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Protein

rRNA 2'-O-methyltransferase fibrillarin

Gene

fib1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-glutamine-[histone] = S-adenosyl-L-homocysteine + N(5)-methyl-L-glutamine-[histone].

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Ribonucleoprotein, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-SPO-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
rRNA 2'-O-methyltransferase fibrillarin (EC:2.1.1.-)
Alternative name(s):
Histone-glutamine methyltransferase
Gene namesi
Name:fib1
Synonyms:fib
ORF Names:SPBC2D10.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC2D10.10c.
PomBaseiSPBC2D10.10c. fib1.

Subcellular locationi

GO - Cellular componenti

  • box C/D snoRNP complex Source: GO_Central
  • Cajal body Source: GO_Central
  • mitotic spindle Source: PomBase
  • nucleolus Source: PomBase
  • nucleus Source: PomBase
  • small-subunit processome Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001485261 – 305rRNA 2'-O-methyltransferase fibrillarinAdd BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei111Phosphoserine1 Publication1
Modified residuei114Phosphoserine1 Publication1

Post-translational modificationi

By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35551.
PRIDEiP35551.

PTM databases

iPTMnetiP35551.

Interactioni

Subunit structurei

Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles.By similarity

Protein-protein interaction databases

BioGridi277007. 10 interactors.
MINTiMINT-4688598.

Structurei

3D structure databases

ProteinModelPortaliP35551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni160 – 161S-adenosyl-L-methionine bindingBy similarity2
Regioni179 – 180S-adenosyl-L-methionine bindingBy similarity2
Regioni204 – 205S-adenosyl-L-methionine bindingBy similarity2
Regioni224 – 227S-adenosyl-L-methionine bindingBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi6 – 70DMA/Gly-richAdd BLAST65

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000106741.
InParanoidiP35551.
KOiK14563.
OMAiMREFIDS.
OrthoDBiEOG092C4CCH.
PhylomeDBiP35551.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00351. RNA_methyltransf_FlpA. 1 hit.
InterProiIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFiPIRSF006540. Nop17p. 1 hit.
PRINTSiPR00052. FIBRILLARIN.
SMARTiSM01206. Fibrillarin. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00566. FIBRILLARIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYTPGSRGG RGGSRGGRGG FNGGRGGFGG GRGGARGGGR GGARGGRGGR
60 70 80 90 100
GGARGGRGGS SGGRGGAKGG AKVIIEPHRH AGVFIARGKE DLLVTRNLVP
110 120 130 140 150
GESVYNEKRI SVDSPDGTKV EYRVWNPFRS KLAAGILGGL DNIYIKPGAR
160 170 180 190 200
VLYLGAANGT SVSHVADVVG PEGLVYAVEF SHRSGRDLLN MAKKRTNVIP
210 220 230 240 250
IVEDARHVQK YRMLVGMVDV VFADVAQPDQ ARIVALNAAA FLKNEGGVVI
260 270 280 290 300
SVKASCIDST ADAAVVFARE VKKMQEEKIK PQEQLTLEPY ERDHCIIVGK

YLRHQ
Length:305
Mass (Da):32,040
Last modified:June 1, 1994 - v1
Checksum:i5AE6E8B37EC33331
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69930 Genomic DNA. Translation: CAA49550.1.
CU329671 Genomic DNA. Translation: CAA21168.1.
AB027941 Genomic DNA. Translation: BAA87245.1.
PIRiS33690.
RefSeqiNP_596229.1. NM_001022149.2.

Genome annotation databases

EnsemblFungiiSPBC2D10.10c.1; SPBC2D10.10c.1:pep; SPBC2D10.10c.
GeneIDi2540479.
KEGGispo:SPBC2D10.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69930 Genomic DNA. Translation: CAA49550.1.
CU329671 Genomic DNA. Translation: CAA21168.1.
AB027941 Genomic DNA. Translation: BAA87245.1.
PIRiS33690.
RefSeqiNP_596229.1. NM_001022149.2.

3D structure databases

ProteinModelPortaliP35551.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277007. 10 interactors.
MINTiMINT-4688598.

PTM databases

iPTMnetiP35551.

Proteomic databases

MaxQBiP35551.
PRIDEiP35551.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC2D10.10c.1; SPBC2D10.10c.1:pep; SPBC2D10.10c.
GeneIDi2540479.
KEGGispo:SPBC2D10.10c.

Organism-specific databases

EuPathDBiFungiDB:SPBC2D10.10c.
PomBaseiSPBC2D10.10c. fib1.

Phylogenomic databases

HOGENOMiHOG000106741.
InParanoidiP35551.
KOiK14563.
OMAiMREFIDS.
OrthoDBiEOG092C4CCH.
PhylomeDBiP35551.

Enzyme and pathway databases

ReactomeiR-SPO-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Miscellaneous databases

PROiP35551.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00351. RNA_methyltransf_FlpA. 1 hit.
InterProiIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFiPIRSF006540. Nop17p. 1 hit.
PRINTSiPR00052. FIBRILLARIN.
SMARTiSM01206. Fibrillarin. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00566. FIBRILLARIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFBRL_SCHPO
AccessioniPrimary (citable) accession number: P35551
Secondary accession number(s): Q9UTX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.