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P35560 (IRK1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-sensitive inward rectifier potassium channel 1
Alternative name(s):
ATP-regulated potassium channel ROM-K
Inward rectifier K(+) channel Kir1.1
KAB-1
Potassium channel, inwardly rectifying subfamily J member 1
Gene names
Name:Kcnj1
Synonyms:Romk1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length391 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium.

Enzyme regulation

Inhibited by WNK3 By similarity.

Subunit structure

Interacts with SGK1 and SLC9A3R2/NHERF2 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Note: Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane By similarity.

Tissue specificity

Mainly in kidney (renal cortex, medulla and papilla). In the brain, found in the cortex and the hippocampus.

Post-translational modification

Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane By similarity.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ1 subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Transport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
Potassium
   Molecular functionIon channel
Voltage-gated channel
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to magnesium ion

Inferred from direct assay PubMed 21030597. Source: RGD

negative regulation of apoptotic process

Inferred from mutant phenotype PubMed 22811560. Source: RGD

potassium ion transmembrane transport

Inferred from direct assay PubMed 7611454. Source: GOC

potassium ion transport

Inferred from mutant phenotype PubMed 16906771. Source: RGD

response to potassium ion

Inferred from expression pattern PubMed 20702602. Source: RGD

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from direct assay PubMed 16906771. Source: RGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-activated inward rectifier potassium channel activity

Inferred from direct assay PubMed 7611454. Source: RGD

inward rectifier potassium channel activity

Inferred from mutant phenotype PubMed 16906771. Source: RGD

peptide binding

Inferred from direct assay PubMed 16906771. Source: RGD

potassium ion binding

Inferred from mutant phenotype PubMed 16906771. Source: RGD

Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: P35560-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: P35560-2)

Also known as: ROMK2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.
Isoform C (identifier: P35560-3)

Also known as: ROMK3;

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MGASERSVFRVL → MVSELSIPSIPTGVAGLSK
Isoform D (identifier: P35560-4)

Also known as: 1.1; ROMK1.1;

The sequence of this isoform differs from the canonical sequence as follows:
     79-124: YKMTVFITAF...PDNRTPCVEN → DSSDHRLRIQ...HVWCHFSQDL
     125-391: Missing.
Isoform E (identifier: P35560-5)

Also known as: 3.1; ROMK3.1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MGASERSVFRVL → MVSELSIPSIPTGVAGLSK
     79-124: YKMTVFITAF...PDNRTPCVEN → DSSDHRLRIQ...HVWCHFSQDL
     125-391: Missing.
Isoform F (identifier: P35560-6)

Also known as: 6.1; ROMK6.1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.
     79-124: YKMTVFITAF...PDNRTPCVEN → DSSDHRLRIQ...HVWCHFSQDL
     125-391: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 391391ATP-sensitive inward rectifier potassium channel 1
PRO_0000154919

Regions

Topological domain1 – 7777Cytoplasmic By similarity
Transmembrane78 – 10225Helical; Name=M1; By similarity
Topological domain103 – 12725Extracellular By similarity
Intramembrane128 – 13912Helical; Pore-forming; Name=H5; By similarity
Intramembrane140 – 1467Pore-forming; By similarity
Topological domain147 – 1559Extracellular By similarity
Transmembrane156 – 17722Helical; Name=M2; By similarity
Topological domain178 – 391214Cytoplasmic By similarity
Nucleotide binding223 – 2308ATP Potential
Motif141 – 1466Selectivity filter By similarity

Sites

Site1711Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Amino acid modifications

Modified residue441Phosphoserine; by SGK1 By similarity
Glycosylation1171N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 1919Missing in isoform B and isoform F.
VSP_002799
Alternative sequence1 – 1212MGASE…VFRVL → MVSELSIPSIPTGVAGLSK in isoform C and isoform E.
VSP_002800
Alternative sequence79 – 12446YKMTV…PCVEN → DSSDHRLRIQVCDRTVRHCH FPAYLPVYSWSDHQFLHVWC HFSQDL in isoform D, isoform E and isoform F.
VSP_002801
Alternative sequence125 – 391267Missing in isoform D, isoform E and isoform F.
VSP_002802

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 896FD0FD128F85D0

FASTA39144,963
        10         20         30         40         50         60 
MGASERSVFR VLIRALTERM FKHLRRWFIT HIFGRSRQRA RLVSKEGRCN IEFGNVDAQS 

        70         80         90        100        110        120 
RFIFFVDIWT TVLDLKWRYK MTVFITAFLG SWFLFGLLWY VVAYVHKDLP EFYPPDNRTP 

       130        140        150        160        170        180 
CVENINGMTS AFLFSLETQV TIGYGFRFVT EQCATAIFLL IFQSILGVII NSFMCGAILA 

       190        200        210        220        230        240 
KISRPKKRAK TITFSKNAVI SKRGGKLCLL IRVANLRKSL LIGSHIYGKL LKTTITPEGE 

       250        260        270        280        290        300 
TIILDQTNIN FVVDAGNENL FFISPLTIYH IIDHNSPFFH MAAETLSQQD FELVVFLDGT 

       310        320        330        340        350        360 
VESTSATCQV RTSYVPEEVL WGYRFVPIVS KTKEGKYRVD FHNFGKTVEV ETPHCAMCLY 

       370        380        390 
NEKDARARMK RGYDNPNFVL SEVDETDDTQ M 

« Hide

Isoform B (ROMK2) [UniParc].

Checksum: 413C456ED8945C98
Show »

FASTA37242,790
Isoform C (ROMK3) [UniParc].

Checksum: BF9FAC45A8C17863
Show »

FASTA39845,498
Isoform D (1.1) (ROMK1.1) [UniParc].

Checksum: 80711AA2CC30B94C
Show »

FASTA12415,081
Isoform E (3.1) (ROMK3.1) [UniParc].

Checksum: A11E86FDBA108C45
Show »

FASTA13115,616
Isoform F (6.1) (ROMK6.1) [UniParc].

Checksum: 1D01BE0092A6EE7D
Show »

FASTA10512,908

References

[1]"Cloning and expression of an inwardly rectifying ATP-regulated potassium channel."
Ho K., Nichols C.G., Lederer W.J., Lytton J., Vassilev P.M., Kanazirska M.V., Hebert S.C.
Nature 362:31-38(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kidney.
[2]"Primary structure and functional properties of an epithelial K channel."
Zhou H., Tate S.S., Palmer L.G.
Am. J. Physiol. 266:C809-C824(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
Strain: Sprague-Dawley.
Tissue: Kidney.
[3]"Splicing of a retained intron within ROMK K+ channel RNA generates a novel set of isoforms in rat kidney."
Beesley A.H., Ortega B., White S.J.
Am. J. Physiol. 276:C585-C592(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS D; E AND F).
Strain: Wistar.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X72341 mRNA. Translation: CAA51068.1.
L29403 mRNA. Translation: AAA50378.1.
S69385 mRNA. Translation: AAB30553.1.
AF081365 mRNA. Translation: AAC34443.1.
AF081366 mRNA. Translation: AAC34444.1.
AF081367 mRNA. Translation: AAC34445.1.
AF081368 mRNA. Translation: AAC34446.1.
PIRS30046.
RefSeqNP_058719.1. NM_017023.1.
UniGeneRn.22609.

3D structure databases

ProteinModelPortalP35560.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid246676. 1 interaction.
MINTMINT-214902.
STRING10116.ENSRNOP00000043570.

Chemistry

BindingDBP35560.
ChEMBLCHEMBL2146350.
GuidetoPHARMACOLOGY429.

PTM databases

PhosphoSiteP35560.

Proteomic databases

PaxDbP35560.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24521.
KEGGrno:24521.

Organism-specific databases

CTD3758.
RGD2957. Kcnj1.

Phylogenomic databases

eggNOGNOG247934.
HOGENOMHOG000237326.
HOVERGENHBG006178.
KOK04995.
PhylomeDBP35560.

Gene expression databases

GenevestigatorP35560.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003268. K_chnl_inward-rec_Kir1.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF6. PTHR11767:SF6. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01321. KIR11CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

NextBio603565.
PROP35560.

Entry information

Entry nameIRK1_RAT
AccessionPrimary (citable) accession number: P35560
Secondary accession number(s): O88639, O88640, Q9QUR5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families