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P35560

- KCNJ1_RAT

UniProt

P35560 - KCNJ1_RAT

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Protein

ATP-sensitive inward rectifier potassium channel 1

Gene
Kcnj1, Romk1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium.

Enzyme regulationi

Inhibited by WNK3 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei171 – 1711Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi223 – 2308ATP Reviewed prediction

GO - Molecular functioni

  1. ATP-activated inward rectifier potassium channel activity Source: RGD
  2. ATP binding Source: UniProtKB-KW
  3. inward rectifier potassium channel activity Source: RGD
  4. peptide binding Source: RGD
  5. potassium ion binding Source: RGD

GO - Biological processi

  1. cellular response to magnesium ion Source: RGD
  2. negative regulation of apoptotic process Source: RGD
  3. potassium ion transmembrane transport Source: GOC
  4. potassium ion transport Source: RGD
  5. response to potassium ion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-sensitive inward rectifier potassium channel 1
Alternative name(s):
ATP-regulated potassium channel ROM-K
Inward rectifier K(+) channel Kir1.1
KAB-1
Potassium channel, inwardly rectifying subfamily J member 1
Gene namesi
Name:Kcnj1
Synonyms:Romk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2957. Kcnj1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein
Note: Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7777Cytoplasmic By similarityAdd
BLAST
Transmembranei78 – 10225Helical; Name=M1; By similarityAdd
BLAST
Topological domaini103 – 12725Extracellular By similarityAdd
BLAST
Intramembranei128 – 13912Helical; Pore-forming; Name=H5; By similarityAdd
BLAST
Intramembranei140 – 1467Pore-forming; By similarity
Topological domaini147 – 1559Extracellular By similarity
Transmembranei156 – 17722Helical; Name=M2; By similarityAdd
BLAST
Topological domaini178 – 391214Cytoplasmic By similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 391391ATP-sensitive inward rectifier potassium channel 1PRO_0000154919Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei44 – 441Phosphoserine; by SGK1 By similarity
Glycosylationi117 – 1171N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane By similarity.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP35560.

PTM databases

PhosphoSiteiP35560.

Expressioni

Tissue specificityi

Mainly in kidney (renal cortex, medulla and papilla). In the brain, found in the cortex and the hippocampus.

Gene expression databases

GenevestigatoriP35560.

Interactioni

Subunit structurei

Interacts with SGK1 and SLC9A3R2/NHERF2 By similarity.

Protein-protein interaction databases

BioGridi246676. 1 interaction.
MINTiMINT-214902.
STRINGi10116.ENSRNOP00000043570.

Structurei

3D structure databases

ProteinModelPortaliP35560.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi141 – 1466Selectivity filter By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG247934.
HOGENOMiHOG000237326.
HOVERGENiHBG006178.
KOiK04995.
PhylomeDBiP35560.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003268. K_chnl_inward-rec_Kir1.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF6. PTHR11767:SF6. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01321. KIR11CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform A (identifier: P35560-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGASERSVFR VLIRALTERM FKHLRRWFIT HIFGRSRQRA RLVSKEGRCN    50
IEFGNVDAQS RFIFFVDIWT TVLDLKWRYK MTVFITAFLG SWFLFGLLWY 100
VVAYVHKDLP EFYPPDNRTP CVENINGMTS AFLFSLETQV TIGYGFRFVT 150
EQCATAIFLL IFQSILGVII NSFMCGAILA KISRPKKRAK TITFSKNAVI 200
SKRGGKLCLL IRVANLRKSL LIGSHIYGKL LKTTITPEGE TIILDQTNIN 250
FVVDAGNENL FFISPLTIYH IIDHNSPFFH MAAETLSQQD FELVVFLDGT 300
VESTSATCQV RTSYVPEEVL WGYRFVPIVS KTKEGKYRVD FHNFGKTVEV 350
ETPHCAMCLY NEKDARARMK RGYDNPNFVL SEVDETDDTQ M 391
Length:391
Mass (Da):44,963
Last modified:June 1, 1994 - v1
Checksum:i896FD0FD128F85D0
GO
Isoform B (identifier: P35560-2) [UniParc]FASTAAdd to Basket

Also known as: ROMK2

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.

Show »
Length:372
Mass (Da):42,790
Checksum:i413C456ED8945C98
GO
Isoform C (identifier: P35560-3) [UniParc]FASTAAdd to Basket

Also known as: ROMK3

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MGASERSVFRVL → MVSELSIPSIPTGVAGLSK

Show »
Length:398
Mass (Da):45,498
Checksum:iBF9FAC45A8C17863
GO
Isoform D (identifier: P35560-4) [UniParc]FASTAAdd to Basket

Also known as: 1.1, ROMK1.1

The sequence of this isoform differs from the canonical sequence as follows:
     79-124: YKMTVFITAF...PDNRTPCVEN → DSSDHRLRIQ...HVWCHFSQDL
     125-391: Missing.

Show »
Length:124
Mass (Da):15,081
Checksum:i80711AA2CC30B94C
GO
Isoform E (identifier: P35560-5) [UniParc]FASTAAdd to Basket

Also known as: 3.1, ROMK3.1

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MGASERSVFRVL → MVSELSIPSIPTGVAGLSK
     79-124: YKMTVFITAF...PDNRTPCVEN → DSSDHRLRIQ...HVWCHFSQDL
     125-391: Missing.

Show »
Length:131
Mass (Da):15,616
Checksum:iA11E86FDBA108C45
GO
Isoform F (identifier: P35560-6) [UniParc]FASTAAdd to Basket

Also known as: 6.1, ROMK6.1

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.
     79-124: YKMTVFITAF...PDNRTPCVEN → DSSDHRLRIQ...HVWCHFSQDL
     125-391: Missing.

Show »
Length:105
Mass (Da):12,908
Checksum:i1D01BE0092A6EE7D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1919Missing in isoform B and isoform F. VSP_002799Add
BLAST
Alternative sequencei1 – 1212MGASE…VFRVL → MVSELSIPSIPTGVAGLSK in isoform C and isoform E. VSP_002800Add
BLAST
Alternative sequencei79 – 12446YKMTV…PCVEN → DSSDHRLRIQVCDRTVRHCH FPAYLPVYSWSDHQFLHVWC HFSQDL in isoform D, isoform E and isoform F. VSP_002801Add
BLAST
Alternative sequencei125 – 391267Missing in isoform D, isoform E and isoform F. VSP_002802Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72341 mRNA. Translation: CAA51068.1.
L29403 mRNA. Translation: AAA50378.1.
S69385 mRNA. Translation: AAB30553.1.
AF081365 mRNA. Translation: AAC34443.1.
AF081366 mRNA. Translation: AAC34444.1.
AF081367 mRNA. Translation: AAC34445.1.
AF081368 mRNA. Translation: AAC34446.1.
PIRiS30046.
RefSeqiNP_058719.1. NM_017023.1. [P35560-1]
UniGeneiRn.22609.

Genome annotation databases

GeneIDi24521.
KEGGirno:24521.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72341 mRNA. Translation: CAA51068.1 .
L29403 mRNA. Translation: AAA50378.1 .
S69385 mRNA. Translation: AAB30553.1 .
AF081365 mRNA. Translation: AAC34443.1 .
AF081366 mRNA. Translation: AAC34444.1 .
AF081367 mRNA. Translation: AAC34445.1 .
AF081368 mRNA. Translation: AAC34446.1 .
PIRi S30046.
RefSeqi NP_058719.1. NM_017023.1. [P35560-1 ]
UniGenei Rn.22609.

3D structure databases

ProteinModelPortali P35560.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246676. 1 interaction.
MINTi MINT-214902.
STRINGi 10116.ENSRNOP00000043570.

Chemistry

BindingDBi P35560.
ChEMBLi CHEMBL2146350.
GuidetoPHARMACOLOGYi 429.

PTM databases

PhosphoSitei P35560.

Proteomic databases

PaxDbi P35560.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 24521.
KEGGi rno:24521.

Organism-specific databases

CTDi 3758.
RGDi 2957. Kcnj1.

Phylogenomic databases

eggNOGi NOG247934.
HOGENOMi HOG000237326.
HOVERGENi HBG006178.
KOi K04995.
PhylomeDBi P35560.

Miscellaneous databases

NextBioi 603565.
PROi P35560.

Gene expression databases

Genevestigatori P35560.

Family and domain databases

Gene3Di 2.60.40.1400. 1 hit.
InterProi IPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003268. K_chnl_inward-rec_Kir1.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view ]
PANTHERi PTHR11767. PTHR11767. 1 hit.
PTHR11767:SF6. PTHR11767:SF6. 1 hit.
Pfami PF01007. IRK. 1 hit.
[Graphical view ]
PIRSFi PIRSF005465. GIRK_kir. 1 hit.
PRINTSi PR01321. KIR11CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMi SSF81296. SSF81296. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of an inwardly rectifying ATP-regulated potassium channel."
    Ho K., Nichols C.G., Lederer W.J., Lytton J., Vassilev P.M., Kanazirska M.V., Hebert S.C.
    Nature 362:31-38(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "Primary structure and functional properties of an epithelial K channel."
    Zhou H., Tate S.S., Palmer L.G.
    Am. J. Physiol. 266:C809-C824(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  3. "Splicing of a retained intron within ROMK K+ channel RNA generates a novel set of isoforms in rat kidney."
    Beesley A.H., Ortega B., White S.J.
    Am. J. Physiol. 276:C585-C592(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS D; E AND F).
    Strain: Wistar.
    Tissue: Kidney.

Entry informationi

Entry nameiKCNJ1_RAT
AccessioniPrimary (citable) accession number: P35560
Secondary accession number(s): O88639, O88640, Q9QUR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi