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P35561

- KCNJ2_MOUSE

UniProt

P35561 - KCNJ2_MOUSE

Protein

Inward rectifier potassium channel 2

Gene

Kcnj2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei172 – 1721Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. inward rectifier potassium channel activity Source: UniProtKB
    3. phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl
    4. protein binding Source: IntAct
    5. voltage-gated potassium channel activity involved in cardiac muscle cell action potential repolarization Source: Ensembl

    GO - Biological processi

    1. cardiac muscle cell action potential Source: MGI
    2. cardiac muscle cell action potential involved in contraction Source: Ensembl
    3. cellular response to mechanical stimulus Source: Ensembl
    4. magnesium ion transport Source: MGI
    5. positive regulation of potassium ion transmembrane transport Source: Ensembl
    6. potassium ion import Source: Ensembl
    7. potassium ion transport Source: UniProtKB
    8. protein homotetramerization Source: UniProtKB
    9. regulation of heart rate by cardiac conduction Source: Ensembl
    10. regulation of membrane repolarization Source: Ensembl
    11. regulation of skeletal muscle contraction via regulation of action potential Source: Ensembl
    12. relaxation of cardiac muscle Source: Ensembl
    13. relaxation of skeletal muscle Source: Ensembl

    Keywords - Molecular functioni

    Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199076. Classical Kir channels.
    REACT_208837. Activation of G protein gated Potassium channels.
    REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inward rectifier potassium channel 2
    Alternative name(s):
    Inward rectifier K(+) channel Kir2.1
    Short name:
    IRK-1
    Potassium channel, inwardly rectifying subfamily J member 2
    Gene namesi
    Name:Kcnj2
    Synonyms:Irk1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:104744. Kcnj2.

    Subcellular locationi

    GO - Cellular componenti

    1. dendritic spine Source: Ensembl
    2. Golgi apparatus Source: Ensembl
    3. intercalated disc Source: Ensembl
    4. intrinsic component of membrane Source: UniProtKB
    5. neuronal cell body Source: Ensembl
    6. rough endoplasmic reticulum Source: Ensembl
    7. smooth endoplasmic reticulum Source: Ensembl
    8. T-tubule Source: Ensembl
    9. voltage-gated potassium channel complex Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi75 – 751T → R: The mutant protein is able to coassemble and traffic to the cell membrane. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 428428Inward rectifier potassium channel 2PRO_0000154925Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei76 – 761S-nitrosocysteineBy similarity

    Post-translational modificationi

    S-nitrosylation increases the open probabilty and inward rectifying currents.By similarity

    Keywords - PTMi

    S-nitrosylation

    Proteomic databases

    PaxDbiP35561.
    PRIDEiP35561.

    PTM databases

    PhosphoSiteiP35561.

    Expressioni

    Tissue specificityi

    Prominently expressed in the central nervous system. Also found in other excitable tissues such as heart and skeletal muscle.

    Gene expression databases

    BgeeiP35561.
    GenevestigatoriP35561.

    Interactioni

    Subunit structurei

    Homomultimeric and heteromultimeric association with KCNJ4/Kir2.3. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself7EBI-703793,EBI-703793
    AKAP5P245882EBI-703793,EBI-703640From a different organism.
    Ap1g1B2RYN62EBI-703793,EBI-4319005From a different organism.
    DLG2Q15700-46EBI-703793,EBI-663057From a different organism.
    FLNAQ2YHQ33EBI-703793,EBI-779542From a different organism.

    Protein-protein interaction databases

    BioGridi200900. 1 interaction.
    IntActiP35561. 11 interactions.
    MINTiMINT-104054.
    STRINGi10090.ENSMUSP00000037192.

    Structurei

    Secondary structure

    1
    428
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi56 – 594
    Turni188 – 1903
    Beta strandi192 – 1954
    Beta strandi199 – 2046
    Beta strandi207 – 21610
    Beta strandi218 – 2203
    Beta strandi222 – 23615
    Beta strandi242 – 2509
    Turni254 – 2596
    Beta strandi267 – 2726
    Turni278 – 2814
    Helixi284 – 2885
    Beta strandi293 – 30210
    Turni303 – 3053
    Beta strandi308 – 3169
    Helixi317 – 3193
    Beta strandi320 – 3223
    Beta strandi324 – 3263
    Beta strandi330 – 3323
    Beta strandi334 – 3407
    Helixi341 – 3433
    Beta strandi347 – 3493
    Helixi358 – 36912

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1U4FX-ray2.41A/B/C/D41-428[»]
    2GIXX-ray2.02A/B/C/D44-371[»]
    2XKYOther17.20I/J/K/L1-67[»]
    I/J/K/L189-428[»]
    ProteinModelPortaliP35561.
    SMRiP35561. Positions 43-371.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP35561.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8181CytoplasmicBy similarityAdd
    BLAST
    Topological domaini107 – 12822ExtracellularBy similarityAdd
    BLAST
    Topological domaini148 – 1569ExtracellularBy similarity
    Topological domaini179 – 428250CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei129 – 14012Helical; Pore-forming; Name=H5By similarityAdd
    BLAST
    Intramembranei141 – 1477Pore-formingBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei82 – 10625Helical; Name=M1By similarityAdd
    BLAST
    Transmembranei157 – 17822Helical; Name=M2By similarityAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi142 – 1476Selectivity filterBy similarity
    Motifi426 – 4283PDZ-bindingSequence Analysis

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG72812.
    HOGENOMiHOG000237325.
    HOVERGENiHBG006178.
    InParanoidiP35561.
    KOiK04996.
    OMAiHNATVAM.
    OrthoDBiEOG7XPZ5K.
    PhylomeDBiP35561.
    TreeFamiTF313676.

    Family and domain databases

    Gene3Di2.60.40.1400. 1 hit.
    InterProiIPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003271. K_chnl_inward-rec_Kir2.1.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    IPR013673. K_chnl_inward-rec_Kir_N.
    [Graphical view]
    PANTHERiPTHR11767. PTHR11767. 1 hit.
    PfamiPF01007. IRK. 1 hit.
    PF08466. IRK_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005465. GIRK_kir. 1 hit.
    PRINTSiPR01324. KIR21CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P35561-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSVRTNRYS IVSSEEDGMK LATMAVANGF GNGKSKVHTR QQCRSRFVKK    50
    DGHCNVQFIN VGEKGQRYLA DIFTTCVDIR WRWMLVIFCL AFVLSWLFFG 100
    CVFWLIALLH GDLDTSKVSK ACVSEVNSFT AAFLFSIETQ TTIGYGFRCV 150
    TDECPIAVFM VVFQSIVGCI IDAFIIGAVM AKMAKPKKRN ETLVFSHNAV 200
    IAMRDGKLCL MWRVGNLRKS HLVEAHVRAQ LLKSRITSEG EYIPLDQIDI 250
    NVGFDSGIDR IFLVSPITIV HEIDEDSPLY DLSKQDIDNA DFEIVVILEG 300
    MVEATAMTTQ CRSSYLANEI LWGHRYEPVL FEEKHYYKVD YSRFHKTYEV 350
    PNTPLCSARD LAEKKYILSN ANSFCYENEV ALTSKEEEED SENGVPESTS 400
    TDSPPGIDLH NQASVPLEPR PLRRESEI 428
    Length:428
    Mass (Da):48,389
    Last modified:June 1, 1994 - v1
    Checksum:i5B4219F979BBA41C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73052 mRNA. Translation: CAA51526.1.
    AF021136 mRNA. Translation: AAB88794.1.
    CCDSiCCDS25594.1.
    PIRiS32351.
    RefSeqiNP_032451.1. NM_008425.4.
    UniGeneiMm.4951.

    Genome annotation databases

    EnsembliENSMUST00000042970; ENSMUSP00000037192; ENSMUSG00000041695.
    GeneIDi16518.
    KEGGimmu:16518.
    UCSCiuc007mdw.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73052 mRNA. Translation: CAA51526.1 .
    AF021136 mRNA. Translation: AAB88794.1 .
    CCDSi CCDS25594.1.
    PIRi S32351.
    RefSeqi NP_032451.1. NM_008425.4.
    UniGenei Mm.4951.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1U4F X-ray 2.41 A/B/C/D 41-428 [» ]
    2GIX X-ray 2.02 A/B/C/D 44-371 [» ]
    2XKY Other 17.20 I/J/K/L 1-67 [» ]
    I/J/K/L 189-428 [» ]
    ProteinModelPortali P35561.
    SMRi P35561. Positions 43-371.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200900. 1 interaction.
    IntActi P35561. 11 interactions.
    MINTi MINT-104054.
    STRINGi 10090.ENSMUSP00000037192.

    Chemistry

    BindingDBi P35561.
    ChEMBLi CHEMBL1293290.
    GuidetoPHARMACOLOGYi 430.

    PTM databases

    PhosphoSitei P35561.

    Proteomic databases

    PaxDbi P35561.
    PRIDEi P35561.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000042970 ; ENSMUSP00000037192 ; ENSMUSG00000041695 .
    GeneIDi 16518.
    KEGGi mmu:16518.
    UCSCi uc007mdw.2. mouse.

    Organism-specific databases

    CTDi 3759.
    MGIi MGI:104744. Kcnj2.

    Phylogenomic databases

    eggNOGi NOG72812.
    HOGENOMi HOG000237325.
    HOVERGENi HBG006178.
    InParanoidi P35561.
    KOi K04996.
    OMAi HNATVAM.
    OrthoDBi EOG7XPZ5K.
    PhylomeDBi P35561.
    TreeFami TF313676.

    Enzyme and pathway databases

    Reactomei REACT_199076. Classical Kir channels.
    REACT_208837. Activation of G protein gated Potassium channels.
    REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

    Miscellaneous databases

    EvolutionaryTracei P35561.
    NextBioi 289885.
    PROi P35561.
    SOURCEi Search...

    Gene expression databases

    Bgeei P35561.
    Genevestigatori P35561.

    Family and domain databases

    Gene3Di 2.60.40.1400. 1 hit.
    InterProi IPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003271. K_chnl_inward-rec_Kir2.1.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    IPR013673. K_chnl_inward-rec_Kir_N.
    [Graphical view ]
    PANTHERi PTHR11767. PTHR11767. 1 hit.
    Pfami PF01007. IRK. 1 hit.
    PF08466. IRK_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005465. GIRK_kir. 1 hit.
    PRINTSi PR01324. KIR21CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure and functional expression of a mouse inward rectifier potassium channel."
      Kubo Y., Baldwin T.J., Jan Y.N., Jan L.Y.
      Nature 362:127-132(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Macrophage.
    2. "Molecular cloning, functional expression and localization of an inward rectifier potassium channel in the mouse brain."
      Morishige K., Takahashi N., Findlay I., Koyama H., Zanelli J.S., Peterson C., Jenkins N.A., Copeland N.G., Mori N., Kurachi Y.
      FEBS Lett. 336:375-380(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    3. "Inwardly rectifying potassium channels in lens epithelium are from the IRK1 (Kir 2.1) family."
      Rae J.L., Shepard A.R.
      Exp. Eye Res. 66:347-359(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lens epithelium.
    4. "Functional and clinical characterization of a mutation in KCNJ2 associated with Andersen-Tawil syndrome."
      Lu C.W., Lin J.H., Rajawat Y.S., Jerng H., Rami T.G., Sanchez X., DeFreitas G., Carabello B., DeMayo F., Kearney D.L., Miller G., Li H., Pfaffinger P.J., Bowles N.E., Khoury D.S., Towbin J.A.
      J. Med. Genet. 43:653-659(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF THR-75.
    5. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKCNJ2_MOUSE
    AccessioniPrimary (citable) accession number: P35561
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3