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Protein

Inward rectifier potassium channel 2

Gene

Kcnj2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei172Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-MMU-1296041. Activation of G protein gated Potassium channels.
R-MMU-1296053. Classical Kir channels.
R-MMU-5576886. Phase 4 - resting membrane potential.
R-MMU-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
Inward rectifier potassium channel 2
Alternative name(s):
Inward rectifier K(+) channel Kir2.1
Short name:
IRK-1
Potassium channel, inwardly rectifying subfamily J member 2
Gene namesi
Name:Kcnj2
Synonyms:Irk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:104744. Kcnj2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 81CytoplasmicBy similarityAdd BLAST80
Transmembranei82 – 106Helical; Name=M1By similarityAdd BLAST25
Topological domaini107 – 128ExtracellularBy similarityAdd BLAST22
Intramembranei129 – 140Helical; Pore-forming; Name=H5By similarityAdd BLAST12
Intramembranei141 – 147Pore-formingBy similarity7
Topological domaini148 – 156ExtracellularBy similarity9
Transmembranei157 – 178Helical; Name=M2By similarityAdd BLAST22
Topological domaini179 – 428CytoplasmicBy similarityAdd BLAST250

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi75T → R: The mutant protein is able to coassemble and traffic to the cell membrane. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1293290.
GuidetoPHARMACOLOGYi430.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001549252 – 428Inward rectifier potassium channel 2Add BLAST427

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei76S-nitrosocysteineBy similarity1

Post-translational modificationi

S-nitrosylation increases the open probability and inward rectifying currents.By similarity

Keywords - PTMi

Lipoprotein, Myristate, S-nitrosylation

Proteomic databases

PaxDbiP35561.
PeptideAtlasiP35561.
PRIDEiP35561.

PTM databases

iPTMnetiP35561.
PhosphoSitePlusiP35561.

Expressioni

Tissue specificityi

Prominently expressed in the central nervous system. Also found in other excitable tissues such as heart and skeletal muscle.

Gene expression databases

BgeeiENSMUSG00000041695.
ExpressionAtlasiP35561. baseline and differential.
GenevisibleiP35561. MM.

Interactioni

Subunit structurei

Homomultimeric and heteromultimeric association with KCNJ4/Kir2.3. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself7EBI-703793,EBI-703793
AKAP5P245882EBI-703793,EBI-703640From a different organism.
Ap1g1B2RYN62EBI-703793,EBI-4319005From a different organism.
DLG2Q15700-46EBI-703793,EBI-663057From a different organism.
FLNAQ2YHQ33EBI-703793,EBI-779542From a different organism.

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi200900. 2 interactors.
IntActiP35561. 11 interactors.
MINTiMINT-104054.
STRINGi10090.ENSMUSP00000037192.

Chemistry databases

BindingDBiP35561.

Structurei

Secondary structure

1428
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi56 – 59Combined sources4
Turni188 – 190Combined sources3
Beta strandi192 – 195Combined sources4
Beta strandi199 – 204Combined sources6
Beta strandi207 – 216Combined sources10
Beta strandi218 – 220Combined sources3
Beta strandi222 – 236Combined sources15
Beta strandi242 – 250Combined sources9
Turni254 – 259Combined sources6
Beta strandi267 – 272Combined sources6
Turni278 – 281Combined sources4
Helixi284 – 288Combined sources5
Beta strandi293 – 302Combined sources10
Turni303 – 305Combined sources3
Beta strandi308 – 316Combined sources9
Helixi317 – 319Combined sources3
Beta strandi320 – 322Combined sources3
Beta strandi324 – 326Combined sources3
Beta strandi330 – 332Combined sources3
Beta strandi334 – 340Combined sources7
Helixi341 – 343Combined sources3
Beta strandi347 – 349Combined sources3
Helixi358 – 369Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U4FX-ray2.41A/B/C/D41-428[»]
2GIXX-ray2.02A/B/C/D44-371[»]
2XKYOther17.20I/J/K/L1-67[»]
I/J/K/L189-428[»]
ProteinModelPortaliP35561.
SMRiP35561.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35561.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi142 – 147Selectivity filterBy similarity6
Motifi426 – 428PDZ-bindingSequence analysis3

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP35561.
KOiK04996.
OMAiHNATVAM.
OrthoDBiEOG091G08HC.
PhylomeDBiP35561.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003271. K_chnl_inward-rec_Kir2.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
IPR013673. K_chnl_inward-rec_Kir_N.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
PF08466. IRK_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01324. KIR21CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35561-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSVRTNRYS IVSSEEDGMK LATMAVANGF GNGKSKVHTR QQCRSRFVKK
60 70 80 90 100
DGHCNVQFIN VGEKGQRYLA DIFTTCVDIR WRWMLVIFCL AFVLSWLFFG
110 120 130 140 150
CVFWLIALLH GDLDTSKVSK ACVSEVNSFT AAFLFSIETQ TTIGYGFRCV
160 170 180 190 200
TDECPIAVFM VVFQSIVGCI IDAFIIGAVM AKMAKPKKRN ETLVFSHNAV
210 220 230 240 250
IAMRDGKLCL MWRVGNLRKS HLVEAHVRAQ LLKSRITSEG EYIPLDQIDI
260 270 280 290 300
NVGFDSGIDR IFLVSPITIV HEIDEDSPLY DLSKQDIDNA DFEIVVILEG
310 320 330 340 350
MVEATAMTTQ CRSSYLANEI LWGHRYEPVL FEEKHYYKVD YSRFHKTYEV
360 370 380 390 400
PNTPLCSARD LAEKKYILSN ANSFCYENEV ALTSKEEEED SENGVPESTS
410 420
TDSPPGIDLH NQASVPLEPR PLRRESEI
Length:428
Mass (Da):48,389
Last modified:June 1, 1994 - v1
Checksum:i5B4219F979BBA41C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73052 mRNA. Translation: CAA51526.1.
AF021136 mRNA. Translation: AAB88794.1.
CCDSiCCDS25594.1.
PIRiS32351.
RefSeqiNP_032451.1. NM_008425.4.
UniGeneiMm.4951.

Genome annotation databases

EnsembliENSMUST00000042970; ENSMUSP00000037192; ENSMUSG00000041695.
GeneIDi16518.
KEGGimmu:16518.
UCSCiuc007mdw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73052 mRNA. Translation: CAA51526.1.
AF021136 mRNA. Translation: AAB88794.1.
CCDSiCCDS25594.1.
PIRiS32351.
RefSeqiNP_032451.1. NM_008425.4.
UniGeneiMm.4951.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U4FX-ray2.41A/B/C/D41-428[»]
2GIXX-ray2.02A/B/C/D44-371[»]
2XKYOther17.20I/J/K/L1-67[»]
I/J/K/L189-428[»]
ProteinModelPortaliP35561.
SMRiP35561.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200900. 2 interactors.
IntActiP35561. 11 interactors.
MINTiMINT-104054.
STRINGi10090.ENSMUSP00000037192.

Chemistry databases

BindingDBiP35561.
ChEMBLiCHEMBL1293290.
GuidetoPHARMACOLOGYi430.

PTM databases

iPTMnetiP35561.
PhosphoSitePlusiP35561.

Proteomic databases

PaxDbiP35561.
PeptideAtlasiP35561.
PRIDEiP35561.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042970; ENSMUSP00000037192; ENSMUSG00000041695.
GeneIDi16518.
KEGGimmu:16518.
UCSCiuc007mdw.2. mouse.

Organism-specific databases

CTDi3759.
MGIiMGI:104744. Kcnj2.

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP35561.
KOiK04996.
OMAiHNATVAM.
OrthoDBiEOG091G08HC.
PhylomeDBiP35561.
TreeFamiTF313676.

Enzyme and pathway databases

ReactomeiR-MMU-1296041. Activation of G protein gated Potassium channels.
R-MMU-1296053. Classical Kir channels.
R-MMU-5576886. Phase 4 - resting membrane potential.
R-MMU-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

EvolutionaryTraceiP35561.
PROiP35561.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041695.
ExpressionAtlasiP35561. baseline and differential.
GenevisibleiP35561. MM.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003271. K_chnl_inward-rec_Kir2.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
IPR013673. K_chnl_inward-rec_Kir_N.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
PF08466. IRK_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01324. KIR21CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCNJ2_MOUSE
AccessioniPrimary (citable) accession number: P35561
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 161 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.