Skip Header

Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P35561 (IRK2_MOUSE)

Last modified February 9, 2010. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Inward rectifier potassium channel 2
Alternative name(s):
    Potassium channel, inwardly rectifying subfamily J member 2
    Inward rectifier K(+) channel Kir2.1
      Short name=IRK-1
Gene names
Name: Kcnj2
Synonyms: Irk1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Hide | Top

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.

Subunit structure

Homomultimeric and heteromultimeric association with Kir2.3, resulting in an enhanced G-protein-induced current. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Prominently expressed in the central nervous system. Also found in other excitable tissues such as heart and skeletal muscle.

Sequence similarities

Belongs to the inward rectifier-type potassium channel family.

Hide | Top

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
   LigandPotassium
   Molecular functionIonic channel
Voltage-gated channel
   PTMPhosphoprotein
   Technical term3D-structure
Gene Ontology (GO)
   Biological processpotassium ion transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioninward rectifier potassium channel activity

Inferred from electronic annotation. Source: InterPro

potassium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Hide | Top

Binary interactions

With

Entry

#Exp.

IntAct

Notes

AKAP5P245882EBI-703793,EBI-703640From a different organism.
DLG2Q15700-42EBI-703793,EBI-663057From a different organism.
FLNAQ2YHQ33EBI-703793,EBI-779542From a different organism.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 428428Inward rectifier potassium channel 2
PRO_0000154925

Regions

Topological domain1 – 8181Cytoplasmic By similarity
Transmembrane82 – 10625M1 By similarity
Topological domain107 – 12822Extracellular By similarity
Topological domain148 – 1569Extracellular By similarity
Transmembrane157 – 17822M2 By similarity
Topological domain179 – 428250Cytoplasmic By similarity
Region129 – 14012H5 (pore-forming helix) By similarity
Motif142 – 1476Selectivity filter By similarity
Motif426 – 4283PDZ-binding Potential

Sites

Site1721Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Amino acid modifications

Modified residue3371Phosphotyrosine By similarity

Secondary structure

1 .......................................... 428
Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P35561-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 5B4219F979BBA41C

FASTA42848,389
        10         20         30         40         50         60 
MGSVRTNRYS IVSSEEDGMK LATMAVANGF GNGKSKVHTR QQCRSRFVKK DGHCNVQFIN 

        70         80         90        100        110        120 
VGEKGQRYLA DIFTTCVDIR WRWMLVIFCL AFVLSWLFFG CVFWLIALLH GDLDTSKVSK 

       130        140        150        160        170        180 
ACVSEVNSFT AAFLFSIETQ TTIGYGFRCV TDECPIAVFM VVFQSIVGCI IDAFIIGAVM 

       190        200        210        220        230        240 
AKMAKPKKRN ETLVFSHNAV IAMRDGKLCL MWRVGNLRKS HLVEAHVRAQ LLKSRITSEG 

       250        260        270        280        290        300 
EYIPLDQIDI NVGFDSGIDR IFLVSPITIV HEIDEDSPLY DLSKQDIDNA DFEIVVILEG 

       310        320        330        340        350        360 
MVEATAMTTQ CRSSYLANEI LWGHRYEPVL FEEKHYYKVD YSRFHKTYEV PNTPLCSARD 

       370        380        390        400        410        420 
LAEKKYILSN ANSFCYENEV ALTSKEEEED SENGVPESTS TDSPPGIDLH NQASVPLEPR 


PLRRESEI

« Hide

Hide | Top

References

[1]"Primary structure and functional expression of a mouse inward rectifier potassium channel."
Kubo Y., Baldwin T.J., Jan Y.N., Jan L.Y.
Nature 362:127-132(1993) [PubMed: 7680768] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Macrophage.
[2]"Molecular cloning, functional expression and localization of an inward rectifier potassium channel in the mouse brain."
Morishige K., Takahashi N., Findlay I., Koyama H., Zanelli J.S., Peterson C., Jenkins N.A., Copeland N.G., Mori N., Kurachi Y.
FEBS Lett. 336:375-380(1993) [PubMed: 8282096] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"Inwardly rectifying potassium channels in lens epithelium are from the IRK1 (Kir 2.1) family."
Rae J.L., Shepard A.R.
Exp. Eye Res. 66:347-359(1998) [PubMed: 9533862] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lens epithelium.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X73052 mRNA. Translation: CAA51526.1.
AF021136 mRNA. Translation: AAB88794.1.
IPIIPI00119613.
PIRS32351.
RefSeqNP_032451.1.
UniGeneMm.4951

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1U4FX-ray2.41A/B/C/D41-428[»]
2GIXX-ray2.02A/B/C/D44-371[»]
SMRP35561. Positions 44-348.
ModBaseSearch...

Protein-protein interaction databases

IntActP35561. 6 interactions.
STRINGP35561.

PTM databases

PhosphoSiteP35561.

Proteomic databases

PRIDEP35561.

Genome annotation databases

EnsemblENSMUST00000042970; ENSMUSP00000037192; ENSMUSG00000041695; Mus musculus. [Genome view]
GeneID16518.
KEGGmmu:16518.
UCSCuc007mdw.1. mouse.

Organism-specific databases

CTD16518.
MGIMGI:104744. Kcnj2.

Phylogenomic databases

eggNOGroNOG05227.
HOGENOMHBG716702.
HOVERGENP35561.
InParanoidP35561.
OMAQVSSFTA.
OrthoDBEOG9R26DF.
PhylomeDBP35561.

Gene expression databases

ArrayExpressP35561.
BgeeP35561.
GenevestigatorP35561.
GermOnlineENSMUSG00000041695. Mus musculus.

Family and domain databases

InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR001838. K_chnl_inward-rec_Kir-like.
IPR003271. K_chnl_inward-rec_Kir2.1.
IPR013521. K_chnl_inward-rec_Kir_Cr2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
IPR013673. K_chnl_inward-rec_Kir_N.
[Graphical view]
Gene3DG3DSA:2.60.40.1400. IR_K+channel_cytopl. 1 hit.
PANTHERPTHR11767. K+channel_IR. 1 hit.
PTHR11767:SF15. KIR21_channel. 1 hit.
PfamPF01007. IRK. 1 hit.
PF08466. IRK_N. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01324. KIR21CHANNEL.
PR01320. KIRCHANNEL.
ProtoNetSearch...

Other Resources

NextBio289885.
SOURCESearch...

Hide | Top

Entry information

Entry nameIRK2_MOUSE
AccessionPrimary (citable) accession number: P35561
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 9, 2010
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Hide | Top

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents