1.
BC045078 mRNA. Translation: AAH45078.1.
PIRiA44349.
RefSeqiNP_001080554.1. NM_001087085.1.
UniGeneiXl.8917.

Genome annotation databases

GeneIDi380246.
KEGGixla:380246.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60680 mRNA. Translation: AAA63561.1 .
BC045078 mRNA. Translation: AAH45078.1 .
PIRi A44349.
RefSeqi NP_001080554.1. NM_001087085.1.
UniGenei Xl.8917.

3D structure databases

ProteinModelPortali P35567.
SMRi P35567. Positions 1-292.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 98488. 14 interactions.
MINTi MINT-102773.

Proteomic databases

PRIDEi P35567.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 380246.
KEGGi xla:380246.

Organism-specific databases

CTDi 380246.
Xenbasei XB-GENE-482754. cdk1.

Phylogenomic databases

HOVERGENi HBG014652.
KOi K02087.

Enzyme and pathway databases

BRENDAi 2.7.11.22. 6726.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Requirement of mosXe protein kinase for meiotic maturation of Xenopus oocytes induced by a cdc2 mutant lacking regulatory phosphorylation sites."
    Pickham K.M., Meyer A.N., Li J., Donoghue D.J.
    Mol. Cell. Biol. 12:3192-3203(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION, SUBUNIT.
    Tissue: Oocyte.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.
  3. "The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues."
    Fesquet D., Labbe J.-C., Derancourt J., Capony J.-P., Galas S., Girard F., Lorca T., Shuttleworth J., Doree M., Cavadore J.-C.
    EMBO J. 12:3111-3121(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-161.
  4. "Cell cycle regulation of a Xenopus Wee1-like kinase."
    Mueller P.R., Coleman T.R., Dunphy W.G.
    Mol. Biol. Cell 6:119-134(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT TYR-15.
  5. "Analysis of the early embryonic cell cycles of Xenopus; regulation of cell cycle length by Xe-wee1 and Mos."
    Murakami M.S., Vande Woude G.F.
    Development 125:237-248(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT TYR-15.
  6. "A novel p34cdc2 binding and activating protein that is necessary and sufficient to trigger G2/M progression in Xenopus oocytes."
    Ferby I., Blazquez M., Palmer A., Eritja R., Nebreda A.R.
    Genes Dev. 13:2177-2189(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPDYA.
  7. "Identification and comparative analysis of multiple mammalian Speedy/Ringo proteins."
    Cheng A., Xiong W., Ferrell J.E. Jr., Solomon M.J.
    Cell Cycle 4:155-165(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPDYA.

Entry informationi

Entry nameiCDK1A_XENLA
AccessioniPrimary (citable) accession number: P35567
Secondary accession number(s): Q5D0B3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3