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P35560

- KCNJ1_RAT

UniProt

P35560 - KCNJ1_RAT

Protein

ATP-sensitive inward rectifier potassium channel 1

Gene

Kcnj1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium.

    Enzyme regulationi

    Inhibited by WNK3.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei171 – 1711Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi223 – 2308ATPSequence Analysis

    GO - Molecular functioni

    1. ATP-activated inward rectifier potassium channel activity Source: RGD
    2. ATP binding Source: UniProtKB-KW
    3. inward rectifier potassium channel activity Source: RGD
    4. peptide binding Source: RGD
    5. potassium ion binding Source: RGD

    GO - Biological processi

    1. cellular response to magnesium ion Source: RGD
    2. negative regulation of apoptotic process Source: RGD
    3. potassium ion transmembrane transport Source: GOC
    4. potassium ion transport Source: RGD
    5. response to potassium ion Source: RGD

    Keywords - Molecular functioni

    Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, Potassium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-sensitive inward rectifier potassium channel 1
    Alternative name(s):
    ATP-regulated potassium channel ROM-K
    Inward rectifier K(+) channel Kir1.1
    KAB-1
    Potassium channel, inwardly rectifying subfamily J member 1
    Gene namesi
    Name:Kcnj1
    Synonyms:Romk1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2957. Kcnj1.

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein
    Note: Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane.By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 391391ATP-sensitive inward rectifier potassium channel 1PRO_0000154919Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei44 – 441Phosphoserine; by SGK1By similarity
    Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP35560.

    PTM databases

    PhosphoSiteiP35560.

    Expressioni

    Tissue specificityi

    Mainly in kidney (renal cortex, medulla and papilla). In the brain, found in the cortex and the hippocampus.

    Gene expression databases

    GenevestigatoriP35560.

    Interactioni

    Subunit structurei

    Interacts with SGK1 and SLC9A3R2/NHERF2.By similarity

    Protein-protein interaction databases

    BioGridi246676. 1 interaction.
    MINTiMINT-214902.
    STRINGi10116.ENSRNOP00000043570.

    Structurei

    3D structure databases

    ProteinModelPortaliP35560.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7777CytoplasmicBy similarityAdd
    BLAST
    Topological domaini103 – 12725ExtracellularBy similarityAdd
    BLAST
    Topological domaini147 – 1559ExtracellularBy similarity
    Topological domaini178 – 391214CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei128 – 13912Helical; Pore-forming; Name=H5By similarityAdd
    BLAST
    Intramembranei140 – 1467Pore-formingBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei78 – 10225Helical; Name=M1By similarityAdd
    BLAST
    Transmembranei156 – 17722Helical; Name=M2By similarityAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi141 – 1466Selectivity filterBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG247934.
    HOGENOMiHOG000237326.
    HOVERGENiHBG006178.
    KOiK04995.
    PhylomeDBiP35560.

    Family and domain databases

    Gene3Di2.60.40.1400. 1 hit.
    InterProiIPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003268. K_chnl_inward-rec_Kir1.1.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view]
    PANTHERiPTHR11767. PTHR11767. 1 hit.
    PTHR11767:SF6. PTHR11767:SF6. 1 hit.
    PfamiPF01007. IRK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005465. GIRK_kir. 1 hit.
    PRINTSiPR01321. KIR11CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequences (6)i

    Sequence statusi: Complete.

    This entry describes 6 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: P35560-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGASERSVFR VLIRALTERM FKHLRRWFIT HIFGRSRQRA RLVSKEGRCN    50
    IEFGNVDAQS RFIFFVDIWT TVLDLKWRYK MTVFITAFLG SWFLFGLLWY 100
    VVAYVHKDLP EFYPPDNRTP CVENINGMTS AFLFSLETQV TIGYGFRFVT 150
    EQCATAIFLL IFQSILGVII NSFMCGAILA KISRPKKRAK TITFSKNAVI 200
    SKRGGKLCLL IRVANLRKSL LIGSHIYGKL LKTTITPEGE TIILDQTNIN 250
    FVVDAGNENL FFISPLTIYH IIDHNSPFFH MAAETLSQQD FELVVFLDGT 300
    VESTSATCQV RTSYVPEEVL WGYRFVPIVS KTKEGKYRVD FHNFGKTVEV 350
    ETPHCAMCLY NEKDARARMK RGYDNPNFVL SEVDETDDTQ M 391
    Length:391
    Mass (Da):44,963
    Last modified:June 1, 1994 - v1
    Checksum:i896FD0FD128F85D0
    GO
    Isoform B (identifier: P35560-2) [UniParc]FASTAAdd to Basket

    Also known as: ROMK2

    The sequence of this isoform differs from the canonical sequence as follows:
         1-19: Missing.

    Show »
    Length:372
    Mass (Da):42,790
    Checksum:i413C456ED8945C98
    GO
    Isoform C (identifier: P35560-3) [UniParc]FASTAAdd to Basket

    Also known as: ROMK3

    The sequence of this isoform differs from the canonical sequence as follows:
         1-12: MGASERSVFRVL → MVSELSIPSIPTGVAGLSK

    Show »
    Length:398
    Mass (Da):45,498
    Checksum:iBF9FAC45A8C17863
    GO
    Isoform D (identifier: P35560-4) [UniParc]FASTAAdd to Basket

    Also known as: 1.1, ROMK1.1

    The sequence of this isoform differs from the canonical sequence as follows:
         79-124: YKMTVFITAF...PDNRTPCVEN → DSSDHRLRIQ...HVWCHFSQDL
         125-391: Missing.

    Show »
    Length:124
    Mass (Da):15,081
    Checksum:i80711AA2CC30B94C
    GO
    Isoform E (identifier: P35560-5) [UniParc]FASTAAdd to Basket

    Also known as: 3.1, ROMK3.1

    The sequence of this isoform differs from the canonical sequence as follows:
         1-12: MGASERSVFRVL → MVSELSIPSIPTGVAGLSK
         79-124: YKMTVFITAF...PDNRTPCVEN → DSSDHRLRIQ...HVWCHFSQDL
         125-391: Missing.

    Show »
    Length:131
    Mass (Da):15,616
    Checksum:iA11E86FDBA108C45
    GO
    Isoform F (identifier: P35560-6) [UniParc]FASTAAdd to Basket

    Also known as: 6.1, ROMK6.1

    The sequence of this isoform differs from the canonical sequence as follows:
         1-19: Missing.
         79-124: YKMTVFITAF...PDNRTPCVEN → DSSDHRLRIQ...HVWCHFSQDL
         125-391: Missing.

    Show »
    Length:105
    Mass (Da):12,908
    Checksum:i1D01BE0092A6EE7D
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1919Missing in isoform B and isoform F. 2 PublicationsVSP_002799Add
    BLAST
    Alternative sequencei1 – 1212MGASE…VFRVL → MVSELSIPSIPTGVAGLSK in isoform C and isoform E. 1 PublicationVSP_002800Add
    BLAST
    Alternative sequencei79 – 12446YKMTV…PCVEN → DSSDHRLRIQVCDRTVRHCH FPAYLPVYSWSDHQFLHVWC HFSQDL in isoform D, isoform E and isoform F. 1 PublicationVSP_002801Add
    BLAST
    Alternative sequencei125 – 391267Missing in isoform D, isoform E and isoform F. 1 PublicationVSP_002802Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72341 mRNA. Translation: CAA51068.1.
    L29403 mRNA. Translation: AAA50378.1.
    S69385 mRNA. Translation: AAB30553.1.
    AF081365 mRNA. Translation: AAC34443.1.
    AF081366 mRNA. Translation: AAC34444.1.
    AF081367 mRNA. Translation: AAC34445.1.
    AF081368 mRNA. Translation: AAC34446.1.
    PIRiS30046.
    RefSeqiNP_058719.1. NM_017023.1. [P35560-1]
    UniGeneiRn.22609.

    Genome annotation databases

    GeneIDi24521.
    KEGGirno:24521.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72341 mRNA. Translation: CAA51068.1 .
    L29403 mRNA. Translation: AAA50378.1 .
    S69385 mRNA. Translation: AAB30553.1 .
    AF081365 mRNA. Translation: AAC34443.1 .
    AF081366 mRNA. Translation: AAC34444.1 .
    AF081367 mRNA. Translation: AAC34445.1 .
    AF081368 mRNA. Translation: AAC34446.1 .
    PIRi S30046.
    RefSeqi NP_058719.1. NM_017023.1. [P35560-1 ]
    UniGenei Rn.22609.

    3D structure databases

    ProteinModelPortali P35560.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246676. 1 interaction.
    MINTi MINT-214902.
    STRINGi 10116.ENSRNOP00000043570.

    Chemistry

    BindingDBi P35560.
    ChEMBLi CHEMBL2146350.
    GuidetoPHARMACOLOGYi 429.

    PTM databases

    PhosphoSitei P35560.

    Proteomic databases

    PaxDbi P35560.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24521.
    KEGGi rno:24521.

    Organism-specific databases

    CTDi 3758.
    RGDi 2957. Kcnj1.

    Phylogenomic databases

    eggNOGi NOG247934.
    HOGENOMi HOG000237326.
    HOVERGENi HBG006178.
    KOi K04995.
    PhylomeDBi P35560.

    Miscellaneous databases

    NextBioi 603565.
    PROi P35560.

    Gene expression databases

    Genevestigatori P35560.

    Family and domain databases

    Gene3Di 2.60.40.1400. 1 hit.
    InterProi IPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003268. K_chnl_inward-rec_Kir1.1.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view ]
    PANTHERi PTHR11767. PTHR11767. 1 hit.
    PTHR11767:SF6. PTHR11767:SF6. 1 hit.
    Pfami PF01007. IRK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005465. GIRK_kir. 1 hit.
    PRINTSi PR01321. KIR11CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of an inwardly rectifying ATP-regulated potassium channel."
      Ho K., Nichols C.G., Lederer W.J., Lytton J., Vassilev P.M., Kanazirska M.V., Hebert S.C.
      Nature 362:31-38(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    2. "Primary structure and functional properties of an epithelial K channel."
      Zhou H., Tate S.S., Palmer L.G.
      Am. J. Physiol. 266:C809-C824(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    3. "Splicing of a retained intron within ROMK K+ channel RNA generates a novel set of isoforms in rat kidney."
      Beesley A.H., Ortega B., White S.J.
      Am. J. Physiol. 276:C585-C592(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS D; E AND F).
      Strain: Wistar.
      Tissue: Kidney.

    Entry informationi

    Entry nameiKCNJ1_RAT
    AccessioniPrimary (citable) accession number: P35560
    Secondary accession number(s): O88639, O88640, Q9QUR5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3