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P35561

- KCNJ2_MOUSE

UniProt

P35561 - KCNJ2_MOUSE

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Protein

Inward rectifier potassium channel 2

Gene

Kcnj2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei172 – 1721Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. inward rectifier potassium channel activity Source: UniProtKB
  3. phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl
  4. voltage-gated potassium channel activity involved in cardiac muscle cell action potential repolarization Source: Ensembl

GO - Biological processi

  1. cardiac muscle cell action potential Source: MGI
  2. cardiac muscle cell action potential involved in contraction Source: Ensembl
  3. cellular response to mechanical stimulus Source: Ensembl
  4. magnesium ion transport Source: MGI
  5. positive regulation of potassium ion transmembrane transport Source: Ensembl
  6. potassium ion import Source: Ensembl
  7. potassium ion transport Source: UniProtKB
  8. protein homotetramerization Source: UniProtKB
  9. regulation of heart rate by cardiac conduction Source: Ensembl
  10. regulation of membrane repolarization Source: Ensembl
  11. regulation of skeletal muscle contraction via regulation of action potential Source: Ensembl
  12. relaxation of cardiac muscle Source: Ensembl
  13. relaxation of skeletal muscle Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_199076. Classical Kir channels.
REACT_208837. Activation of G protein gated Potassium channels.
REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
Inward rectifier potassium channel 2
Alternative name(s):
Inward rectifier K(+) channel Kir2.1
Short name:
IRK-1
Potassium channel, inwardly rectifying subfamily J member 2
Gene namesi
Name:Kcnj2
Synonyms:Irk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:104744. Kcnj2.

Subcellular locationi

Membrane; Multi-pass membrane protein. Membrane; Lipid-anchor By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8181CytoplasmicBy similarityAdd
BLAST
Transmembranei82 – 10625Helical; Name=M1By similarityAdd
BLAST
Topological domaini107 – 12822ExtracellularBy similarityAdd
BLAST
Intramembranei129 – 14012Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei141 – 1477Pore-formingBy similarity
Topological domaini148 – 1569ExtracellularBy similarity
Transmembranei157 – 17822Helical; Name=M2By similarityAdd
BLAST
Topological domaini179 – 428250CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. dendritic spine Source: Ensembl
  2. Golgi apparatus Source: Ensembl
  3. intercalated disc Source: Ensembl
  4. intrinsic component of membrane Source: UniProtKB
  5. neuronal cell body Source: Ensembl
  6. rough endoplasmic reticulum Source: Ensembl
  7. smooth endoplasmic reticulum Source: Ensembl
  8. T-tubule Source: Ensembl
  9. voltage-gated potassium channel complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi75 – 751T → R: The mutant protein is able to coassemble and traffic to the cell membrane. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Inward rectifier potassium channel 2PRO_0000154925Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei76 – 761S-nitrosocysteineBy similarity

Post-translational modificationi

S-nitrosylation increases the open probabilty and inward rectifying currents.By similarity

Keywords - PTMi

Lipoprotein, Myristate, S-nitrosylation

Proteomic databases

PaxDbiP35561.
PRIDEiP35561.

PTM databases

PhosphoSiteiP35561.

Expressioni

Tissue specificityi

Prominently expressed in the central nervous system. Also found in other excitable tissues such as heart and skeletal muscle.

Gene expression databases

BgeeiP35561.
ExpressionAtlasiP35561. baseline and differential.
GenevestigatoriP35561.

Interactioni

Subunit structurei

Homomultimeric and heteromultimeric association with KCNJ4/Kir2.3. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself7EBI-703793,EBI-703793
AKAP5P245882EBI-703793,EBI-703640From a different organism.
Ap1g1B2RYN62EBI-703793,EBI-4319005From a different organism.
DLG2Q15700-46EBI-703793,EBI-663057From a different organism.
FLNAQ2YHQ33EBI-703793,EBI-779542From a different organism.

Protein-protein interaction databases

BioGridi200900. 1 interaction.
IntActiP35561. 11 interactions.
MINTiMINT-104054.
STRINGi10090.ENSMUSP00000037192.

Structurei

Secondary structure

1
428
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi56 – 594Combined sources
Turni188 – 1903Combined sources
Beta strandi192 – 1954Combined sources
Beta strandi199 – 2046Combined sources
Beta strandi207 – 21610Combined sources
Beta strandi218 – 2203Combined sources
Beta strandi222 – 23615Combined sources
Beta strandi242 – 2509Combined sources
Turni254 – 2596Combined sources
Beta strandi267 – 2726Combined sources
Turni278 – 2814Combined sources
Helixi284 – 2885Combined sources
Beta strandi293 – 30210Combined sources
Turni303 – 3053Combined sources
Beta strandi308 – 3169Combined sources
Helixi317 – 3193Combined sources
Beta strandi320 – 3223Combined sources
Beta strandi324 – 3263Combined sources
Beta strandi330 – 3323Combined sources
Beta strandi334 – 3407Combined sources
Helixi341 – 3433Combined sources
Beta strandi347 – 3493Combined sources
Helixi358 – 36912Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U4FX-ray2.41A/B/C/D41-428[»]
2GIXX-ray2.02A/B/C/D44-371[»]
2XKYOther17.20I/J/K/L1-67[»]
I/J/K/L189-428[»]
ProteinModelPortaliP35561.
SMRiP35561. Positions 43-371.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35561.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi142 – 1476Selectivity filterBy similarity
Motifi426 – 4283PDZ-bindingSequence Analysis

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG72812.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP35561.
KOiK04996.
OMAiHNATVAM.
OrthoDBiEOG7XPZ5K.
PhylomeDBiP35561.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003271. K_chnl_inward-rec_Kir2.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
IPR013673. K_chnl_inward-rec_Kir_N.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
PF08466. IRK_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01324. KIR21CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P35561-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSVRTNRYS IVSSEEDGMK LATMAVANGF GNGKSKVHTR QQCRSRFVKK
60 70 80 90 100
DGHCNVQFIN VGEKGQRYLA DIFTTCVDIR WRWMLVIFCL AFVLSWLFFG
110 120 130 140 150
CVFWLIALLH GDLDTSKVSK ACVSEVNSFT AAFLFSIETQ TTIGYGFRCV
160 170 180 190 200
TDECPIAVFM VVFQSIVGCI IDAFIIGAVM AKMAKPKKRN ETLVFSHNAV
210 220 230 240 250
IAMRDGKLCL MWRVGNLRKS HLVEAHVRAQ LLKSRITSEG EYIPLDQIDI
260 270 280 290 300
NVGFDSGIDR IFLVSPITIV HEIDEDSPLY DLSKQDIDNA DFEIVVILEG
310 320 330 340 350
MVEATAMTTQ CRSSYLANEI LWGHRYEPVL FEEKHYYKVD YSRFHKTYEV
360 370 380 390 400
PNTPLCSARD LAEKKYILSN ANSFCYENEV ALTSKEEEED SENGVPESTS
410 420
TDSPPGIDLH NQASVPLEPR PLRRESEI
Length:428
Mass (Da):48,389
Last modified:June 1, 1994 - v1
Checksum:i5B4219F979BBA41C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73052 mRNA. Translation: CAA51526.1.
AF021136 mRNA. Translation: AAB88794.1.
CCDSiCCDS25594.1.
PIRiS32351.
RefSeqiNP_032451.1. NM_008425.4.
UniGeneiMm.4951.

Genome annotation databases

EnsembliENSMUST00000042970; ENSMUSP00000037192; ENSMUSG00000041695.
GeneIDi16518.
KEGGimmu:16518.
UCSCiuc007mdw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73052 mRNA. Translation: CAA51526.1 .
AF021136 mRNA. Translation: AAB88794.1 .
CCDSi CCDS25594.1.
PIRi S32351.
RefSeqi NP_032451.1. NM_008425.4.
UniGenei Mm.4951.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1U4F X-ray 2.41 A/B/C/D 41-428 [» ]
2GIX X-ray 2.02 A/B/C/D 44-371 [» ]
2XKY Other 17.20 I/J/K/L 1-67 [» ]
I/J/K/L 189-428 [» ]
ProteinModelPortali P35561.
SMRi P35561. Positions 43-371.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200900. 1 interaction.
IntActi P35561. 11 interactions.
MINTi MINT-104054.
STRINGi 10090.ENSMUSP00000037192.

Chemistry

BindingDBi P35561.
ChEMBLi CHEMBL1293290.
GuidetoPHARMACOLOGYi 430.

PTM databases

PhosphoSitei P35561.

Proteomic databases

PaxDbi P35561.
PRIDEi P35561.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000042970 ; ENSMUSP00000037192 ; ENSMUSG00000041695 .
GeneIDi 16518.
KEGGi mmu:16518.
UCSCi uc007mdw.2. mouse.

Organism-specific databases

CTDi 3759.
MGIi MGI:104744. Kcnj2.

Phylogenomic databases

eggNOGi NOG72812.
HOGENOMi HOG000237325.
HOVERGENi HBG006178.
InParanoidi P35561.
KOi K04996.
OMAi HNATVAM.
OrthoDBi EOG7XPZ5K.
PhylomeDBi P35561.
TreeFami TF313676.

Enzyme and pathway databases

Reactomei REACT_199076. Classical Kir channels.
REACT_208837. Activation of G protein gated Potassium channels.
REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

EvolutionaryTracei P35561.
NextBioi 289885.
PROi P35561.
SOURCEi Search...

Gene expression databases

Bgeei P35561.
ExpressionAtlasi P35561. baseline and differential.
Genevestigatori P35561.

Family and domain databases

Gene3Di 2.60.40.1400. 1 hit.
InterProi IPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003271. K_chnl_inward-rec_Kir2.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
IPR013673. K_chnl_inward-rec_Kir_N.
[Graphical view ]
PANTHERi PTHR11767. PTHR11767. 1 hit.
Pfami PF01007. IRK. 1 hit.
PF08466. IRK_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF005465. GIRK_kir. 1 hit.
PRINTSi PR01324. KIR21CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMi SSF81296. SSF81296. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure and functional expression of a mouse inward rectifier potassium channel."
    Kubo Y., Baldwin T.J., Jan Y.N., Jan L.Y.
    Nature 362:127-132(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Macrophage.
  2. "Molecular cloning, functional expression and localization of an inward rectifier potassium channel in the mouse brain."
    Morishige K., Takahashi N., Findlay I., Koyama H., Zanelli J.S., Peterson C., Jenkins N.A., Copeland N.G., Mori N., Kurachi Y.
    FEBS Lett. 336:375-380(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "Inwardly rectifying potassium channels in lens epithelium are from the IRK1 (Kir 2.1) family."
    Rae J.L., Shepard A.R.
    Exp. Eye Res. 66:347-359(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lens epithelium.
  4. "Functional and clinical characterization of a mutation in KCNJ2 associated with Andersen-Tawil syndrome."
    Lu C.W., Lin J.H., Rajawat Y.S., Jerng H., Rami T.G., Sanchez X., DeFreitas G., Carabello B., DeMayo F., Kearney D.L., Miller G., Li H., Pfaffinger P.J., Bowles N.E., Khoury D.S., Towbin J.A.
    J. Med. Genet. 43:653-659(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF THR-75.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKCNJ2_MOUSE
AccessioniPrimary (citable) accession number: P35561
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 26, 2014
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3