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Protein

5-hydroxytryptamine receptor 3A

Gene

Htr3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cation transmembrane transport Source: GOC
  • cellular response to growth factor stimulus Source: RGD
  • potassium ion transmembrane transport Source: GOC
  • response to cocaine Source: RGD
  • response to ethanol Source: RGD
  • synaptic transmission Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-975298. Ligand-gated ion channel transport.

Protein family/group databases

TCDBi1.A.9.2.2. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 3A
Short name:
5-HT3-A
Short name:
5-HT3A
Alternative name(s):
5-hydroxytryptamine receptor 3
Short name:
5-HT-3
Short name:
5-HT3R
Serotonin receptor 3A
Serotonin-gated ion channel receptor
Gene namesi
Name:Htr3a
Synonyms:5ht3, Htr3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi61818. Htr3a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 246223ExtracellularSequence analysisAdd
BLAST
Transmembranei247 – 27327Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini274 – 2785CytoplasmicSequence analysis
Transmembranei279 – 29719Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini298 – 30710ExtracellularSequence analysis
Transmembranei308 – 32619Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini327 – 460134CytoplasmicSequence analysisAdd
BLAST
Transmembranei461 – 48020Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini481 – 4833ExtracellularSequence analysis

GO - Cellular componenti

  • axon Source: RGD
  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: RGD
  • integral component of plasma membrane Source: RGD
  • neuronal cell body Source: RGD
  • plasma membrane Source: RGD
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2094116.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 4834605-hydroxytryptamine receptor 3APRO_0000000410Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence analysis
Disulfide bondi162 ↔ 176By similarity
Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence analysis
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP35563.

PTM databases

PhosphoSiteiP35563.

Expressioni

Tissue specificityi

Expressed in central and peripheral neurons.1 Publication

Inductioni

By nerve growth factor in PC12 cells.1 Publication

Gene expression databases

GenevisibleiP35563. RN.

Interactioni

Subunit structurei

Interacts with RIC3 (By similarity). Forms pentahomomeric complex as well as pentaheteromeric complex with HTR3B; homomeric complex is functional but exhibits low conductance with modified voltage dependence and antagonist affinity.By similarity2 Publications

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008965.

Chemistry

BindingDBiP35563.

Structurei

3D structure databases

ProteinModelPortaliP35563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni419 – 45537HA-stretchAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00790000122957.
HOGENOMiHOG000241519.
HOVERGENiHBG106638.
InParanoidiP35563.
KOiK04819.
OMAiHGEVQNY.
OrthoDBiEOG7KDF9H.
PhylomeDBiP35563.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR008132. 5HT3_rcpt.
IPR008133. 5HT3_rcpt_A.
IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01709. 5HT3ARECEPTR.
PR01708. 5HT3RECEPTOR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLCIPQVLL ALFLSVLIAQ GEGSRRRATQ AHSTTQPALL RLSDHLLANY
60 70 80 90 100
KKGVRPVRDW RKPTLVSIDV IMYAILNVDE KNQVLTTYIW YRQFWTDEFL
110 120 130 140 150
QWTPEDFDNV TKLSIPTDSI WVPDILINEF VDVGKSPSIP YVYVHHQGEV
160 170 180 190 200
QNYKPLQLVT ACSLDIYNFP FDVQNCSLTF TSWLHTIQDI NISLWRTPEE
210 220 230 240 250
VRSDKSIFIN QGEWELLGVF TKFQEFSIET SNSYAEMKFY VVIRRRPLFY
260 270 280 290 300
AVSLLLPSIF LMVVDIVGFC LPPDSGERVS FKITLLLGYS VFLIIVSDTL
310 320 330 340 350
PATAIGTPLI GVYFVVCMAL LVISLAETIF IVQLVHKQDL QRPVPDWLRH
360 370 380 390 400
LVLDRIAWLL CLGEQPMAHR PPATFQANKT DDCSAMGNHC SHVGSPQDLE
410 420 430 440 450
KTSRSRDSPL PPPREASLAV RGLLQELSSI RHSLEKRDEM REVARDWLRV
460 470 480
GYVLDRLLFR IYLLAVLAYS ITLVTLWSIW HYS
Length:483
Mass (Da):55,428
Last modified:November 1, 1995 - v2
Checksum:iED85257BBCCF28A4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221E → K in AAA52182 (PubMed:7509203).Curated
Sequence conflicti306 – 3061G → R in AAA52182 (PubMed:7509203).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49395 mRNA. Translation: BAA08388.1.
U01227 mRNA. Translation: AAA52182.1.
PIRiI58179.
RefSeqiNP_077370.2. NM_024394.2.
UniGeneiRn.55109.

Genome annotation databases

EnsembliENSRNOT00000008965; ENSRNOP00000008965; ENSRNOG00000006595.
GeneIDi79246.
KEGGirno:79246.
UCSCiRGD:61818. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49395 mRNA. Translation: BAA08388.1.
U01227 mRNA. Translation: AAA52182.1.
PIRiI58179.
RefSeqiNP_077370.2. NM_024394.2.
UniGeneiRn.55109.

3D structure databases

ProteinModelPortaliP35563.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008965.

Chemistry

BindingDBiP35563.
ChEMBLiCHEMBL2094116.

Protein family/group databases

TCDBi1.A.9.2.2. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

PTM databases

PhosphoSiteiP35563.

Proteomic databases

PaxDbiP35563.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000008965; ENSRNOP00000008965; ENSRNOG00000006595.
GeneIDi79246.
KEGGirno:79246.
UCSCiRGD:61818. rat.

Organism-specific databases

CTDi3359.
RGDi61818. Htr3a.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00790000122957.
HOGENOMiHOG000241519.
HOVERGENiHBG106638.
InParanoidiP35563.
KOiK04819.
OMAiHGEVQNY.
OrthoDBiEOG7KDF9H.
PhylomeDBiP35563.
TreeFamiTF315605.

Enzyme and pathway databases

ReactomeiR-RNO-975298. Ligand-gated ion channel transport.

Miscellaneous databases

NextBioi614706.
PROiP35563.

Gene expression databases

GenevisibleiP35563. RN.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR008132. 5HT3_rcpt.
IPR008133. 5HT3_rcpt_A.
IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01709. 5HT3ARECEPTR.
PR01708. 5HT3RECEPTOR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Miyake A., Mochizuki S., Akuzawa S., Kon G.
    Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Partial cDNA cloning and NGF regulation of a rat 5-HT3 receptor subunit."
    Isenberg K.E., Ukhun I.A., Holstad S.G., Jafri S., Uchida U., Zorumski C.F., Yang J.
    NeuroReport 5:121-124(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 22-483.
  3. "Evidence for expression of heteromeric serotonin 5-HT(3) receptors in rodents."
    Hanna M.C., Davies P.A., Hales T.G., Kirkness E.F.
    J. Neurochem. 75:240-247(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INDUCTION.
  4. "Differential composition of 5-hydroxytryptamine3 receptors synthesized in the rat CNS and peripheral nervous system."
    Morales M., Wang S.-D.
    J. Neurosci. 22:6732-6741(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, TISSUE SPECIFICITY.
  5. "A cytoplasmic region determines single-channel conductance in 5-HT3 receptors."
    Kelley S.P., Dunlop J.I., Kirkness E.F., Lambert J.J., Peters J.A.
    Nature 424:321-324(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REGION.

Entry informationi

Entry namei5HT3A_RAT
AccessioniPrimary (citable) accession number: P35563
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1995
Last modified: May 11, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The HA-stretch region of HTR3A seems to be responsible for the low conductance of HTR3A homomers compared to that of HTR3A/HTR3B heteromers.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.