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Protein

Calnexin

Gene

Canx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi75Calcium; via carbonyl oxygenBy similarity1
Metal bindingi118Calcium; via carbonyl oxygenBy similarity1
Binding sitei165CarbohydrateBy similarity1
Binding sitei167CarbohydrateBy similarity1
Binding sitei186CarbohydrateBy similarity1
Binding sitei217CarbohydrateBy similarity1
Metal bindingi437CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • antigen processing and presentation of peptide antigen via MHC class I Source: Reactome
  • chaperone-mediated protein folding Source: Ensembl
  • clathrin-dependent endocytosis Source: UniProtKB
  • synaptic vesicle endocytosis Source: UniProtKB

Keywordsi

Molecular functionChaperone
LigandCalcium, Lectin, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-447115. Interleukin-12 family signaling.
R-MMU-901042. Calnexin/calreticulin cycle.
R-MMU-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Names & Taxonomyi

Protein namesi
Recommended name:
Calnexin
Gene namesi
Name:Canx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:88261. Canx.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 482LumenalSequence analysisAdd BLAST462
Transmembranei483 – 503HelicalSequence analysisAdd BLAST21
Topological domaini504 – 591CytoplasmicSequence analysisAdd BLAST88

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000000419921 – 591CalnexinAdd BLAST571

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei138N6-acetyllysineBy similarity1
Disulfide bondi161 ↔ 195By similarity
Disulfide bondi361 ↔ 367By similarity
Lipidationi503S-palmitoyl cysteineBy similarity1
Lipidationi504S-palmitoyl cysteineBy similarity1
Modified residuei553PhosphoserineCombined sources1
Modified residuei561PhosphothreonineBy similarity1
Modified residuei563Phosphoserine; by MAPK3Combined sources1
Modified residuei582PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-563 by MAPK3/ERK1. phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity).By similarity
Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins (By similarity).By similarity
Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4.By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP35564.
PaxDbiP35564.
PeptideAtlasiP35564.
PRIDEiP35564.

PTM databases

iPTMnetiP35564.
PhosphoSitePlusiP35564.
SwissPalmiP35564.

Miscellaneous databases

PMAP-CutDBiP35564.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020368.
CleanExiMM_CANX.
ExpressionAtlasiP35564. baseline and differential.
GenevisibleiP35564. MM.

Interactioni

Subunit structurei

Interacts with MAPK3/ERK1 (By similarity). Interacts with KCNH2 (By similarity). Associates with ribosomes (By similarity). The palmitoylated form interacts with the ribosome-translocon complex component SSR1, promoting efficient folding of glycoproteins (By similarity). Interacts with SERPINA2P/SERPINA2 and with the S and Z variants of SERPINA1 (By similarity). Interacts with SGIP1; involved in negative regulation of endocytosis (PubMed:21747946). Interacts with PPIB (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Sgip1Q8VD373EBI-738422,EBI-776269

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198467. 10 interactors.
CORUMiP35564.
IntActiP35564. 17 interactors.
MINTiMINT-1862151.
STRINGi10090.ENSMUSP00000020637.

Structurei

3D structure databases

ProteinModelPortaliP35564.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati279 – 2911-1Add BLAST13
Repeati296 – 3081-2Add BLAST13
Repeati315 – 3271-3Add BLAST13
Repeati334 – 3461-4Add BLAST13
Repeati349 – 3592-1Add BLAST11
Repeati368 – 3782-2Add BLAST11
Repeati382 – 3922-3Add BLAST11
Repeati396 – 4062-4Add BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni277 – 410P domain (Extended arm)By similarityAdd BLAST134
Regioni279 – 3464 X approximate repeatsAdd BLAST68
Regioni327 – 360Interaction with PPIBBy similarityAdd BLAST34
Regioni349 – 4064 X approximate repeatsAdd BLAST58
Regioni504 – 591Sufficient to mediate interaction with SGIP11 PublicationAdd BLAST88

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0675. Eukaryota.
ENOG410XP7T. LUCA.
GeneTreeiENSGT00430000030841.
HOGENOMiHOG000192436.
HOVERGENiHBG005407.
InParanoidiP35564.
KOiK08054.
OMAiEEWAYVG.
OrthoDBiEOG091G04SS.
PhylomeDBiP35564.
TreeFamiTF300618.

Family and domain databases

InterProiView protein in InterPro
IPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiView protein in Pfam
PF00262. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF63887. SSF63887. 1 hit.
PROSITEiView protein in PROSITE
PS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35564-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGKWLLCLL LVLGTAAVEA HDGHDDDAID IEDDLDDVIE EVEDSKSKSD
60 70 80 90 100
ASTPPSPKVT YKAPVPTGEV YFADSFDRGS LSGWILSKAK KDDTDDEIAK
110 120 130 140 150
YDGKWEVDEM KETKLPGDKG LVLMSRAKHH AISAKLNKPF LFDTKPLIVQ
160 170 180 190 200
YEVNFQNGIE CGGAYVKLLS KTAELSLDQF HDKTPYTIMF GPDKCGEDYK
210 220 230 240 250
LHFIFRHKNP KTGVYEEKHA KRPDADLKTY FTDKKTHLYT LILNPDNSFE
260 270 280 290 300
ILVDQSVVNS GNLLNDMTPP VNPSREIEDP EDRKPEDWDE RPKIADPDAV
310 320 330 340 350
KPDDWDEDAP SKIPDEEATK PEGWLDDEPE YIPDPDAEKP EDWDEDMDGE
360 370 380 390 400
WEAPQIANPK CESAPGCGVW QRPMIDNPNY KGKWKPPMID NPNYQGIWKP
410 420 430 440 450
RKIPNPDFFE DLEPFKMTPF SAIGLELWSM TSDIFFDNFI ISGDRRVVDD
460 470 480 490 500
WANDGWGLKK AADGAAEPGV VLQMLEAAEE RPWLWVVYIL TVALPVFLVI
510 520 530 540 550
LFCCSGKKQS NAMEYKKTDA PQPDVKDEEG KEEEKNKRDE EEEEEKLEEK
560 570 580 590
QKSDAEEDGV TGSQDEEDSK PKAEEDEILN RSPRNRKPRR E
Length:591
Mass (Da):67,278
Last modified:June 1, 1994 - v1
Checksum:i0D9F8FE03434BADC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti416K → R in AAA62450 (PubMed:8148318).Curated1
Sequence conflicti468P → L in AAA62450 (PubMed:8148318).Curated1
Sequence conflicti472L → G in AAA62450 (PubMed:8148318).Curated1
Sequence conflicti538R → G in AAA62450 (PubMed:8148318).Curated1
Sequence conflicti560V → G in AAA62450 (PubMed:8148318).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L18888 mRNA. Translation: AAA21014.1.
BC012408 mRNA. Translation: AAH12408.1.
BC040244 mRNA. Translation: AAH40244.1.
AK084175 mRNA. Translation: BAC39133.1.
L23865 mRNA. Translation: AAA62450.1.
CCDSiCCDS24633.1.
PIRiB54354.
RefSeqiNP_001103969.1. NM_001110499.1.
NP_001103970.1. NM_001110500.1.
NP_031623.1. NM_007597.3.
UniGeneiMm.248827.

Genome annotation databases

EnsembliENSMUST00000020637; ENSMUSP00000020637; ENSMUSG00000020368.
ENSMUST00000179865; ENSMUSP00000137440; ENSMUSG00000020368.
GeneIDi12330.
KEGGimmu:12330.
UCSCiuc007isf.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiCALX_MOUSE
AccessioniPrimary (citable) accession number: P35564
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 27, 2017
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families