##gff-version 3 P35559 UniProtKB Chain 1 1019 . . . ID=PRO_0000074406;Note=Insulin-degrading enzyme P35559 UniProtKB Motif 853 858 . . . Note=SlyX motif;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9JHR7 P35559 UniProtKB Active site 111 111 . . . Note=Proton acceptor;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU10096 P35559 UniProtKB Binding site 108 108 . . . Ontology_term=ECO:0000269,ECO:0007744;evidence=ECO:0000269|PubMed:21731629,ECO:0007744|PDB:3P7L;Dbxref=PMID:21731629 P35559 UniProtKB Binding site 112 112 . . . Ontology_term=ECO:0000269,ECO:0007744;evidence=ECO:0000269|PubMed:21731629,ECO:0007744|PDB:3P7L;Dbxref=PMID:21731629 P35559 UniProtKB Binding site 189 189 . . . Ontology_term=ECO:0000269,ECO:0007744;evidence=ECO:0000269|PubMed:21731629,ECO:0007744|PDB:3P7L;Dbxref=PMID:21731629 P35559 UniProtKB Binding site 359 363 . . . Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P14735 P35559 UniProtKB Binding site 429 429 . . . Ontology_term=ECO:0000269,ECO:0007744;evidence=ECO:0000269|PubMed:22049080,ECO:0007744|PDB:3TUV;Dbxref=PMID:22049080 P35559 UniProtKB Binding site 895 901 . . . Ontology_term=ECO:0000269,ECO:0007744;evidence=ECO:0000269|PubMed:22049080,ECO:0007744|PDB:3TUV;Dbxref=PMID:22049080 P35559 UniProtKB Modified residue 192 192 . . . Note=N6-succinyllysine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9JHR7 P35559 UniProtKB Modified residue 697 697 . . . Note=N6-succinyllysine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9JHR7 P35559 UniProtKB Mutagenesis 111 111 . . . Note=Expected to abolish catalytic activity. E->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21731629;Dbxref=PMID:21731629 P35559 UniProtKB Mutagenesis 111 111 . . . Note=Abolishes catalytic activity. No effect on substrate binding. E->V;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:14527953;Dbxref=PMID:14527953 P35559 UniProtKB Mutagenesis 112 112 . . . Note=Abolishes catalytic activity. Decreases substrate binding affinity. H->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:14527953;Dbxref=PMID:14527953 P35559 UniProtKB Mutagenesis 360 360 . . . Note=Strongly reduced catalytic activity. Strongly reduced stimulation of activity by ATP. V->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21731629;Dbxref=PMID:21731629 P35559 UniProtKB Mutagenesis 374 374 . . . Note=Strongly reduced catalytic activity. Abolishes stimulation of activity by ATP. I->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21731629;Dbxref=PMID:21731629 P35559 UniProtKB Mutagenesis 429 429 . . . Note=Greatly decreased activation by polyphosphate anions. R->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22049080;Dbxref=PMID:22049080 P35559 UniProtKB Mutagenesis 609 609 . . . Note=Strongly reduced catalytic activity. Abolishes stimulation of activity by ATP. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21731629;Dbxref=PMID:21731629 P35559 UniProtKB Mutagenesis 898 898 . . . Note=Greatly decreased activation by polyphosphate anions%2C and deficient in activation by small peptides%3B when associated with A-899 and A-901. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22049080;Dbxref=PMID:22049080 P35559 UniProtKB Mutagenesis 899 899 . . . Note=Greatly decreased activation by polyphosphate anions%2C and deficient in activation by small peptides%3B when associated with A-898 and A-901. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22049080;Dbxref=PMID:22049080 P35559 UniProtKB Mutagenesis 901 901 . . . Note=Greatly decreased activation by polyphosphate anions%2C and deficient in activation by small peptides%3B when associated with A-898 and A-899. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22049080;Dbxref=PMID:22049080 P35559 UniProtKB Beta strand 47 51 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 63 69 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 74 79 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 84 93 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 96 98 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 101 103 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7O P35559 UniProtKB Helix 106 114 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 118 121 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 126 132 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Turn 133 135 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 137 142 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 147 154 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 155 157 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 158 166 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 167 169 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 176 194 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 197 206 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 211 213 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3TUV P35559 UniProtKB Helix 214 216 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 223 226 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 228 232 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 237 248 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 251 253 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 254 262 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 264 275 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 295 297 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 298 305 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 307 309 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3TUV P35559 UniProtKB Beta strand 312 320 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 323 325 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Turn 326 328 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 330 338 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 346 352 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 355 367 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 370 379 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 381 385 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 387 404 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 408 423 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 430 440 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Turn 441 443 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 446 448 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Turn 449 454 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 461 470 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 473 475 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 477 481 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 483 485 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Turn 494 496 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 499 504 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 507 514 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 549 553 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 555 563 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 570 579 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 581 583 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 584 586 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7O P35559 UniProtKB Helix 587 613 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 616 622 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 624 635 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 638 650 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 656 672 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 673 675 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 678 690 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 691 693 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 697 704 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 709 721 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 722 732 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 735 753 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 760 762 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 775 782 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 787 799 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 802 823 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Turn 824 827 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 833 840 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 843 854 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 856 875 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 879 894 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 900 912 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 920 929 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 933 943 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 952 959 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Beta strand 990 993 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L P35559 UniProtKB Helix 995 1000 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3P7L