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Protein

Inward rectifier potassium channel 2

Gene

Kcnj2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei172Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Enzyme and pathway databases

ReactomeiR-MMU-1296041 Activation of G protein gated Potassium channels
R-MMU-1296053 Classical Kir channels
R-MMU-5576886 Phase 4 - resting membrane potential
R-MMU-997272 Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits

Names & Taxonomyi

Protein namesi
Recommended name:
Inward rectifier potassium channel 2
Alternative name(s):
Inward rectifier K(+) channel Kir2.1
Short name:
IRK-1
Potassium channel, inwardly rectifying subfamily J member 2
Gene namesi
Name:Kcnj2
Synonyms:Irk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:104744 Kcnj2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 81CytoplasmicBy similarityAdd BLAST80
Transmembranei82 – 106Helical; Name=M1By similarityAdd BLAST25
Topological domaini107 – 128ExtracellularBy similarityAdd BLAST22
Intramembranei129 – 140Helical; Pore-forming; Name=H5By similarityAdd BLAST12
Intramembranei141 – 147Pore-formingBy similarity7
Topological domaini148 – 156ExtracellularBy similarity9
Transmembranei157 – 178Helical; Name=M2By similarityAdd BLAST22
Topological domaini179 – 428CytoplasmicBy similarityAdd BLAST250

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi75T → R: The mutant protein is able to coassemble and traffic to the cell membrane. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1293290
GuidetoPHARMACOLOGYi430

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001549252 – 428Inward rectifier potassium channel 2Add BLAST427

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei76S-nitrosocysteineBy similarity1

Post-translational modificationi

S-nitrosylation increases the open probability and inward rectifying currents.By similarity

Keywords - PTMi

Lipoprotein, Myristate, S-nitrosylation

Proteomic databases

PaxDbiP35561
PeptideAtlasiP35561
PRIDEiP35561

PTM databases

iPTMnetiP35561
PhosphoSitePlusiP35561
SwissPalmiP35561

Expressioni

Tissue specificityi

Prominently expressed in the central nervous system. Also found in other excitable tissues such as heart and skeletal muscle.

Gene expression databases

BgeeiENSMUSG00000041695
ExpressionAtlasiP35561 baseline and differential
GenevisibleiP35561 MM

Interactioni

Subunit structurei

Homomultimeric and heteromultimeric association with KCNJ4/Kir2.3. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi200900, 2 interactors
IntActiP35561, 11 interactors
MINTiP35561
STRINGi10090.ENSMUSP00000037192

Chemistry databases

BindingDBiP35561

Structurei

Secondary structure

1428
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni188 – 190Combined sources3
Beta strandi192 – 195Combined sources4
Beta strandi199 – 204Combined sources6
Beta strandi207 – 216Combined sources10
Beta strandi218 – 220Combined sources3
Beta strandi222 – 236Combined sources15
Beta strandi242 – 250Combined sources9
Turni254 – 259Combined sources6
Beta strandi267 – 272Combined sources6
Turni278 – 281Combined sources4
Helixi284 – 288Combined sources5
Beta strandi293 – 302Combined sources10
Turni303 – 305Combined sources3
Beta strandi308 – 316Combined sources9
Helixi317 – 319Combined sources3
Beta strandi320 – 322Combined sources3
Beta strandi324 – 326Combined sources3
Beta strandi330 – 332Combined sources3
Beta strandi334 – 340Combined sources7
Helixi341 – 343Combined sources3
Beta strandi347 – 349Combined sources3
Helixi358 – 369Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U4FX-ray2.41A/B/C/D41-63[»]
A/B/C/D188-428[»]
2GIXX-ray2.02A/B/C/D44-64[»]
A/B/C/D189-371[»]
2XKYOther17.20I/J/K/L1-67[»]
I/J/K/L189-428[»]
ProteinModelPortaliP35561
SMRiP35561
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35561

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi142 – 147Selectivity filterBy similarity6
Motifi426 – 428PDZ-bindingSequence analysis3

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827 Eukaryota
ENOG410XQ62 LUCA
GeneTreeiENSGT00760000118842
HOGENOMiHOG000237325
HOVERGENiHBG006178
InParanoidiP35561
KOiK04996
OMAiVFSHNAT
OrthoDBiEOG091G08HC
PhylomeDBiP35561
TreeFamiTF313676

Family and domain databases

Gene3Di2.60.40.1400, 2 hits
InterProiView protein in InterPro
IPR014756 Ig_E-set
IPR016449 K_chnl_inward-rec_Kir
IPR003271 K_chnl_inward-rec_Kir2.1
IPR013518 K_chnl_inward-rec_Kir_cyto
IPR013673 K_chnl_inward-rec_Kir_N
PANTHERiPTHR11767 PTHR11767, 1 hit
PTHR11767:SF43 PTHR11767:SF43, 1 hit
PfamiView protein in Pfam
PF01007 IRK, 1 hit
PF08466 IRK_N, 1 hit
PIRSFiPIRSF005465 GIRK_kir, 1 hit
PRINTSiPR01324 KIR21CHANNEL
PR01320 KIRCHANNEL
SUPFAMiSSF81296 SSF81296, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35561-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSVRTNRYS IVSSEEDGMK LATMAVANGF GNGKSKVHTR QQCRSRFVKK
60 70 80 90 100
DGHCNVQFIN VGEKGQRYLA DIFTTCVDIR WRWMLVIFCL AFVLSWLFFG
110 120 130 140 150
CVFWLIALLH GDLDTSKVSK ACVSEVNSFT AAFLFSIETQ TTIGYGFRCV
160 170 180 190 200
TDECPIAVFM VVFQSIVGCI IDAFIIGAVM AKMAKPKKRN ETLVFSHNAV
210 220 230 240 250
IAMRDGKLCL MWRVGNLRKS HLVEAHVRAQ LLKSRITSEG EYIPLDQIDI
260 270 280 290 300
NVGFDSGIDR IFLVSPITIV HEIDEDSPLY DLSKQDIDNA DFEIVVILEG
310 320 330 340 350
MVEATAMTTQ CRSSYLANEI LWGHRYEPVL FEEKHYYKVD YSRFHKTYEV
360 370 380 390 400
PNTPLCSARD LAEKKYILSN ANSFCYENEV ALTSKEEEED SENGVPESTS
410 420
TDSPPGIDLH NQASVPLEPR PLRRESEI
Length:428
Mass (Da):48,389
Last modified:June 1, 1994 - v1
Checksum:i5B4219F979BBA41C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73052 mRNA Translation: CAA51526.1
AF021136 mRNA Translation: AAB88794.1
CCDSiCCDS25594.1
PIRiS32351
RefSeqiNP_032451.1, NM_008425.4
UniGeneiMm.4951

Genome annotation databases

EnsembliENSMUST00000042970; ENSMUSP00000037192; ENSMUSG00000041695
GeneIDi16518
KEGGimmu:16518
UCSCiuc007mdw.2 mouse

Entry informationi

Entry nameiKCNJ2_MOUSE
AccessioniPrimary (citable) accession number: P35561
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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