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Protein

Calnexin

Gene

Canx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi75Calcium; via carbonyl oxygenBy similarity1
Metal bindingi118Calcium; via carbonyl oxygenBy similarity1
Binding sitei165CarbohydrateBy similarity1
Binding sitei167CarbohydrateBy similarity1
Binding sitei186CarbohydrateBy similarity1
Binding sitei217CarbohydrateBy similarity1
Metal bindingi437CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • chaperone-mediated protein folding Source: Ensembl
  • clathrin-dependent endocytosis Source: UniProtKB
  • synaptic vesicle endocytosis Source: UniProtKB

Keywordsi

Molecular functionChaperone
LigandCalcium, Lectin, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2132295 MHC class II antigen presentation
R-MMU-8984722 Interleukin-35 Signalling
R-MMU-901042 Calnexin/calreticulin cycle
R-MMU-9020956 Interleukin-27 signaling
R-MMU-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Names & Taxonomyi

Protein namesi
Recommended name:
Calnexin
Gene namesi
Name:Canx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:88261 Canx

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 482LumenalSequence analysisAdd BLAST462
Transmembranei483 – 503HelicalSequence analysisAdd BLAST21
Topological domaini504 – 591CytoplasmicSequence analysisAdd BLAST88

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000000419921 – 591CalnexinAdd BLAST571

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei138N6-acetyllysineBy similarity1
Disulfide bondi161 ↔ 195By similarity
Disulfide bondi361 ↔ 367By similarity
Lipidationi503S-palmitoyl cysteineBy similarity1
Lipidationi504S-palmitoyl cysteineBy similarity1
Modified residuei553PhosphoserineCombined sources1
Modified residuei561PhosphothreonineBy similarity1
Modified residuei563Phosphoserine; by MAPK3Combined sources1
Modified residuei582PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-563 by MAPK3/ERK1. phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity).By similarity
Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins (By similarity).By similarity
Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4.By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP35564
PaxDbiP35564
PeptideAtlasiP35564
PRIDEiP35564

PTM databases

iPTMnetiP35564
PhosphoSitePlusiP35564
SwissPalmiP35564

Miscellaneous databases

PMAP-CutDBiP35564

Expressioni

Gene expression databases

BgeeiENSMUSG00000020368
CleanExiMM_CANX
ExpressionAtlasiP35564 baseline and differential
GenevisibleiP35564 MM

Interactioni

Subunit structurei

Interacts with MAPK3/ERK1 (By similarity). Interacts with KCNH2 (By similarity). Associates with ribosomes (By similarity). The palmitoylated form interacts with the ribosome-translocon complex component SSR1, promoting efficient folding of glycoproteins (By similarity). Interacts with SERPINA2P/SERPINA2 and with the S and Z variants of SERPINA1 (By similarity). Interacts with SGIP1; involved in negative regulation of endocytosis (PubMed:21747946). Interacts with PPIB (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Sgip1Q8VD373EBI-738422,EBI-776269

GO - Molecular functioni

Protein-protein interaction databases

BioGridi19846712 interactors.
CORUMiP35564
IntActiP35564 18 interactors.
MINTiP35564
STRINGi10090.ENSMUSP00000020637

Structurei

3D structure databases

ProteinModelPortaliP35564
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati279 – 2911-1Add BLAST13
Repeati296 – 3081-2Add BLAST13
Repeati315 – 3271-3Add BLAST13
Repeati334 – 3461-4Add BLAST13
Repeati349 – 3592-1Add BLAST11
Repeati368 – 3782-2Add BLAST11
Repeati382 – 3922-3Add BLAST11
Repeati396 – 4062-4Add BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni277 – 410P domain (Extended arm)By similarityAdd BLAST134
Regioni279 – 3464 X approximate repeatsAdd BLAST68
Regioni327 – 360Interaction with PPIBBy similarityAdd BLAST34
Regioni349 – 4064 X approximate repeatsAdd BLAST58
Regioni504 – 591Sufficient to mediate interaction with SGIP11 PublicationAdd BLAST88

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0675 Eukaryota
ENOG410XP7T LUCA
GeneTreeiENSGT00430000030841
HOGENOMiHOG000192436
HOVERGENiHBG005407
InParanoidiP35564
KOiK08054
OMAiNHKNPKT
OrthoDBiEOG091G04SS
PhylomeDBiP35564
TreeFamiTF300618

Family and domain databases

Gene3Di2.10.250.101 hit
InterProiView protein in InterPro
IPR001580 Calret/calnex
IPR018124 Calret/calnex_CS
IPR009033 Calreticulin/calnexin_P_dom_sf
IPR013320 ConA-like_dom_sf
PANTHERiPTHR11073 PTHR11073, 1 hit
PfamiView protein in Pfam
PF00262 Calreticulin, 1 hit
PRINTSiPR00626 CALRETICULIN
SUPFAMiSSF49899 SSF49899, 2 hits
SSF63887 SSF63887, 1 hit
PROSITEiView protein in PROSITE
PS00803 CALRETICULIN_1, 1 hit
PS00804 CALRETICULIN_2, 1 hit
PS00805 CALRETICULIN_REPEAT, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35564-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGKWLLCLL LVLGTAAVEA HDGHDDDAID IEDDLDDVIE EVEDSKSKSD
60 70 80 90 100
ASTPPSPKVT YKAPVPTGEV YFADSFDRGS LSGWILSKAK KDDTDDEIAK
110 120 130 140 150
YDGKWEVDEM KETKLPGDKG LVLMSRAKHH AISAKLNKPF LFDTKPLIVQ
160 170 180 190 200
YEVNFQNGIE CGGAYVKLLS KTAELSLDQF HDKTPYTIMF GPDKCGEDYK
210 220 230 240 250
LHFIFRHKNP KTGVYEEKHA KRPDADLKTY FTDKKTHLYT LILNPDNSFE
260 270 280 290 300
ILVDQSVVNS GNLLNDMTPP VNPSREIEDP EDRKPEDWDE RPKIADPDAV
310 320 330 340 350
KPDDWDEDAP SKIPDEEATK PEGWLDDEPE YIPDPDAEKP EDWDEDMDGE
360 370 380 390 400
WEAPQIANPK CESAPGCGVW QRPMIDNPNY KGKWKPPMID NPNYQGIWKP
410 420 430 440 450
RKIPNPDFFE DLEPFKMTPF SAIGLELWSM TSDIFFDNFI ISGDRRVVDD
460 470 480 490 500
WANDGWGLKK AADGAAEPGV VLQMLEAAEE RPWLWVVYIL TVALPVFLVI
510 520 530 540 550
LFCCSGKKQS NAMEYKKTDA PQPDVKDEEG KEEEKNKRDE EEEEEKLEEK
560 570 580 590
QKSDAEEDGV TGSQDEEDSK PKAEEDEILN RSPRNRKPRR E
Length:591
Mass (Da):67,278
Last modified:June 1, 1994 - v1
Checksum:i0D9F8FE03434BADC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti416K → R in AAA62450 (PubMed:8148318).Curated1
Sequence conflicti468P → L in AAA62450 (PubMed:8148318).Curated1
Sequence conflicti472L → G in AAA62450 (PubMed:8148318).Curated1
Sequence conflicti538R → G in AAA62450 (PubMed:8148318).Curated1
Sequence conflicti560V → G in AAA62450 (PubMed:8148318).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L18888 mRNA Translation: AAA21014.1
BC012408 mRNA Translation: AAH12408.1
BC040244 mRNA Translation: AAH40244.1
AK084175 mRNA Translation: BAC39133.1
L23865 mRNA Translation: AAA62450.1
CCDSiCCDS24633.1
PIRiB54354
RefSeqiNP_001103969.1, NM_001110499.1
NP_001103970.1, NM_001110500.1
NP_031623.1, NM_007597.3
UniGeneiMm.248827

Genome annotation databases

EnsembliENSMUST00000020637; ENSMUSP00000020637; ENSMUSG00000020368
ENSMUST00000179865; ENSMUSP00000137440; ENSMUSG00000020368
GeneIDi12330
KEGGimmu:12330
UCSCiuc007isf.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCALX_MOUSE
AccessioniPrimary (citable) accession number: P35564
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 25, 2018
This is version 175 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome